LOCUS       BGC0000234              7064 bp    DNA     linear   BCT 15-OCT-2012
DEFINITION  Streptomyces venezuelae JadJ (jadJ), jadomycin polyketide synthase
            cyclase (jadI), jadomycin polyketide ketosynthase (jadA), chain
            length determinant, acyl carrier protein (acp), ketoreductase, and
            bifunctional cyclase/dehydrase genes, complete cds.
ACCESSION   BGC0000234
VERSION     BGC0000234
KEYWORDS    .
SOURCE      Streptomyces venezuelae ATCC 10712
  ORGANISM  Streptomyces venezuelae ATCC 10712
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     On Jun 9, 1999 this sequence version replaced L33245.1.
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 13:13:45
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7064
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /db_xref="taxon:953739"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces venezuelae ATCC 10712"
                     /strain="ISP 5230"
                     /type_material="type strain of Streptomyces venezuelae"
     subregion       1..7064
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="PKS"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7064
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            153..1907
                     /gene="jadJ"
     CDS             153..1907
                     /codon_start=1
                     /gene="jadJ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1090:acetyl-CoA carboxylase biotin carboxylase (Score:
                     695.8; E-value: 1.1e-210)"
                     /gene_kind="biosynthetic-additional"
                     /product="JadJ"
                     /protein_id="AAD37851.1"
                     /sec_met_domain="ATP-grasp (E-value: 1.3e-11, bitscore:
                     33.3, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKVLIANRGEIAVRVARACRDAGIGSVAVYAEPDRDALHVRAAD
                     EAFALGGDTPATSYLDMAKVLQAAADSGADAVHPGYGFLSENAEFAQAVLDAGLTWIGP
                     PPQAIRDLGDKVAARHIAQRAGAPLVAGTPDPVSGADEVVAFAEEHGLPIAIKAAFGGG
                     GRGLKVARTLEEVPELYDSAVREAVAAFGRGECFVERYLDKPRHVETQCLADQHGNVVV
                     VSTRDCSLQRRHQKLVEEAPAPFLSEAQNAELYAASKAILKEAGYVGAGTVEFLVGTDG
                     TISFLEVNTRLQVEHPVTEEVTGIDLVREMFRIADGEELGYGDPEIRGHSFEFRINGED
                     PGRGFLPAPGTVTEFTPPTGPGVRLDAGVESGSVIGPAWDSLLANVIVTGASREQALQR
                     AARAVAEFRVEGMATAIPFHQAVVTDPDFTADPFRVHTRWIETEFVNEIKPFAPAGEAD
                     EDEAGRETIVVEVGGKRLEVSLPSSLGMTLARTVLRRVPKPKRRASKKVRASAASGDTL
                     ASPMQGTIVKVAVEEGQEVKEGDLIVVLEAMKMEQPLNAHRSGTVKGLTAEVGASVTSG
                     ATICDIKD"
     gene            2020..2349
                     /gene="jadI"
     CDS             2020..2349
                     /codon_start=1
                     /function="jadomycin biosynthesis"
                     /gene="jadI"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1225:Polyketide synthesis cyclase (Score: 161.3;
                     E-value: 1.1e-49)"
                     /gene_kind="biosynthetic-additional"
                     /note="JadI"
                     /product="jadomycin polyketide synthase cyclase"
                     /protein_id="AAD37852.1"
                     /transl_table=11
                     /translation="MHSTLIVARMEPGSSTDVAKLFAEFDASEMPHLMGTRRRQLFSYR
                     GLYFHLQDFDADNGGELIERAKTDPRFVGISEDLKPFIEAYDPATWRSPADAMATRFYN
                     WEANA"
     gene            2346..3614
                     /gene="jadA"
                     /gene_synonym="PKS"
     CDS             2346..3614
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (5-386). E-value:
                     8.1e-52. Score: 168.4. Matches aSDomain:
                     nrpspksdomains_jadA_PKS_KS.1"
                     /codon_start=1
                     /gene="jadA"
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2ks"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 472.7; E-value:
                     1.8e-143)"
                     /gene_kind="biosynthetic"
                     /gene_synonym="PKS"
                     /note="JadA"
                     /product="jadomycin polyketide ketosynthase"
                     /protein_id="AAB36562.1"
                     /sec_met_domain="t2ks (E-value: 1.9e-247, bitscore: 809.9,
                     seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTARRVVITGIEVLAPGGTGSKAFWNLLSEGRTATRGITFFDPTP
                     FRSRVAAEIDFDPEAHGLSPQEIRRMDRAAQFAVVAARAVADSGIDLAAHDPYRVGVTV
                     GSAVGATMGLDEEYRVVSDGGRLDLVDHAYAVPHLYDYMVPSSFSAEVAWAVGAEGPNT
                     VVSTGCTSGLDSVGYARGELIREGSADVMIAGSSDAPISPITMACFDAIKATTNRYDDP
                     AHASRPFDGTRNGFVLGEGAAVFVLEELESARARGAHIYAEIAGYATRSNAYHMTGLRP
                     DGAEMAEAIRVALDEARMNPTEIDYINAHGSGTKQNDRHETAAFKKSLGDHAYRTPVSS
                     IKSMVGHSLGAIGSIEIAASALAMEHNVVPPTGNLHTPDPECDLDYVRSCREQLTDSVL
                     TVGSGFGGFQSAMVLARPERKIA"
     aSDomain        2361..3503
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_jadA_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="8.10E-52"
                     /label="jadA_PKS_KS.1"
                     /locus_tag="jadA"
                     /protein_end="386"
                     /protein_start="5"
                     /score="168.4"
                     /tool="antismash"
                     /translation="VVITGIEVLAPGGTGSKAFWNLLSEGRTATRGITFFDPTPFRSRV
                     AAEIDFDPEAHGLSPQEIRRMDRAAQFAVVAARAVADSGIDLAAHDPYRVGVTVGSAVG
                     ATMGLDEEYRVVSDGGRLDLVDHAYAVPHLYDYMVPSSFSAEVAWAVGAEGPNTVVSTG
                     CTSGLDSVGYARGELIREGSADVMIAGSSDAPISPITMACFDAIKATTNRYDDPAHASR
                     PFDGTRNGFVLGEGAAVFVLEELESARARGAHIYAEIAGYATRSNAYHMTGLRPDGAEM
                     AEAIRVALDEARMNPTEIDYINAHGSGTKQNDRHETAAFKKSLGDHAYRTPVSSIKSMV
                     GHSLGAIGSIEIAASALAMEHNVVPPTGNLHTPDPECDLDY"
     CDS             3611..4825
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (68-363). E-value:
                     4e-29. Score: 93.6. Matches aSDomain:
                     nrpspksdomains_AAB36563.1_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2clf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093:Beta-ketoacyl synthase (Score: 685.4; E-value:
                     3.4e-208)"
                     /gene_kind="biosynthetic"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /product="chain length determinant"
                     /protein_id="AAB36563.1"
                     /sec_met_domain="t2clf (E-value: 1.5e-218, bitscore: 714.1,
                     seeds: 26, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSASVVVTGLGVAAPNGLGREDFWASTLGGKSGIGPLTRFDPTGY
                     PARLAGEVPGFAAEEHLPSRLLPQTDRMTRLALVAADWALADAGVRPEEQDDFDMGVVT
                     ASASGGFEFGQGELQKLWSQGSQYVSAYQSFAWFYAVNSGQISIRNGMKGPSGVVVSDQ
                     AGGLDAVAQARRQIRKGTRLIVSGGVDASLCPWGWVAHVASDRLSTSEEPARGYLPFDR
                     EAQGHVPGEGGAILVMEAAEAARERGARIYGEIAGYGSTFDPRPGSGREPGLRKAIELA
                     LADAGAAPGDIDVVFADAAAVPELDRVEAEALNAVFGTGAVPVTAPKTMTGRLYSGAAP
                     LDLAAAFLAMDEGVIPPTVNVEPDAAYGLDLVVGGPRTAEVNTALVIARGHGGFNSAMV
                     VRSAN"
     aSDomain        3815..4699
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAB36563.1_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="4.00E-29"
                     /label="AAB36563.1_PKS_KS.1"
                     /locus_tag="AAB36563.1"
                     /protein_end="363"
                     /protein_start="68"
                     /score="93.6"
                     /tool="antismash"
                     /translation="QTDRMTRLALVAADWALADAGVRPEEQDDFDMGVVTASASGGFEF
                     GQGELQKLWSQGSQYVSAYQSFAWFYAVNSGQISIRNGMKGPSGVVVSDQAGGLDAVAQ
                     ARRQIRKGTRLIVSGGVDASLCPWGWVAHVASDRLSTSEEPARGYLPFDREAQGHVPGE
                     GGAILVMEAAEAARERGARIYGEIAGYGSTFDPRPGSGREPGLRKAIELALADAGAAPG
                     DIDVVFADAAAVPELDRVEAEALNAVFGTGAVPVTAPKTMTGRLYSGAAPLDLAAAFLA
                     MDEGVIPPTVNVEP"
     gene            4868..5137
                     /gene="acp"
     CDS             4868..5137
                     /NRPS_PKS="Domain: ACP (12-82). E-value: 5.5e-17. Score:
                     53.7. Matches aSDomain: nrpspksdomains_acp_ACP.1"
                     /codon_start=1
                     /gene="acp"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1129:acyl carrier protein (Score: 121.3; E-value:
                     2.1e-37)"
                     /gene_kind="biosynthetic-additional"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /product="acyl carrier protein"
                     /protein_id="AAB36564.1"
                     /sec_met_domain="PP-binding (E-value: 2.1e-11, bitscore:
                     33.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSKTFTLDDLKRILREGAGADEGVDLDGDILDTHFETLGYESLA
                     LLETGSRIEREYSITLDDDVLSDADTPRALIEAVNAHLSAVAAA"
     aSDomain        4904..5113
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_acp_ACP.1"
                     /evalue="5.50E-17"
                     /label="acp_ACP.1"
                     /locus_tag="acp"
                     /protein_end="82"
                     /protein_start="12"
                     /score="53.7"
                     /tool="antismash"
                     /translation="RILREGAGADEGVDLDGDILDTHFETLGYESLALLETGSRIEREY
                     SITLDDDVLSDADTPRALIEAVNAH"
     CDS             5211..6005
                     /NRPS_PKS="Domain: PKS_KR (10-164). E-value: 5.2e-11.
                     Score: 34.6. Matches aSDomain:
                     nrpspksdomains_AAB36565.1_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     255.2; E-value: 1.1e-77)"
                     /gene_kind="biosynthetic-additional"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /product="ketoreductase"
                     /protein_id="AAB36565.1"
                     /sec_met_domain="adh_short (E-value: 1.9e-37, bitscore:
                     117.9, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQQEKQEKRVALVTGATSGIGLAVARLLASQNHRVFLGARNAEN
                     VAATVKQLQDEGLEVDGTTLDVRSGEDVKAFVQAAVDRFGTVELLVNNAGRSGAGVTAD
                     IADELWNDVIDTNLNCVFRLTREALTTGGMREKSRGRIINMASTAGKQGVVLGAPYSAS
                     KHGVVGFTKALGNELAPTGITVNAVCPGYVETPMAQRVRQGYAAAYETSEDAILEKFQS
                     KIPLGRYSTPEEVAGLVGYLASDTAASITSQALNVCGGLGNF"
     aSDomain        5241..5702
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAB36565.1_PKS_KR.1"
                     /evalue="5.20E-11"
                     /label="AAB36565.1_PKS_KR.1"
                     /locus_tag="AAB36565.1"
                     /protein_end="164"
                     /protein_start="10"
                     /score="34.6"
                     /tool="antismash"
                     /translation="VALVTGATSGIGLAVARLLASQNHRVFLGARNAENVAATVKQLQD
                     EGLEVDGTTLDVRSGEDVKAFVQAAVDRFGTVELLVNNAGRSGAGVTADIADELWNDVI
                     DTNLNCVFRLTREALTTGGMREKSRGRIINMASTAGKQGVVLGAPYSASK"
     CDS_motif       5247..5315
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AAB36565.1_0001"
                     /evalue="1.40E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="AAB36565.1"
                     /protein_end="35"
                     /protein_start="12"
                     /score="21.0"
                     /tool="antismash"
                     /translation="LVTGATSGIGLAVARLLASQNHR"
     CDS             6046..6978
                     /NRPS_PKS="Domain: Polyketide_cyc2 (4-145). E-value: 7e-14.
                     Score: 44.3. Matches aSDomain:
                     nrpspksdomains_AAB36566.1_Polyketide_cyc2.1"
                     /NRPS_PKS="Domain: Polyketide_cyc2 (154-268). E-value:
                     8.7e-09. Score: 27.8. Matches aSDomain:
                     nrpspksdomains_AAB36566.1_Polyketide_cyc2.2"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1128:cyclase/dehydrase (Score: 446.9; E-value:
                     6.2e-136)"
                     /gene_kind="biosynthetic-additional"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /product="bifunctional cyclase/dehydrase"
                     /protein_id="AAB36566.1"
                     /transl_table=11
                     /translation="MTTREVEHEITVAAPAQAVYRLIAEVENWPRIFPPTLYVDHVERG
                     EREERIRIWATANGAPKNWTSKRTLDPDNLRITFRQEVSTPPVAAMATWIIEPVSATES
                     GSALLHDYRAIDDDPEGLKWVDEAVDRNSRSELAALKTNVELAHASEEITFSFEDTVQI
                     DGSAKDAYDFVNEANLWPERLPHVATVRFEEETPGLQSLEMDSRAKDGSTHTTKSYRVT
                     FPHHRIAYKQITLPALMTLHTGYWTFEENEDGVAASSQHTVVLNTENIARILGADATVA
                     DAREYVQTALSTNSRATLGYAKAYAEGKR"
     aSDomain        6058..6480
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAB36566.1_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="7.00E-14"
                     /label="AAB36566.1_Polyketide_cyc2.1"
                     /locus_tag="AAB36566.1"
                     /protein_end="145"
                     /protein_start="4"
                     /score="44.3"
                     /tool="antismash"
                     /translation="EVEHEITVAAPAQAVYRLIAEVENWPRIFPPTLYVDHVERGEREE
                     RIRIWATANGAPKNWTSKRTLDPDNLRITFRQEVSTPPVAAMATWIIEPVSATESGSAL
                     LHDYRAIDDDPEGLKWVDEAVDRNSRSELAALKTNVE"
     aSModule        6058..6849
                     /domains="nrpspksdomains_AAB36566.1_Polyketide_cyc2.1"
                     /domains="nrpspksdomains_AAB36566.1_Polyketide_cyc2.2"
                     /incomplete
                     /locus_tags="AAB36566.1"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        6508..6849
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAB36566.1_Polyketide_cyc2.2"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="8.70E-09"
                     /label="AAB36566.1_Polyketide_cyc2.2"
                     /locus_tag="AAB36566.1"
                     /protein_end="268"
                     /protein_start="154"
                     /score="27.8"
                     /tool="antismash"
                     /translation="FSFEDTVQIDGSAKDAYDFVNEANLWPERLPHVATVRFEEETPGL
                     QSLEMDSRAKDGSTHTTKSYRVTFPHHRIAYKQITLPALMTLHTGYWTFEENEDGVAAS
                     SQHTVVLNTE"
ORIGIN
        1 gaattctgac ggatccctga atcggtgagt cttgaacgaa gtcctggacc ggaacaagct
       61 tgtcgtgaag gtccggtgca ccgtcttcga gccctttcgg gaaaagcgga acgggtacgg
      121 agaaggcgag tcgatcagga gttgaaaccg tcgtgcgcaa ggtgctcatc gccaaccgtg
      181 gcgaaatcgc tgtccgtgtg gcccgggcgt gccgggacgc gggtatcggc agcgtggccg
      241 tgtacgccga gccggaccgg gatgctctgc atgtccgggc ggctgacgag gcgttcgctc
      301 tgggcggtga cactccggcg accagttatc tggacatggc gaaggtgttg caggccgccg
      361 cggactcggg tgcggacgcg gtccaccccg gttacgggtt cctttcggag aacgcggagt
      421 tcgcgcaggc ggtcctcgac gcgggtctga cgtggatcgg tccgccgccg caggcgatcc
      481 gggatctggg tgacaaggtg gcggcccgtc acatcgccca gcgtgccggg gccccgctgg
      541 tggccggcac gcccgacccg gtctcgggtg cggacgaggt cgtggcgttc gccgaggagc
      601 acggtctgcc gatcgcgatc aaggcggcgt tcggtggcgg tggccgtggt ctgaaggtgg
      661 cgcggacgct ggaggaggtc ccggagctgt acgactccgc ggtccgtgag gcggtggcgg
      721 cgttcgggcg gggcgagtgc ttcgtggagc ggtacctgga caagccgcgg cacgtggaga
      781 cgcagtgcct ggccgaccag cacggcaacg tggtcgtcgt ctcgacgcgt gactgctcgc
      841 tgcagcgccg tcaccagaag ctggtggagg aggctccggc gccgttcctg tcggaggctc
      901 agaacgcgga gctgtacgcg gcgtcgaagg cgatcctgaa ggaggccggt tacgtcggtg
      961 ccggcacggt ggagttcctc gtcggcacgg acggcacgat ctcgttcctc gaggtcaaca
     1021 cccgtctgca ggtggagcac ccggtgacgg aggaggtcac ggggatcgac ctggtgcggg
     1081 agatgttccg gatcgcggac ggtgaggagc tcggctacgg cgacccggag atccggggtc
     1141 actccttcga gttccggatc aacggtgagg acccgggccg tggtttcctc ccggcgcccg
     1201 gcaccgtcac cgagttcacg ccgccgaccg gtccgggtgt ccgcctcgac gcgggtgtgg
     1261 agtcgggttc cgtgatcggc cccgcgtggg actcgctgct tgcgaacgtg atcgtgaccg
     1321 gtgcgagccg tgagcaggcg ctgcagcgtg cggcgcgtgc cgtggcggag ttccgcgtcg
     1381 agggcatggc gaccgcgatc cccttccacc aggcggtggt gaccgacccg gacttcaccg
     1441 cggacccgtt ccgggtgcac acgcggtgga tcgagacgga gttcgtcaac gagatcaagc
     1501 cgttcgcgcc ggcgggggag gcggacgagg acgaggccgg ccgcgagacg atcgtggtcg
     1561 aggtcggcgg caagcgtctt gaggtctccc tgccgtcctc gctcgggatg acgctggccc
     1621 ggacggtctt gcggcgggtg ccgaagccga agcggcgggc gtcgaagaag gtccgggctt
     1681 cggccgcctc gggtgacacc ctcgcctccc cgatgcaggg cacgatcgtg aaggtcgcgg
     1741 tcgaggaggg ccaggaggtc aaggaaggcg atctgatcgt cgtcctggag gccatgaaga
     1801 tggaacagcc cctcaacgcc caccgctccg gcaccgtgaa gggcctgacc gccgaagtcg
     1861 gcgcgtccgt cacctccggc gccaccatct gcgacatcaa ggactgaatt cacccgccgc
     1921 ccccgcgcgc cctttcgtac caccccgcta tttcccttcg ctttccctgc cttcttcagc
     1981 acgtcctttt ccaggacttc ttccggtgag gagaaacaca tgcacagcac tctgatcgtg
     2041 gcccgaatgg aacccggatc gagcaccgac gtggcgaagc tgttcgccga attcgacgcc
     2101 tccgagatgc cgcatctcat ggggacgcga cgccgtcagc tgttctcgta ccgcggcctc
     2161 tacttccacc tccaggactt cgacgccgac aacggcggcg agctcatcga gcgggccaag
     2221 accgaccccc gcttcgtggg catcagcgag gacctgaagc cgttcatcga ggcctacgac
     2281 ccggccacct ggcgctcgcc cgccgacgcc atggccaccc gcttctacaa ctgggaggcg
     2341 aacgcgtgac cgcgcgacgc gtcgtcatca ccggcatcga ggtcctcgcc cccggtggca
     2401 ccggatccaa ggccttctgg aacctgctca gcgagggccg cacggccacc cggggcatca
     2461 ccttcttcga cccgacgccg ttccgctccc gggtcgccgc cgagatcgac ttcgacccgg
     2521 aggcccacgg cctcagcccg caggagatcc gccgcatgga ccgggccgcc cagttcgcgg
     2581 tggtcgccgc gcgcgccgtc gccgacagcg gcatcgacct ggccgcccac gacccgtacc
     2641 gcgtcggcgt caccgtcggc agcgccgtcg gcgccaccat gggcctcgac gaggagtacc
     2701 gggtcgtcag cgacggcggc cggctcgacc tcgtcgacca cgcctacgcg gtgccgcacc
     2761 tctacgacta catggtgccc agctccttct ccgccgaggt cgcctgggcc gtcggcgccg
     2821 agggccccaa caccgtggtc tccaccggct gcacctccgg cctcgactcc gtcggctacg
     2881 cgcgtgggga gctgatccgc gagggctccg ccgacgtcat gatcgccggt tcctcggacg
     2941 ccccgatctc cccgatcacg atggcctgct tcgacgccat caaggcgacc accaaccggt
     3001 acgacgaccc ggcgcacgcc tcccggccct tcgacggcac ccgcaacggc ttcgtgctcg
     3061 gcgagggcgc ggccgtcttc gtcctggagg agctggagag cgcccgggcc cgcggcgccc
     3121 acatctacgc cgagatcgcc ggctacgcca cccgcagcaa cgcgtaccac atgaccggtc
     3181 tgcggccgga cggcgcggag atggcggagg cgatccgggt cgcgctcgac gaggcgcgga
     3241 tgaaccccac cgagatcgac tacatcaacg cgcacggctc cggcaccaag cagaacgacc
     3301 gccacgagac cgcagcgttc aagaagagcc tcggggacca cgcgtaccgc accccggtca
     3361 gctccatcaa gtcgatggtc gggcactccc tcggtgccat cggctccatc gagatcgccg
     3421 cctcggccct ggccatggag cacaacgtgg tcccgccgac gggcaacctg cacaccccgg
     3481 accccgagtg cgacctggac tacgtccgct cgtgccgcga gcagctcacg gactcggtcc
     3541 tgaccgtcgg cagcggattc ggcggcttcc agagcgccat ggtcctggcc cgtcccgaga
     3601 ggaagatcgc atgagcgcgt ccgtggtggt gaccggcctc ggtgtcgccg cacccaacgg
     3661 cctgggccgc gaggacttct gggcctcgac cctcggcggg aagagcggca tcggcccgct
     3721 cacccgcttc gaccccaccg gctacccggc gcgcctcgcc ggcgaggtgc ccggcttcgc
     3781 cgccgaggag cacctgccga gccggctgct gccgcagacc gaccggatga cccgcctcgc
     3841 gctggtcgcc gcggactggg cgctggccga cgcgggcgtc cgccccgagg agcaggacga
     3901 cttcgacatg ggcgtggtca cggccagcgc ctccggcggc ttcgagttcg gccagggcga
     3961 gctgcagaag ctgtggagcc agggcagcca gtacgtcagc gcgtaccagt ccttcgcctg
     4021 gttctacgcc gtcaacagcg gccagatctc catccgcaac ggcatgaagg gccccagcgg
     4081 cgtggtcgtc agcgaccagg ccggcggtct ggacgccgtc gcgcaggccc gccgacagat
     4141 ccgcaagggc acccggctca tcgtctccgg tggcgtcgac gcctcgctct gcccctgggg
     4201 ctgggtggcg cacgtcgcct ccgaccggct cagcaccagc gaggagcccg cccggggcta
     4261 cctgcccttc gaccgcgaag cccagggcca cgtgcccggc gagggtgggg cgatcctcgt
     4321 catggaggcc gccgaggcgg cccgggagcg cggggcccgg atctacggcg agatcgccgg
     4381 atacggctcc accttcgacc cccggccggg cagcgggcgc gagcccggcc tgcgcaaggc
     4441 catcgaactc gccctggccg acgccggagc ggcacccggt gacatcgacg tcgtcttcgc
     4501 ggacgcggcc gccgtccccg agctcgaccg cgtcgaggcc gaggccctca acgccgtctt
     4561 cggcaccggc gccgtcccgg tcaccgcgcc caagacgatg accggccggc tgtactccgg
     4621 cgccgcgccg ctcgacctgg ccgccgcgtt cctcgcgatg gacgagggcg tcatcccgcc
     4681 caccgtcaac gtcgagccgg acgccgcgta cggcctcgac ctggtcgtcg gcgggccccg
     4741 taccgccgag gtgaacaccg ccctggtgat cgcccgcggt cacggcggct tcaactccgc
     4801 gatggtcgtc cgttccgcga actgaccttt cccccgcagt acctcacagt cacccaggag
     4861 aagaaccatg agcagcaaga ccttcaccct cgacgacctc aagcggatcc tgcgcgaggg
     4921 cgccggcgcc gacgagggcg tcgacctcga cggcgacatc ctcgacaccc acttcgagac
     4981 gctcggctac gagtcgctcg ccctgctgga gaccggcagc cgcatcgagc gcgagtactc
     5041 catcaccctc gacgacgacg tgctgtccga cgccgacacc ccgcgcgccc tcatcgaggc
     5101 cgtcaacgcg cacctgtcgg ccgtcgccgc cgcctgaccc acccgaccga caccggcgcc
     5161 cgtacccgcc gccgtaccgc cgccccctgg cagagaagag acgacaccgc atgtcccagc
     5221 aggagaagca ggagaagagg gtcgccctcg tcaccggagc caccagtggc atcggcctcg
     5281 ccgtcgcccg gctcctggcg tcccagaacc accgggtgtt cctcggtgcc cgcaacgccg
     5341 agaacgtcgc cgccaccgtc aagcagctcc aggacgaggg ccttgaggtc gacggcacca
     5401 ccctcgacgt gcgctccggc gaggacgtca aggccttcgt ccaggccgcc gtcgaccgct
     5461 tcggcaccgt cgagctcctc gtgaacaacg cgggccgcag cggcgcgggc gtcaccgccg
     5521 acatcgccga cgagctgtgg aacgacgtca tcgacaccaa cctcaactgc gtcttccggc
     5581 tcacccgcga ggccctgacc accggcggca tgcgcgagaa gtcccgcggc cggatcatca
     5641 acatggcctc gaccgccggc aagcagggcg tcgtcctcgg cgccccctac tcggcctcga
     5701 agcacggcgt cgtcggcttc accaaggccc tcggcaacga gctcgccccg accggcatca
     5761 ccgtcaacgc ggtctgcccc ggttacgtcg agacgccgat ggcccagcgc gtccgccagg
     5821 gctacgccgc cgcgtacgag acctccgagg acgccatcct cgagaagttc cagtcgaaga
     5881 tcccgctcgg ccgctactcc acgcccgagg aggtcgccgg tctcgtcggc tacctggcct
     5941 ccgacaccgc ggcctccatc acctcgcagg cgctcaacgt ctgcggcggc ctgggcaact
     6001 tctgatcccc gcaccccctc tcgcaccgtc ccgaggagtt tcacgatgac gacccgtgag
     6061 gtcgagcacg agatcaccgt cgcggccccg gcccaggccg tgtaccggct gatcgccgag
     6121 gtggagaact ggccgcggat cttcccgccc accctctacg tcgaccacgt cgagcgcggc
     6181 gagcgggagg agcgcatccg gatctgggcg accgccaacg gcgccccgaa gaactggacg
     6241 tccaagcgga ccctggaccc ggacaacctc cggatcacct tccgccagga ggtctccacc
     6301 ccgccggtcg ccgccatggc gacctggatc atcgagccgg tctccgcgac cgagtccgga
     6361 tccgccttgc tgcacgacta ccgggcgatc gacgacgacc ccgagggcct gaagtgggtc
     6421 gacgaggccg tcgaccgcaa ctcgcgctcc gagctcgccg ccctcaagac caacgtcgaa
     6481 ctcgcccacg cctccgagga gatcaccttc tccttcgagg acaccgtgca gatcgacggc
     6541 tccgccaagg acgcctacga cttcgtcaac gaggcgaacc tctggccgga gcggctgccg
     6601 cacgtcgcca ccgtgcgctt cgaggaggag accccgggcc tgcagagcct ggagatggac
     6661 tcccgggcca aggacggctc cacccacacg acgaagtcgt accgggtgac cttcccgcac
     6721 caccggatcg cctacaagca gatcaccctg ccggcgctca tgaccctgca caccggctac
     6781 tggacgttcg aggagaacga ggacggcgtc gccgcctcct cgcagcacac ggtcgtcctc
     6841 aacaccgaga acatcgcccg gatcctcggc gccgacgcca ccgtcgccga cgcccgcgag
     6901 tacgtgcaga cggcgctcag caccaacagc cgggccaccc tcgggtacgc caaggcctac
     6961 gccgagggca agcgctgaca tgacggagcc ccgccgggca ggagcgcccg caccggagtc
     7021 cccggacgcc ccggacaccc cggtcctcct ggacgccgac gtcc
//
