BGC0000216 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000216 (Dactylosporangium sp. SC14051)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T2PKS,other	1	50,689	High	dactylocycline A	PKS

BGC0000216 - Region 1 - T2PKS,other

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Location: 1 - 50,689 nt. (total: 50,689 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)
other: (Neocarzinostat or bcpB or frbD or mitE or prnB or CaiA or bacilysin or orf2_PTase)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 50,690
Label		BGC0000216

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

dacJ: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

dacO1: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

dacO2: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

dacO3: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

dacS6: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: acylase aminotransferase carboxylase glycosyltransferase halogenase methyltransferase transferase

dacE: conventional flavin-dependent halogenase

halogenases: conventional flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

dacM1: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

dacM2: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

dacP: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

dacP1: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

dacP3: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

dacQ: aminotransferase

smcogs: SMCOG1019: aminotransferase

dacS1: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

dacS2: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

dacS8: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

Hydrolases: back translocase hydrolase

dacD: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

dacR1: GTP-binding protein LepA

smcogs: SMCOG1190: GTP-binding protein LepA

Lyases: cyclase dehydratase

dacK: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

dacS9: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

dacA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

dacB: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

dacH: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000216.5	1.53	PKS	dactylocycline A	Dactylosporangium sp. SC14051
BGC0001817.5	0.65	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0002274.2	0.51	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624
BGC0000231.5	0.43	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000749.3	0.41	saccharide	capsular polysaccharide	Escherichia coli
BGC0000234.5	0.37	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0000898.5	0.36	saccharide	desosamine, pikromycin, neomethymycin, narbomycin	Streptomyces venezuelae
BGC0001374.5	0.35	other (other)	thienodolin	Streptomyces albogriseolus
BGC0002284.3	0.35	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0002458.3	0.34	NRPS	alazopeptin	Streptacidiphilus griseoplanus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000216.5	0.99	PKS	dactylocycline A	Dactylosporangium sp. SC14051
BGC0001817.5	0.68	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0000231.5	0.62	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001254.3	0.61	PKS	ACT-Toxin II	Alternaria alternata
BGC0000272.3	0.60	PKS	spore pigment	Streptomyces collinus
BGC0002274.2	0.59	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624
BGC0000234.5	0.55	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001197.5	0.54	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0000271.5	0.53	PKS	spore pigment	Streptomyces avermitilis

Similar known clusters from MIBiG 4.0

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

dacP: mal

PKS_AT (9..301): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 60.9
Methoxymalonyl-CoA: 35.7
Methylmalonyl-CoA: 31.1
inactive: 15.0
Propionyl-CoA: 14.9
Ethylmalonyl-CoA: 11.5
2-Methylbutyryl-CoA: 11.3
fatty_acid: 9.0
Isobutyryl-CoA: 8.7
CHC-CoA: 7.7
trans-1,2-CPDA: 7.5
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

dacH: X

AMP-binding (29..427): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TFMHLGGPSK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 50689
Starter unit(s):: malonamyl-CoA (Score: 2017.0; E-value: 0)
Malonyl elongations:: 8|9 (Score: 657.8; E-value: 1.2e-200)
Product class(es):: anthracycline
aureolic acid
tetracycline
Product molecular weight(s):: malonamyl-CoA_8: 667 Da
malonamyl-CoA_9: 709 Da

dacA: KS (Score: 741.3; E-value: 6.3e-226)
dacB: CLF 8|9 (Score: 657.8; E-value: 1.2e-200)
dacC: ACP (Score: 115.0; E-value: 2.8e-36)
dacD: AMID (Score: 1089.8; E-value: 0.0); AMID ELQ83270.1_Oxytetracycline_malonamyl-CoA (Score: 851.0; E-value: 0.0)
dacE: HAL (Score: 732.7; E-value: 3.7e-223)
dacG: CYC C1-C18|C2-C19 (Score: 233.9; E-value: 1.2e-72)
dacH: LIG (Score: 685.8; E-value: 7.4e-209); LIG ELQ83297.1_Oxytetracycline_malonamyl-CoA (Score: 553.0; E-value: 0.0)
dacK: CYC C7-C12 (Score: 352.8; E-value: 3e-108)
dacM1: MET C6|C8 (Score: 575.8; E-value: 5.3e-176)
dacM2: MET C2O|C2N (Score: 621.0; E-value: 1.1e-189)
dacM3: MET C9O (Score: 437.6; E-value: 1.7e-134)
dacN: CYC C5-C14/C3-C16 (Score: 434.2; E-value: 3.7e-133)
dacO1: OXY (Score: 464.0; E-value: 1.5e-141)
dacO2: OXY (Score: 458.8; E-value: 5.3e-140)
dacO3: OXY (Score: 488.8; E-value: 4.2e-149)
dacP: AT (Score: 433.9; E-value: 8.9e-133); AT AFU65899.1_Dactylocycline_malonamyl-CoA (Score: 613.0; E-value: 0.0)
dacQ: AMIN (Score: 622.2; E-value: 6.1e-190)
dacS8: GT (Score: 360.6; E-value: 3.2e-110)