BGC0000238 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000238 (Streptomyces rishiriensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T2PKS,butyrolactone	1	61,508	High	lactonamycin	PKS

BGC0000238 - Region 1 - T2PKS,butyrolactone

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Location: 1 - 61,508 nt. (total: 61,508 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)
butyrolactone: AfsA

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 61,509
Label		BGC0000238

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

lct10: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

lct12: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

lct16: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

lct40: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

lct41: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

lct43: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

lct44: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Transferases: PPTase acyltransferase aminotransferase glycosyltransferase kinase methyltransferase transferase

lct17: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

lct25: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

lct35: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

lct36: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

lct46: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

lct49: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

lct53: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

lct55: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

lct8: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: esterase phosphatase

ABX71087.1: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

lct34: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: cyclase dehydratase

lct27: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

lct29: Polyketide synthesis cyclase

smcogs: SMCOG1225: Polyketide synthesis cyclase

lct47: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

lct48: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

lct28: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

lct31: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

lct32: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000238.5	1.22	PKS	lactonamycin	Streptomyces rishiriensis
BGC0000848.5	0.96	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000849.4	0.78	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0000850.5	0.63	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0000237.5	0.58	PKS	lactonamycin Z	Streptomyces sanglieri
BGC0002276.2	0.57	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000231.5	0.44	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0002631.2	0.41	ribosomal	Myxarylin	Pyxidicoccus fallax
BGC0000234.5	0.39	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0000278.3	0.38	PKS	urdamycin	Streptomyces fradiae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000238.5	0.99	PKS	lactonamycin	Streptomyces rishiriensis
BGC0000237.5	0.83	PKS	lactonamycin Z	Streptomyces sanglieri
BGC0000848.5	0.77	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000849.4	0.71	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0000278.3	0.70	PKS	urdamycin	Streptomyces fradiae
BGC0000850.5	0.65	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0002276.2	0.65	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000231.5	0.63	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000272.3	0.62	PKS	spore pigment	Streptomyces collinus
BGC0001128.4	0.61	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

lct28: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (9..416): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFFLCLLSK
Nearest Stachelhaus code(s):: Gly DLFFLCLLSK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 61508
Starter unit(s):: acetyl-CoA (Score: 0.0; E-value: 0)
Malonyl elongations:: 8|9 (Score: 655.2; E-value: 7.4e-200)
Product class(es):: tetracenomycin
Product molecular weight(s):: acetyl-CoA_8: 561 Da
acetyl-CoA_9: 603 Da

lct12: KR (Score: 167.1; E-value: 1.3e-51)
lct16: OXY (Score: 267.9; E-value: 4.2e-82)
lct24: ACP (Score: 104.9; E-value: 3.8e-33)
lct25: MET C11O (Score: 545.7; E-value: 8.4e-167)
lct26: ACP (Score: 35.0; E-value: 2.6e-11)
lct27: CYC C9-C14 (Score: 233.2; E-value: 2.3e-72)
lct29: CYC C4-C21/C2-C23|C2-C19 (Score: 142.7; E-value: 8.2e-45)
lct30: CYC C5-C18 (Score: 191.7; E-value: 1e-59)
lct31: KS (Score: 718.1; E-value: 7e-219)
lct32: CLF 8|9 (Score: 655.2; E-value: 7.4e-200)
lct35: MET C13O|C17O (Score: 510.8; E-value: 3.1e-156)
lct36: GT (Score: 467.7; E-value: 9.2e-143)
lct40: KR (Score: 208.4; E-value: 3.4e-64)
lct41: OXY (Score: 283.0; E-value: 1.2e-86)