BGC0000163 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000163 (Streptomyces longisporoflavus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	169,690	High	tetronasin	PKS

BGC0000163 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 169,690 nt. (total: 169,690 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 169,691
Label		BGC0000163

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

ACR50754.1: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

ACR50761.1: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

tsn11: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

tsn12: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

tsn18: dioxygenase, TauD/TfdA

smcogs: SMCOG1121: dioxygenase, TauD/TfdA

tsn20: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

tsnC: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

Transferases: kinase methyltransferase

ACR50753.1: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

tsn25: methyltransferase

smcogs: SMCOG1089: methyltransferase

tsn8: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: esterase

tsn20a: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

tsn13: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

tsn19: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

tsn21: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

tsn3: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

tsn9: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

tsnAI: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

tsnAII: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tsnAIII: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tsnAIV: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

tsnAV: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tsnDI: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tsnDII: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

tsnDIII: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Translocases: transporter

ACR50757.1: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000163.5	0.98	PKS	tetronasin	Streptomyces longisporoflavus
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001284.4	0.67	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001254.3	0.67	PKS	ACT-Toxin II	Alternaria alternata
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000163.5	1.00	PKS	tetronasin	Streptomyces longisporoflavus
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001284.4	0.67	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001254.3	0.67	PKS	ACT-Toxin II	Alternaria alternata
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 6100 - 156011:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - mal - ohmal) + (Me-ccmal - ccmal - ohmal) + (Me-redmal - Me-ccmal - ccmal) + (redmal - Me-redmal - Me-ccmal) + (mal - ccmal - Me-redmal) + (ohmal) + (redmal)
Non-colinear ordering:: tsnDI + tsnAII + tsnAIII + tsnAIV + tsnAI + tsnAV + tsnDIII

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

tsnAII: mmal - mal - mal

PKS_AT (573..871): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 195.3
Ethylmalonyl-CoA: 190.3
Methoxymalonyl-CoA: 161.6
2-Methylbutyryl-CoA: 135.3
CHC-CoA: 122.5
trans-1,2-CPDA: 106.6
Malonyl-CoA: 98.5
Propionyl-CoA: 96.3
Isobutyryl-CoA: 47.1
Benzoyl-CoA: 44.7
inactive: 28.9
fatty_acid: 21.5
Acetyl-CoA: 19.7
3-Methylbutyryl-CoA: 19.2

PKS_AT (2301..2595): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 145.4
inactive: 73.0
Methoxymalonyl-CoA: 46.1
Methylmalonyl-CoA: 30.7
Propionyl-CoA: 18.8
Isobutyryl-CoA: 15.0
fatty_acid: 13.7
Acetyl-CoA: 12.6
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (4050..4343): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 145.2
inactive: 74.8
Methoxymalonyl-CoA: 48.1
Methylmalonyl-CoA: 32.8
Propionyl-CoA: 22.1
Isobutyryl-CoA: 19.4
Acetyl-CoA: 14.4
fatty_acid: 13.4
trans-1,2-CPDA: 9.2
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.0

tsnAIII: mmal - mmal - mal

PKS_AT (570..868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 195.3
Ethylmalonyl-CoA: 190.3
Methoxymalonyl-CoA: 161.6
2-Methylbutyryl-CoA: 135.3
CHC-CoA: 122.5
trans-1,2-CPDA: 106.6
Malonyl-CoA: 98.5
Propionyl-CoA: 96.3
Isobutyryl-CoA: 47.1
Benzoyl-CoA: 44.7
inactive: 28.9
fatty_acid: 21.5
Acetyl-CoA: 19.7
3-Methylbutyryl-CoA: 19.2

PKS_AT (2630..2933): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 193.9
Ethylmalonyl-CoA: 188.7
Methoxymalonyl-CoA: 160.2
2-Methylbutyryl-CoA: 133.1
CHC-CoA: 124.6
trans-1,2-CPDA: 105.8
Propionyl-CoA: 98.9
Malonyl-CoA: 98.9
Isobutyryl-CoA: 49.4
Benzoyl-CoA: 44.5
inactive: 28.9
fatty_acid: 23.0
Acetyl-CoA: 22.0
3-Methylbutyryl-CoA: 20.9

PKS_AT (4369..4662): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 150.7
inactive: 76.2
Methoxymalonyl-CoA: 50.2
Methylmalonyl-CoA: 33.5
Propionyl-CoA: 20.4
Isobutyryl-CoA: 19.1
fatty_acid: 13.8
Acetyl-CoA: 12.6
trans-1,2-CPDA: 8.2
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.0

tsnAIV: mal - mmal - mmal

PKS_AT (569..863): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 150.5
inactive: 77.0
Methoxymalonyl-CoA: 49.2
Methylmalonyl-CoA: 33.9
Propionyl-CoA: 19.8
Isobutyryl-CoA: 19.1
fatty_acid: 13.8
Acetyl-CoA: 12.6
trans-1,2-CPDA: 8.2
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.0

PKS_AT (2676..2979): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.3
Ethylmalonyl-CoA: 184.3
Methoxymalonyl-CoA: 156.8
2-Methylbutyryl-CoA: 126.8
CHC-CoA: 121.4
trans-1,2-CPDA: 109.3
Propionyl-CoA: 92.8
Malonyl-CoA: 92.7
Isobutyryl-CoA: 45.9
Benzoyl-CoA: 44.0
inactive: 28.7
Acetyl-CoA: 21.3
fatty_acid: 19.1
3-Methylbutyryl-CoA: 18.5

PKS_AT (4755..5058): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 192.7
Ethylmalonyl-CoA: 187.3
Methoxymalonyl-CoA: 159.9
2-Methylbutyryl-CoA: 129.5
CHC-CoA: 121.7
trans-1,2-CPDA: 105.4
Propionyl-CoA: 95.7
Malonyl-CoA: 93.3
Isobutyryl-CoA: 46.2
Benzoyl-CoA: 44.2
inactive: 28.7
fatty_acid: 20.7
Acetyl-CoA: 20.0
3-Methylbutyryl-CoA: 18.1

tsnAV: mal

PKS_AT (563..864): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 191.8
inactive: 95.1
Methoxymalonyl-CoA: 68.6
Methylmalonyl-CoA: 47.9
Acetyl-CoA: 25.6
Isobutyryl-CoA: 24.8
Propionyl-CoA: 19.3
Ethylmalonyl-CoA: 17.3
CHC-CoA: 13.8
Benzoyl-CoA: 10.1
2-Methylbutyryl-CoA: 7.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

tsnAI: mal - mal - mmal

PKS_AT (581..881): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 223.0
inactive: 117.2
Methoxymalonyl-CoA: 70.9
Methylmalonyl-CoA: 55.9
Propionyl-CoA: 40.4
Isobutyryl-CoA: 31.8
Ethylmalonyl-CoA: 24.6
2-Methylbutyryl-CoA: 19.6
Benzoyl-CoA: 18.5
Acetyl-CoA: 18.4
CHC-CoA: 10.2
fatty_acid: 9.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (1623..1916): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 148.5
inactive: 73.5
Methoxymalonyl-CoA: 48.3
Methylmalonyl-CoA: 35.2
Propionyl-CoA: 21.7
Isobutyryl-CoA: 19.6
fatty_acid: 14.0
Acetyl-CoA: 13.5
trans-1,2-CPDA: 8.5
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.0

PKS_AT (3401..3704): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 193.9
Ethylmalonyl-CoA: 187.6
Methoxymalonyl-CoA: 158.7
2-Methylbutyryl-CoA: 133.2
CHC-CoA: 122.4
trans-1,2-CPDA: 111.2
Malonyl-CoA: 98.4
Propionyl-CoA: 93.6
Isobutyryl-CoA: 46.9
Benzoyl-CoA: 44.6
inactive: 29.1
Acetyl-CoA: 20.8
3-Methylbutyryl-CoA: 19.6
fatty_acid: 19.5

tsnDIII: mal

PKS_AT (534..827): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 66.7%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 142.6
inactive: 70.4
Methylmalonyl-CoA: 55.5
Methoxymalonyl-CoA: 54.1
Propionyl-CoA: 48.5
Isobutyryl-CoA: 44.1
Ethylmalonyl-CoA: 31.0
CHC-CoA: 28.5
2-Methylbutyryl-CoA: 25.5
Benzoyl-CoA: 19.1
Acetyl-CoA: 11.0
trans-1,2-CPDA: 9.7
fatty_acid: 9.0
3-Methylbutyryl-CoA: 0.0

tsn19: X

AMP-binding (21..426): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IGWLVGIASK
Nearest Stachelhaus code(s):: Ile or Val DGLFVGIAVK (35% 8Å match)
Stachelhaus code match:: 60% (weak)

tsnDI: mal

PKS_AT (15..269): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 109.7
inactive: 34.9
Methylmalonyl-CoA: 30.4
Methoxymalonyl-CoA: 27.5
fatty_acid: 27.3
Isobutyryl-CoA: 17.0
trans-1,2-CPDA: 0.0
Propionyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

tsn21: NH2

CAL_domain (181..498): NH2

minowa: NH2

Prediction, score:

NH2: 66.8
Acetyl-CoA: 20.3
fatty_acid: 19.4
shikimic_acid: 0.0
AHBA: 0.0

Link to PARAS substrate predictor