BGC0000218 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000218 (Streptomyces peucetius)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	PKS-like,T2PKS	1	8,737	High	doxorubicin	PKS

BGC0000218 - Region 1 - PKS-like,T2PKS

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Location: 1 - 8,737 nt. (total: 8,737 nt) Show pHMM detection rules used

Region on contig edge.

PKS-like: (t2pks2 or ksIII)
T2PKS: (t2ks and t2clf)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 8,738
Label		BGC0000218

Tailoring functions

Oxidoreductases: dehydrogenase

dnrH: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

dpsE: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase methyltransferase

AAA65210.1: methyltransferase

smcogs: SMCOG1089: methyltransferase

dpsD: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Lyases: cyclase

dpsF: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

Ligases: synthase

dpsA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

dpsB: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

dpsC: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000218.5	2.00	PKS	doxorubicin	Streptomyces peucetius
BGC0000217.5	1.40	PKS	daunorubicin	Streptomyces sp.
BGC0000231.5	0.62	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001284.4	0.55	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001160.5	0.54	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001254.3	0.53	PKS	ACT-Toxin II	Alternaria alternata
BGC0001244.3	0.52	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.52	PKS	asperlactone	Aspergillus ochraceus
BGC0000192.3	0.51	PKS	aclacinomycin	Streptomyces galilaeus
BGC0000234.5	0.51	PKS	jadomycin	Streptomyces venezuelae ATCC 10712

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000218.5	1.00	PKS	doxorubicin	Streptomyces peucetius
BGC0000217.5	0.89	PKS	daunorubicin	Streptomyces sp.
BGC0000231.5	0.64	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001284.4	0.61	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001160.5	0.60	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001254.3	0.60	PKS	ACT-Toxin II	Alternaria alternata
BGC0001244.3	0.60	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.59	PKS	asperlactone	Aspergillus ochraceus
BGC0000192.3	0.59	PKS	aclacinomycin	Streptomyces galilaeus
BGC0000234.5	0.59	PKS	jadomycin	Streptomyces venezuelae ATCC 10712

Similar known clusters from MIBiG 4.0

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

dpsD: mal

PKS_AT (8..290): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 52.3
Methylmalonyl-CoA: 33.7
Methoxymalonyl-CoA: 31.8
Propionyl-CoA: 26.3
inactive: 20.1
2-Methylbutyryl-CoA: 9.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 8737
Starter unit(s):: propionyl-CoA (Score: 1354.0; E-value: 0)
Malonyl elongations:: 8|9 (Score: 652.2; E-value: 5.9e-199)
Product class(es):: angucycline
anthracycline
aureolic acid
tetracycline
Product molecular weight(s):: propionyl-CoA_8: 396 Da
propionyl-CoA_9: 438 Da

AAA65210.1: MET carboxy_O (Score: 374.6; E-value: 4.9e-115)
dnrH: KR C17 (Score: 380.6; E-value: 7.3e-117)
dpsA: KS (Score: 728.0; E-value: 7.2e-222)
dpsB: CLF 8|9 (Score: 652.2; E-value: 5.9e-199)
dpsC: KSIII (Score: 519.2; E-value: 1.1e-158); KSIII WP_100109100.1_Doxorubicin_propionyl-CoA (Score: 700.0; E-value: 0.0)
dpsD: AT (Score: 423.5; E-value: 1.3e-129); AT WP_100109099.1_Doxorubicin_propionyl-CoA (Score: 654.0; E-value: 0.0)
dpsE: KR C9 (Score: 453.7; E-value: 4e-139)
dpsF: CYC C7-C12 (Score: 444.0; E-value: 5.1e-136)