LOCUS       BGC0000205             10111 bp    DNA     linear   BCT 15-JAN-2007
DEFINITION  Candidatus Endobugula sertula isolate DEEP transposase-like protein
            gene, complete cds; bryostatin PQRS gene cluster, complete sequence;
            and oxidoreductase gene, complete cds.
ACCESSION   BGC0000205
VERSION     BGC0000205
KEYWORDS    .
SOURCE      Candidatus Endobugula sertula (Bugula neritina bacterial symbiont)
  ORGANISM  Candidatus Endobugula sertula
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae; Candidatus Endobugula.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:10:56
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 10111
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..10111
                     /db_xref="taxon:62101"
                     /isolate="DEEP"
                     /mol_type="genomic DNA"
                     /organism="Candidatus Endobugula sertula"
     protocluster    1..10111
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[5462:6725]"
                     /cutoff="20000"
                     /detection_rule="(Chal_sti_synt_C or Chal_sti_synt_N)"
                     /neighbourhood="20000"
                     /product="T3PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5463..6725
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(Chal_sti_synt_C or Chal_sti_synt_N)"
                     /neighbourhood="20000"
                     /product="T3PKS"
                     /protocluster_number="1"
     protocluster    1..10111
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[4217:5438]"
                     /cutoff="10000"
                     /detection_rule="(t2pks2 or ksIII)"
                     /neighbourhood="20000"
                     /product="PKS-like"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      4218..5438
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(t2pks2 or ksIII)"
                     /neighbourhood="20000"
                     /product="PKS-like"
                     /protocluster_number="2"
     cand_cluster    1..10111
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Chal_sti_synt_C or Chal_sti_synt_N)"
                     /detection_rules="(t2pks2 or ksIII)"
                     /kind="neighbouring"
                     /product="T3PKS"
                     /product="PKS-like"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     subregion       1..10111
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000205"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..10111
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T3PKS"
                     /product="PKS-like"
                     /region_number="1"
                     /rules="(Chal_sti_synt_C or Chal_sti_synt_N)"
                     /rules="(t2pks2 or ksIII)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             complement(1475..1906)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1213:transposase
                     (Score: 136.9; E-value: 1.2e-41)"
                     /gene_kind="other"
                     /product="transposase-like protein"
                     /protein_id="ABM63536.1"
                     /transl_table=11
                     /translation="METTMKIRRQILVDSKSIRFDSKSIRSVSRDTGLSRNTIRKYLQD
                     DSPPTYQRQQKVKQPQLEGYTLQLEQWYHADQQRPKRERRTAKHLFEQLQLEGYRGSYT
                     TVCRFIKRLKRQVSGTAEAYVPLCXDAGDAMQFDGVRRW"
     gene            2151..4052
                     /gene="bryP"
     CDS             2151..4052
                     /NRPS_PKS="Domain: PKS_AT (5-280). E-value: 3.9e-53. Score:
                     172.6. Matches aSDomain: nrpspksdomains_bryP_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_AT (319-608). E-value: 4.1e-66.
                     Score: 215.2. Matches aSDomain:
                     nrpspksdomains_bryP_PKS_AT.2"
                     /codon_start=1
                     /gene="bryP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021:malonyl CoA-acyl carrier protein transacylase
                     (Score: 332.2; E-value: 1.2e-100)"
                     /gene_kind="biosynthetic-additional"
                     /note="acyl transferase"
                     /product="BryP"
                     /protein_id="ABM63531.1"
                     /sec_met_domain="PKS_AT (E-value: 4.4e-67, bitscore: 215.2,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METIYLFPGQGSQHKEMGKYLFDKYPELIHQADQQLHYSIKELCL
                     EDPDQLLNKTQFTQPALYIINALSFLEKIELDSHKPSYVAGHSLGEYNALFAAGAFDFL
                     TGLKLVKKRGLFMEEAPKGAMAAIIGITHNQVKCILEDIPQKNIDIANINSEKQFIISG
                     LYDEIIACENSFTKMGANFILLNVSAAFHSRYMKDIEIKFEQYLQKFQLNPLRTPVISN
                     YSARPYPKENYRDYMVKQISHPVKWYESISWLIQQDHFEFEEVGPGRVLTNLTNQIKKT
                     PLHIKNNLSFHQNKSKENYQESTVKNLSISKSKKKIIFMYSGQGSQYYQMGKELYDNNS
                     LFRHHMNYCSNQLKDRLGVSLIDIIYDKSKKNEEFDNIIYTNPVLYIFGYSLTQVLIDK
                     GIKPDAFLGHSLGEYIAATVAGIISLEDGLNLILTQAQLLEKHCNTGKILNVFSPPDVY
                     YKNQNLFFNTPLACVNFSNNFSVSGYRYEIDLVKKELDKRKIFSSYLPVSHGFHSHAID
                     PIENYFKKYVSQIKYSANKLPIYSCYYTSELTQDNIDNMKNYLWEVIRNTINFEKLISS
                     SFQNTSNNIFIDTSPNAALSNSLKHGFNKKHRHFFTINQFGKNIESINHLINNLNCI"
     misc_feature    2151..8118
                     /note="bryostatin PQRS gene cluster"
     CDS_motif       2160..2213
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_bryP_0001"
                     /evalue="9.30E-06"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="bryP"
                     /protein_end="21"
                     /protein_start="3"
                     /score="18.0"
                     /tool="antismash"
                     /translation="IYLFPGQGSQHKEMGKYL"
     aSDomain        2166..2990
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_bryP_PKS_AT.1"
                     /evalue="3.90E-53"
                     /label="bryP_PKS_AT.1"
                     /locus_tag="bryP"
                     /protein_end="280"
                     /protein_start="5"
                     /score="172.6"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFPGQGSQHKEMGKYLFDKYPELIHQADQQLHYSIKELCLEDPDQ
                     LLNKTQFTQPALYIINALSFLEKIELDSHKPSYVAGHSLGEYNALFAAGAFDFLTGLKL
                     VKKRGLFMEEAPKGAMAAIIGITHNQVKCILEDIPQKNIDIANINSEKQFIISGLYDEI
                     IACENSFTKMGANFILLNVSAAFHSRYMKDIEIKFEQYLQKFQLNPLRTPVISNYSARP
                     YPKENYRDYMVKQISHPVKWYESISWLIQQDHFEFEEVGPGRVLTNLTNQIKK"
     CDS_motif       2388..2504
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_bryP_0002"
                     /evalue="1.60E-12"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="bryP"
                     /protein_end="118"
                     /protein_start="79"
                     /score="39.4"
                     /tool="antismash"
                     /translation="KPSYVAGHSLGEYNALFAAGAFDFLTGLKLVKKRGLFME"
     CDS_motif       2703..2729
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_bryP_0003"
                     /evalue="3.90E-02"
                     /label="PKSI-AT-M_m6"
                     /locus_tag="bryP"
                     /protein_end="193"
                     /protein_start="184"
                     /score="7.3"
                     /tool="antismash"
                     /translation="LNVSAAFHS"
     aSDomain        3108..3974
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_bryP_PKS_AT.2"
                     /evalue="4.10E-66"
                     /label="bryP_PKS_AT.2"
                     /locus_tag="bryP"
                     /protein_end="608"
                     /protein_start="319"
                     /score="215.2"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: inactive"
                     /tool="antismash"
                     /translation="MYSGQGSQYYQMGKELYDNNSLFRHHMNYCSNQLKDRLGVSLIDI
                     IYDKSKKNEEFDNIIYTNPVLYIFGYSLTQVLIDKGIKPDAFLGHSLGEYIAATVAGII
                     SLEDGLNLILTQAQLLEKHCNTGKILNVFSPPDVYYKNQNLFFNTPLACVNFSNNFSVS
                     GYRYEIDLVKKELDKRKIFSSYLPVSHGFHSHAIDPIENYFKKYVSQIKYSANKLPIYS
                     CYYTSELTQDNIDNMKNYLWEVIRNTINFEKLISSSFQNTSNNIFIDTSPNAALSNSLK
                     HGFNKKHR"
     CDS_motif       3111..3158
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_bryP_0004"
                     /evalue="3.00E-05"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="bryP"
                     /protein_end="336"
                     /protein_start="320"
                     /score="16.6"
                     /tool="antismash"
                     /translation="YSGQGSQYYQMGKELY"
     CDS_motif       3348..3467
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_bryP_0005"
                     /evalue="1.80E-19"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="bryP"
                     /protein_end="439"
                     /protein_start="399"
                     /score="61.9"
                     /tool="antismash"
                     /translation="GIKPDAFLGHSLGEYIAATVAGIISLEDGLNLILTQAQLL"
     CDS_motif       3663..3689
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_bryP_0006"
                     /evalue="6.10E-02"
                     /label="PKSI-AT-M_m6"
                     /locus_tag="bryP"
                     /protein_end="513"
                     /protein_start="504"
                     /score="6.8"
                     /tool="antismash"
                     /translation="LPVSHGFHS"
     gene            4218..5438
                     /gene="bryQ"
     CDS             4218..5438
                     /NRPS_PKS="Domain: PKS_KS(Enediyne-KS) (79-374). E-value:
                     4.9e-34. Score: 109.8. Matches aSDomain:
                     nrpspksdomains_bryQ_PKS_KS.1"
                     /codon_start=1
                     /gene="bryQ"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     PKS-like: t2pks2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 297.7; E-value:
                     2.5e-90)"
                     /gene_kind="biosynthetic"
                     /note="beta-ketoacyl synthase"
                     /product="BryQ"
                     /protein_id="ABM63532.1"
                     /sec_met_domain="t2pks2 (E-value: 6.4e-173, bitscore:
                     563.9, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNGNSPVITITGIGIISAIGQGKDEFKNALFDGKNYFDIMQRPG
                     RQNGTEFIGAEAHSFTPVDRLSQNKSHTISLSGQMALAALDEAWHDAELEKMNHHRIGL
                     IIGGSNIQQRELVITQEKYRNKLHFLRPSYGLSFLDTDLCGLCTSAFNIQGMAYTVGGA
                     SASGQLAIIEAAEAVKSGRVDACIALGAMMDISYWECQSLSTLGAMGSRKYALSPHLAC
                     RPFDTHHDGFIYGEACAALVIEHRDQCRKSLPYANLTGWALHMDANRHPDPSVYGESNV
                     INSALNKANLIAEQIDYINPHGSSSIIGDNTELQALRTCRLVHAAINATKSITGHSLTA
                     AGAVEVAATLLQMKHKRLHPTRNLDEPIDPEFNWIIDQVKRADIQHSLCLSIGFGGVNT
                     ALCLSQI"
     aSDomain        4455..5339
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_bryQ_PKS_KS.1"
                     /domain_subtypes="Enediyne-KS"
                     /evalue="4.90E-34"
                     /label="bryQ_PKS_KS.1"
                     /locus_tag="bryQ"
                     /protein_end="374"
                     /protein_start="79"
                     /score="109.8"
                     /tool="antismash"
                     /translation="MALAALDEAWHDAELEKMNHHRIGLIIGGSNIQQRELVITQEKYR
                     NKLHFLRPSYGLSFLDTDLCGLCTSAFNIQGMAYTVGGASASGQLAIIEAAEAVKSGRV
                     DACIALGAMMDISYWECQSLSTLGAMGSRKYALSPHLACRPFDTHHDGFIYGEACAALV
                     IEHRDQCRKSLPYANLTGWALHMDANRHPDPSVYGESNVINSALNKANLIAEQIDYINP
                     HGSSSIIGDNTELQALRTCRLVHAAINATKSITGHSLTAAGAVEVAATLLQMKHKRLHP
                     TRNLDEPIDPEFNW"
     gene            5463..6725
                     /gene="BryR"
     CDS             5463..6725
                     /codon_start=1
                     /gene="BryR"
                     /gene_functions="biosynthetic (rule-based-clusters) T3PKS:
                     Chal_sti_synt_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1043:hydroxymethylglutaryl-CoA synthase (Score: 482.3;
                     E-value: 1.8e-146)"
                     /gene_kind="biosynthetic"
                     /note="HMG-CoA synthase-like protein"
                     /product="BryR"
                     /protein_id="ABM63533.1"
                     /sec_met_domain="Chal_sti_synt_N (E-value: 4.2e-09,
                     bitscore: 24.8, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRYIGIESMNAFGGTAFLDVMKLAQHRQLEMSRFDNLLMHQKSVA
                     LPYEDPITFGVNAAKPIIDGLSDVEKNNIELLITCSESGIDFSKSMSTYIHKYLGLNRS
                     CRLFEIKQACYSGTGGFQMAINFILSRTSPGAKALIVATDISRFIIDKNNFEQVEDWSY
                     AEPSNGAGAVALLISESPHIYQVDIGANGYYGYEVMDTCRPEADGDAGDADLSLLSYLD
                     CCEHAFLEYQKRVEGVDYRHSFDYLAFHTPFGGMVKGAHRTMMRKIAKAQPHEIEEDFQ
                     RRVVPGLNYCQRVGNIMGATIFLSLASTICNGHYPSPKRLGCFSYGSGCCSEFYSGMVT
                     QEGAQTLKKFNIEKELNERYSLSMSEYDNLLIHSQCVKFGTRNTKLDLDIISGAKVSMN
                     GKQRLVLKEIKEFHRKYQWIN"
     gene            7021..8118
                     /gene="BryS"
     CDS             7021..8118
                     /codon_start=1
                     /gene="BryS"
                     /note="methyl transferase"
                     /product="BryS"
                     /protein_id="ABM63534.1"
                     /transl_table=11
                     /translation="MKPFSTYPDQYQQQPDNKVNIFKDQLKQLNHNMNVDHIAVYSSPN
                     CYYRMRAEFKVWHTGNTSHYAMYDKNKKEPVFLEKFPVAAQSINQLMPLVMAEITQHEM
                     LRKKLFQIEFLTTQTNDTLVSLIYHKSLNEQWVHTISPIKDKLGINIIGRSRKQKMILD
                     RDFVNEELTVNGKIFYYQQNEGSFTQPNAKVCEKMLAWAVNNSKNNHGDLLELYCGNGN
                     FTLPLSQNFNRVIATEVSKSSVHSAQKNIKKNNIDNVSILRMSSEDFSLAIDKIRPFRR
                     LKKYDLESYHFSTVFVDPPRAGLDDGTIEMIKRFENIIYISCNPKTLINNLDSLCRTHN
                     IEALAAFDQFPYTEHLEAGVILKKK"
     CDS             complement(8122..9885)
                     /codon_start=1
                     /product="oxidoreductase"
                     /protein_id="ABM63535.1"
                     /transl_table=11
                     /translation="MKTIILVMKKRWNSKQPRELTMNEFVLSLPPFAWFFIGALLLLVC
                     RGFIASAITIIMPIVSFMLLLQWDHGTDYAYPLMGMDLTLVRVDRLSLLFAYLFHLAVL
                     IGGIFALHEKDKVQQIAALAYAGSAIGAVFAGDWISLFVFWELLALTSVFLVWARRTSA
                     SYHSGMRYLIIQVLSGVLLFAGALLLIRQTGSMAFTDIGLSGIGAWLIFIAFGIKCGFP
                     FFHSWITDAYPEATPTGTVFLCAMTTKVAVYALARSFAGEDILVTIGLVMACFPIFFAV
                     IENDLRRVLAYSMINQIGFMVCGIGIGTEMAINGAVAHAFNDVIFKGLLMMSMGAVLFR
                     VGHVKGSHLGGLYRSMPKTTMCCIVGAASISAFPLFSGFISKSIIMLEMLEAGHTVGWL
                     LLLFAAAGVFHHAGIKIPFFAFFAHDSGLKVKEAPANMLIAMGIAAILCIFIGSYPSFL
                     YSLLPFPIHYSPYDVTHVLTQMQLLFFSALAFVWLNLKGLYPPELPSTNIDSEWLYRHV
                     APAIVSRVVVQVEGIYILMKTALLKALNHLIILLSYLSSPQSPLARAWSVNGMLLALLI
                     LMIVVLLFNYF"
ORIGIN
        1 tgttcttgtc caagcattga tagttatcag gagagttaat ctggataagt caatgtagcc
       61 aagcgcaggc tgataggggt agacgctaaa tggctagaaa catacctact gattttttgc
      121 gctcattcgt agtgattgct gaactgggga gctttaccca ggccggtaat caattggcac
      181 gatcacaatc tgcgatcagc ctgcaaataa aaaaattgga agaactatta ggtcaaaaga
      241 tctttgaccg taaaggccat aattttcagc taacccctac aggggaaacg ttgatgccct
      301 atgctaggca aattcttcag cttaatgacc gactgatgga tgaccttgac caaagagtga
      361 gtgagggaaa agtcaggctg ggtattccca gtgaatttgc gacggcgtta ctacctaaaa
      421 tcgttggtaa attttctcgg gaaaaccctc agattgcatt ggaggtcaaa tgtgatttga
      481 gccgtaattt aaatctagat attaagcaaa acaaatatga tctaatttta tcgcttgtcg
      541 atgatccaag tcaggttaaa ggtgaatata ttaggcgaga taaattagtc tgggtggtat
      601 ctccagagta ttatcttgat aatattacca ccttcccttt aattgttgcc ccaggaggtt
      661 gtatctaccg taacagagca atcaacacct taaatcgatg tcgtgtacct tggcaaatta
      721 tttataccat tatggacatt gccggtatta aagcagctat cgaagatagg cttggggatt
      781 actgtgttag cagaagtaca gtgcctcaaa atttgaaaac tatttcctcc tcagagcggc
      841 ttgaaaagct cggcgacatt ggtattagtt tgattagtta tggtgtccat atgtcagatg
      901 cgacccgagc gttggcagat tatttaaaac agtacttgga gtagagtatt gatgatgctt
      961 caagtctaat gatatctaga aattcaaata gtaagtttgg ttgatatctc acgcttgtca
     1021 aattgcactt aaagctgacc cggggtttgc atctaacact gacccaccgg tgaaagctac
     1081 tttcatgtct gttgctgact ctgcttaaaa cgatacgacg catgcttggt ttcgatgatt
     1141 gaacaatgat gcgtgatacg atccaataac gcggtggtca tcttggcatc accaaacaca
     1201 ctgttccatt ccccaaaagc cagatagtga tgcatcagag ccataaaccg gggatgaaag
     1261 tccctctgct taccctgacc aatgcgcact accatggttt tggggttatc aatcaatccc
     1321 cgctggggga caccctggta aaacgccaat gcatggttaa aggcatccag taacatttct
     1381 tgcgattccc gcaggcaggc gcggatacag cgttggcgac tatgtgatag gcgaaaatgg
     1441 gcaatacgca ccgtggtttc gacactaccg agtatcacca cctcctgact ccgtcaaact
     1501 gcatcgcatc acctgcatca angcataggg gcacataagc ctctgctgtg cctgatactt
     1561 gacgctttaa ccgcttgata aaccgacaga ccgtggtgta agaaccccga taaccctcaa
     1621 gctgcagttg ctcaaacaag tgtttagccg tacggcgctc acgcttaggt cgctgctgat
     1681 cggcgtgata ccactgttcc aactgaaggg tataaccctc caattgcggt tgtttgactt
     1741 tttgttgacg ttgatatgtc ggtggagaat cgtcttgtag atatttacga atcgtattac
     1801 gagacaatcc cgtatcacgg ctgactgaac gaatactttt actatcaaaa cgaatacttt
     1861 tactatcaac cagtatctgg cgacggattt tcatggttgt ttccatcaat aacacctatt
     1921 ttatctccgg gtaatacaaa cccgctattt aacgaaatag gtgggtcaat ttcacatgca
     1981 ctttccaacc taagtgggtc agttttgcat gctattcaac acattggtta ttaaagtgcc
     2041 aacatactga actgtgctgc aattcaggca gtctattatt ttttgtatgt acttaaagaa
     2101 aattttccta gataatacca attagtcatt gaaatataga ggtcatagtc gtggaaacta
     2161 tttatctttt ccctgggcag ggttcacagc acaaggaaat ggggaaatat ttatttgaca
     2221 aatatcctga actcatacac caagcagatc agcaacttca ttattctata aaagagcttt
     2281 gcttagaaga tcctgatcaa ttacttaata aaactcaatt tacacaacct gccctatata
     2341 ttattaatgc acttagtttc ttagaaaaga ttgaattaga ttcacataaa ccatcatatg
     2401 ttgcaggcca tagtttaggt gaatacaatg cactctttgc tgctggagcc tttgattttt
     2461 taaccggact aaagcttgtc aaaaaaaggg gattattcat ggaagaagca cctaaaggtg
     2521 caatggcagc aataattggc attacccata accaagtaaa atgtatatta gaagatattc
     2581 ctcaaaaaaa tattgatatc gctaatataa attcagagaa acagttcatt atctcaggtc
     2641 tttatgatga aataatagca tgcgaaaact cattcactaa aatgggggct aacttcatac
     2701 tacttaatgt tagcgctgcc tttcattcac gttatatgaa agatatagaa ataaaattcg
     2761 agcaatattt acaaaaattc caattaaacc ctttaagaac ccctgttata tcaaattata
     2821 gcgcgagacc ttatccgaaa gaaaactacc gggattatat ggttaaacag atatctcacc
     2881 ccgtcaaatg gtatgagtct atttcctggt tgatacaaca ggatcatttc gagttcgaag
     2941 aagttggccc aggaagagtg ttaacaaatc tgacaaatca aataaaaaaa acacctttac
     3001 atattaaaaa taatttatca ttccatcaaa ataaatctaa agaaaattac caagaatcta
     3061 ctgttaaaaa tctatctatt tcaaaaagta aaaagaaaat tatttttatg tattctggac
     3121 agggttcgca atattatcaa atgggcaaag agctttatga taataatagc ctatttaggc
     3181 atcatatgaa ttactgcagt aatcaactaa aggatagatt aggtgtatca ctgatcgata
     3241 tcatttatga taaatcaaaa aaaaatgaag aatttgacaa tattatttat actaatccgg
     3301 tattatatat ttttggttat agcctaacac aagtgcttat agataaagga ataaagccag
     3361 acgcattttt aggacatagc ttaggagaat atattgctgc aacagtagct ggtatcatat
     3421 cattggaaga cggccttaat ttgatcttga ctcaagctca attactggaa aaacattgta
     3481 atacaggaaa aatattaaat gtttttagtc cacctgatgt ttattataaa aaccaaaatt
     3541 tgttttttaa tacaccactt gcttgtgtca atttttctaa caatttttca gtcagcggtt
     3601 atcgttatga aatagatttg gttaagaaag aattggataa aagaaagatt ttttcatctt
     3661 atctccccgt atcgcatgga tttcattcac atgctataga tcccatagaa aattatttta
     3721 aaaaatatgt atctcaaata aaatatagtg ccaataagtt gccaatatat tcttgttatt
     3781 acacctccga gctaactcaa gacaatatag acaacatgaa aaattattta tgggaagtca
     3841 taagaaacac aatcaatttt gaaaagttaa tatcgtcttc ttttcagaac acaagcaata
     3901 atatttttat tgatacaagc cctaacgcag ctttatcaaa ctctttaaaa catgggttta
     3961 ataaaaagca taggcacttc tttaccatca accaatttgg taaaaatata gaatcaatca
     4021 atcatttaat aaataattta aattgtattt aatataaatg cattttctta attaagcatc
     4081 attgccatac ataattggaa gtattttttg ataaaaatca gtcgctttaa aacattgatg
     4141 gaccactcaa taattaatat tgaaaatagt tatgtttatt tcgaaaaaat tatcatgcga
     4201 attggtgaaa tgattaaatg aacaatggta actcgcctgt tattacaatc actggtatag
     4261 gcataatttc agcgattggc cagggcaaag atgaatttaa aaatgcacta tttgacggca
     4321 aaaactactt tgacattatg caaagacctg gcaggcaaaa tggtactgaa tttattggcg
     4381 cagaagcaca ttcgtttaca ccagtagata gattatcgca aaataaatca cacactatat
     4441 ccttatcggg gcaaatggca ttagctgcct tagacgaagc atggcatgat gcagagcttg
     4501 aaaaaatgaa tcatcaccgc atcggattaa ttataggtgg aagcaacatt caacaacgcg
     4561 aattggtaat cacccaagaa aaataccgta acaaacttca ttttttgcgt ccatcttatg
     4621 gtctatcatt tttagacact gatttatgcg gactatgcac atcggcattt aacatacaag
     4681 gtatggctta taccgtgggg ggagcatctg ccagtggtca attagccata attgaagcgg
     4741 ctgaagcggt taaatcggga cgagttgatg cctgtatcgc cctaggtgcc atgatggata
     4801 tctcctattg ggaatgccaa agtttgtcca cattaggtgc aatgggctcc cgtaaatatg
     4861 cactatcgcc ccatttggcc tgccgtcctt tcgatacaca ccatgatgga tttatttatg
     4921 gtgaagcttg tgctgcatta gtcatagagc atagagatca atgtaggaaa tccttacctt
     4981 acgctaacct tacaggctgg gccttgcata tggatgctaa ccgtcatcca gacccttctg
     5041 tatatggcga aagtaatgta ataaattcag cactgaataa agccaacttg atagctgagc
     5101 aaatagatta tattaacccc cacggcagct catcaattat tggtgataat acagaattac
     5161 aggcactacg aacttgtcga ttagtacacg ctgcaattaa tgcgactaaa tcaattaccg
     5221 gacatagctt aacggcagca ggagcagtag aagtggcagc aacattacta caaatgaaac
     5281 ataagcgatt acacccaacc cgaaaccttg atgaacccat tgacccagaa tttaactgga
     5341 ttatcgatca agtaaaaaga gcagatattc aacacagtct atgtttgagt ataggttttg
     5401 gaggggttaa cacggcactt tgccttagcc aaatttaaat attttaacaa agggtaacga
     5461 tcatgaggta tattggtata gaatcaatga atgcttttgg tggcacagca tttctggatg
     5521 ttatgaaact agcgcaacac cgccagttag aaatgtcccg ctttgacaat ttattgatgc
     5581 atcaaaaaag tgttgcacta ccctatgaag atcctattac tttcggcgta aatgcagcta
     5641 aaccaattat tgatggttta agcgacgttg agaaaaataa tattgaatta ttaattactt
     5701 gttcagaatc tggaattgat tttagcaaat ctatgagtac ctatattcac aagtacttgg
     5761 ggcttaatcg ctcttgccgt ttgtttgaaa taaagcaagc ctgttattca ggtactggcg
     5821 gattccaaat ggctattaac tttatcttat caagaacatc acctggcgca aaagctttaa
     5881 ttgtagcgac tgatatatca cgatttataa tagataaaaa taattttgaa caggtagaag
     5941 attggtctta tgccgaaccc agtaatggcg cgggtgctgt agcactatta atcagtgaat
     6001 cgccacatat ttatcaagtc gatataggag ctaatggata ttacgggtat gaggttatgg
     6061 atacttgtcg acccgaagct gatggtgacg ccggagatgc tgatctatct ttattatcct
     6121 atcttgattg ttgtgaacac gcatttttag aataccaaaa acgcgtcgaa ggtgtagatt
     6181 atcgtcattc ttttgattat ttggcatttc acacaccttt tggtggcatg gttaagggag
     6241 ctcatcgcac aatgatgcgt aaaatagcca aagcgcaacc tcacgaaatt gaagaagatt
     6301 ttcagcgacg tgttgttccc ggacttaatt attgccagcg tgtcggcaat attatgggag
     6361 ccacaatatt tttgtcattg gcaagtacta tttgtaatgg gcactaccct tctccaaagc
     6421 gtcttggatg tttttcttat ggctctggtt gctgttcgga attctatagc ggaatggtta
     6481 ctcaggaagg tgcacaaaca ttaaaaaaat ttaatataga aaaagagttg aacgaacgtt
     6541 attcactctc tatgtcggaa tatgataatc tattaatcca tagtcagtgt gtaaaatttg
     6601 gcactcgtaa tacaaaactt gacctggata tcattagcgg ggccaaagtc tcgatgaatg
     6661 gtaaacaacg gctagtacta aaagaaatta aagagtttca tagaaaatat cagtggatca
     6721 attaaaccag taaaaaccgc gaaaaaactt ggttttattc aactcatgca ccgtgaagaa
     6781 tgttaaacca ctactaaagt gagtgccctc atagagtttc atactctctt aatgaactct
     6841 cggctttata tatttttatt catcgactaa ttatacgatg ccatttagga acagttttct
     6901 gtggaagctc cctgtttccg aagtgcttaa atcaccagat attttttcaa gtgccttatt
     6961 attataaata ccttataatc tcgactatta agccgccact aacttcattt attttattcc
     7021 atgaaaccat tttcaactta tccagaccaa tatcaacaac agccagataa taaagtcaat
     7081 atcttcaaag accagctcaa acagttgaat cataatatga atgtcgacca tatagctgtt
     7141 tacagctcac caaactgcta ctatagaatg cgggccgagt ttaaagtctg gcatacaggt
     7201 aataccagcc attatgctat gtacgacaaa aacaagaaag agcctgtgtt tttagaaaag
     7261 tttccagtgg cggctcaatc gatcaatcaa ttaatgccac tagtcatggc agaaattacc
     7321 cagcatgaga tgttacgtaa aaaacttttc cagattgagt ttttaaccac acaaacaaat
     7381 gataccttag tatcattgat ttatcataaa tcattgaatg aacaatgggt tcataccata
     7441 tctcccatca aggataaact cggtatcaac attattggcc gtagccgcaa gcaaaaaatg
     7501 atactagacc gagattttgt caatgaagaa ctgactgtca atggaaaaat attttattac
     7561 cagcaaaatg aaggtagttt tactcagcct aatgccaaag tatgcgaaaa gatgttagca
     7621 tgggcggtga acaatagtaa aaataaccac ggtgacttat tagaacttta ttgtggcaac
     7681 ggtaatttca ccttgccttt atcgcaaaat tttaatcgtg ttattgccac tgaagtttca
     7741 aaatcgtctg ttcattctgc acagaaaaat attaaaaaaa acaatattga taacgtttca
     7801 attctgagaa tgtccagtga agacttttcc ttagcaatag ataaaatacg tccatttagg
     7861 cgattaaaaa aatatgattt ggaaagctat cacttttcaa ccgttttcgt agacccgcct
     7921 cgggccggtt tggatgacgg tactattgaa atgataaaac gctttgaaaa cattatttat
     7981 atctcctgca accctaagac cttgatcaac aatctagatt ctctctgtcg cactcacaat
     8041 attgaagcac tggcagcctt cgatcagttc ccctatactg aacatttaga agcaggggtt
     8101 attttaaaaa agaaataagt gctaaaaata attaaataac aacacaacaa tcattaatat
     8161 taatagcgcc aataacatgc cattaacact ccaggctcta gccaagggtg actggggtga
     8221 agatagatag gataacaata ttatcaaatg atttaatgcc ttaagcaagg cggttttcat
     8281 caagatataa ataccctcaa cctgaacaac aaccctagac acaatggctg gagctacatg
     8341 tcgatacaac cattcactat cgatatttgt cgacggtaat tctggtggat acaaaccttt
     8401 taaatttaac caaacaaagg ctaacgcaga gaagaataac aactgcattt gtgttaacac
     8461 atgggtcaca tcataaggag aataatggat gggaaaaggt aacaaggaat agagaaaact
     8521 tggatagcta ccaatgaaaa tacataaaat agctgcaatg cccatcgcaa tcaacatatt
     8581 agcaggcgct tctttgactt ttaaacctga atcatgagca aaaaaggcaa agaaagggat
     8641 tttaatgccc gcatgatgaa acacaccagc agcagcaaat aacaatagta accaaccaac
     8701 ggtatgccct gcttccagca tctccaacat aataatcgat ttactgataa aaccactaaa
     8761 caatgggaag gcagaaatcg atgcagcgcc cacaatacaa cacattgtgg tcttcggcat
     8821 actacgatag agtccgccaa ggtgggaacc cttgacatga cccactcgaa acaacaccgc
     8881 tcccatcgac atcattaaca gacctttaaa aatcacatcg ttaaaagcat gagccacagc
     8941 cccattaatc gccatttcag taccaatacc aataccacat accataaaac cgatctgatt
     9001 aatcatgcta taagcaagca ctcgacgcag atcattctca atcacagcaa agaagatagg
     9061 gaaacaagcc ataaccaaac caatggtcac gagtatatct tcaccagcga aactgcgagc
     9121 caaggcataa accgcaacct tcgtggtcat agcacacaaa aagacagtcc ccgtgggagt
     9181 tgcctcagga taagcatcag taatccagct atgaaaaaag ggaaagccac atttaatacc
     9241 aaaggcaata aagattaacc aagccccaat accactcaac ccgatatcgg taaatgccat
     9301 actgcctgtt tgtcgtatta acagcaaagc ccctgcaaat aataaaacac cagacaatac
     9361 ctgaatgatt aaataacgca taccactgtg gtagctagct gatgtgcgac gagcccaaac
     9421 caaaaaaacc gatgttaaag ccaataattc ccaaaaaaca aataagctaa tccagtcacc
     9481 cgcaaataca gctccgattg cactcccagc ataagccaag gcagcaatct gctgtacttt
     9541 atctttttca tgtaaggcaa aaatgccacc aatcaataca gccaaatgga ataaataagc
     9601 aaatagcaag ctcagacggt caactcgaac gagtgttaaa tccatcccca tcaatgggta
     9661 ggcatagtcg gttccatgat cccattgcag caataacata aaactcacaa taggcattat
     9721 gatcgtaata gcggaagcga taaaccctcg gcaaaccaac aataaaagag cgccgataaa
     9781 aaaccaagca aaaggtggca agcttaacac gaactcattc atcgtcagct ccctcggctg
     9841 tttggaattc catcgtttct tcatgacgag aataatagtc ttcatcacgc atcaatactt
     9901 tacgcatctg tgacgcaatc aataccaaaa tcacgcaggc aacaaaacca taaagcgcat
     9961 aaaaagcagg tattttttcc aatggggtaa tgatatgacg atgtacaacc aagtcaacaa
    10021 tcactaacac aatacatgac gtataaaaag ctctcagaat ccagcgtatg gtattcggtt
    10081 gataaaaaaa tccatcaggt tgattatgat c
//
