LOCUS       BGC0000217              8089 bp    DNA     linear   BCT 14-FEB-1996
DEFINITION  Streptomyces sp. aklaviketone reductase (dauE) gene;
            cyclase/dehydrase (dauA) gene; polyketide reductase (dauB) gene;
            oxygenase, polyketide synthase, acyltransferase (dauA) genes;
            aklanonic acid methyltransferase (dauC) gene.
ACCESSION   BGC0000217
VERSION     BGC0000217
KEYWORDS    .
SOURCE      Streptomyces sp
  ORGANISM  Streptomyces sp.
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 17:46:30
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8089
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..236
                     /gene="dauE"
     source          1..8089
                     /db_xref="taxon:1931"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp."
                     /strain="C5"
     protocluster    1..8089
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[2708:5248]"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_malonyl_elongations="8|9 (Score: 616.0; E-value:
                     5.9e-188)"
                     /t2pks_molecular_weights="propionyl-CoA_8 (Da): 394.416"
                     /t2pks_molecular_weights="propionyl-CoA_9 (Da): 436.452"
                     /t2pks_product_classes="angucycline"
                     /t2pks_product_classes="anthracycline"
                     /t2pks_product_classes="aureolic acid"
                     /t2pks_product_classes="tetracycline"
                     /t2pks_starter_units="propionyl-CoA (Score: 1250.0;
                     E-value: 0)"
                     /tool="antismash"
     proto_core      2709..5248
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_malonyl_elongations="8|9 (Score: 616.0; E-value:
                     5.9e-188)"
                     /t2pks_molecular_weights="propionyl-CoA_8 (Da): 394.416"
                     /t2pks_molecular_weights="propionyl-CoA_9 (Da): 436.452"
                     /t2pks_product_classes="angucycline"
                     /t2pks_product_classes="anthracycline"
                     /t2pks_product_classes="aureolic acid"
                     /t2pks_product_classes="tetracycline"
                     /t2pks_starter_units="propionyl-CoA (Score: 1250.0;
                     E-value: 0)"
     cand_cluster    1..8089
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(t2ks and t2clf)"
                     /kind="single"
                     /product="T2PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..8089
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000217"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8089
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T2PKS"
                     /region_number="1"
                     /rules="(t2ks and t2clf)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             <1..236
                     /codon_start=3
                     /gene="dauE"
                     /note="putative"
                     /product="aklaviketone reductase"
                     /protein_id="AAA87614.1"
                     /transl_table=11
                     /translation="IRVIGVAPGVIETGAGVRAGMSQEAYDGFLEAMGQRVPLGRVGRP
                     EDVAWWVVRLADPEAAYASGAVLAVDGGLSVT"
     stem_loop       251..292
     CDS             complement(373..1320)
                     /NRPS_PKS="Domain: Polyketide_cyc2 (7-149). E-value:
                     2.1e-17. Score: 55.6. Matches aSDomain:
                     nrpspksdomains_dauA_Polyketide_cyc2.1"
                     /NRPS_PKS="Domain: Polyketide_cyc2 (156-311). E-value:
                     2.3e-08. Score: 26.4. Matches aSDomain:
                     nrpspksdomains_dauA_Polyketide_cyc2.2"
                     /codon_start=1
                     /gene="dauA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1128:cyclase/dehydrase (Score: 407.5; E-value:
                     6.2e-124)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC C7-C12
                     (Score: 440.9; E-value: 4.6e-135)"
                     /gene_kind="biosynthetic-additional"
                     /note="ORFF; putative"
                     /product="cyclase/dehydrase"
                     /protein_id="AAA87615.1"
                     /transl_table=11
                     /translation="MSESPLQQTEHEIHTSAAPDAVFAVLADVRSWPAVFPPSVHVEQL
                     ECADSSERIRIWATANGSLRTWTSRRELDERVRRIRFRQETSAHPVAAMGGEWIVEEAG
                     DGGTRVRLTHDFRAVDDDPETIGWIHRAVDRNSEAELASLRTALEGPDGTAPTTFEDTV
                     VVRGSAEDIYRFLHRSDLWEKRLSHVARIAVKEEEPGLQHMEMDTLTADGSVHTTASVR
                     VCFPERRLIVYKQLRTPPLLALHLGRWSVRPAGDGDGIAVTSAHTVSVARSAIPGILGA
                     GANETDAVDFVRRALGRNSLLTLEAARRYAESSA"
     gene            complement(373..7330)
                     /gene="dauA"
     aSDomain        complement(388..852)
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_dauA_Polyketide_cyc2.2"
                     /domain_subtype="Polyketide_cyc2"
                     /evalue="2.30E-08"
                     /label="dauA_Polyketide_cyc2.2"
                     /locus_tag="dauA"
                     /protein_end="311"
                     /protein_start="156"
                     /score="26.4"
                     /tool="antismash"
                     /translation="TTFEDTVVVRGSAEDIYRFLHRSDLWEKRLSHVARIAVKEEEPGL
                     QHMEMDTLTADGSVHTTASVRVCFPERRLIVYKQLRTPPLLALHLGRWSVRPAGDGDGI
                     AVTSAHTVSVARSAIPGILGAGANETDAVDFVRRALGRNSLLTLEAARRYA"
     aSModule        388..1299
                     /domains="nrpspksdomains_dauA_Polyketide_cyc2.1"
                     /domains="nrpspksdomains_dauA_Polyketide_cyc2.2"
                     /incomplete
                     /locus_tags="dauA"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        complement(874..1299)
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_dauA_Polyketide_cyc2.1"
                     /domain_subtype="Polyketide_cyc2"
                     /evalue="2.10E-17"
                     /label="dauA_Polyketide_cyc2.1"
                     /locus_tag="dauA"
                     /protein_end="149"
                     /protein_start="7"
                     /score="55.6"
                     /tool="antismash"
                     /translation="QTEHEIHTSAAPDAVFAVLADVRSWPAVFPPSVHVEQLECADSSE
                     RIRIWATANGSLRTWTSRRELDERVRRIRFRQETSAHPVAAMGGEWIVEEAGDGGTRVR
                     LTHDFRAVDDDPETIGWIHRAVDRNSEAELASLRTALE"
     regulatory      complement(1325..1330)
                     /gene="dauA"
                     /regulatory_class="ribosome_binding_site"
     gene            complement(1350..2138)
                     /gene="dauB"
     CDS             complement(1350..2138)
                     /NRPS_PKS="Domain: PKS_KR (7-133). E-value: 6e-12. Score:
                     37.7. Matches aSDomain: nrpspksdomains_dauB_PKS_KR.1"
                     /codon_start=1
                     /gene="dauB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     243.5; E-value: 4e-74)"
                     /gene_functions="biosynthetic-additional (t2pks) KR C9
                     (Score: 431.4; E-value: 2.6e-132)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative"
                     /product="polyketide reductase"
                     /protein_id="AAA87616.1"
                     /sec_met_domain="adh_short (E-value: 4.7e-38, bitscore:
                     120.2, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDAADRVALVTGGTSGIGLAVGRRKLALDGTRVFLCARDESAVT
                     GTVKELQASGLEVEGTSCDVRSTDAVDRLVRTARNPLRAIDIVVNNAGRGGGGVTAQIT
                     DDLWSDVVDTNLGGAFRVTRAVLTGGGMQEHGWGRIINIASTGGKQGVALGAPYSASKS
                     GLIGFTKAVALELARTGITVNAVCPGYVETPMAQGVRQRYAAFWGITEDDVLEKFRAKI
                     PLGRYSTSDEVAGMVHYLVSDSADSITAQAINVCGGLGSY"
     aSDomain        complement(1740..2117)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_dauB_PKS_KR.1"
                     /evalue="6.00E-12"
                     /label="dauB_PKS_KR.1"
                     /locus_tag="dauB"
                     /protein_end="133"
                     /protein_start="7"
                     /score="37.7"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VALVTGGTSGIGLAVGRRKLALDGTRVFLCARDESAVTGTVKELQ
                     ASGLEVEGTSCDVRSTDAVDRLVRTARNPLRAIDIVVNNAGRGGGGVTAQITDDLWSDV
                     VDTNLGGAFRVTRAVLTGGGMQ"
     CDS_motif       complement(2067..2111)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_dauB_0001"
                     /evalue="8.50E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="dauB"
                     /protein_end="24"
                     /protein_start="9"
                     /score="12.0"
                     /tool="antismash"
                     /translation="LVTGGTSGIGLAVGR"
     regulatory      complement(2141..2146)
                     /gene="dauA"
                     /regulatory_class="ribosome_binding_site"
     regulatory      2311..2313
                     /regulatory_class="ribosome_binding_site"
     CDS             2317..2712
                     /codon_start=1
                     /note="ORFE; putative"
                     /product="oxygenase"
                     /protein_id="AAA87617.1"
                     /transl_table=11
                     /translation="MEQRCLWPEPNDAGSGSVTFVNRFTLSGSAEDFEAAFAETAEFLC
                     RRPGFRWHVLLAPTGSGSADVRPQYVNIAVWDDEASFRAAVAHPQFPAHAAVLRALSTS
                     EPTLYRSRQIRVAPGAPAMSRPEGRTT"
     CDS             2709..3965
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (4-393). E-value:
                     3.5e-54. Score: 176.1. Matches aSDomain:
                     nrpspksdomains_AAA87618.1_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2ks"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 466; E-value:
                     1.9e-141)"
                     /gene_functions="biosynthetic-additional (t2pks) KS (Score:
                     709.6; E-value: 2.7e-216)"
                     /gene_kind="biosynthetic"
                     /note="ORFA; putative"
                     /product="polyketide synthase"
                     /protein_id="AAA87618.1"
                     /sec_met_domain="t2ks (E-value: 5.1e-217, bitscore: 710.0,
                     seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRRVVITGMGVVAPGAIGIKSFWELLLSGTTATRAITTFDATPF
                     RSRIAAECDFDPVAAGLSAEQARRLDRAGQFALVAGQEALTDSGLRIGEDSAHRVGVCV
                     GTAVGCTQKLESEYVALSAGGANWVVDPHRGAPELYDYFVPSSLAAEVAWLAGAEGPVN
                     IVSAGCTSGIDSIGYACELIREGTVDVMLAGGVDAPIAPITVACFDAIRVTSDHNDTPE
                     TLAPFSRSRNGFVLGEGGAIVVLEEAEAAVRRGARIYAEIGGYASRGNAYHMTGLRADG
                     AEMAAAITAALDEARRDPSDVDYVNAHGTATRQNDRHETSAFKRSLGDHAYRVPISSVK
                     SMIGHSLGAAGSLEVAATALAVEYGAIPPTANLHDPDPELDLDYVPLTAREKRVRHALT
                     VGSGFGGFQSAMLLSRPER"
     aSDomain        2721..3887
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAA87618.1_PKS_KS.1"
                     /domain_subtype="Iterative-KS"
                     /evalue="3.50E-54"
                     /label="AAA87618.1_PKS_KS.1"
                     /locus_tag="AAA87618.1"
                     /protein_end="393"
                     /protein_start="4"
                     /score="176.1"
                     /tool="antismash"
                     /translation="VVITGMGVVAPGAIGIKSFWELLLSGTTATRAITTFDATPFRSRI
                     AAECDFDPVAAGLSAEQARRLDRAGQFALVAGQEALTDSGLRIGEDSAHRVGVCVGTAV
                     GCTQKLESEYVALSAGGANWVVDPHRGAPELYDYFVPSSLAAEVAWLAGAEGPVNIVSA
                     GCTSGIDSIGYACELIREGTVDVMLAGGVDAPIAPITVACFDAIRVTSDHNDTPETLAP
                     FSRSRNGFVLGEGGAIVVLEEAEAAVRRGARIYAEIGGYASRGNAYHMTGLRADGAEMA
                     AAITAALDEARRDPSDVDYVNAHGTATRQNDRHETSAFKRSLGDHAYRVPISSVKSMIG
                     HSLGAAGSLEVAATALAVEYGAIPPTANLHDPDPELDLDYVPLTAREKR"
     regulatory      4041..4043
                     /regulatory_class="ribosome_binding_site"
     CDS             4052..5248
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (61-396). E-value:
                     7.6e-22. Score: 69.6. Matches aSDomain:
                     nrpspksdomains_AAA87619.1_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2clf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093:Beta-ketoacyl synthase (Score: 582.3; E-value:
                     6.9e-177)"
                     /gene_functions="biosynthetic-additional (t2pks) CLF 8|9
                     (Score: 616.0; E-value: 5.9e-188)"
                     /gene_kind="biosynthetic"
                     /note="ORFB; putative"
                     /product="polyketide synthase"
                     /protein_id="AAA87619.1"
                     /sec_met_domain="t2clf (E-value: 7.5e-175, bitscore: 570.6,
                     seeds: 26, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVTGLGIVAPNGLGVGAIWDAVLNGRNGIGPLRRFADDGRLGRLA
                     GEVSDFVPEDHLPKRLLVQTDPMTQMTALAAAEWALREAGCAPSSPLEAGVITASASGG
                     FASGQRELQNLWSKGPAHVSAYMSFAWFYAVNTGQIAIRHDLRGPVGVVVAEQAGGLDA
                     LAHARRKVRGGAELIVSGAMDSSLCPYGMAAQVRSGRLSGSDDPTAGYLPFDRRAAGHV
                     PGEGGAILAVEDAERVAERGGKVYGSIAGTASFDPPPGSGRPSALARAVETALADAGLD
                     RSDIAVVFADGAAVGELDVAEAEALASVFGPHRVPVTVPKTLTGRLYSGAGPLDVATGL
                     LALRDEVVPATGHVHPDPDLPLDVVTGRPRAMADARAALVVARGHGGFNSALVVRGAA"
     aSDomain        4235..5239
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAA87619.1_PKS_KS.1"
                     /domain_subtype="Iterative-KS"
                     /evalue="7.60E-22"
                     /label="AAA87619.1_PKS_KS.1"
                     /locus_tag="AAA87619.1"
                     /protein_end="396"
                     /protein_start="61"
                     /score="69.6"
                     /tool="antismash"
                     /translation="LVQTDPMTQMTALAAAEWALREAGCAPSSPLEAGVITASASGGFA
                     SGQRELQNLWSKGPAHVSAYMSFAWFYAVNTGQIAIRHDLRGPVGVVVAEQAGGLDALA
                     HARRKVRGGAELIVSGAMDSSLCPYGMAAQVRSGRLSGSDDPTAGYLPFDRRAAGHVPG
                     EGGAILAVEDAERVAERGGKVYGSIAGTASFDPPPGSGRPSALARAVETALADAGLDRS
                     DIAVVFADGAAVGELDVAEAEALASVFGPHRVPVTVPKTLTGRLYSGAGPLDVATGLLA
                     LRDEVVPATGHVHPDPDLPLDVVTGRPRAMADARAALVVARGHGGFNSALVVRG"
     CDS             5245..6303
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     PKS-like: ksIII"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084:3-oxoacyl-(acyl carrier protein) synthase III
                     (Score: 120.3; E-value: 1.5e-36)"
                     /gene_functions="biosynthetic-additional (t2pks) KSIII
                     (Score: 465.1; E-value: 3.1e-142)"
                     /gene_functions="biosynthetic-additional (t2pks) KSIII
                     WP_100109100.1_Doxorubicin_propionyl-CoA (Score: 591.0;
                     E-value: 0)"
                     /gene_kind="biosynthetic"
                     /note="ORFC; beta-keto acyl synthase III homologue;
                     putative"
                     /protein_id="AAA87620.1"
                     /sec_met_domain="ksIII (E-value: 2.4e-92, bitscore: 298.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTLPGAPGDLYVAGCGVWLPPPVTTDQALAAGHCDRRLASSTRM
                     LSVAVADKETPAEMAASAARTAVDRSGVPPARIVLVLHASLYFQGHHLWAPASYVQRVA
                     LGNRCPAMEVRQVSNGGMAALELARAYLLAAPDRTAALVTTGDRMSPPGFDRWNRPRHV
                     YADGGTALVLSRQGGFARLRSLVTVSEPVLEGMHRGGHPFGPPSAEEQRTVDLDAHSGR
                     TWPRREARSASPRVSAGQEEALAGALKAAGVGLDDISRVVLPHMGWRRLSASYFGKWPV
                     PPERTTWEFGRRTGHLGGGDPIAGFDHLVGSGRLAPGELCLLVSVGAGFSWSCAVVELL
                     DARRWAAAPAAR"
     stem_loop       6277..6297
     regulatory      6303..6307
                     /regulatory_class="ribosome_binding_site"
     CDS             6314..7330
                     /NRPS_PKS="Domain: PKS_AT (8-297). E-value: 3.3e-66. Score:
                     215.5. Matches aSDomain:
                     nrpspksdomains_AAA87621.1_PKS_AT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021:malonyl CoA-acyl carrier protein transacylase
                     (Score: 245.1; E-value: 2.7e-74)"
                     /gene_functions="biosynthetic-additional (t2pks) AT (Score:
                     429.5; E-value: 1.9e-131)"
                     /gene_functions="biosynthetic-additional (t2pks) AT
                     AAA87621.1_Daunomycin_propionyl-CoA (Score: 659.0; E-value:
                     0)"
                     /gene_kind="biosynthetic-additional"
                     /note="ORFD; putative"
                     /product="acyltransferase"
                     /protein_id="AAA87621.1"
                     /sec_met_domain="PKS_AT (E-value: 5.3e-67, bitscore: 215.5,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDVPAILLMFPGQGSQHARMAAGLYGSEPVFTAAMDEVLAAYDAA
                     GDCEGQRLRADWLSDRPAVPLDHVTRSQPLLFAVDYALGRMVLATTGRPWALLGHSVGE
                     MAGAALAGVFTLRDAACLLLDRVRRLAQAPPGGMLAVAAGEDVLGPYLVEGEVAVAAVN
                     SPRQTILAGPVAPLAAVARALREAGLTCREVPSLTAFHSPVLAPYTTGAAERFASTRVA
                     APSIPLRSGYRPGAVTGRLAADPSYWAGHPVETVRFWPALDAMLRSRTGVVCLETGPGQ
                     GLSTVARRHPTVRSGRNAVVSLLPPRAGTAEMDRTSVAAALTAVRERGAVLSGRPGR"
     CDS_motif       6335..6388
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AAA87621.1_0001"
                     /evalue="1.10E-05"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="AAA87621.1"
                     /protein_end="25"
                     /protein_start="7"
                     /score="17.8"
                     /tool="antismash"
                     /translation="LMFPGQGSQHARMAAGLY"
     aSDomain        6338..7204
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAA87621.1_PKS_AT.1"
                     /evalue="3.30E-66"
                     /label="AAA87621.1_PKS_AT.1"
                     /locus_tag="AAA87621.1"
                     /protein_end="297"
                     /protein_start="8"
                     /score="215.5"
                     /specificity="consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="MFPGQGSQHARMAAGLYGSEPVFTAAMDEVLAAYDAAGDCEGQRL
                     RADWLSDRPAVPLDHVTRSQPLLFAVDYALGRMVLATTGRPWALLGHSVGEMAGAALAG
                     VFTLRDAACLLLDRVRRLAQAPPGGMLAVAAGEDVLGPYLVEGEVAVAAVNSPRQTILA
                     GPVAPLAAVARALREAGLTCREVPSLTAFHSPVLAPYTTGAAERFASTRVAAPSIPLRS
                     GYRPGAVTGRLAADPSYWAGHPVETVRFWPALDAMLRSRTGVVCLETGPGQGLSTVARR
                     HPTVRSGR"
     CDS_motif       6530..6562
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AAA87621.1_0002"
                     /evalue="4.30E-03"
                     /label="PKSI-AT-M_m2"
                     /locus_tag="AAA87621.1"
                     /protein_end="83"
                     /protein_start="72"
                     /score="10.2"
                     /tool="antismash"
                     /translation="QPLLFAVDYAL"
     CDS_motif       6590..6691
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AAA87621.1_0003"
                     /evalue="3.30E-13"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="AAA87621.1"
                     /protein_end="126"
                     /protein_start="92"
                     /score="41.6"
                     /tool="antismash"
                     /translation="RPWALLGHSVGEMAGAALAGVFTLRDAACLLLDR"
     CDS_motif       6791..6829
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AAA87621.1_0004"
                     /evalue="6.40E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="AAA87621.1"
                     /protein_end="172"
                     /protein_start="159"
                     /score="13.0"
                     /tool="antismash"
                     /translation="AAVNSPRQTILAG"
     CDS_motif       7052..7087
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AAA87621.1_0005"
                     /evalue="4.30E-03"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="AAA87621.1"
                     /protein_end="258"
                     /protein_start="246"
                     /score="10.7"
                     /tool="antismash"
                     /translation="YWAGHPVETVRF"
     stem_loop       7458..7502
     gene            complement(7505..8089)
                     /gene="dauC"
     CDS             complement(7505..8089)
                     /codon_start=1
                     /gene="dauC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 96.1; E-value: 4e-29)"
                     /gene_functions="biosynthetic-additional (t2pks) MET
                     carboxy_O (Score: 264.4; E-value: 1.8e-81)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative"
                     /product="aklanonic acid methyltransferase"
                     /protein_id="AAA87622.1"
                     /sec_met_domain="PCMT (E-value: 1e-09, bitscore: 27.7,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EFFTPMGRRLVDISEPVTGERVLDIGCGRGACLFPAAEKVGSQGC
                     VHGIDIAPGMIEEARKEATERGLRNISLMVMDAETPGFPARSFDLVMGSYSVIFLPDAV
                     GALARYADILDHGGRIAFTSPVFRAGTFPFLPPEFTPLIPQALLEHLPEQWRPEALVRR
                     FNSWLERAEDLVRTLEGCGYARLRQSTSRCG"
ORIGIN
        1 gaattcgcgt gatcggggtc gccccgggag tgatcgagac gggcgcggga gtccgggccg
       61 ggatgtccca ggaggcgtac gacgggttcc tggaagccat gggacaacgc gtcccgctcg
      121 gccgggtggg ccggcccgag gacgtcgcgt ggtgggtggt gcggctcgcc gatcccgaag
      181 ccgcgtacgc cagcggtgcc gtgctcgccg tggacggggg gctgtcggtg acctgagagg
      241 cccggtcgga cccgcacagg tcgccctcgc tcgccggagg gcgacctgcg gggtggtgtc
      301 ctgccgggga cacggaggcc cccgccccga gggggtcggg acgggggttc cgtacgggtc
      361 gggacgctcg tctcaggcgc tggactcggc gtaccgtctg gccgcttcca gggtcagcag
      421 actgttgcgg cccagggccc ggcggacgaa gtccacggcg tccgtctcat tcgcccccgc
      481 acctaggatg ccggggatcg ccgagcgggc cacggacacg gtgtgggccg acgtgacggc
      541 gatcccgtcc ccgtcgccgg cgggacggac cgaccagcgg ccgagatgga gcgccagcag
      601 cggcggcgtg cgcagttgct tgtagacgat gaggcggcgt tcggggaagc acacgcgcac
      661 cgacgcggtg gtgtgtaccg agccgtccgc ggtgagggtg tccatctcca tgtgctgcag
      721 acccggttcc tcctccttga ccgcgatccg cgcgacgtgg gagagccgct tctcccagag
      781 gtcgctgcga tggaggaagc ggtagatgtc ctcggcactg ccacggacga cgacggtgtc
      841 ctcgaaggtc gtcggagcgg tgccgtcggg tccttccagc gcggtgcgca gcgacgccag
      901 ttcggcttcg ctgttgcggt ccacggcccg atggatccag ccgatcgtct ccgggtcgtc
      961 gtccacggcc cggaagtcgt gcgtcagccg cacgcgggtg ccgccgtcac cggcttcctc
     1021 gacgatccat tcgccgccca tggctgcgac ggggtgggcg gacgtctcct gccggaaacg
     1081 gatgcgccgg acccgctcgt cgagctcgcg ccgcgacgtc caggtacgaa gcgagccgtt
     1141 ggcagtggcc cagatgcgga tgcgttcgct ggagtccgcg cactccaact gttcgacgtg
     1201 gacactgggc gggaacacgg cgggccagga ccgcacgtcg gccagcacgg cgaacacggc
     1261 gtccggcgcg gccgaggtat ggatctcgtg ctcggtctgc tgaagcggcg actcggacat
     1321 ctgccctcct gagctcgggt cgtggacggt cagtaactgc cgaggccacc acagacattg
     1381 atggcctggg ccgtgatcga gtcggcgctg tcggagacca ggtagtgcac cattccggcc
     1441 acctcgtcgg acgtcgagta gcgtccgagc gggatcttcg cccggaactt ctccaggacg
     1501 tcgtcctcgg tgatccccca gaaggcggcg tatcgctgcc gcacgccttg ggccatgggc
     1561 gtctcgacgt accccgggca gacggcgttg accgtgatgc ccgtcctggc cagctccagt
     1621 gcgacagcct tcgtgaagcc gatcaggccg ctcttggagg cggagtaggg agcgccgagg
     1681 gccactccct gcttgccgcc ggtagaggcg atgttgatga tccggcccca gccgtgctcc
     1741 tgcatgccgc cgccggtgag gacggcgcgg gtgacccgga aggccccgcc gaggttggtg
     1801 tcgacgacat cggaccacag gtcgtccgtg atctgggcgg tcactcctcc accaccgcgg
     1861 ccggcgttgt tgacgacgat gtcgatcgcc cgaagcgggt tccgggccgt tcggaccagc
     1921 cggtcgacag cgtcggtgct ccgcacgtcg caggacgtgc cctccacctc cagtcccgat
     1981 gcctgaagtt ccttcacggt gccggtgacg gcgctctcgt cacgagcgca caggaacacc
     2041 cgggtcccgt ccagggcgag cttgcgtcgt cctaccgcga gaccgatgcc gctggttccc
     2101 ccggtgacaa gtgcgacccg gtcggctgcg tcggacatga tcctcccttc gacggagggc
     2161 ccaccctcac acctcgcgct cgagccgcgc ttgacgaccc ggcttccaga ggaggtcgag
     2221 cggcgccgca gaacctcgga gtccggcgtg accgcacctg cggacgcggc cgcaccgtcg
     2281 caccgacgtc caccccacac ctggaccaac ggacccgtgg agcagcgatg cctctggcca
     2341 gaaccgaacg acgccggctc cgggtcggtg accttcgtca accgattcac cctgagcgga
     2401 tcagccgagg acttcgaggc ggcgttcgcc gaaacggccg agttcctgtg tcgccgaccc
     2461 ggcttccgct ggcacgtcct gctggcgccg accggctccg gctcggccga cgtccgcccg
     2521 cagtacgtga acatcgcggt ctgggacgac gaggcctcct tccgtgccgc tgtggcgcac
     2581 ccgcagttcc cggcccacgc ggccgtcctg cgggcgctga gcaccagcga accgaccctg
     2641 taccggtccc gtcagatccg tgtcgcgccg ggcgccccgg ccatgagcag gcccgaaggg
     2701 cggacgacgt gaaccgacgg gtcgtcatca ccgggatggg tgtggtggcc ccgggagcca
     2761 tcggcatcaa gtcgttctgg gagctgctgc tgtccgggac caccgccacc cgggccatca
     2821 ccacgttcga cgccacgccc ttccgttccc ggatcgccgc cgagtgcgac ttcgatccgg
     2881 tcgcggcagg gctgtccgcg gagcaggccc ggcgcctcga ccgggccggg cagttcgcgc
     2941 tggtcgccgg tcaggaggcg ctcactgaca gcgggctgcg catcggggag gactccgccc
     3001 accgggtcgg ggtgtgcgtg ggtaccgctg tcggctgcac gcagaaactg gagtccgagt
     3061 acgtggctct cagcgcgggc ggtgcgaact gggtcgtgga cccccaccgg ggggcgcccg
     3121 agctgtacga ctacttcgtg ccgagttccc tcgccgccga agtggcttgg ctcgccgggg
     3181 cggaagggcc cgtgaacatc gtctcggccg ggtgcacctc ggggatcgac tccatcgggt
     3241 acgcctgcga actcatccgc gaggggacgg tggacgtcat gctcgcggga ggcgtggacg
     3301 cccccatcgc accgatcact gtggcctgct tcgacgccat cagggtgacc tccgaccaca
     3361 acgacacacc cgagacgctc gcgcccttca gccgcagccg caacggcttc gtgctgggcg
     3421 agggtggtgc gatcgtggtt ctggaagagg cagaggcggc cgtgcgcagg ggcgcacgaa
     3481 tctacgccga gatcggcggg tacgcctcac gcggcaacgc ataccacatg acggggctgc
     3541 gcgcagacgg cgccgagatg gctgcggcga ttaccgcggc cctcgacgaa gcccgccgcg
     3601 acccctcgga cgtggactat gtcaacgccc acggaacagc gacgcgacag aacgaccggc
     3661 acgaaacctc cgcgttcaag cgctcgctgg gggatcacgc gtaccgggtt cccatcagct
     3721 ccgtgaagtc gatgatcggt cactccctgg gggccgccgg ctcgctcgaa gtggcggcga
     3781 ccgcactggc ggtggagtac ggggcgatcc cgccgaccgc gaatctgcac gacccggatc
     3841 ccgaactcga cctggactac gtgccgttga cggcgcgaga aaagcgggtg cggcatgcgc
     3901 tgacggtggg cagcggcttc ggcggcttcc agagcgccat gctgttgagc cgtcccgaga
     3961 ggtgagcgcc atgacacgtc cagctgaagg ggccgcatcc tcgcagatcc gcgcatcatc
     4021 ctcgggccgg cggtgcctca agggccgcgc ggtggtgacg ggtctcggga tcgtcgcccc
     4081 caacgggctc ggtgtgggcg cgatctggga cgccgtcctg aacggccgca acggcatcgg
     4141 accgctgagg cggttcgccg acgacggtcg cctggggcgg ctggccggtg aggtgagcga
     4201 cttcgtcccc gaggaccatc tgccgaagcg gctgctggtg cagacagatc cgatgaccca
     4261 gatgacggcg ctggccgccg ccgagtgggc cctgcgggag gccggctgcg cgccgtcgtc
     4321 gccgctggag gcgggggtga tcacggccag cgcttccggc ggcttcgcgt ccggccagcg
     4381 ggaattgcag aacctgtgga gcaaggggcc ggcccacgtc agcgcctaca tgtcgttcgc
     4441 ctggttctac gccgtcaaca ccgggcagat cgccatccgc cacgacctgc gtggtccggt
     4501 cggagtggtg gtggccgagc aggcgggcgg ccttgacgcc ctcgcccacg cccgccgcaa
     4561 ggtccggggc ggagccgagc tgatcgtctc gggcgccatg gactcgtcat tgtgcccgta
     4621 cggtatggcg gcccaggtga ggtccggtcg gctgagcggc tcggacgacc cgacggccgg
     4681 gtatctgccg ttcgaccggc gagcggccgg acacgtgccc ggtgaggggg gcgcgatcct
     4741 cgccgtcgag gacgcggagc gcgtcgccga acggggcggg aaggtgtacg ggtcgatcgc
     4801 cggtacggcg agcttcgacc cgccccccgg ctccggccgc ccctccgccc tcgcccgtgc
     4861 cgtcgagacg gcgctggccg acgcggggct ggaccgatcc gacatagccg tggtgttcgc
     4921 cgacggcgcg gccgtcggcg aactggacgt ggccgaggcg gaggccctgg cgtcggtctt
     4981 cggaccgcac cgggtcccgg tgaccgtccc caagacgctc accggccgac tgtactcggg
     5041 agccgggccg ctggacgtgg ccaccggact gctggcgctg cgggacgagg tggtcccggc
     5101 gaccgggcac gtccacccgg accccgatct gccgctggac gtggtgaccg ggcggccccg
     5161 ggcgatggcc gacgccaggg cggctctcgt cgtcgcccgg ggccacgggg gtttcaacag
     5221 cgctctggtc gtccgggggg cggcgtgagc acgctgccgg gagcacctgg ggacctctac
     5281 gtggccggtt gcggtgtctg gctgcccccg ccggtgacca cggatcaggc cctggccgcc
     5341 ggtcactgcg accgcaggct cgcctcctcg acgcggatgc tgtcggtggc ggtcgccgac
     5401 aaggagacac cggcggagat ggcggcctcg gcggcccgga ccgctgtgga ccgctccggc
     5461 gtccccccgg cccgcatcgt cctcgtcctg cacgccagcc tgtacttcca ggggcaccat
     5521 ctgtgggctc ccgcctccta cgtccagcgg gtggccctcg ggaaccgttg ccccgccatg
     5581 gaggtgcggc aggtgtccaa cggcggcatg gccgccctgg agctcgcccg cgcgtacctg
     5641 ctcgccgccc cggaccggac ggccgctctc gtcacgacgg gcgaccggat gagtccgccg
     5701 ggcttcgacc gctggaacag accccggcac gtgtacgccg acggcggcac cgccctcgta
     5761 ctgtcccggc agggaggctt cgcccggctg cgtagcctgg tgacggtctc cgaaccggtg
     5821 ctggagggca tgcaccgggg cggacacccg ttcggcccgc cgtccgcgga ggagcagcgg
     5881 accgtcgacc tggacgcgca cagcgggcgt acgtggccga ggcgggaggc tcgttcagcg
     5941 tcgccgcggg tctcggcggg ccaggaggag gctctggccg gcgcgctgaa ggccgccggg
     6001 gtcggactcg acgacatcag ccgggtcgtg ctgccgcaca tgggctggcg caggctgagc
     6061 gcctcctact tcggaaagtg gccggttccg cccgagcgga ccacgtggga gttcgggcga
     6121 cggaccggcc atctgggcgg cggcgacccg atcgccgggt tcgaccacct cgtgggctcc
     6181 ggccgcctcg ccccggggga attgtgtctg ctggtcagcg tcggtgccgg ctttagctgg
     6241 tcgtgcgccg tcgtggagtt gctggacgcc cgtcggtggg cggccgcccc ggccgcccgg
     6301 tgaggagacc gtcatggacg tgcccgcgat tctcctgatg ttcccggggc aggggtccca
     6361 gcacgcgagg atggcagccg ggctgtacgg atccgaaccg gtcttcaccg ctgccatgga
     6421 cgaggtcctc gccgcgtacg atgccgccgg cgactgtgag ggccagcggc tgcgtgccga
     6481 ctggctgagc gaccgtccag ccgtgccgct ggaccacgtc acccggtccc agccgctgtt
     6541 gttcgcggtg gactacgccc tcggcaggat ggtactggcc acgacgggcc ggccgtgggc
     6601 cctgctcggg cacagtgtcg gcgagatggc gggggccgcg ctcgccgggg tgttcacgct
     6661 gcgcgacgcg gcctgcctcc tgctggaccg ggtgcgacgg ctggcccagg caccgcccgg
     6721 ggggatgctg gcggtcgccg cgggcgagga cgtgctgggc ccgtatctcg tggaggggga
     6781 ggtggcggtc gcggccgtca actcgccccg ccagaccatc ctcgccggcc ccgtcgcacc
     6841 gctcgccgcg gtcgcccggg cactgcggga ggcggggctc acatgccgtg aggtgccctc
     6901 gctgaccgca ttccacagtc cggtgctggc cccgtacacg acgggggcgg ccgagcggtt
     6961 cgcatcgacg cgcgtggcgg cgccgtccat ccctctccgc tccgggtacc ggccgggcgc
     7021 ggtgaccggg cgactggccg ccgacccgtc ctactgggcc ggacatcccg tggagacggt
     7081 ccgcttctgg ccggcactgg acgcgatgct ccgctcccgc acgggcgtcg tctgcctgga
     7141 gaccgggccg gggcaggggc tcagcaccgt cgcccgccgc catccgacgg tgcggtcggg
     7201 gaggaacgcg gtggtgtcgc tgctgccgcc gcgggccggc accgccgaga tggaccggac
     7261 gtccgtggcg gcggcgctga ccgcagtgcg cgagcggggc gctgtgctca gtgggcgacc
     7321 cgggcggtga cgaagcggac aggcacgtcg atggcgaggg ccccggtggc gtcgctcagc
     7381 ttgtcgagtc cctcgacgag ccgtgcgcgc agttccgttc gctgcggctg cgggaggttc
     7441 tgccacagca gacgcatgcc ctgggtgtgg gaccagtcca cccacgtctc gctggacggg
     7501 gccgtcatcc gcaccggctc gtcgactgac ggagacgagc gtaaccgcag ccttcaaggg
     7561 tacggacaag atcctcggct cgctccagcc agctgttgaa ccgccggacc agcgcctcgg
     7621 gccgccactg ctcggggagg tgctccagca atgcctgggg gatgagcggg gtgaactccg
     7681 gcggcaggaa cgggaacgtg ccggcacgga agaccgggct ggtgaacgcg atcctgccgc
     7741 cgtggtccag gatgtcggcg tagcgcgcga gggcccccac ggcatccggc aggaaaatga
     7801 cgctgtagct ccccatgacc aggtcgaagg aacgcgccgg aaatccgggt gtctccgcat
     7861 ccatcaccat gaggctgata ttccgcagac cgcgttcggt ggcttccttg cgagcttcct
     7921 cgatcattcc gggagcgatg tcgataccgt ggacgcaccc ctgggagccg accttttccg
     7981 cagccgggaa aaggcatgcg ccgcgcccgc agccgatgtc gagcactcgt tcacccgtga
     8041 ccggctccga gatgtccacc aggcggcgac ccatgggcgt gaagaattc
//
