Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000182.1:
Cluster 1Polyketide175137

BGC0000182, mupirocin biosynthetic gene cluster from Pseudomonas fluorescens. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Pseudomonic acid A
ChemSpider ID: 393914
SMILES string: Copy to clipboard
Molecular formula:
Average molecular mass: Da
Molecular activity: Antibacterial
Molecular target: Isoleucyl tRNA synthetase

Class-specific details

Biosynthetic class(es):
Polyketide
Polyketide subclass:
Other (linear)
Polyketide synthase subclass:
Iterative type I / Trans-AT type I
Starter unit:
Acetyl-CoA
Polyketide synthase / ketosynthase-encoding genes:
Iterative PKS subtype:
Highly reducing
Number of iterations:
3
1
Thioesterase type:
Type I
Release / cyclization type:
Hydrolysis

Modular polyketide synthases:
mmpA (AAM12909.2)
Module 5
Scaffold-modifying domain: None
Module 6
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 7
Scaffold-modifying domain: Beta-branching

mmpB (AAM12911.1)
Module 8
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module is iterated
Evidence for iteration: Unknown

mmpD (AAM12913.2)
Module 1
KR stereochemistry: Unknown
Scaffold-modifying domain: Methylation
Module 2
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 3
KR stereochemistry: Unknown
Scaffold-modifying domain: Methylation
Module 4
KR stereochemistry: Unknown
Scaffold-modifying domain: None

Gene cluster description

mupirocin (BGC0000182). Gene Cluster 1. Biosynthetic class = Polyketide. GenBank AF318063. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Gene expression correlated with compound production, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Christopher Morton Thomas (University of Birmingham)

Literature references

1. El-Sayed AK et al. (2001) Quorum-sensing-dependent regulation of biosynthesis of the polyketide antibiotic mupirocin in Pseudomonas fluorescens NCIMB 10586. Microbiology 147(Pt 8):2127-39. doi: 10.1099/00221287-147-8-2127.
2. El-Sayed AK et al. (2003) Characterization of the mupirocin biosynthesis gene cluster from Pseudomonas fluorescens NCIMB 10586. Chem Biol 10(5):419-30.
3. Rahman AS et al. (2005) Tandemly duplicated acyl carrier proteins, which increase polyketide antibiotic production, can apparently function either in parallel or in series. J Biol Chem 280(8):6399-408. doi: 10.1074/jbc.M409814200. Epub 2004
4. Cooper SM et al. (2005) Mupirocin W, a novel pseudomonic acid produced by targeted mutation of the mupirocin biosynthetic gene cluster. Chem Commun (Camb) (9):1179-81. doi: 10.1039/b414781b. Epub 2005 Jan
5. Cooper SM et al. (2005) Shift to Pseudomonic acid B production in P. fluorescens NCIMB10586 by mutation of mupirocin tailoring genes mupO, mupU, mupV, and macpE. Chem Biol 12(7):825-33. doi: 10.1016/j.chembiol.2005.05.015.
6. Hothersall J et al. (2007) Mutational analysis reveals that all tailoring region genes are required for production of polyketide antibiotic mupirocin by pseudomonas fluorescens: pseudomonic acid B biosynthesis precedes pseudomonic acid A. J Biol Chem 282(21):15451-61. doi: 10.1074/jbc.M701490200. Epub 2007
7. Wu J et al. (2007) Mupirocin H, a novel metabolite resulting from mutation of the HMG-CoA synthase analogue, mupH in Pseudomonas fluorescens. Chem Commun (Camb) (20):2040-2.
8. Shields JA et al. (2010) Phosphopantetheinylation and specificity of acyl carrier proteins in the mupirocin biosynthetic cluster. Chembiochem 11(2):248-55. doi: 10.1002/cbic.200900565.
9. Hothersall J et al. (2011) Manipulation of quorum sensing regulation in Pseudomonas fluorescens NCIMB 10586 to increase mupirocin production. Appl Microbiol Biotechnol 90(3):1017-26. doi:
10. Gao SS et al. (2014) Biosynthesis of mupirocin by Pseudomonas fluorescens NCIMB 10586 involves parallel pathways. J Am Chem Soc 136(14):5501-7. doi: 10.1021/ja501731p. Epub 2014 Mar