BGC0000165 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000165 (Dactylosporangium aurantiacum subsp. hamdenensis)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,PKS-like	1	82,484	tiacumicin B	PKS:Type I	100%

BGC0000165 - Region 1 - PKS-like,T1PKS

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Location: 1 - 82,484 nt. (total: 82,484 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
PKS-like: (t2pks2 or ksIII)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 82,485
Label		BGC0000165

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000165	1.56	Polyketide	tiacumicin B	Dactylosporangium aurantiacum subsp. hamdenensis
BGC0002084	0.96	RiPP	biarylitide YYH, biarylitide YFH	Planomonospora sp. ID82291
BGC0002099	0.96	RiPP	biarylite-YFH	Planomonospora sp. ID107089
BGC0001273	0.92	Polyketide	asperlactone	Aspergillus ochraceus
BGC0000016	0.91	Other	amphotericin B	Streptomyces nodosus
BGC0001275	0.91	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244	0.91	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0000120	0.87	Polyketide	patulin	Penicillium expansum
BGC0002229	0.87	Polyketide	6-MSA, aculin A	Aspergillus aculeatus ATCC 16872
BGC0002615	0.87	Terpene	4-oxomacrophorin A, macrophorin A, 5'-epimacrophorin B	Penicillium terrestre

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000165	1.00	Polyketide	tiacumicin B	Dactylosporangium aurantiacum subsp. hamdenensis
BGC0000041	0.72	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000844	0.69	Other	clavulanic acid	Streptomyces clavuligerus
BGC0001276	0.67	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275	0.67	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002341	0.67	Other	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001273	0.67	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001244	0.67	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0002608	0.66	NRP, Polyketide	ochratoxin A	Aspergillus steynii
BGC0000160	0.66	Polyketide	terreic acid	Aspergillus terreus NIH2624

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 82484:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - ohmal - Me-ccmal - Me-ccmal) + (pk - Me-ccmal) + (pk) + (ohmal) + (ccmal - Me-ccmal)
Non-colinear ordering:: tiaA1 + tiaA2 + tiaB + tiaA3 + tiaA4

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 34306:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 15447 - 82484:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - ohmal - Me-ccmal - Me-ccmal) + (pk - Me-ccmal) + (ohmal) + (ccmal - Me-ccmal)
Non-colinear ordering:: tiaA1 + tiaA2 + tiaA3 + tiaA4

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

tiaB

PKS_AT (542..827): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Propionyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 117.8
Ethylmalonyl-CoA: 94.2
Methoxymalonyl-CoA: 91.5
Isobutyryl-CoA: 72.8
Malonyl-CoA: 67.1
Propionyl-CoA: 53.6
fatty_acid: 48.2
trans-1,2-CPDA: 41.7
3-Methylbutyryl-CoA: 40.1
2-Methylbutyryl-CoA: 37.9
Benzoyl-CoA: 36.8
Acetyl-CoA: 35.0
CHC-CoA: 29.6
inactive: 17.4

tiaA1

PKS_AT (99..396): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; Propionyl-CoA: 58.3%; Methylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 129.5
Ethylmalonyl-CoA: 121.6
2-Methylbutyryl-CoA: 115.5
Methoxymalonyl-CoA: 112.5
Propionyl-CoA: 95.4
CHC-CoA: 84.3
Malonyl-CoA: 78.7
Isobutyryl-CoA: 68.1
trans-1,2-CPDA: 62.3
Acetyl-CoA: 54.4
Benzoyl-CoA: 38.2
inactive: 36.7
fatty_acid: 9.7
3-Methylbutyryl-CoA: 9.1

PKS_AT (1069..1332): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 165.2
Methoxymalonyl-CoA: 66.2
inactive: 65.0
Methylmalonyl-CoA: 47.7
Isobutyryl-CoA: 40.9
fatty_acid: 33.4
Acetyl-CoA: 31.4
Propionyl-CoA: 28.3
Ethylmalonyl-CoA: 20.9
Benzoyl-CoA: 17.4
CHC-CoA: 16.9
3-Methylbutyryl-CoA: 11.8
2-Methylbutyryl-CoA: 11.3
trans-1,2-CPDA: 7.9

PKS_AT (2502..2779): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 169.2
Ethylmalonyl-CoA: 139.6
Methoxymalonyl-CoA: 129.6
2-Methylbutyryl-CoA: 74.1
trans-1,2-CPDA: 73.4
Propionyl-CoA: 72.3
Malonyl-CoA: 69.2
CHC-CoA: 56.2
Isobutyryl-CoA: 48.5
Benzoyl-CoA: 34.4
inactive: 24.6
Acetyl-CoA: 23.9
fatty_acid: 17.3
3-Methylbutyryl-CoA: 15.2

PKS_AT (4087..4364): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 169.0
Ethylmalonyl-CoA: 135.9
Methoxymalonyl-CoA: 131.1
2-Methylbutyryl-CoA: 84.2
Propionyl-CoA: 77.1
Malonyl-CoA: 74.7
trans-1,2-CPDA: 69.7
CHC-CoA: 59.0
Isobutyryl-CoA: 54.2
Benzoyl-CoA: 39.3
Acetyl-CoA: 34.6
fatty_acid: 28.8
inactive: 23.7
3-Methylbutyryl-CoA: 21.9

tiaA2

PKS_AT (575..873): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 174.5
Methylmalonyl-CoA: 174.4
Methoxymalonyl-CoA: 145.0
2-Methylbutyryl-CoA: 130.9
CHC-CoA: 106.8
Propionyl-CoA: 105.8
trans-1,2-CPDA: 101.8
Malonyl-CoA: 90.2
Isobutyryl-CoA: 81.0
Benzoyl-CoA: 55.9
fatty_acid: 46.8
Acetyl-CoA: 44.5
3-Methylbutyryl-CoA: 27.5
inactive: 11.2

PKS_AT (2072..2349): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 167.8
Ethylmalonyl-CoA: 141.6
Methoxymalonyl-CoA: 128.9
Propionyl-CoA: 74.7
2-Methylbutyryl-CoA: 73.6
trans-1,2-CPDA: 68.6
Malonyl-CoA: 67.0
CHC-CoA: 59.7
Isobutyryl-CoA: 45.4
Benzoyl-CoA: 31.1
Acetyl-CoA: 29.5
inactive: 28.2
fatty_acid: 24.0
3-Methylbutyryl-CoA: 12.1

tiaA3

PKS_AT (545..809): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 172.3
Methoxymalonyl-CoA: 72.8
inactive: 68.5
Methylmalonyl-CoA: 55.3
Isobutyryl-CoA: 48.0
fatty_acid: 44.6
Propionyl-CoA: 29.5
Acetyl-CoA: 26.6
Ethylmalonyl-CoA: 25.6
CHC-CoA: 22.3
Benzoyl-CoA: 19.4
2-Methylbutyryl-CoA: 17.1
trans-1,2-CPDA: 11.5
3-Methylbutyryl-CoA: 10.2

tiaA4

PKS_AT (544..808): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 170.2
Methoxymalonyl-CoA: 70.6
inactive: 68.8
Methylmalonyl-CoA: 53.6
Isobutyryl-CoA: 47.3
fatty_acid: 39.6
Propionyl-CoA: 30.2
Acetyl-CoA: 27.1
Ethylmalonyl-CoA: 22.7
CHC-CoA: 22.2
Benzoyl-CoA: 18.9
2-Methylbutyryl-CoA: 17.0
trans-1,2-CPDA: 11.1
3-Methylbutyryl-CoA: 10.5

PKS_AT (2130..2407): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 165.9
Ethylmalonyl-CoA: 130.4
Methoxymalonyl-CoA: 127.7
2-Methylbutyryl-CoA: 71.1
Propionyl-CoA: 68.5
trans-1,2-CPDA: 67.3
Malonyl-CoA: 66.8
CHC-CoA: 54.0
Isobutyryl-CoA: 47.9
Benzoyl-CoA: 33.4
fatty_acid: 26.9
Acetyl-CoA: 25.8
inactive: 22.4
3-Methylbutyryl-CoA: 16.6