BGC0000151: stambomycin A biosynthetic gene cluster from Streptomyces ambofaciens ATCC 23877
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Location: 468,938 - 638,587 nt. (total: 169,650 nt).
This entry is originally from NCBI GenBank AM238664.2.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
KnownClusterBlast
NRPS/PKS domains
General information about the BGC
MIBiG accession BGC0000151
Short description stambomycin A biosynthetic gene cluster from Streptomyces ambofaciens ATCC 23877
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "methylmalonyl-CoA is used to create the propionyl starter unit in Stambomycin biosynthesis"
Biosynthetic class(es)
  • PKS (Type I)
  • saccharide (hybrid/tailoring)
Loci
AM238664.2
488938 - 634937
via Knock-out studies, Gene expression correlated with compound production
Compounds
  • stambomycin A
  • stambomycin B
  • stambomycin C
  • stambomycin D
Species Streptomyces ambofaciens ATCC 23877 [taxonomy]
References
Chemical products information
stambomycin A Evidence:
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C74H135N1O21
stambomycin B Evidence:
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C74H135N1O21
stambomycin C Evidence:
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C73H133N1O21
stambomycin D Evidence:
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C73H133N1O21
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • SAMR0465
  • CAJ88175.1
 488938 - 513402 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
  • Other in vivo study
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  • SAMR0466
  • CAJ88176.1
 513419 - 524404 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
  • Other in vivo study
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  • SAMR0467
  • CAJ88177.1
 524441 - 541756 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
  • Knock-out Other in vivo study
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  • SAMR0468
  • CAJ88178.1
 541988 - 542641 (-) putative two-component system response regulator
  • Regulation
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  • SAMR0469
  • CAJ88179.1
 542631 - 543959 (-) putative two-component system sensor kinase
  • Regulation
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  • SAMR0470
  • CAJ88180.1
 543956 - 544741 (-) putative integral membrane protein
  • Transport
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  • SAMR0471
  • CAJ88181.1
 544738 - 545676 (-) putative ABC transporter, ATP-binding subunit
  • Transport
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  • SAMR0472
  • CAJ88182.1
 545796 - 546530 (-) putative TDP-N-dimethyldesosamine-N-methyltransferase
  • Tailoring
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  • SAMR0473
  • CAJ88183.1
 546527 - 547084 (-) putative 3,4 keto-isomerase
  • Tailoring
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  • SAMR0474
  • CAJ88184.1
 547197 - 566198 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
  • Other in vivo study
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  • SAMRCDS1
  • CBZ41585.1
 566203 - 576873 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
  • Other in vivo study
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  • SAMR0475
  • CAJ88185.2
 576909 - 586382 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
  • Other in vivo study
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  • SAMR0476
  • CAJ88186.1
 586814 - 597511 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
  • Other in vivo study
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  • SAMRCDS2
  • CBZ41586.1
 597514 - 602223 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
  • Other in vivo study
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  • SAMR0477
  • CAJ88187.2
 602244 - 618587 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
  • Other in vivo study
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  • SAMR0478
  • CAJ88188.1
 618781 - 620025 (-) Cytochrome P450
  • Tailoring
  • Knock-out
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  • SAMR0479
  • CAJ88189.1
 620468 - 621673 (+) Cytochrome P450
  • Tailoring
  • Knock-out
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  • SAMR0480
  • CAJ88190.1
 621765 - 622874 (-) putative aminotransferase
  • Tailoring
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  • SAMR0481
  • CAJ88191.1
 623115 - 624371 (-) Glycosyltransferase
  • Tailoring
  • Knock-out
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  • SAMR0482
  • CAJ88192.1
 624933 - 626720 (+) Putative acyl-CoA synthetase
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    • SAMR0483
    • CAJ88193.1
    		 626781 - 628379 (-) Putative carboxyl transferase
    • Precursor biosynthesis
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    • SAMR0484
    • CAJ88194.1
    		 628900 - 631776 (+) Transcriptional activator (LAL)
    • Regulation
    • Other in vivo study
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    • SAMR0485
    • CAJ88195.1
    		 632124 - 632891 (+) putative thioesterase
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      • SAMR0486
      • CAJ88196.1
      		 633066 - 634055 (-) putative dTDP-glucose 4,6-dehydratase
      • Tailoring
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      • SAMR0487
      • CAJ88197.1
      		 634065 - 634937 (-) putative glucose-1-phosphate thymidyltransferase
      • Tailoring
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      Biosynthesis information

      Biosynthetic modules

      Name
      0
      Type
      pks-modular
      Genes
      SAMR0467
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, carrier (ACP)
      Name
      1
      Type
      pks-modular
      Genes
      SAMR0467
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
      Name
      2
      Type
      pks-modular
      Genes
      SAMR0467
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      3
      Type
      pks-modular
      Genes
      SAMR0467
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      4
      Type
      pks-modular
      Genes
      SAMR0466
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, dehydratase, enoylreductase, ketoreductase, carrier (ACP)
      Name
      5
      Type
      pks-modular
      Genes
      SAMR0466
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      6
      Type
      pks-modular
      Genes
      SAMR0465
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      7
      Type
      pks-modular
      Genes
      SAMR0465
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, dehydratase, enoylreductase, ketoreductase, carrier (ACP)
      Name
      8
      Type
      pks-modular
      Genes
      SAMR0465
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      9
      Type
      pks-modular
      Genes
      SAMR0465
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      10
      Type
      pks-modular
      Genes
      SAMR0465
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      11
      Type
      pks-modular
      Genes
      SAMR0477
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, dehydratase, enoylreductase, ketoreductase, carrier (ACP)
      Name
      12
      Type
      pks-modular
      Genes
      SAMR0477
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      13
      Type
      pks-modular
      Genes
      SAMR0477
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
      Name
      14
      Type
      pks-modular
      Genes
      SAMRCDS2
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      15
      Type
      pks-modular
      Genes
      SAMR0476
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      16
      Type
      pks-modular
      Genes
      SAMR0476
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, dehydratase, enoylreductase, ketoreductase, carrier (ACP)
      Name
      17
      Type
      pks-modular
      Genes
      SAMR0475
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      18
      Type
      pks-modular
      Genes
      SAMR0475
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      19
      Type
      pks-modular
      Genes
      SAMRCDS1
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
      Name
      20
      Type
      pks-modular
      Genes
      SAMRCDS1
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
      Name
      21
      Type
      pks-modular
      Genes
      SAMR0474
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      22
      Type
      pks-modular
      Genes
      SAMR0474
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      23
      Type
      pks-modular
      Genes
      SAMR0474
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      24
      Type
      pks-modular
      Genes
      SAMR0474
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, thioesterase, carrier (ACP)
      Annotation changelog

      Entry version: 4

      Date
      Changes
      Submitters
      Reviewers
      2022-10-07
      Update chemical activity to schema version 2.11
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

      Entry version: 3

      Date
      Changes
      Submitters
      Reviewers
      2022-09-15
      Removed ketoreductase stereochemistry annotation from modules without ketoreductases
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      2022-09-15
      Sorted modules by module number
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      2022-09-15
      Corrected gene identifiers
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      2022-09-15
      Updated bioactivity data
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

      Entry version: 2

      Date
      Changes
      Submitters
      Reviewers
      2019-10-16
      Migrated from v1.4
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

      Entry version: 1

      Date
      Changes
      Submitters
      Reviewers
      2015-06-12
      Submitted
      • (ID: 7XFWG4QIVYMSCYRLUISH6MBY)
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
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