BGC0000191 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000191 (Streptomyces galilaeus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	PKS-like,T2PKS	1	25,883	High	aclacinomycin	PKS

BGC0000191 - Region 1 - PKS-like,T2PKS

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Location: 1 - 25,883 nt. (total: 25,883 nt) Show pHMM detection rules used

Region on contig edge.

PKS-like: (t2pks2 or ksIII)
T2PKS: (t2ks and t2clf)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 25,884
Label		BGC0000191

Tailoring functions

Oxidoreductases: dehydrogenase oxidase

aclO: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

aknA: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase aminotransferase glycosyltransferase methyltransferase transferase

aclK: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

aclP: methyltransferase

smcogs: SMCOG1248: methyltransferase

aclY: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

aclZ: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

aknF: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

aknG: methyltransferase

smcogs: SMCOG1089: methyltransferase

Lyases: cyclase dehydratase

aclM: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

aknE1: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

Ligases: synthase

aknB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

aknC: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

aknE2: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000191.5	2.00	PKS	aclacinomycin	Streptomyces galilaeus
BGC0000192.3	1.51	PKS	aclacinomycin	Streptomyces galilaeus
BGC0000212.5	0.90	PKS	cinerubin B	Streptomyces sp. SPB074
BGC0000217.5	0.77	PKS	daunorubicin	Streptomyces sp.
BGC0000218.5	0.73	PKS	doxorubicin	Streptomyces peucetius
BGC0000231.5	0.69	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.66	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002154.2	0.61	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002175.3	0.59	PKS	YWA1	Aspergillus oryzae RIB40
BGC0001244.3	0.59	PKS	(-)-Mellein	Parastagonospora nodorum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000191.5	1.00	PKS	aclacinomycin	Streptomyces galilaeus
BGC0000192.3	0.91	PKS	aclacinomycin	Streptomyces galilaeus
BGC0000212.5	0.75	PKS	cinerubin B	Streptomyces sp. SPB074
BGC0000217.5	0.71	PKS	daunorubicin	Streptomyces sp.
BGC0000218.5	0.69	PKS	doxorubicin	Streptomyces peucetius
BGC0000231.5	0.68	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.66	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002154.2	0.64	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002175.3	0.63	PKS	YWA1	Aspergillus oryzae RIB40
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum

Similar known clusters from MIBiG 4.0

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

aknF: mal

PKS_AT (13..297): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 43.2
Methylmalonyl-CoA: 34.3
Methoxymalonyl-CoA: 31.9
Propionyl-CoA: 20.9
2-Methylbutyryl-CoA: 10.8
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 25883
Starter unit(s):: propionyl-CoA (Score: 1392.0; E-value: 0)
Malonyl elongations:: 8|9 (Score: 684.9; E-value: 6.9e-209)
Product class(es):: anthracycline
Product molecular weight(s):: propionyl-CoA_8: 558 Da
propionyl-CoA_9: 600 Da

aclK: GT (Score: 506.7; E-value: 1.3e-154)
aknA: KR C9 (Score: 489.1; E-value: 6.5e-150)
aknB: KS (Score: 724.5; E-value: 8e-221)
aknC: CLF 8|9 (Score: 684.9; E-value: 6.9e-209)
aknD: ACP (Score: 108.3; E-value: 3.5e-34)
aknE1: CYC C7-C12 (Score: 519.6; E-value: 5e-159)
aknE2: KSIII (Score: 529.9; E-value: 6e-162); KSIII BAB72048.1_Aklavinone_propionyl-CoA (Score: 729.0; E-value: 0.0)
aknF: AT (Score: 480.1; E-value: 8.3e-147); AT BAB72049.1_Aklavinone_propionyl-CoA (Score: 663.0; E-value: 0.0)
aknG: MET carboxy_O (Score: 429.9; E-value: 7e-132)
aknH: CYC C2-C19 (Score: 289.9; E-value: 5.9e-90)