Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000210.1:
Cluster 1Polyketide / Saccharide142074

BGC0000210, chromomycin A3 biosynthetic gene cluster from Streptomyces griseus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: chromomycin A3
PubChem ID: 5351560
SMILES string: Copy to clipboard
Molecular formula: C57H82O26
Average molecular mass: 1183.257 Da
Molecular activity: Cytotoxic
Molecular target: DNA

Class-specific details

Biosynthetic class(es):
Polyketide / Saccharide
Polyketide subclass:
Other (cyclic)
Polyketide synthase subclass:
Type II
Starter unit:
Malonyl-CoA
Polyketide synthase / ketosynthase-encoding genes:
CAE17520.1,CAE17526.1
Genes involved in folding/cyclization:
CAE17518.1, CAE17525.1, CAE17552.1

Saccharide subclass:
oligosaccharide
Glycosyltransferases:
CAE17535.1:
Specificity: D-oliose
Evidence for specificity: Knock-out construct
CAE17545.1:
Specificity: D-olivose
Evidence for specificity: Knock-out construct
CAE17547.1:
Specificity: D-olivose
Evidence for specificity: Knock-out construct
CAE17548.1:
Specificity: D-oliose
Evidence for specificity: Knock-out construct

Gene cluster description

chromomycin A3 (BGC0000210). Gene Cluster 1. Biosynthetic class = Polyketide/Saccharide. GenBank AJ578458. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Carmen Mendez (University of Oviedo)

Literature references

1. Garcia B et al. (2011) The chromomycin CmmA acetyltransferase: a membrane-bound enzyme as a tool for increasing structural diversity of the antitumour mithramycin. Microb Biotechnol 4(2):226-38. doi: 10.1111/j.1751-7915.2010.00229.x.
2. Bosserman MA et al. (2011) Characterization of the terminal activation step catalyzed by oxygenase CmmOIV of the chromomycin biosynthetic pathway from Streptomyces griseus. Biochemistry 50(8):1421-8. doi: 10.1021/bi1016205. Epub 2011 Feb 2.
3. Menendez N et al. (2007) Involvement of a chromomycin ABC transporter system in secretion of a deacetylated precursor during chromomycin biosynthesis. Microbiology 153(Pt 9):3061-70. doi: 10.1099/mic.0.2007/007922-0.
4. Menendez N et al. (2006) Deoxysugar transfer during chromomycin A3 biosynthesis in Streptomyces griseus subsp. griseus: new derivatives with antitumor activity. Appl Environ Microbiol 72(1):167-77. doi:
5. Menendez N et al. (2004) Tailoring modification of deoxysugars during biosynthesis of the antitumour drug chromomycin A by Streptomyces griseus ssp. griseus. Mol Microbiol 53(3):903-15. doi: 10.1111/j.1365-2958.2004.04166.x.
6. Menendez N et al. (2004) Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. Chem Biol 11(1):21-32. doi: 10.1016/j.chembiol.2003.12.011.