BGC0000176: difficidin biosynthetic gene cluster from Bacillus velezensis FZB42
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Location: 1 - 69,641 nt. (total: 69,641 nt).
This entry is originally from NCBI GenBank AJ634062.2.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000176
Short description difficidin biosynthetic gene cluster from Bacillus velezensis FZB42
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • PKS (Unknown)
Loci
AJ634062.2
via Knock-out studies
Compounds
  • difficidin
Species Bacillus velezensis FZB42 [taxonomy]
References
Chemical products information
difficidin Evidence:
Copy SMILES
C31H45O6P1
Chemical database entries
NPAtlas
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • CAG23974.1
  • difA
 1 - 2376 (+) malonyl CoA [acyl-carrier-protein] transacylase
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    • CAG23975.2
    • difB
    		 2345 - 3397 (+) putative long-chain fatty acid CoA ligase
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      • CAJ57406.1
      • difC
      		 3422 - 3694 (+) probable acyl carrier protein
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        • CAJ57407.1
        • difD
        		 3691 - 5055 (+) acyl CoA synthetase (AMP forming)/AMP acid ligase II
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          • CAG23976.1
          • difE
          		 5070 - 5807 (+) 3-oxoacyl-(acyl carrier protein) reductase
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            • CAG23977.1
            • difF
            		 5847 - 18437 (+) polyketide synthase type I
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              • CAG23978.1
              • difG
              		 18456 - 24752 (+) polyketide synthase type I
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                • CAJ57408.1
                • difH
                		 24765 - 30518 (+) polyketide synthase type I
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                  • CAJ57409.1
                  • difI
                  		 30570 - 46184 (+) polyketide synthase type I
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                    • CAJ57410.1
                    • difJ
                    		 46189 - 53907 (+) polyketide synthase type I
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                      • CAJ57411.1
                      • difK
                      		 53930 - 60082 (+) polyketide synthase type I
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                        • CAG23983.1
                        • difL
                        		 60079 - 66294 (+) polyketide synthase type I
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                          • CAG23984.1
                          • difM
                          		 66370 - 67530 (+) putative cytochrome P450 monooxygenase
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                            • CAG23985.1
                            • difN
                            		 67588 - 68835 (+) 3-hydroxy-3-methylglutaryl coenzyme A synthase-like
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                              • CAG23986.1
                              • difO
                              		 68895 - 69641 (+) Enoyl-CoA hydratase
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                                Biosynthesis information

                                Biosynthetic modules

                                Name
                                1
                                Type
                                pks-trans-at
                                Genes
                                difF
                                Substrates
                                Integrated Monomers
                                Domains
                                ketosynthase, dehydratase, ketoreductase, methyltransferase, carrier (ACP)
                                Name
                                2
                                Type
                                pks-trans-at
                                Genes
                                difF
                                Substrates
                                Integrated Monomers
                                Domains
                                ketosynthase, dehydratase, ketoreductase
                                Name
                                3
                                Type
                                pks-trans-at
                                Genes
                                difF difG
                                Substrates
                                Integrated Monomers
                                Domains
                                ketosynthase, carrier (ACP)
                                Name
                                4
                                Type
                                pks-trans-at
                                Genes
                                difG
                                Substrates
                                Integrated Monomers
                                Domains
                                ketosynthase, ketoreductase, carrier (ACP)
                                Name
                                5
                                Type
                                pks-trans-at
                                Genes
                                difG difH
                                Substrates
                                Integrated Monomers
                                Domains
                                ketosynthase, dehydratase, carrier (ACP)
                                Name
                                6
                                Type
                                pks-trans-at
                                Genes
                                difH difI
                                Substrates
                                Integrated Monomers
                                Domains
                                ketosynthase, dehydratase, ketoreductase, methyltransferase, carrier (ACP)
                                Name
                                7
                                Type
                                pks-trans-at
                                Genes
                                difI
                                Substrates
                                Integrated Monomers
                                Domains
                                ketosynthase, ketoreductase, carrier (ACP)
                                Name
                                8
                                Type
                                pks-trans-at
                                Genes
                                difI
                                Substrates
                                Integrated Monomers
                                Domains
                                ketosynthase, dehydratase, ketoreductase, carrier (ACP)
                                Name
                                9
                                Type
                                pks-trans-at
                                Genes
                                difI
                                Substrates
                                Integrated Monomers
                                Domains
                                ketosynthase, ketoreductase, carrier (ACP)
                                Name
                                10
                                Type
                                pks-trans-at
                                Genes
                                difI difJ
                                Substrates
                                Integrated Monomers
                                Domains
                                ketosynthase, dehydratase, carrier (ACP)
                                Name
                                11
                                Type
                                pks-trans-at
                                Genes
                                difJ difK
                                Substrates
                                Integrated Monomers
                                Domains
                                ketosynthase, dehydratase, dehydratase
                                Name
                                12
                                Type
                                pks-trans-at
                                Genes
                                difK difL
                                Substrates
                                Integrated Monomers
                                Domains
                                ketosynthase, dehydratase, enoylreductase, methyltransferase, carrier (ACP)
                                Name
                                13
                                Type
                                pks-trans-at
                                Genes
                                difL
                                Substrates
                                Integrated Monomers
                                Domains
                                ketosynthase, thioesterase, carrier (ACP)
                                Annotation changelog

                                Entry version: 4

                                Date
                                Changes
                                Submitters
                                Reviewers
                                2022-10-07
                                Update chemical activity to schema version 2.11
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                Entry version: 3

                                Date
                                Changes
                                Submitters
                                Reviewers
                                2022-09-15
                                Removed invalid NRP information
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                2022-09-15
                                Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                2022-09-15
                                Reworked PKS modules to match reference paper
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                2022-09-15
                                Updated bioactivity data
                                • (ID: 77MNCE2XEGC43DNOUCFLS2KM)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                Entry version: 2

                                Date
                                Changes
                                Submitters
                                Reviewers
                                2019-10-16
                                Migrated from v1.4
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                2019-10-16
                                Updated compound(s) information (NPAtlas curation)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                Entry version: 1

                                Date
                                Changes
                                Submitters
                                Reviewers
                                2015-06-12
                                Submitted
                                • (ID: FICEQDRJCNKWHHGNCRVDDP5D)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                Detailed domain annotation
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