BGC0000225 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000225 (Streptomyces roseofulvus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T2PKS	1	25,306	High	frenolicin B	PKS

BGC0000225 - Region 1 - T2PKS

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Location: 1 - 25,306 nt. (total: 25,306 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 25,307
Label		BGC0000225

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

frnH: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

frnP: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

frnT: acyl-CoA dehydrogenase type 2

smcogs: SMCOG1191: acyl-CoA dehydrogenase type 2

Transferases: acylase

frnK: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Lyases: cyclase

frnQ: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

Ligases: synthase

frnI: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

frnL: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

frnM: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000225.5	1.00	PKS	frenolicin B	Streptomyces roseofulvus
BGC0000231.5	0.77	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001723.5	0.66	PKS	oviedomycin	Streptomyces antibioticus
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000234.5	0.63	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0000272.3	0.61	PKS	spore pigment	Streptomyces collinus
BGC0002276.2	0.59	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000275.4	0.58	PKS	tetracenomycin C	Streptomyces glaucescens
BGC0001197.5	0.57	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0002000.4	0.56	PKS	setomimycin	Streptomyces aurantiacus JA 4570

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000225.5	1.00	PKS	frenolicin B	Streptomyces roseofulvus
BGC0000231.5	0.77	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001723.5	0.66	PKS	oviedomycin	Streptomyces antibioticus
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000234.5	0.63	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0000272.3	0.61	PKS	spore pigment	Streptomyces collinus
BGC0002276.2	0.59	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000275.4	0.58	PKS	tetracenomycin C	Streptomyces glaucescens
BGC0001197.5	0.57	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0002000.4	0.56	PKS	setomimycin	Streptomyces aurantiacus JA 4570

Similar known clusters from MIBiG 4.0

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

frnK: mal

PKS_AT (18..312): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; Methoxymalonyl-CoA: 54.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 82.2
Methylmalonyl-CoA: 46.7
Methoxymalonyl-CoA: 33.9
Propionyl-CoA: 21.7
Ethylmalonyl-CoA: 17.1
inactive: 14.9
CHC-CoA: 11.2
Benzoyl-CoA: 8.8
2-Methylbutyryl-CoA: 4.6
trans-1,2-CPDA: 3.7
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 25306
Starter unit(s):: butyryl-CoA (Score: 1310.0; E-value: 0)
Malonyl elongations:: 7 (Score: 701.3; E-value: 7.4e-214)
Product class(es):: benzoisochromanequinone
Product molecular weight(s):: butyryl-CoA_7: 335 Da

frnI: KSIII (Score: 411.2; E-value: 7.4e-126); KSIII AAC18104.1_Frenolicin_butyryl-CoA (Score: 650.0; E-value: 0.0)
frnJ: ACP (Score: 78.5; E-value: 6.9e-25)
frnK: AT (Score: 391.7; E-value: 6.1e-120); AT AAC18106.1_Frenolicin_butyryl-CoA (Score: 660.0; E-value: 0.0)
frnL: KS (Score: 750.1; E-value: 1.4e-228)
frnM: CLF 7 (Score: 701.3; E-value: 7.4e-214)
frnN: ACP (Score: 103.4; E-value: 1.1e-32)
frnP: KR C9 (Score: 431.1; E-value: 3.2e-132)
frnQ: CYC C7-C12 (Score: 373.9; E-value: 1.2e-114)
frnR: CYC C5-C14 (Score: 471.6; E-value: 2.1e-144)