BGC0000220 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000220 (Streptomyces maritimus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T2PKS	1	22,511	High	enterocin	PKS:Type II

BGC0000220 - Region 1 - T2PKS

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Location: 1 - 22,511 nt. (total: 22,511 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 22,512
Label		BGC0000220

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxygenase

encD: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

encM: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

encR: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acetyltransferase acylase

encJ: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

encL: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Lyases: hydratase

encI: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase synthase

encA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

encB: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

encH: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

encN: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000220.4	0.97	PKS	enterocin	Streptomyces maritimus
BGC0002366.2	0.92	PKS	enterocin, neoenterocin A, neoenterocin B	Streptomyces sp. SCSIO 11863
BGC0000231.5	0.69	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001164.3	0.62	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000272.3	0.61	PKS	spore pigment	Streptomyces collinus
BGC0001723.5	0.58	PKS	oviedomycin	Streptomyces antibioticus
BGC0000221.5	0.58	PKS	erdacin	uncultured soil bacterium V167
BGC0000234.5	0.58	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002286.3	0.58	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.58	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000220.4	0.97	PKS	enterocin	Streptomyces maritimus
BGC0002366.2	0.92	PKS	enterocin, neoenterocin A, neoenterocin B	Streptomyces sp. SCSIO 11863
BGC0000231.5	0.69	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001164.3	0.62	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000272.3	0.61	PKS	spore pigment	Streptomyces collinus
BGC0001723.5	0.58	PKS	oviedomycin	Streptomyces antibioticus
BGC0000221.5	0.58	PKS	erdacin	uncultured soil bacterium V167
BGC0000234.5	0.58	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002286.3	0.58	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.58	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

encH: X

AMP-binding (35..422): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: MDWYLSVLDK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

encL: mal

PKS_AT (18..299): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 56.3
Methoxymalonyl-CoA: 34.1
Methylmalonyl-CoA: 29.2
Propionyl-CoA: 21.8
Benzoyl-CoA: 18.7
inactive: 14.1
2-Methylbutyryl-CoA: 12.8
fatty_acid: 6.1
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

encN: X

AMP-binding (20..415): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AYGFVAGHIK
Nearest Stachelhaus code(s):: pNH2-Bz AYNFIAGSTK (47% 8Å match); diOH-Bz AANFVSGAIK (24% 8Å match)
Stachelhaus code match:: 60% (weak)

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 22511
Starter unit(s):: benzoyl-CoA (Score: 2839.0; E-value: 0)
Malonyl elongations:: 7 (Score: 734.8; E-value: 5e-224)
Product molecular weight(s):: benzoyl-CoA_7: 416 Da

encA: KS (Score: 719.9; E-value: 2e-219)
encB: CLF 7 (Score: 734.8; E-value: 5e-224)
encC: ACP (Score: 89.9; E-value: 1.8e-28)
encD: KR C9 (Score: 412.3; E-value: 1.7e-126)
encG: CYC C1-C18|C2-C19 (Score: 175.5; E-value: 1.1e-54)
encH: LIG (Score: 677.9; E-value: 1.7e-206); LIG AAF81723.1_Enterocin_benzoyl-CoA (Score: 1093.0; E-value: 0.0)
encK: MET (Score: 89.7; E-value: 4.5e-28)
encL: AT (Score: 420.4; E-value: 1.2e-128); AT AAF81731.1_Enterocin_benzoyl-CoA (Score: 679.0; E-value: 0.0)
encM: OXY (Score: 36.4; E-value: 7.4e-12)
encN: LIG (Score: 680.2; E-value: 3.7e-207); LIG AAF81733.1_Enterocin_benzoyl-CoA (Score: 1067.0; E-value: 0.0)