BGC0000202 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000202 (Kibdelosporangium sp. MJ126-NF4)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T2PKS	1	50,393	High	azicemicin B	PKS

BGC0000202 - Region 1 - T2PKS

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Location: 1 - 50,393 nt. (total: 50,393 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 50,394
Label		BGC0000202

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase oxygenase

azicD: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

azicF: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

azicO1: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

azicO2: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

azicO3: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

azicO4: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

azicO5: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

azicO6: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

azicO7: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

azicQ: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

azicS: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: PPTase acylase methyltransferase transferase

azicG: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

azicK: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

azicL: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

azicT: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Lyases: cyclase decarboxylase

azicE: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

azicJ: Polyketide synthesis cyclase

smcogs: SMCOG1225: Polyketide synthesis cyclase

azicN: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

Ligases: ligase synthase

azicA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

azicB: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

azicM: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

azicV: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000202.5	0.98	PKS	azicemicin B	Kibdelosporangium sp. MJ126-NF4
BGC0000231.5	0.83	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.69	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001284.4	0.65	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000272.3	0.62	PKS	spore pigment	Streptomyces collinus
BGC0002276.2	0.61	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001250.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000202.5	0.98	PKS	azicemicin B	Kibdelosporangium sp. MJ126-NF4
BGC0000231.5	0.83	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.69	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001284.4	0.65	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000272.3	0.62	PKS	spore pigment	Streptomyces collinus
BGC0002276.2	0.61	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001250.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae

Similar known clusters from MIBiG 4.0

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

azicK: mal

PKS_AT (8..295): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 90.7
Methylmalonyl-CoA: 44.3
Methoxymalonyl-CoA: 41.1
Propionyl-CoA: 33.0
inactive: 16.2
Ethylmalonyl-CoA: 16.1
Acetyl-CoA: 15.4
CHC-CoA: 11.8
Isobutyryl-CoA: 11.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

azicM: Asp

Search NORINE for peptide: strict or relaxed

AMP-binding (8..400): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DYYPTTMRAK
Nearest Stachelhaus code(s):: Asp DYYPTTMRAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

azicV: Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (8..397): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DPYNMCIVTK
Nearest Stachelhaus code(s):: Cys DPYNMCIVTK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 50393
Starter unit(s):: aziridinyl-CoA (Score: 984.0; E-value: 0)
acetyl-CoA (Score: 73.2; E-value: 3.05e-101)
propionyl-CoA (Score: 73.2; E-value: 3.05e-101)
isobutyryl-CoA (Score: 73.2; E-value: 3.05e-101)
butyryl-CoA (Score: 73.2; E-value: 3.05e-101)
Malonyl elongations:: 8|9 (Score: 698.5; E-value: 5.1e-213)
Product class(es):: angucycline
Product molecular weight(s):: acetyl-CoA_8: 414 Da
acetyl-CoA_9: 456 Da
aziridinyl-CoA_8: 458 Da
aziridinyl-CoA_9: 500 Da
butyryl-CoA_8: 442 Da
butyryl-CoA_9: 484 Da
isobutyryl-CoA_8: 442 Da
isobutyryl-CoA_9: 484 Da
propionyl-CoA_8: 428 Da
propionyl-CoA_9: 470 Da

azicA: KS (Score: 748.6; E-value: 4e-228)
azicB: CLF 8|9 (Score: 698.5; E-value: 5.1e-213)
azicC: ACP (Score: 131.7; E-value: 1.6e-41)
azicD: KR C9 (Score: 471.8; E-value: 1.2e-144)
azicE: CYC C7-C12 (Score: 491.7; E-value: 1.5e-150)
azicF: KR C15 (Score: 380.0; E-value: 1.1e-116)
azicJ: CYC C4-C17/C2-C19 (Score: 161.4; E-value: 5e-50)
azicK: AT (Score: 368.9; E-value: 5.3e-113); AT AAG30190.1_R1128_acetyl-CoA/propionyl-CoA/isobutyryl-CoA/butyryl-CoA (Score: 293.0; E-value: 3.05e-101)
azicL: MET (Score: 271.3; E-value: 2.7e-83)
azicM: AT (Score: 294.3; E-value: 2.6e-90); AT ADB02855.1_Azicemicin_aziridinyl-CoA (Score: 984.0; E-value: 0.0)
azicO3: OXY (Score: 572.3; E-value: 2.1e-174)
azicO4: OXY (Score: 591.8; E-value: 2.5e-180)
azicO5: OXY (Score: 552.2; E-value: 2.6e-168)
azicO7: OXY (Score: 126.4; E-value: 3.6e-39)