Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000151.1:
Cluster 1Polyketide / Saccharide1146000

BGC0000151, stambomycin biosynthetic gene cluster from Streptomyces ambofaciens. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: stambomycin A
SMILES string: Copy to clipboard
Molecular formula: C73H133NO22
Exact molecular mass: 1376.9391 Da ([M+H]+)
Molecular activity: Antibacterial, Other
Molecular target: unknown

Class-specific details

Biosynthetic class(es):
Polyketide / Saccharide
Polyketide subclass:
Macrolide (cyclic)
Polyketide synthase subclass:
Modular type I
Starter unit:
methylmalonate-CoA
Thioesterase type:
Type I
Release / cyclization type:
Macrolactonization

Modular polyketide synthases:
samR0477 (CAJ88187.2)
Module 11
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 12
AT specificity: None
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Scaffold-modifying domain: None
Module 13
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None

samRCDS2 (CBZ41586.1)
Module 14
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Scaffold-modifying domain: None

CAJ88186.1
Module 15
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Scaffold-modifying domain: None
Module 16
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None

samR0475 (CAJ88185.2)
Module 17
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Scaffold-modifying domain: None
Module 18
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Scaffold-modifying domain: None

samRCDS1 (CBZ41585.1)
Module 19
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 20
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None

CAJ88184.1
Module 21
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 22
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 23
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Scaffold-modifying domain: None
Module 24
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Inactive
Scaffold-modifying domain: None

CAJ88177.1
Module 0
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Scaffold-modifying domain: None
Module 1
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 2
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 3
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None

CAJ88176.1
Module 4
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 5
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Scaffold-modifying domain: None

CAJ88175.1
Module 10
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Scaffold-modifying domain: None
Module 6
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Scaffold-modifying domain: None
Module 7
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 8
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Scaffold-modifying domain: None
Module 9
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None

Gene cluster description

stambomycin (BGC0000151). Gene Cluster 1. Biosynthetic class = Polyketide/Saccharide. GenBank AM238664, positions 488938-634937. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Gene expression correlated with compound production, Sequence-based prediction
MIxS-compliance:Unknown
Comments:methylmalonyl-CoA is used to create the propionyl starter unit in Stambomycin biosynthesis
Contact for this cluster:Bertrand Aigle (University of Lorraine)

Literature references

1. Laureti L et al. (2011) Identification of a bioactive 51-membered macrolide complex by activation of a silent polyketide synthase in Streptomyces ambofaciens. Proc Natl Acad Sci U S A 108(15):6258-63. doi:
2. Song L et al. (2014) Cytochrome P450-mediated hydroxylation is required for polyketide macrolactonization in stambomycin biosynthesis. J Antibiot (Tokyo) 67(1):71-6. doi: 10.1038/ja.2013.119. Epub 2013 Nov