BGC0000186: thailandamide biosynthetic gene cluster from Burkholderia thailandensis E264
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Location: 1,950,416 - 2,036,975 nt. (total: 86,560 nt).
This entry is originally from NCBI GenBank CP000085.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000186
Short description thailandamide biosynthetic gene cluster from Burkholderia thailandensis E264
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Type I)
Loci
CP000085.1
1950416 - 2036975
via Knock-out studies, Gene expression correlated with compound production
Compounds
  • thailandamide
  • thailandamide lactone
Species Burkholderia thailandensis E264 [taxonomy]
References
Chemical products information
thailandamide Evidence:
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C43H59N1O8
Chemical database entries
NPAtlas
thailandamide lactone Evidence:
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C43H55N1O8
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • BTH_II1663
  • ABC36202.1
 1950416 - 1953928 (-) pyruvate ferredoxin/flavodoxin oxidoreductase family protein
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    • BTH_II1664
    • ABC34599.1
    		 1953925 - 1960173 (-) polyketide synthase, putative
    • Scaffold biosynthesis
    • Knock-out
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    • BTH_II1665
    • ABC33986.1
    		 1960098 - 1974047 (-) polyketide synthase, putative
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      • BTH_II1666
      • ABC34675.1
      		 1973189 - 1989889 (-) polyketide synthase, putative
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        • BTH_II1667
        • ABC34176.1
        		 1989915 - 1995620 (-) polyketide synthase, putative
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          • BTH_II1668
          • ABC35867.1
          		 1995632 - 1996378 (-) polyketide biosynthesis enoyl-CoA hydratase
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            • BTH_II1669
            • ABC35267.1
            		 1996375 - 1997175 (-) polyketide biosynthesis enoyl-CoA hydratase
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              • BTH_II1670
              • ABC34601.1
              		 1997172 - 1998428 (-) polyketide biosynthesis protein
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                • BTH_II1671
                • ABC34518.1
                		 1998412 - 1999665 (-) polyketide beta-ketoacyl synthase, putative
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                  • BTH_II1672
                  • ABC35804.1
                  		 1999658 - 1999906 (-) acyl carier protein-related protein
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                    • BTH_II1673
                    • ABC35522.1
                    		 1999941 - 2011718 (-) thiotemplate mechanism natural product synthetase
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                      • BTH_II1674
                      • ABC34832.1
                      		 2011510 - 2028396 (-) polyketide synthase
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                        • BTH_II1675
                        • ABC34740.1
                        		 2028449 - 2031913 (-) trans acyltransferase (AT-AT-ER)
                        • Scaffold biosynthesis
                        • Knock-out
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                        • BTH_II1676
                        • ABC34917.1
                        		 2031959 - 2032756 (-) 4-phosphopantetheinyl transferase family protein
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                          • BTH_II1677
                          • ABC35757.1
                          		 2033299 - 2033553 (+) phenolpthiocerol synthesis type-i polyketide synthase ppsa
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                            • BTH_II1678
                            • ABC35295.1
                            		 2033561 - 2034322 (-) Ser/Thr protein phosphatase family
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                              • BTH_II1679
                              • ABC35022.1
                              • accA-2
                              		 2034376 - 2035344 (-) acetyl-CoA carboxylase, carboxyl transferase, alpha subunit
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                                • BTH_II1680
                                • ABC34496.1
                                		 2035673 - 2036224 (-) hypothetical protein
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                                  • BTH_II1681
                                  • ABC34138.1
                                  		 2036259 - 2036975 (-) autoinducer-binding transcriptional regulator, LuxR family, putative
                                  • Regulation
                                  • Knock-out Other in vivo study
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                                  Biosynthesis information

                                  Biosynthetic modules

                                  Name
                                  4
                                  Type
                                  nrps-type1
                                  Genes
                                  BTH_II1673
                                  Substrates
                                  D-alanine (evidence: Sequence-based prediction)
                                  Integrated Monomers
                                  Domains
                                  condensation (LCL), adenylation
                                  Name
                                  1
                                  Type
                                  pks-trans-at
                                  Genes
                                  BTH_II1674
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, ketoreductase, carrier (ACP)
                                  Name
                                  2
                                  Type
                                  pks-trans-at
                                  Genes
                                  BTH_II1674
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, ketoreductase, dehydratase, carrier (ACP)
                                  Name
                                  3
                                  Type
                                  pks-trans-at
                                  Genes
                                  BTH_II1674
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, ketoreductase, dehydratase, methyltransferase, carrier (ACP)
                                  Name
                                  5
                                  Type
                                  pks-trans-at
                                  Genes
                                  BTH_II1673
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, ketoreductase, dehydratase
                                  Name
                                  6
                                  Type
                                  pks-trans-at
                                  Genes
                                  BTH_II1673
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, carrier (ACP)
                                  Name
                                  7
                                  Type
                                  pks-trans-at
                                  Genes
                                  BTH_II1667
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, ketoreductase, dehydratase, carrier (ACP)
                                  Name
                                  8
                                  Type
                                  pks-trans-at
                                  Genes
                                  BTH_II1667
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase
                                  Name
                                  9
                                  Type
                                  pks-trans-at
                                  Genes
                                  BTH_II1667
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, ketoreductase, carrier (ACP)
                                  Name
                                  10
                                  Type
                                  pks-trans-at
                                  Genes
                                  BTH_II1666
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, ketoreductase, dehydratase, carrier (ACP)
                                  Name
                                  11
                                  Type
                                  pks-trans-at
                                  Genes
                                  BTH_II1666
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, carrier (ACP)
                                  Name
                                  12
                                  Type
                                  pks-trans-at
                                  Genes
                                  BTH_II1665
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, ketoreductase, dehydratase, carrier (ACP)
                                  Name
                                  13
                                  Type
                                  pks-trans-at
                                  Genes
                                  BTH_II1665
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, ketoreductase, dehydratase, methyltransferase, carrier (ACP)
                                  Name
                                  14
                                  Type
                                  pks-trans-at
                                  Genes
                                  BTH_II1664
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, methyltransferase, carrier (ACP)
                                  Annotation changelog

                                  Entry version: 4

                                  Date
                                  Changes
                                  Submitters
                                  Reviewers
                                  2022-10-07
                                  Update chemical activity to schema version 2.11
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                  Entry version: 3

                                  Date
                                  Changes
                                  Submitters
                                  Reviewers
                                  2022-09-15
                                  Corrected NRP module activity
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  2022-09-15
                                  Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                  Entry version: 2

                                  Date
                                  Changes
                                  Submitters
                                  Reviewers
                                  2019-10-16
                                  Migrated from v1.4
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  2019-10-16
                                  Updated compound(s) information (NPAtlas curation)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                  Entry version: 1

                                  Date
                                  Changes
                                  Submitters
                                  Reviewers
                                  2015-06-12
                                  Submitted
                                  • (ID: F65XGYSSLMUEJC2Q4T3BLCPS)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  Detailed domain annotation
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