BGC0000159 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000159 (Streptomyces spiroverticillatus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	94,050	High	tautomycin	PKS:Type I

BGC0000159 - Region 1 - T1PKS

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Location: 1 - 94,050 nt. (total: 94,050 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 94,051
Label		BGC0000159

Tailoring functions

Oxidoreductases: dehydrogenase

ABW96534.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

ttmA: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

ttmB: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

ttmH: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Hydrolases: esterase phosphatase

ttmE: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

ttmT: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: decarboxylase dehydratase

ttmG: putative carboxymuconolactone decarboxylase

smcogs: SMCOG1298: putative carboxymuconolactone decarboxylase

ttmP: L-carnitine dehydratase/bile acid-inducible

smcogs: SMCOG1189: L-carnitine dehydratase/bile acid-inducible

Ligases: synthase

ttmI: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ttmJ: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000159.5	1.00	PKS	tautomycin	Streptomyces spiroverticillatus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0000067.5	0.66	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001051.5	0.60	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0000068.5	0.60	PKS	geldanamycin	Streptomyces hygroscopicus
BGC0000041.3	0.59	PKS	oronofacic acid	Pseudomonas syringae
BGC0002276.2	0.58	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001012.3	0.58	NRPS, PKS	meridamycin	Streptomyces violaceusniger
BGC0001495.3	0.57	PKS	actinoallolide A	Actinoallomurus fulvus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000159.5	1.00	PKS	tautomycin	Streptomyces spiroverticillatus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0000067.5	0.66	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001051.5	0.60	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0000068.5	0.60	PKS	geldanamycin	Streptomyces hygroscopicus
BGC0000041.3	0.59	PKS	oronofacic acid	Pseudomonas syringae
BGC0002276.2	0.58	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001012.3	0.58	NRPS, PKS	meridamycin	Streptomyces violaceusniger
BGC0001495.3	0.57	PKS	actinoallolide A	Actinoallomurus fulvus

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 94050:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - pk - ohmal - Me-ccmal - ccmal - Me-redmal - Me-ohmal) + (redmal - ohmal - Me-redmal) + (ccmal - Me-redmal - ccmal)
Non-colinear ordering:: ttmJ + ttmI + ttmH

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ttmH: mal - mmal - mal

PKS_AT (570..858): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 214.1
inactive: 92.8
Methoxymalonyl-CoA: 62.8
Methylmalonyl-CoA: 55.0
Isobutyryl-CoA: 50.7
Propionyl-CoA: 37.9
fatty_acid: 23.1
2-Methylbutyryl-CoA: 22.8
Ethylmalonyl-CoA: 22.5
Acetyl-CoA: 20.5
Benzoyl-CoA: 20.3
CHC-CoA: 17.7
trans-1,2-CPDA: 15.9
3-Methylbutyryl-CoA: 0.0

PKS_AT (2282..2576): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.2
Ethylmalonyl-CoA: 184.7
Methoxymalonyl-CoA: 164.9
2-Methylbutyryl-CoA: 138.6
Propionyl-CoA: 114.8
trans-1,2-CPDA: 112.8
Malonyl-CoA: 102.8
CHC-CoA: 99.9
Isobutyryl-CoA: 74.6
Benzoyl-CoA: 71.0
Acetyl-CoA: 53.5
inactive: 40.9
fatty_acid: 34.9
3-Methylbutyryl-CoA: 32.7

PKS_AT (4339..4629): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 66.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.6
inactive: 84.4
Methoxymalonyl-CoA: 66.2
Methylmalonyl-CoA: 51.9
Propionyl-CoA: 35.6
Isobutyryl-CoA: 33.8
Acetyl-CoA: 18.1
Benzoyl-CoA: 17.8
trans-1,2-CPDA: 15.3
fatty_acid: 15.2
CHC-CoA: 11.9
2-Methylbutyryl-CoA: 11.8
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -4.5

ttmI: mal - mal - mmal

PKS_AT (571..861): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 206.7
inactive: 86.7
Methoxymalonyl-CoA: 60.5
Methylmalonyl-CoA: 52.6
Propionyl-CoA: 42.4
Isobutyryl-CoA: 26.6
trans-1,2-CPDA: 23.9
2-Methylbutyryl-CoA: 23.5
Acetyl-CoA: 23.3
Ethylmalonyl-CoA: 20.9
Benzoyl-CoA: 19.8
fatty_acid: 19.5
CHC-CoA: 15.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2610..2898): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.9
inactive: 80.4
Methoxymalonyl-CoA: 64.1
Methylmalonyl-CoA: 51.2
Isobutyryl-CoA: 34.3
Propionyl-CoA: 33.3
Acetyl-CoA: 25.0
Benzoyl-CoA: 23.2
2-Methylbutyryl-CoA: 17.8
fatty_acid: 14.8
Ethylmalonyl-CoA: 13.3
CHC-CoA: 12.4
trans-1,2-CPDA: 12.2
3-Methylbutyryl-CoA: 0.0

PKS_AT (4128..4422): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.0
Ethylmalonyl-CoA: 204.6
Methoxymalonyl-CoA: 170.9
2-Methylbutyryl-CoA: 151.8
Propionyl-CoA: 127.7
trans-1,2-CPDA: 126.6
CHC-CoA: 114.5
Malonyl-CoA: 103.6
Isobutyryl-CoA: 70.6
Benzoyl-CoA: 70.0
Acetyl-CoA: 54.0
fatty_acid: 44.4
inactive: 30.8
3-Methylbutyryl-CoA: 29.4

ttmJ: mmal - pk - mal - mmal - mal - mmal - mmal

PKS_AT (104..400): mmal

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 91.7%; Methylmalonyl-CoA: 79.2%; 2-Methylbutyryl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 163.4
Ethylmalonyl-CoA: 161.4
2-Methylbutyryl-CoA: 150.8
Methoxymalonyl-CoA: 129.0
Propionyl-CoA: 118.4
Malonyl-CoA: 97.9
trans-1,2-CPDA: 97.0
CHC-CoA: 95.5
Isobutyryl-CoA: 59.7
inactive: 39.7
Benzoyl-CoA: 37.2
Acetyl-CoA: 23.8
3-Methylbutyryl-CoA: 21.6
fatty_acid: 17.9

PKS_AT (1093..1393): pk

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 91.7%; Methylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 188.4
Ethylmalonyl-CoA: 187.9
Methoxymalonyl-CoA: 187.7
2-Methylbutyryl-CoA: 127.4
trans-1,2-CPDA: 104.7
CHC-CoA: 104.0
Propionyl-CoA: 101.1
Malonyl-CoA: 99.8
Isobutyryl-CoA: 66.2
Benzoyl-CoA: 51.6
Acetyl-CoA: 50.0
3-Methylbutyryl-CoA: 35.0
fatty_acid: 34.1
inactive: 19.1

PKS_AT (2815..3104): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 225.2
inactive: 91.5
Methoxymalonyl-CoA: 67.8
Methylmalonyl-CoA: 65.7
Propionyl-CoA: 42.2
Isobutyryl-CoA: 30.4
Benzoyl-CoA: 22.0
Ethylmalonyl-CoA: 20.5
Acetyl-CoA: 20.3
2-Methylbutyryl-CoA: 19.2
CHC-CoA: 17.6
trans-1,2-CPDA: 16.2
fatty_acid: 14.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (4330..4622): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.2
Ethylmalonyl-CoA: 187.1
Methoxymalonyl-CoA: 168.3
2-Methylbutyryl-CoA: 153.0
Propionyl-CoA: 123.3
trans-1,2-CPDA: 113.5
CHC-CoA: 103.7
Malonyl-CoA: 98.6
Isobutyryl-CoA: 79.1
Benzoyl-CoA: 58.7
Acetyl-CoA: 55.1
inactive: 40.3
fatty_acid: 32.8
3-Methylbutyryl-CoA: 24.8

PKS_AT (6024..6314): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.6
inactive: 95.0
Methoxymalonyl-CoA: 66.5
Methylmalonyl-CoA: 55.3
Propionyl-CoA: 40.5
Isobutyryl-CoA: 33.5
Ethylmalonyl-CoA: 25.3
fatty_acid: 24.7
2-Methylbutyryl-CoA: 23.8
Acetyl-CoA: 21.1
Benzoyl-CoA: 20.3
CHC-CoA: 16.7
trans-1,2-CPDA: 14.4
3-Methylbutyryl-CoA: 0.0

PKS_AT (7744..8038): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 206.9
Ethylmalonyl-CoA: 200.4
Methoxymalonyl-CoA: 174.1
2-Methylbutyryl-CoA: 148.3
Propionyl-CoA: 123.5
trans-1,2-CPDA: 121.2
CHC-CoA: 113.9
Malonyl-CoA: 95.6
Isobutyryl-CoA: 78.7
Benzoyl-CoA: 76.2
Acetyl-CoA: 60.7
inactive: 38.9
3-Methylbutyryl-CoA: 38.9
fatty_acid: 38.7

PKS_AT (9816..10110): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 206.7
Ethylmalonyl-CoA: 198.2
Methoxymalonyl-CoA: 174.7
2-Methylbutyryl-CoA: 146.5
Propionyl-CoA: 122.1
trans-1,2-CPDA: 120.6
CHC-CoA: 114.8
Malonyl-CoA: 96.2
Isobutyryl-CoA: 77.0
Benzoyl-CoA: 76.2
Acetyl-CoA: 58.7
fatty_acid: 39.1
inactive: 36.5
3-Methylbutyryl-CoA: 36.4

Link to PARAS substrate predictor