BGC0000167 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000167 (Streptomyces halstedii)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	64,492	vicenistatin	PKS	100%

BGC0000167 - Region 1 - T1PKS

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Location: 1 - 64,492 nt. (total: 64,492 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 64,493
Label		BGC0000167

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000167	1.00	Polyketide	vicenistatin	Streptomyces halstedii
BGC0002634	0.58	Polyketide, NRP, Other	vicenistatin	Streptomyces parvus
BGC0001164	0.32	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001163	0.32	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000041	0.32	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000122	0.31	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0002729	0.31	Polyketide	sordarial	Neurospora crassa OR74A
BGC0001647	0.31	Polyketide	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18
BGC0000278	0.31	Polyketide	urdamycin	Streptomyces fradiae
BGC0001817	0.31	Other	vancosamine	Streptomyces sp. WAC2288

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000167	1.00	Polyketide	vicenistatin	Streptomyces halstedii
BGC0002634	0.83	Polyketide, NRP, Other	vicenistatin	Streptomyces parvus
BGC0001164	0.66	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001163	0.66	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000041	0.65	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000122	0.65	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0002729	0.65	Polyketide	sordarial	Neurospora crassa OR74A
BGC0001647	0.65	Polyketide	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18
BGC0000278	0.64	Polyketide	urdamycin	Streptomyces fradiae
BGC0001817	0.64	Other	vancosamine	Streptomyces sp. WAC2288

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 64492:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (redmal - ccmal - Me-ccmal) + (Me-redmal) + (ccmal - Me-ohmal) + (ccmal - ccmal)
Non-colinear ordering:: vinP1 + vinP2 + vinP3 + vinP4

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

vinP2

PKS_AT (594..906): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 193.7
Ethylmalonyl-CoA: 169.7
Methoxymalonyl-CoA: 138.0
2-Methylbutyryl-CoA: 108.8
trans-1,2-CPDA: 102.0
Malonyl-CoA: 79.7
Propionyl-CoA: 75.0
CHC-CoA: 68.1
Benzoyl-CoA: 61.0
Isobutyryl-CoA: 57.1
Acetyl-CoA: 40.8
inactive: 32.0
3-Methylbutyryl-CoA: 25.1
fatty_acid: 20.9

vinP3

PKS_AT (565..861): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.2
inactive: 104.5
Methoxymalonyl-CoA: 46.8
Methylmalonyl-CoA: 46.5
Propionyl-CoA: 26.4
Acetyl-CoA: 20.9
Isobutyryl-CoA: 16.5
Benzoyl-CoA: 15.5
CHC-CoA: 10.7
2-Methylbutyryl-CoA: 8.8
fatty_acid: 8.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.0

PKS_AT (2274..2582): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 195.6
Ethylmalonyl-CoA: 173.2
Methoxymalonyl-CoA: 139.6
2-Methylbutyryl-CoA: 111.8
trans-1,2-CPDA: 101.3
Malonyl-CoA: 83.5
Propionyl-CoA: 76.3
CHC-CoA: 70.1
Benzoyl-CoA: 63.5
Isobutyryl-CoA: 61.2
Acetyl-CoA: 43.2
inactive: 34.4
3-Methylbutyryl-CoA: 26.8
fatty_acid: 20.9

vinP4

PKS_AT (569..866): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.4
inactive: 107.1
Methoxymalonyl-CoA: 49.1
Methylmalonyl-CoA: 46.8
Propionyl-CoA: 27.0
Acetyl-CoA: 20.9
Isobutyryl-CoA: 16.2
Benzoyl-CoA: 15.5
CHC-CoA: 13.0
Ethylmalonyl-CoA: 12.5
fatty_acid: 8.5
2-Methylbutyryl-CoA: 8.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2295..2591): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.3
inactive: 105.7
Methoxymalonyl-CoA: 48.6
Methylmalonyl-CoA: 47.5
Propionyl-CoA: 27.3
Acetyl-CoA: 20.3
CHC-CoA: 17.0
Isobutyryl-CoA: 15.8
Ethylmalonyl-CoA: 15.7
Benzoyl-CoA: 15.3
fatty_acid: 8.7
2-Methylbutyryl-CoA: 7.4
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

vinK

PKS_AT (40..301): pk

ATSignature: (unknown)

No matches above 50%

minowa: trans-1,2-CPDA

Prediction, score:

trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

vinM

AMP-binding (15..428): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DCFFFALVTK
Nearest Stachelhaus code(s):: bMe-Asp DCFFFALITK (85% 8Å match); Ala DCFFFALITK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

vinN

AMP-binding (5..386): X

nrpys: Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DFYPTSMRIK
Nearest Stachelhaus code(s):: 3S-Abu DYYPTTQRIK (68% 8Å match); Asp DYYPTTMRVK (65% 8Å match)
Stachelhaus code match:: 70% (weak)

vinP1

PKS_AT (668..968): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.6
inactive: 104.5
Methoxymalonyl-CoA: 48.0
Methylmalonyl-CoA: 43.6
Propionyl-CoA: 26.8
Benzoyl-CoA: 17.1
CHC-CoA: 16.1
Ethylmalonyl-CoA: 13.4
Isobutyryl-CoA: 13.3
trans-1,2-CPDA: 11.6
Acetyl-CoA: 11.2
2-Methylbutyryl-CoA: 9.1
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2749..3042): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 201.9
inactive: 97.9
Methoxymalonyl-CoA: 45.2
Methylmalonyl-CoA: 44.4
Propionyl-CoA: 26.6
Benzoyl-CoA: 18.1
Acetyl-CoA: 15.2
CHC-CoA: 14.7
Ethylmalonyl-CoA: 14.5
Isobutyryl-CoA: 11.8
2-Methylbutyryl-CoA: 8.8
fatty_acid: 8.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (4500..4812): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 195.6
Ethylmalonyl-CoA: 173.2
Methoxymalonyl-CoA: 139.6
2-Methylbutyryl-CoA: 111.8
trans-1,2-CPDA: 101.3
Malonyl-CoA: 83.5
Propionyl-CoA: 76.3
CHC-CoA: 70.1
Benzoyl-CoA: 63.5
Isobutyryl-CoA: 61.2
Acetyl-CoA: 43.2
inactive: 34.4
3-Methylbutyryl-CoA: 26.8
fatty_acid: 20.9