Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000158.1:
Cluster 1Polyketide178527

BGC0000158, tautomycetin biosynthetic gene cluster from Streptomyces griseochromogenes. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: tautomycetin
PubChem ID: 16759609
SMILES string: Copy to clipboard
Molecular formula: C33H50O10
Average molecular mass: 606.753 Da
Molecular activity: Inhibitor
Molecular target: Protein Phosphatase

Class-specific details

Biosynthetic class(es):
Polyketide
Polyketide subclass:
Other (linear)
Polyketide synthase subclass:
Modular type I
Starter unit:
Malonyl-CoA
Thioesterase type:
Type I
Release / cyclization type:
Unknown

Modular polyketide synthases:
ttnA (ABV91286)
Module 0
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Scaffold-modifying domain: None
Module 1
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 2
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 3
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 4
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 5
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None

ttnB (ABV91287)
Module 6
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 7
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 8
AT specificity: Ethylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 9
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None

Gene cluster description

tautomycetin (BGC0000158). Gene Cluster 1. Biosynthetic class = Polyketide. GenBank EU035755, positions 1777-76596. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:Unknown
Contact for this cluster:Xiaohui Yan (The Scripps Research Institute)

Literature references

1. Li W et al. (2009) Characterization of the tautomycetin biosynthetic gene cluster from Streptomyces griseochromogenes provides new insight into dialkylmaleic anhydride biosynthesis. J Nat Prod 72(3):450-9. doi: 10.1021/np8007478.
2. Luo Y et al. (2010) Functional characterization of TtnD and TtnF, unveiling new insights into tautomycetin biosynthesis. J Am Chem Soc 132(19):6663-71. doi: 10.1021/ja9082446.
3. Liu S et al. (2011) SHP2 is a target of the immunosuppressant tautomycetin. Chem Biol 18(1):101-10. doi: 10.1016/j.chembiol.2010.10.015.
4. Yang D et al. (2012) Functional characterization of ttnI completing the tailoring steps for tautomycetin biosynthesis in Streptomyces griseochromogenes. Org Lett 14(5):1302-5. doi: 10.1021/ol300187p. Epub 2012 Feb 17.