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Overview
1.1

### Identified secondary metabolite regions using strictness 'relaxed'

BGC0000162 (Micromonospora chalcea)

Region | Type | From | To | Similarity Confidence | Most similar known cluster
--- | --- | --- | --- | --- | ---
Region 1 | other,T1PKS | 1 | 108,236 | High | tetrocarcin A | PKS

BGC0000162 - Region 1 - T1PKS,other
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
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Location: 1 - 108,236 nt. (total: 108,236 nt) Show pHMM detection rules used
Region on contig edge.
other: (Neocarzinostat or bcpB or frbD or mitE or prnB or CaiA or bacilysin or orf2_PTase)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
MIBiG
Tailoring
MIBiG comparison
KnownClusterBlast
NRPS/PKS modules
NRPS/PKS domains
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
External annotation
This annotation was made externally by 'MIBiG' and not by antiSMASH

Name |  | MIBiG
Version |  | 4.0alpha

Type |  | Subregion
Location |  | 1 - 108,237
Label |  | BGC0000162

Tailoring functions
Tailoring enzymes detected in this region
Oxidoreductases: dehydrogenase oxidase oxidoreductase oxygenase
ACB37724.1: flavin reductase domain protein FMN-binding

smcogs
  SMCOG1160: flavin reductase domain protein FMN-binding

ACB37729.1: oxidoreductase

smcogs
  SMCOG1079: oxidoreductase

ACB37736.1: acyl-CoA dehydrogenase

smcogs
  SMCOG1006: acyl-CoA dehydrogenase

ACB37744.1: monooxygenase FAD-binding

smcogs
  SMCOG1050: monooxygenase FAD-binding

ACB37752.1: FAD linked oxidase domain protein

smcogs
  SMCOG1138: FAD linked oxidase domain protein

ACB37756.1: aldo/keto reductase family oxidoreductase

smcogs
  SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: acylase acyltransferase aminotransferase glycosyltransferase methyltransferase transferase
ACB37723.1: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

ACB37730.1: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

ACB37731.1: methyltransferase

smcogs
  SMCOG1089: methyltransferase

ACB37732.1: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs
  SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

ACB37733.1: acyltransferase 3

smcogs
  SMCOG1088: acyltransferase 3

ACB37734.1: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs
  SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

ACB37737.1: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs
  SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

ACB37750.1: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

ACB37751.1: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

ACB37755.1: malonyl CoA-acyl carrier protein transacylase

smcogs
  SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase phosphatase
ACB37746.1: FkbH like protein

smcogs
  SMCOG1256: FkbH like protein

ACB37753.1: thioesterase

smcogs
  SMCOG1004: thioesterase

Lyases: cyclase dehydratase
ACB37735.1: NAD-dependent epimerase/dehydratase

smcogs
  SMCOG1010: NAD-dependent epimerase/dehydratase

ACB37739.1: Diels-Alderase cyclase

extras
  DA_cyclase: Diels-Alderase cyclase

Ligases: synthase
ACB37740.1: Beta-ketoacyl synthase

smcogs
  SMCOG1093: Beta-ketoacyl synthase

ACB37741.1: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

ACB37742.1: Beta-ketoacyl synthase

smcogs
  SMCOG1093: Beta-ketoacyl synthase

ACB37743.1: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

ACB37745.1: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs
  SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters
Shows areas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | other | T1PKS | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | --- | ---
BGC0000162.5 |  |  | 1.31 | PKS | tetrocarcin A | Micromonospora chalcea
BGC0001817.5 |  |  | 0.59 | other (other) | vancosamine | Streptomyces sp. WAC2288
BGC0001244.3 |  |  | 0.46 | PKS | (-)-Mellein | Parastagonospora nodorum
BGC0001276.3 |  |  | 0.45 | PKS | 6-methylsalicyclic acid | Aspergillus terreus
BGC0001273.3 |  |  | 0.45 | PKS | asperlactone | Aspergillus ochraceus
BGC0000898.5 |  |  | 0.40 | saccharide | desosamine, pikromycin, neomethymycin, narbomycin | Streptomyces venezuelae
BGC0000278.3 |  |  | 0.39 | PKS | urdamycin | Streptomyces fradiae
BGC0000899.4 |  |  | 0.39 | saccharide | desosamine | Streptomyces narbonensis
BGC0001004.5 |  |  | 0.39 | PKS | lobophorin B | Streptomyces sp. SCSIO 01127
BGC0000790.3 |  |  | 0.38 | saccharide | O-antigen | Escherichia coli

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0000162.5 |  | 1.00 | PKS | tetrocarcin A | Micromonospora chalcea
BGC0001244.3 |  | 0.76 | PKS | (-)-Mellein | Parastagonospora nodorum
BGC0001276.3 |  | 0.76 | PKS | 6-methylsalicyclic acid | Aspergillus terreus
BGC0001273.3 |  | 0.75 | PKS | asperlactone | Aspergillus ochraceus
BGC0001257.3 |  | 0.67 | PKS | 1,3,6,8-tetrahydroxynaphthalene | Nodulisporium sp. ATCC74245
BGC0002154.2 |  | 0.66 | PKS | 1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene | Pyricularia oryzae 70-15
BGC0001284.4 |  | 0.66 | PKS | alternariol | Parastagonospora nodorum SN15
BGC0002191.2 |  | 0.66 | PKS | prolipyrone B, gibepyrone D | Fusarium graminearum PH-1
BGC0000041.3 |  | 0.65 | PKS | oronofacic acid | Pseudomonas syringae
BGC0001254.3 |  | 0.64 | PKS | ACT-Toxin II | Alternaria alternata

Similar known clusters from MIBiG 4.0
Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.
Click on an accession to open that entry in the MIBiG database.
Location:
Identifier
Identity
Coverage
Module view
Shows module structures for each candidate cluster in NRPS and PKS regions.
Genes are shown in predicted order, and are only present when containing at least one complete module.
A domain glossary is available here, and an explanation of the visualisation is available here.
Legend
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains
NRPS/PKS products
NRPS/PKS substrates
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 1, location 1289 - 108236:
Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:
  (pk - pk - pk - Me-ccmal) + (ccmal - Me-ccmal - ccmal - ccmal) + (Me-ccmal) + (pk - Me-ccmal) + (ohmal)
Non-colinear ordering:
  ACB37740.1 + ACB37741.1 + ACB37742.1 + ACB37755.1 + ACB37743.1

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 5662 - 108236:
Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:
  (pk - pk - pk - Me-ccmal) + (ccmal - Me-ccmal - ccmal - ccmal) + (Me-ccmal) + (pk - Me-ccmal) + (ohmal)
Non-colinear ordering:
  ACB37740.1 + ACB37741.1 + ACB37742.1 + ACB37755.1 + ACB37743.1

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).
ACB37740.1: pk - pk - pk - mmal
PKS_AT (540..834): pk
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 100.0%
  Methylmalonyl-CoA: 83.3%
  Methoxymalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 177.1
  Ethylmalonyl-CoA: 166.9
  Methoxymalonyl-CoA: 137.3
  trans-1,2-CPDA: 95.8
  Malonyl-CoA: 93.2
  CHC-CoA: 87.9
  2-Methylbutyryl-CoA: 82.6
  Propionyl-CoA: 75.6
  Isobutyryl-CoA: 54.9
  Benzoyl-CoA: 47.0
  Acetyl-CoA: 32.7
  fatty_acid: 31.7
  inactive: 27.8
  3-Methylbutyryl-CoA: 21.9

PKS_AT (1551..1849): pk
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 100.0%
  Methylmalonyl-CoA: 95.8%
  Methoxymalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 193.4
  Ethylmalonyl-CoA: 187.7
  Methoxymalonyl-CoA: 159.8
  2-Methylbutyryl-CoA: 125.4
  trans-1,2-CPDA: 108.2
  Propionyl-CoA: 103.4
  Malonyl-CoA: 83.8
  CHC-CoA: 79.6
  Isobutyryl-CoA: 68.7
  Benzoyl-CoA: 66.7
  Acetyl-CoA: 40.9
  3-Methylbutyryl-CoA: 32.6
  inactive: 29.7
  fatty_acid: 29.6

PKS_AT (3309..3600): pk
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 100.0%
  Methylmalonyl-CoA: 83.3%
  Methoxymalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 177.1
  Ethylmalonyl-CoA: 166.9
  Methoxymalonyl-CoA: 137.3
  trans-1,2-CPDA: 95.8
  Malonyl-CoA: 93.2
  CHC-CoA: 87.9
  2-Methylbutyryl-CoA: 82.6
  Propionyl-CoA: 75.6
  Isobutyryl-CoA: 54.9
  Benzoyl-CoA: 47.0
  Acetyl-CoA: 32.7
  fatty_acid: 31.7
  inactive: 27.8
  3-Methylbutyryl-CoA: 21.9

PKS_AT (5048..5342): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Malonyl-CoA: 83.3%
  Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 180.0
  Ethylmalonyl-CoA: 175.3
  Methoxymalonyl-CoA: 150.4
  trans-1,2-CPDA: 119.4
  2-Methylbutyryl-CoA: 105.5
  Malonyl-CoA: 101.7
  CHC-CoA: 95.8
  Propionyl-CoA: 86.7
  Isobutyryl-CoA: 47.9
  Benzoyl-CoA: 46.5
  inactive: 38.5
  Acetyl-CoA: 25.5
  fatty_acid: 20.4
  3-Methylbutyryl-CoA: 7.7

ACB37741.1: mal - mmal - mal - mal
PKS_AT (564..851): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 100.0%
  inactive: 75.0%
  Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 212.2
  inactive: 101.1
  Methoxymalonyl-CoA: 67.9
  Methylmalonyl-CoA: 64.3
  Propionyl-CoA: 36.7
  Ethylmalonyl-CoA: 32.2
  Isobutyryl-CoA: 29.7
  Acetyl-CoA: 19.8
  2-Methylbutyryl-CoA: 19.7
  Benzoyl-CoA: 13.7
  fatty_acid: 13.5
  3-Methylbutyryl-CoA: 10.1
  CHC-CoA: 9.5
  trans-1,2-CPDA: 7.8

PKS_AT (2265..2556): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Malonyl-CoA: 83.3%
  Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 178.0
  Ethylmalonyl-CoA: 172.6
  Methoxymalonyl-CoA: 150.3
  trans-1,2-CPDA: 119.5
  2-Methylbutyryl-CoA: 102.5
  Malonyl-CoA: 101.0
  CHC-CoA: 95.9
  Propionyl-CoA: 87.1
  Isobutyryl-CoA: 48.1
  Benzoyl-CoA: 42.7
  inactive: 37.5
  Acetyl-CoA: 25.5
  fatty_acid: 20.4
  3-Methylbutyryl-CoA: 8.1

PKS_AT (4012..4293): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 100.0%
  inactive: 66.7%
  Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 202.9
  inactive: 83.9
  Methoxymalonyl-CoA: 66.5
  Methylmalonyl-CoA: 55.0
  Isobutyryl-CoA: 32.9
  Propionyl-CoA: 23.4
  Ethylmalonyl-CoA: 20.3
  fatty_acid: 20.2
  Acetyl-CoA: 15.3
  Benzoyl-CoA: 14.8
  2-Methylbutyryl-CoA: 9.5
  trans-1,2-CPDA: 0.0
  CHC-CoA: 0.0
  3-Methylbutyryl-CoA: 0.0

PKS_AT (5754..6050): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 100.0%
  inactive: 79.2%
  Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 177.3
  inactive: 87.0
  Methoxymalonyl-CoA: 65.1
  Methylmalonyl-CoA: 54.8
  Isobutyryl-CoA: 44.2
  Ethylmalonyl-CoA: 23.4
  Benzoyl-CoA: 20.7
  Propionyl-CoA: 19.3
  Acetyl-CoA: 15.0
  2-Methylbutyryl-CoA: 13.2
  fatty_acid: 12.8
  CHC-CoA: 9.8
  trans-1,2-CPDA: 0.0
  3-Methylbutyryl-CoA: 0.0

ACB37742.1: mmal
PKS_AT (562..856): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Malonyl-CoA: 83.3%
  Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 177.1
  Ethylmalonyl-CoA: 166.5
  Methoxymalonyl-CoA: 145.4
  trans-1,2-CPDA: 113.9
  Malonyl-CoA: 99.4
  2-Methylbutyryl-CoA: 99.1
  CHC-CoA: 98.3
  Propionyl-CoA: 85.1
  Isobutyryl-CoA: 46.8
  Benzoyl-CoA: 37.1
  inactive: 35.5
  Acetyl-CoA: 24.1
  fatty_acid: 19.1
  3-Methylbutyryl-CoA: 6.5

ACB37743.1: mal
PKS_AT (571..867): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 100.0%
  inactive: 79.2%
  Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 194.5
  inactive: 92.7
  Methoxymalonyl-CoA: 60.0
  Methylmalonyl-CoA: 51.1
  Propionyl-CoA: 40.1
  Ethylmalonyl-CoA: 19.8
  2-Methylbutyryl-CoA: 16.9
  Acetyl-CoA: 14.8
  Isobutyryl-CoA: 10.8
  fatty_acid: 7.9
  trans-1,2-CPDA: 0.0
  CHC-CoA: 0.0
  Benzoyl-CoA: 0.0
  3-Methylbutyryl-CoA: 0.0

ACB37755.1: pk - mmal
PKS_AT (582..880): pk
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:
  Ethylmalonyl-CoA: 215.7
  Methylmalonyl-CoA: 208.4
  Methoxymalonyl-CoA: 172.0
  trans-1,2-CPDA: 141.0
  2-Methylbutyryl-CoA: 137.9
  Propionyl-CoA: 135.7
  Malonyl-CoA: 109.3
  CHC-CoA: 104.4
  Isobutyryl-CoA: 75.8
  Benzoyl-CoA: 60.7
  fatty_acid: 53.9
  Acetyl-CoA: 45.3
  3-Methylbutyryl-CoA: 34.4
  inactive: 31.7

PKS_AT (2675..2971): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 218.6
  Ethylmalonyl-CoA: 209.0
  Methoxymalonyl-CoA: 185.0
  2-Methylbutyryl-CoA: 155.2
  trans-1,2-CPDA: 141.6
  Propionyl-CoA: 138.4
  CHC-CoA: 120.8
  Malonyl-CoA: 113.7
  Isobutyryl-CoA: 108.3
  Benzoyl-CoA: 71.4
  Acetyl-CoA: 64.3
  3-Methylbutyryl-CoA: 59.8
  fatty_acid: 46.2
  inactive: 32.5

Link to PARAS substrate predictor