Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000149.1:
Cluster 1Polyketide190501

BGC0000149, spirangien biosynthetic gene cluster from Sorangium cellulosum. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Spirangien A1
SMILES string: Copy to clipboard
Molecular formula: C41H66O9
Exact molecular mass: 703.4779 Da ([M+H]+)
Molecular activity: Cytotoxic

Class-specific details

Biosynthetic class(es):
Polyketide
Polyketide subclass:
Other (cyclic)
Polyketide synthase subclass:
Modular type I
Starter unit:
Acetyl-CoA
Thioesterase type:
Type I
Release / cyclization type:
Hydrolysis

Modular polyketide synthases:
spiD (CAL58681.1)
Module 1
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Module 2
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group

spiE (CAL58682.1)
Module 3
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Module 4
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group

spiF (CAL58683.1)
Module 5
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Module 6
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group

spiG (CAL58684.1)
Module 7
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Module 8
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group

spiH (CAL58685.1)
Module 9
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Module 10
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Module 11
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group

spiI (CAL58686.1)
Module 12
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Module 13
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group

spiJ (CAL58687.1)
Module 14
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Module 15
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group

Gene cluster description

spirangien (BGC0000149). Gene Cluster 1. Biosynthetic class = Polyketide. GenBank AM407731, positions 1-90501. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:No
Contact for this cluster:Daniel Krug (HIPS)

Literature references

1. Frank B et al. (2007) Spiroketal polyketide formation in Sorangium: identification and analysis of the biosynthetic gene cluster for the highly cytotoxic spirangienes. Chem Biol 14(2):221-33. doi: 10.1016/j.chembiol.2006.11.013.