BGC0000224 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000224 (Streptomyces griseus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T2PKS	1	33,064	High	fredericamycin A	PKS:Type II

BGC0000224 - Region 1 - T2PKS

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Location: 1 - 33,064 nt. (total: 33,064 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 33,065
Label		BGC0000224

Tailoring functions

Oxidoreductases: dehydrogenase

AAQ08912.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

AAQ08926.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase methyltransferase transferase

AAQ08909.1: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

AAQ08910.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

AAQ08925.1: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Hydrolases: hydrolase

AAQ08933.1: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Lyases: cyclase

AAQ08914.1: Polyketide synthesis cyclase

smcogs: SMCOG1225: Polyketide synthesis cyclase

AAQ08919.1: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

Ligases: synthase

AAQ08916.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AAQ08917.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

AAQ08929.1: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000224.4	1.00	PKS	fredericamycin A	Streptomyces griseus
BGC0000190.5	0.82	PKS	A-74528	Streptomyces sp. SANK 61196
BGC0000231.5	0.64	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001160.5	0.62	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.62	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000275.4	0.61	PKS	tetracenomycin C	Streptomyces glaucescens
BGC0000234.5	0.60	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001723.5	0.60	PKS	oviedomycin	Streptomyces antibioticus
BGC0000272.3	0.60	PKS	spore pigment	Streptomyces collinus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000224.4	1.00	PKS	fredericamycin A	Streptomyces griseus
BGC0000190.5	0.82	PKS	A-74528	Streptomyces sp. SANK 61196
BGC0000231.5	0.64	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001160.5	0.62	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.62	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000275.4	0.61	PKS	tetracenomycin C	Streptomyces glaucescens
BGC0000234.5	0.60	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001723.5	0.60	PKS	oviedomycin	Streptomyces antibioticus
BGC0000272.3	0.60	PKS	spore pigment	Streptomyces collinus

Similar known clusters from MIBiG 4.0

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

AAQ08910.1: mal

PKS_AT (11..294): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 44.7
Methylmalonyl-CoA: 39.8
Methoxymalonyl-CoA: 39.4
fatty_acid: 18.1
inactive: 17.2
Ethylmalonyl-CoA: 14.8
Propionyl-CoA: 10.9
Benzoyl-CoA: 9.8
3-Methylbutyryl-CoA: 7.5
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
2-Methylbutyryl-CoA: -0.8

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 33064
Starter unit(s):: hexadienyl-CoA (Score: 1322.0; E-value: 0)
malonamyl-CoA (Score: 561.0; E-value: 0)
Malonyl elongations:: 11|12 (Score: 714.4; E-value: 7.3e-218)
Product class(es):: pentangular polyphenol
Product molecular weight(s):: hexadienyl-CoA_11: 512 Da
hexadienyl-CoA_12: 554 Da
malonamyl-CoA_11: 503 Da
malonamyl-CoA_12: 545 Da

AAQ08910.1: AT (Score: 422.9; E-value: 2.1e-129); AT AAQ08910.1_Fredericamycin_hexadienyl-CoA (Score: 664.0; E-value: 0.0)
AAQ08912.1: KR (Score: 232.0; E-value: 2.1e-71)
AAQ08914.1: CYC C4-C21/C2-C23|C2-C19 (Score: 173.1; E-value: 3e-54)
AAQ08915.1: CYC C5-C18 (Score: 212.3; E-value: 4.3e-66)
AAQ08916.1: KS (Score: 732.9; E-value: 2.3e-223)
AAQ08917.1: CLF 11|12 (Score: 714.4; E-value: 7.3e-218)
AAQ08918.1: ACP (Score: 99.5; E-value: 1.9e-31)
AAQ08919.1: CYC C9-C14 (Score: 251.1; E-value: 7.1e-78)
AAQ08925.1: MET C11O (Score: 552.4; E-value: 7.6e-169)
AAQ08926.1: KR C19 (Score: 431.3; E-value: 2.4e-132)
AAQ08929.1: KSIII (Score: 429.9; E-value: 1.5e-131); KSIII AAQ08929.1_Fredericamycin_hexadienyl-CoA (Score: 658.0; E-value: 0.0)
AAQ08933.1: AMID (Score: 779.5; E-value: 4.4e-237); AMID ELQ83270.1_Oxytetracycline_malonamyl-CoA (Score: 561.0; E-value: 0.0)