BGC0000157 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000157 (Streptomyces sp. CK4412)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	70,100	High	tautomycetin	PKS

BGC0000157 - Region 1 - T1PKS

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Location: 1 - 70,100 nt. (total: 70,100 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 70,101
Label		BGC0000157

Tailoring functions

Oxidoreductases: dehydrogenase

ABI94383.1: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Lyases: dehydratase

ABI94377.1: L-carnitine dehydratase/bile acid-inducible

smcogs: SMCOG1189: L-carnitine dehydratase/bile acid-inducible

ABI94384.1: L-carnitine dehydratase/bile acid-inducible

smcogs: SMCOG1189: L-carnitine dehydratase/bile acid-inducible

Ligases: synthase

ABI94379.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ABI94380.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000157.5	1.00	PKS	tautomycetin	Streptomyces sp. CK4412
BGC0000158.5	0.94	PKS	tautomycetin	Streptomyces griseochromogenes
BGC0000067.5	0.57	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001065.5	0.53	PKS	herboxidiene	Streptomyces chromofuscus
BGC0001495.3	0.52	PKS	actinoallolide A	Actinoallomurus fulvus
BGC0001012.3	0.52	NRPS, PKS	meridamycin	Streptomyces violaceusniger
BGC0000147.5	0.51	PKS	soraphen A	Sorangium cellulosum
BGC0001298.4	0.51	PKS	4-Z-annimycin, 4-E-annimycin	Streptomyces calvus
BGC0001051.5	0.50	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0000094.5	0.48	PKS, saccharide	methymycin	Streptomyces venezuelae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000157.5	1.00	PKS	tautomycetin	Streptomyces sp. CK4412
BGC0000158.5	0.94	PKS	tautomycetin	Streptomyces griseochromogenes
BGC0000067.5	0.57	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001065.5	0.53	PKS	herboxidiene	Streptomyces chromofuscus
BGC0001495.3	0.52	PKS	actinoallolide A	Actinoallomurus fulvus
BGC0001012.3	0.52	NRPS, PKS	meridamycin	Streptomyces violaceusniger
BGC0000147.5	0.51	PKS	soraphen A	Sorangium cellulosum
BGC0001298.4	0.51	PKS	4-Z-annimycin, 4-E-annimycin	Streptomyces calvus
BGC0001051.5	0.50	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0000094.5	0.48	PKS, saccharide	methymycin	Streptomyces venezuelae

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 70100:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-ccmal - ohmal - Me-ccmal - ccmal - Me-redmal) + (Me-redmal - redmal - pk - ohmal)
Non-colinear ordering:: ABI94379.1 + ABI94380.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ABI94379.1: mal - mmal - mal - mmal - mal - mmal

PKS_AT (645..919): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 210.8
inactive: 96.1
Methoxymalonyl-CoA: 70.4
Methylmalonyl-CoA: 53.1
Propionyl-CoA: 38.9
Isobutyryl-CoA: 31.6
Benzoyl-CoA: 30.1
Acetyl-CoA: 24.8
fatty_acid: 22.0
2-Methylbutyryl-CoA: 16.7
trans-1,2-CPDA: 15.9
Ethylmalonyl-CoA: 12.9
CHC-CoA: 9.9
3-Methylbutyryl-CoA: 0.0

PKS_AT (1622..1904): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 196.2
Ethylmalonyl-CoA: 187.2
Methoxymalonyl-CoA: 160.8
2-Methylbutyryl-CoA: 129.4
Propionyl-CoA: 105.2
trans-1,2-CPDA: 102.6
Malonyl-CoA: 99.9
CHC-CoA: 96.4
Isobutyryl-CoA: 80.3
Benzoyl-CoA: 79.7
Acetyl-CoA: 56.2
inactive: 36.1
3-Methylbutyryl-CoA: 33.5
fatty_acid: 25.8

PKS_AT (3295..3571): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.2
inactive: 95.5
Methoxymalonyl-CoA: 70.9
Methylmalonyl-CoA: 53.4
Propionyl-CoA: 43.2
Isobutyryl-CoA: 31.6
Benzoyl-CoA: 28.8
Acetyl-CoA: 25.1
fatty_acid: 21.5
2-Methylbutyryl-CoA: 19.5
Ethylmalonyl-CoA: 14.8
trans-1,2-CPDA: 11.7
CHC-CoA: 9.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (4788..5066): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 195.6
Ethylmalonyl-CoA: 187.5
Methoxymalonyl-CoA: 162.0
2-Methylbutyryl-CoA: 121.3
trans-1,2-CPDA: 116.9
Malonyl-CoA: 110.7
Propionyl-CoA: 108.8
CHC-CoA: 95.8
Benzoyl-CoA: 80.4
Isobutyryl-CoA: 76.6
Acetyl-CoA: 50.0
inactive: 42.1
fatty_acid: 24.7
3-Methylbutyryl-CoA: 24.6

PKS_AT (6414..6690): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.0
inactive: 97.1
Methoxymalonyl-CoA: 71.6
Methylmalonyl-CoA: 55.8
Propionyl-CoA: 42.7
Isobutyryl-CoA: 32.8
Benzoyl-CoA: 29.3
Acetyl-CoA: 25.6
fatty_acid: 20.5
2-Methylbutyryl-CoA: 19.4
Ethylmalonyl-CoA: 16.2
trans-1,2-CPDA: 12.1
CHC-CoA: 10.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (8112..8394): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 195.7
Ethylmalonyl-CoA: 185.2
Methoxymalonyl-CoA: 160.5
2-Methylbutyryl-CoA: 128.9
Propionyl-CoA: 105.2
trans-1,2-CPDA: 102.7
Malonyl-CoA: 101.1
CHC-CoA: 96.6
Isobutyryl-CoA: 80.2
Benzoyl-CoA: 78.9
Acetyl-CoA: 55.6
inactive: 35.6
3-Methylbutyryl-CoA: 33.0
fatty_acid: 26.0

ABI94380.1: mmal - mal - pk - mal

PKS_AT (626..907): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 198.4
Ethylmalonyl-CoA: 192.8
Methoxymalonyl-CoA: 165.6
2-Methylbutyryl-CoA: 135.3
trans-1,2-CPDA: 121.5
Propionyl-CoA: 111.8
Malonyl-CoA: 102.5
CHC-CoA: 97.5
Isobutyryl-CoA: 77.1
Benzoyl-CoA: 71.9
Acetyl-CoA: 53.3
inactive: 37.3
fatty_acid: 27.1
3-Methylbutyryl-CoA: 20.7

PKS_AT (2631..2908): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 209.8
inactive: 96.2
Methoxymalonyl-CoA: 70.1
Methylmalonyl-CoA: 53.5
Propionyl-CoA: 38.4
Isobutyryl-CoA: 31.6
Benzoyl-CoA: 30.7
Acetyl-CoA: 24.9
fatty_acid: 20.9
2-Methylbutyryl-CoA: 16.1
trans-1,2-CPDA: 16.0
Ethylmalonyl-CoA: 12.6
CHC-CoA: 9.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (4647..4940): pk

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 168.4
Ethylmalonyl-CoA: 165.6
Methoxymalonyl-CoA: 140.6
2-Methylbutyryl-CoA: 128.3
Propionyl-CoA: 105.6
trans-1,2-CPDA: 103.5
CHC-CoA: 90.4
Malonyl-CoA: 72.9
Isobutyryl-CoA: 62.8
Benzoyl-CoA: 44.5
fatty_acid: 36.8
Acetyl-CoA: 26.1
3-Methylbutyryl-CoA: 25.8
inactive: 22.5

PKS_AT (6357..6634): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 209.8
inactive: 96.2
Methoxymalonyl-CoA: 70.1
Methylmalonyl-CoA: 53.5
Propionyl-CoA: 38.4
Isobutyryl-CoA: 31.6
Benzoyl-CoA: 30.7
Acetyl-CoA: 24.9
fatty_acid: 20.9
2-Methylbutyryl-CoA: 16.1
trans-1,2-CPDA: 16.0
Ethylmalonyl-CoA: 12.6
CHC-CoA: 9.7
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor