BGC0000151: stambomycin A biosynthetic gene cluster from Streptomyces ambofaciens ATCC 23877
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Location: 488,938 - 634,937 nt. (total: 146,000 nt).
This entry is originally from NCBI GenBank AM238664.2.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
Saccharide
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000151
Short description stambomycin A biosynthetic gene cluster from Streptomyces ambofaciens ATCC 23877
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "methylmalonyl-CoA is used to create the propionyl starter unit in Stambomycin biosynthesis"
Biosynthetic class(es)
  • Polyketide (Macrolide)
  • Saccharide (hybrid/tailoring)
Loci NCBI GenBank: AM238664.2
Compounds
  • stambomycin A
  • stambomycin B
  • stambomycin C
  • stambomycin D
Species Streptomyces ambofaciens ATCC 23877 [taxonomy]
References
Chemical products information
stambomycin A
Copy SMILES
C74H135N1O21
stambomycin B
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C74H135N1O21
stambomycin C
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C73H133N1O21
stambomycin D
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C73H133N1O21
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • SAMR0465
  • CAJ88175.1
 488938 - 513402 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
Other in vivo study
Sequence-based prediction
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  • SAMR0466
  • CAJ88176.1
 513419 - 524404 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
Other in vivo study
Sequence-based prediction
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  • SAMR0467
  • CAJ88177.1
 524441 - 541756 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
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  • SAMR0468
  • CAJ88178.1
 541988 - 542641 (-) putative two-component system response regulator
  • Regulation
Sequence-based prediction
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  • SAMR0469
  • CAJ88179.1
 542631 - 543959 (-) putative two-component system sensor kinase
  • Regulation
Sequence-based prediction
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  • SAMR0470
  • CAJ88180.1
 543956 - 544741 (-) putative integral membrane protein
  • Transport
Sequence-based prediction
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  • SAMR0471
  • CAJ88181.1
 544738 - 545676 (-) putative ABC transporter, ATP-binding subunit
  • Transport
Sequence-based prediction
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  • SAMR0472
  • CAJ88182.1
 545796 - 546530 (-) putative TDP-N-dimethyldesosamine-N-methyltransferase
  • Tailoring (Glycosylation)
Sequence-based prediction
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  • SAMR0473
  • CAJ88183.1
 546527 - 547084 (-) putative 3,4 keto-isomerase
  • Tailoring (Glycosylation)
Sequence-based prediction
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  • SAMR0474
  • CAJ88184.1
 547197 - 566198 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
Other in vivo study
Sequence-based prediction
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  • SAMRCDS1
  • CBZ41585.1
 566203 - 576873 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
Other in vivo study
Sequence-based prediction
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  • SAMR0475
  • CAJ88185.2
 576909 - 586382 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
Other in vivo study
Sequence-based prediction
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  • SAMR0476
  • CAJ88186.1
 586814 - 597511 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
Other in vivo study
Sequence-based prediction
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  • SAMRCDS2
  • CBZ41586.1
 597514 - 602223 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
Other in vivo study
Sequence-based prediction
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  • SAMR0477
  • CAJ88187.2
 602244 - 618587 (-) Type I modular polyketide synthase
  • Scaffold biosynthesis
Other in vivo study
Sequence-based prediction
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  • SAMR0478
  • CAJ88188.1
 618781 - 620025 (-) Cytochrome P450
  • Tailoring (Hydroxylation)
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  • SAMR0479
  • CAJ88189.1
 620468 - 621673 (+) Cytochrome P450
  • Tailoring (Hydroxylation)
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  • SAMR0480
  • CAJ88190.1
 621765 - 622874 (-) putative aminotransferase
  • Tailoring (Glycosylation)
Sequence-based prediction
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  • SAMR0481
  • CAJ88191.1
 623115 - 624371 (-) Glycosyltransferase
  • Tailoring (Glycosylation)
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  • SAMR0482
  • CAJ88192.1
 624933 - 626720 (+) Putative acyl-CoA synthetase
  • Unknown
Sequence-based prediction
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  • SAMR0483
  • CAJ88193.1
 626781 - 628379 (-) Putative carboxyl transferase
  • Precursor biosynthesis
Sequence-based prediction
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  • SAMR0484
  • CAJ88194.1
 628900 - 631776 (+) Transcriptional activator (LAL)
  • Regulation
Other in vivo study
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  • SAMR0485
  • CAJ88195.1
 632124 - 632891 (+) putative thioesterase
  • Other enzymatic
Sequence-based prediction
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  • SAMR0486
  • CAJ88196.1
 633066 - 634055 (-) putative dTDP-glucose 4,6-dehydratase
  • Tailoring (Glycosylation)
Sequence-based prediction
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  • SAMR0487
  • CAJ88197.1
 634065 - 634937 (-) putative glucose-1-phosphate thymidyltransferase
  • Tailoring (Glycosylation)
Sequence-based prediction
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Polyketide-specific information
Subclass Macrolide
Starter unit methylmalonate-CoA
Cyclic? yes
Release type
  • Macrolactonization
Polyketide-synthases
Genes Properties Modules
SAMR0476
+
SAMR0467
+
SAMR0465
+
SAMR0466
+
SAMR0474
+
SAMRCDS1
+
SAMR0475
+
SAMRCDS2
+
SAMR0477 		Synthase subclass: Modular type I
Module 0
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0467
Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP)
Module 1
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0467
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 2
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0467
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 3
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0467
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 4
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0466
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Enoylreductase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 5
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0466
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 6
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0465
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 7
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0465
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Enoylreductase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 8
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0465
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 9
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0465
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 10
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0465
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 11
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0477
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Enoylreductase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 12
Genes: SAMR0477
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 13
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0477
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 14
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMRCDS2
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 15
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0476
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 16
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0476
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Enoylreductase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 17
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0475
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 18
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0475
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 19
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMRCDS1
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 20
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMRCDS1
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 21
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0474
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 22
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0474
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 23
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0474
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 24
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: SAMR0474
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP), Thioesterase
KR-domain stereochemistry: Inactive
Saccharide-specific information
Subclass hybrid/tailoring
Glycosyltransferases
SAMR0481
Specificity: mycaminose
Evidence:
  • Sequence-based prediction
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: 7XFWG4QIVYMSCYRLUISH6MBY, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Sorted modules by module number
  • Corrected gene identifiers
  • Updated bioactivity data
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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