BGC0000241

BGC0000241: lomaiviticin A biosynthetic gene cluster from Salinispora tropica CNB-440

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Location: 2,460,444 - 2,522,674 nt. (total: 62,231 nt).
This entry is originally from NCBI GenBank CP000667.1.

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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General

Compounds

Genes

Biosynthesis

History

NRPS/PKS domains

KnownClusterBlast

General information about the BGC

MIBiG accession	BGC0000241
Short description	lomaiviticin A biosynthetic gene cluster from Salinispora tropica CNB-440
Status	Quality: questionable The quality level of this entry. Status: active The status of this entry. Completeness: complete Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)	PKS (Type II) saccharide (hybrid/tailoring)
Loci	CP000667.1 2460444 - 2522674 via Knock-out studies
Compounds	lomaiviticin A lomaiviticin C lomaiviticin D lomaiviticin E
Species	Salinispora tropica CNB-440 [taxonomy]
References	[1] Lomaiviticins A and B, potent antitumor antibiotics from Micromonospora lomaivitiensis. He H et al., J Am Chem Soc (2001) PMID:11457405 [2] Isolation of lomaiviticins C-E, transformation of lomaiviticin C to lomaiviticin A, complete structure elucidation of lomaiviticin A, and structure-activity analyses. Woo CM et al., J Am Chem Soc (2012) PMID:22963534 [3] Bioactivity-guided genome mining reveals the lomaiviticin biosynthetic gene cluster in Salinispora tropica. Kersten RD et al., Chembiochem (2013) PMID:23649992 [4] Lomaiviticin biosynthesis employs a new strategy for starter unit generation. Waldman AJ et al., Org Lett (2014) PMID:24383813 [5] The cytotoxicity of (-)-lomaiviticin A arises from induction of double-strand breaks in DNA. Colis LC et al., Nat Chem (2014) PMID:24848236 [6] Discovery of the lomaiviticin biosynthetic gene cluster in . Janso JE et al., Tetrahedron (2014) PMID:25045187

Chemical products information

lomaiviticin A Evidence:

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C₆₈H₈₀N₆O₂₄

Chemical database entries
NPAtlas
PubCHEM
ChemSpider

lomaiviticin C Evidence:

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C₆₈H₈₄N₄O₂₄

lomaiviticin D Evidence:

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C₆₉H₈₆N₄O₂₄

lomaiviticin E Evidence:

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C₇₀H₈₆N₄O₂₄

Chemical database entries
NPAtlas

List of genes involved in compound(s) production

Identifiers	Position	Product	Functions	Evidence	Extra
Strop_2172 ABP54623.1	2460444 - 2462018 (+)	drug resistance transporter, EmrB/QacA subfamily	Resistance/immunity		copy AA seq copy Nt seq
Strop_2173 ABP54624.1	2462246 - 2462731 (+)	hypothetical protein			copy AA seq copy Nt seq
Strop_2174 ABP54625.1	2462891 - 2463721 (-)	hypothetical protein			copy AA seq copy Nt seq
Strop_2175 ABP54626.1	2463718 - 2464668 (-)	ABC transporter related	Transport		copy AA seq copy Nt seq
Strop_2176 ABP54627.1	2464643 - 2465158 (-)	Glyoxalase/bleomycin resistance protein/dioxygenase	Resistance/immunity		copy AA seq copy Nt seq
Strop_2177 ABP54628.1	2465303 - 2466079 (-)	hypothetical protein			copy AA seq copy Nt seq
Strop_2178 ABP54629.1	2466328 - 2467164 (-)	transcriptional activator domain	Regulation		copy AA seq copy Nt seq
Strop_2179 ABP54630.1	2467536 - 2467877 (-)	hypothetical protein			copy AA seq copy Nt seq
Strop_2180 ABP54631.1	2467910 - 2468755 (+)	transcriptional regulator, AraC family	Regulation		copy AA seq copy Nt seq
Strop_2181 ABP54632.1	2469003 - 2470436 (+)	NDP-hexose 2,3-dehydratase	Precursor biosynthesis		copy AA seq copy Nt seq
Strop_2182 ABP54633.1	2470489 - 2471154 (-)	hypothetical protein			copy AA seq copy Nt seq
Strop_2183 ABP54634.1	2471199 - 2471645 (-)	Carboxymuconolactone decarboxylase			copy AA seq copy Nt seq
Strop_2184 ABP54635.1	2471575 - 2472402 (-)	peptidase C26			copy AA seq copy Nt seq
Strop_2185 ABP54636.1	2472868 - 2474640 (+)	monooxygenase, FAD-binding	Tailoring		copy AA seq copy Nt seq
Strop_2186 ABP54637.1	2474803 - 2476269 (-)	protoporphyrinogen oxidase			copy AA seq copy Nt seq
Strop_2187 ABP54638.1	2476390 - 2477496 (-)	ParB domain protein nuclease			copy AA seq copy Nt seq
Strop_2188 ABP54639.1	2477548 - 2479059 (-)	monooxygenase, FAD-binding	Tailoring		copy AA seq copy Nt seq
Strop_2189 ABP54640.1	2479056 - 2480528 (-)	monooxygenase, FAD-binding	Tailoring		copy AA seq copy Nt seq
Strop_2190 ABP54641.1	2480681 - 2481472 (+)	short-chain dehydrogenase/reductase SDR	Scaffold biosynthesis		copy AA seq copy Nt seq
Strop_2191 ABP54642.1	2481527 - 2482399 (+)	NmrA family protein	Tailoring		copy AA seq copy Nt seq
Strop_2192 ABP54643.1	2482473 - 2483090 (-)	hypothetical protein			copy AA seq copy Nt seq
Strop_2193 ABP54644.1	2483105 - 2483434 (-)	Polyketide synthesis cyclase	Scaffold biosynthesis		copy AA seq copy Nt seq
Strop_2194 ABP54645.1	2483463 - 2484248 (-)	short-chain dehydrogenase/reductase SDR	Scaffold biosynthesis		copy AA seq copy Nt seq
Strop_2195 ABP54646.1	2484439 - 2485476 (+)	hydroxyneurosporene-O-methyltransferase			copy AA seq copy Nt seq
Strop_2196 ABP54647.1	2485530 - 2487041 (+)	monooxygenase, FAD-binding	Tailoring		copy AA seq copy Nt seq
Strop_2197 ABP54648.1	2487067 - 2488026 (+)	cyclase/dehydrase	Scaffold biosynthesis		copy AA seq copy Nt seq
Strop_2198 ABP54649.1	2488023 - 2489462 (+)	monooxygenase, FAD-binding			copy AA seq copy Nt seq
Strop_2199 ABP54650.1	2489449 - 2490927 (+)	monooxygenase, FAD-binding			copy AA seq copy Nt seq
Strop_2200 ABP54651.1	2490997 - 2491740 (+)	hypothetical protein			copy AA seq copy Nt seq
Strop_2201 ABP54652.1	2491809 - 2493692 (-)	hypothetical protein			copy AA seq copy Nt seq
Strop_2202 ABP54653.1	2493667 - 2494023 (-)	4Fe-4S ferredoxin, iron-sulfur binding domain protein			copy AA seq copy Nt seq
Strop_2203 ABP54654.1	2494093 - 2494887 (-)	hypothetical protein			copy AA seq copy Nt seq
Strop_2204 ABP54655.1	2494939 - 2496441 (-)	L-glutamine synthetase			copy AA seq copy Nt seq
Strop_2205 ABP54656.1	2496438 - 2497892 (-)	Amidase			copy AA seq copy Nt seq
Strop_2206 ABP54657.1	2498099 - 2499385 (+)	Adenylosuccinate lyase			copy AA seq copy Nt seq
Strop_2207 ABP54658.1	2499369 - 2499770 (+)	GCN5-related N-acetyltransferase			copy AA seq copy Nt seq
Strop_2208 ABP54659.1	2499843 - 2501612 (-)	ABC transporter related	Transport		copy AA seq copy Nt seq
Strop_2209 ABP54660.1	2501752 - 2502072 (-)	Rieske (2Fe-2S) domain protein			copy AA seq copy Nt seq
Strop_2210 ABP54661.1	2502319 - 2502729 (+)	Glyoxalase/bleomycin resistance protein/dioxygenase	Resistance/immunity		copy AA seq copy Nt seq
Strop_2211 ABP54662.1	2502798 - 2503595 (+)	Glyoxalase/bleomycin resistance protein/dioxygenase	Resistance/immunity		copy AA seq copy Nt seq
Strop_2212 ABP54663.1	2503680 - 2505242 (-)	TAP domain protein			copy AA seq copy Nt seq
Strop_2213 ABP54664.1	2505434 - 2506576 (-)	protein of unknown function DUF1205			copy AA seq copy Nt seq
Strop_2214 ABP54665.1	2506573 - 2507688 (-)	O-methyltransferase, family 2			copy AA seq copy Nt seq
Strop_2215 ABP54666.1	2507870 - 2508811 (+)	hypothetical protein			copy AA seq copy Nt seq
Strop_2216 ABP54667.1	2508877 - 2509863 (+)	NAD-dependent epimerase/dehydratase	Precursor biosynthesis		copy AA seq copy Nt seq
Strop_2217 ABP54668.1	2509873 - 2510484 (+)	dTDP-4-dehydrorhamnose 3,5-epimerase	Precursor biosynthesis		copy AA seq copy Nt seq
Strop_2218 ABP54669.1	2510535 - 2511548 (+)	oxidoreductase domain protein			copy AA seq copy Nt seq
Strop_2219 ABP54670.1	2511568 - 2512302 (-)	hypothetical protein			copy AA seq copy Nt seq
Strop_2220 ABP54671.1	2512374 - 2513414 (-)	protein of unknown function DUF1205			copy AA seq copy Nt seq
Strop_2221 ABP54672.1	2513431 - 2514642 (-)	DegT/DnrJ/EryC1/StrS aminotransferase	Precursor biosynthesis		copy AA seq copy Nt seq
Strop_2222 ABP54673.1	2514817 - 2515824 (+)	dTDP-glucose 4,6-dehydratase	Precursor biosynthesis		copy AA seq copy Nt seq
Strop_2223 ABP54674.1	2515821 - 2517092 (+)	beta-ketoacyl synthase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
Strop_2224 ABP54675.1	2517089 - 2518375 (+)	beta-ketoacyl synthase	Scaffold biosynthesis		copy AA seq copy Nt seq
Strop_2225 ABP54676.1	2518375 - 2518629 (+)	phosphopantetheine-binding	Scaffold biosynthesis		copy AA seq copy Nt seq
Strop_2226 ABP54677.1	2518611 - 2519594 (+)	acyl transferase domain protein	Precursor biosynthesis		copy AA seq copy Nt seq
Strop_2227 ABP54678.1	2519716 - 2520690 (+)	hypothetical protein	Precursor biosynthesis		copy AA seq copy Nt seq
Strop_2229 ABP54679.1	2521135 - 2521617 (+)	transcriptional regulator, AraC family			copy AA seq copy Nt seq
Strop_2230 ABP54680.1	2521802 - 2522674 (+)	Glucose-1-phosphate thymidylyltransferase	Precursor biosynthesis		copy AA seq copy Nt seq

Biosynthesis information

Annotation changelog

Entry version: 4

Date

Changes

Submitters

Reviewers

2022-10-07

Update chemical activity to schema version 2.11

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 3

Date

Changes

Submitters

Reviewers

2022-09-15

Removed gene names of 'No gene ID'

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 2

Date

Changes

Submitters

Reviewers

2019-10-16

Migrated from v1.4

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

2019-10-16

Updated compound(s) information (NPAtlas curation)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 1

Date

Changes

Submitters

Reviewers

2015-06-12

Submitted

(ID: UJYWT4SZRXDY2IOPYAIF6MII)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Detailed domain annotation

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