BGC0000168 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000168 (Penicillium aethiopicum)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,indole,terpene-precursor	1	51,400	High	viridicatumtoxin	PKS:Iterative type I

BGC0000168 - Region 1 - T1PKS,indole,terpene-precursor

Download region GenBank file

Download region SVG

Location: 1 - 51,400 nt. (total: 51,400 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
indole: (indsynth or dmat or indole_PTase)
terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

Terpene

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 51,401
Label		BGC0000168

Tailoring functions

Oxidoreductases: oxygenase

ADI24930.1: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

ADI24937.1: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Hydrolases: hydrolase

ADI24932.1: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

Ligases: ligase synthase synthetase

ADI24926.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ADI24927.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ADI24929.1: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000168.5	2.10	PKS	viridicatumtoxin	Penicillium aethiopicum
BGC0000013.3	0.88	PKS	alternariol	Aspergillus nidulans FGSC A4
BGC0001144.4	0.70	PKS	neosartoricin B	Trichophyton tonsurans CBS 112818
BGC0001284.4	0.70	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002275.2	0.67	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0002171.2	0.67	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002162.3	0.63	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0001258.3	0.62	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001257.3	0.62	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.62	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000168.5	1.00	PKS	viridicatumtoxin	Penicillium aethiopicum
BGC0000013.3	0.71	PKS	alternariol	Aspergillus nidulans FGSC A4
BGC0001284.4	0.68	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001258.3	0.65	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001257.3	0.65	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.64	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002175.3	0.64	PKS	YWA1	Aspergillus oryzae RIB40
BGC0000107.3	0.64	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum

Similar known clusters from MIBiG 4.0

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

ADI24929.1: PT_FPPS_like

Domain 1 (46 - 349): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, fungal

Reactions:: IPP, DMAPP → FPP

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Terpene

Predicted core structure(s)

For candidate cluster 1, location 531 - 46109:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ADI24926.1: mal

PKS_AT (937..1235): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 62.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 87.6
Methoxymalonyl-CoA: 63.4
Methylmalonyl-CoA: 62.5
inactive: 44.1
Isobutyryl-CoA: 29.4
Propionyl-CoA: 28.2
Benzoyl-CoA: 26.5
2-Methylbutyryl-CoA: 24.6
3-Methylbutyryl-CoA: 22.1
Ethylmalonyl-CoA: 18.3
fatty_acid: 15.9
Acetyl-CoA: 14.9
CHC-CoA: 14.8
trans-1,2-CPDA: 13.5

ADI24927.1: X

AMP-binding (153..592): X

nrpys: (unknown)

SVM prediction details:

NOTE: uncertain match

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: MMWGYT-VNK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate