LOCUS       BGC0000249             12637 bp    DNA     linear   BCT 28-DEC-2000
DEFINITION  Streptomyces nogalater nogalamycin biosynthetic gene cluster,
            complete sequence.
ACCESSION   BGC0000249
VERSION     BGC0000249
KEYWORDS    .
SOURCE      Streptomyces nogalater
  ORGANISM  Streptomyces nogalater
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:15:37
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12637
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(<1..382)
                     /codon_start=1
                     /note="Orf10; similar to Streptomyces coelicolor A3 (2)
                     putative ADA-like regulatory protein"
                     /product="putative ADA-like regulatory protein"
                     /protein_id="AAG42846.1"
                     /transl_table=11
                     /translation="MTFHPSAAACQQAGFRACKRCRPDTSPGSPEWNRRADLVARAMRL
                     IADGVVDREGVPGLAARLGYSTRQVERQLLAELGAGPLALARAQRAQTARMLIETTALP
                     MADIAFAAGFSSIRGFNDTVREI"
     source          1..12637
                     /db_xref="ATCC:27451"
                     /db_xref="taxon:38314"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces nogalater"
                     /strain="ATCC 27451"
                     /type_material="type strain of Streptomyces nogalater"
     subregion       1..12637
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000249"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12637
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             complement(616..1470)
                     /codon_start=1
                     /note="Orf9"
                     /product="unknown"
                     /protein_id="AAG42847.1"
                     /transl_table=11
                     /translation="MNPALLDDFSLEMTGKDVPRLEEARDGIPPGTRINITFLAGEDTA
                     TRLTAARAVKRLGFVPVPHISARRLPSRPALEEFLAGLAAHGTAENVFVVGGDPARPEG
                     PYADALAVLRTGLLRRHGVRHVGIAGYPEGHPGITDRALWSALTDKAAAIDADRFGGDV
                     ITQFGFDADPVLAWLEALRARGVRLPVRVGVPGPAGVRRLLGYATRFGVGTSASVARKY
                     GFSLTNLMGTTGPDRFLHALADRYDPGRHGVVKVHFYTFGGLRATAEWVDGFRRQHTPA
                     PAP"
     CDS             2849..3583
                     /codon_start=1
                     /note="Orf8"
                     /product="putative acetoacetate decarboxylase"
                     /protein_id="AAG42848.1"
                     /transl_table=11
                     /translation="MRAEDVVRAPSTPLDAPAFPAGPYRFTDREYLNITYRTDPEALRR
                     VVPEPLRVAEPLVRFEVMRMPDVTGLGDYTEAGQLAVVEYEGEPGEYGISIHVDNFPAI
                     ASGREIGAFPKKAGRPRLYVDQDTLVGTLDHGTLPVARATMGYKHRPLNTEQAREELTR
                     PTFMLKKLPHYDGSPRICELVRTQIADIVVKGAWSGPARLQLFAHALAPLADLPVLEVV
                     SAAHVLTDLTLGRARVVHDYLA"
     CDS             complement(3877..4422)
                     /codon_start=1
                     /note="Orf7; similar to Streptomyces coelicolor A3 (2)
                     hypothetical protein SC9B5.17"
                     /product="unknown"
                     /protein_id="AAG42849.1"
                     /transl_table=11
                     /translation="MTTITLVQGDITRQHADALVNAANSSLLGGGGVDGAIHRRGGPAI
                     LAECRALRASRYGEGLPTGRAVATTAGDLDARWVIHTVGPVWSSTEDRSDLLASCYRES
                     LRLAGELGARTVAFPALSTGVYRWPMGDAARIAVETVRTTPTAVEEVRFVLFDTHAYDT
                     FARELGDAGHAGHAGHPG"
     CDS             complement(4469..5887)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222:dehydrogenase (Score: 86.8; E-value: 2.7e-26)"
                     /gene_kind="biosynthetic-additional"
                     /note="Orf6"
                     /product="putative dehydrogenase"
                     /protein_id="AAG42850.1"
                     /sec_met_domain="DAO (E-value: 2.8e-11, bitscore: 32.9,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSMLDAVVVGAGPNGLTAAVELARRGFSVALFEAKSTVGGGART
                     EELTLPGFRHDPCSAAHPLGVNSPAFRSLPLDRYGLEWLHAPLPMAHPFPDGTAAVLSR
                     SVAETAASFGPRDAGPYRRLVEPLLARWDTLVQDFMSLPLTALPRDPVTLARFGLAGLP
                     PSTWLMRRFKDERFKTLFAGLVAHVMAPLGGLATSAVGLVFALSAHARGWPVARGGSQA
                     VSDALAAYLRDLGGAVHTDFEVKRLDDLPPARAYVFDTSPAALARMPASATTTANYRYG
                     PSVFKIDYALDGPVPWTAPEARSAGTVQIGASRAEIGTALRAAAREGRAPERPFMITVQ
                     PSVADPTRAPEGKHVFWAYGHVPNGWTGDLTEAIERQLERFAPGFRDRVLARATAGPAE
                     MAVRNANYVGGDIACGATSGLQLLLRPRLSLFPYHTPHPAVFLCSSATPPGPGVHGMSG
                     HNAAKAVWRRLRQG"
     CDS             complement(5976..6821)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1207:inositol monophosphatase (Score: 186.6; E-value:
                     1.1e-56)"
                     /gene_kind="biosynthetic-additional"
                     /note="Orf5"
                     /product="putative inositol monophosphatase"
                     /protein_id="AAG42851.1"
                     /transl_table=11
                     /translation="MIEETETIEEFLARRSADVEEAVRKAAEAEILPRFRRLAAHEVDQ
                     KSGPHDLVTDADRLAELRLTEDLGALLPGSVVVGEEAVHANPVVYEALQGDTPVWIVDP
                     VDGTRQFVHGDDGFCTLVALAHRGTVVASWTYAAARDRFATAVRGRGAFLDGERLFAGP
                     PEPGRDLVVATSHPDYTTDEQKRALRALWTDGLAPRPCGSAGLEYLAVARGELDAVAFS
                     WEAAWDHAAGLLLVEEAGGTHLTLTGEPFRVTGGNALPFTAARDAATARRVAGLLRTAS
                     "
     CDS             complement(7090..8955)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1274:gamma-glutamyltranspeptidase (Score: 636.6;
                     E-value: 5.5e-193)"
                     /gene_kind="biosynthetic-additional"
                     /note="Orf4"
                     /product="putative gamma-glutamyl transferase"
                     /protein_id="AAG42852.1"
                     /transl_table=11
                     /translation="MRFTTRPTLQGTFGMVSSTHWLASQSAMAVLEDGGNAFDAAVAGA
                     FVLHVVEPHLNGPAGEVPILLAPAGGAVRVLCGQGVAPAGATPAHYRRLGLDLVPGTGP
                     LAAAVPGAFDAWLLLLRDHGTKPLDAVLKYAIGYAEHGHAPVENVGATVASVRRLFETE
                     WPSSAEVYLPGGRPPRPGTLLRNPALAATWKRLLAETAGAGDREARIDAAREVWRSGFI
                     AEALVRQSRRPTMDTSGERHTGTLTEADLAGWSAHYETPVTYDWNGWTVCKAGPWSQGP
                     VLLQQLALLPAELPAPGSADYVHLLVEGCKLAMADREAWYGDAAEVPLADLLSDAYNAG
                     RRALIGAKASYDLLPGSPGGRPPRLPALAHARVPADTRPGFDPMGAGEPTVAPLPGAPT
                     LAEATGQAQVTGEADVAADGSTRGDTCHLDVVDRWGNMIAATPSGGWLQSNPVVPELGF
                     PLGTRLQMTWLEEGLPGTLTPGRRPRTTLTPSLALRDGVPVLAFGTPGGDQQDQWQLHF
                     FLAAALGAPVRGGLDLQGAIDAPNWHNDSFPGSFHPRRRRPGSVTVESRMDPGVIEELR
                     RRGHDVTVGPAWSEGRLCAVARDPETGILSAAANPRGMQGYAVGR"
     gene            9291..10127
                     /gene="snogM"
     CDS             9291..10127
                     /NRPS_PKS="Domain: oMT (25-221). E-value: 6e-53. Score:
                     171.4. Matches aSDomain: nrpspksdomains_snogM_oMT.1"
                     /codon_start=1
                     /gene="snogM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 203.5; E-value:
                     6.5e-62)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative O-methyltransferase"
                     /product="SnogM"
                     /protein_id="AAG42853.1"
                     /sec_met_domain="PCMT (E-value: 1.9e-12, bitscore: 36.8,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNSGTRPQEVADFYDGSSRLIAELNGGSLHFGCWDSLPADAGMD
                     RASQRLTEMMTERIEVGPGQRVLDIGCGTGAPAVQLARATGAEVVGITISPEQVRLATA
                     HAEREGVAERVTFRCADASAELPFPADSFDAVWFFESIFHLPDRLTALRRAAEVLRPGG
                     RLALTDVLHNDETSPAAQESLDEHAYTPLVGEPMRLSDYPPLLRQARLVPVECRDISEL
                     TVGRTLECMHRTLDENRERFVERYGSELVDQFSTAVPLLDAVEFGYGIVTAARPDH"
     aSDomain        9366..9953
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_snogM_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="6.00E-53"
                     /label="snogM_oMT.1"
                     /locus_tag="snogM"
                     /protein_end="221"
                     /protein_start="25"
                     /score="171.4"
                     /tool="antismash"
                     /translation="NGGSLHFGCWDSLPADAGMDRASQRLTEMMTERIEVGPGQRVLDI
                     GCGTGAPAVQLARATGAEVVGITISPEQVRLATAHAEREGVAERVTFRCADASAELPFP
                     ADSFDAVWFFESIFHLPDRLTALRRAAEVLRPGGRLALTDVLHNDETSPAAQESLDEHA
                     YTPLVGEPMRLSDYPPLLRQARLVPVECRDISE"
     gene            10221..11042
                     /gene="snogL"
     CDS             10221..11042
                     /codon_start=1
                     /gene="snogL"
                     /note="putative O-methyltransferase"
                     /product="SnogL"
                     /protein_id="AAG42854.1"
                     /transl_table=11
                     /translation="MGNTPIELYLDLMSRTVSNTIYEDPQIDPRRYWELLRERDGDGTG
                     PVMDGPGNWSAYDSEGREQGSVWPRDAHTMVGMRRLRNVRECAERVLADGVPGDFMETG
                     VWRGGTCIFMRAVLAAYEVSDRVVWVADSFAGIPAPDLDRYPQDEEARGIESVNEVVGV
                     PLETVRGNFDRYGLLDDQVRFLPGRFCDTLPEAPVERLALLRLDGDLYESTMDALVSMY
                     PKLSPGGYLIVDDYHALDVCKKAVHDYRDQHGIDDPITDIDWSGAYWRKSA"
     CDS             complement(12321..>12637)
                     /codon_start=3
                     /gene="snoN"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /product="SnoN"
                     /protein_id="AAG42855.1"
                     /sec_met_domain="PhyH (E-value: 4.1e-11, bitscore: 32.9,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWMPRVTVPLRAGDCTFHHARTVHSAGANSTDEPRLSTSAVYMDA
                     TAAYRPTGIAFLDDLPGTGADPLREGAPLTGDRFPLLRRPQTRQPAPARYPLPQQGHGS
                     "
     gene            complement(12321..>12637)
                     /gene="snoN"
ORIGIN
        1 agatctcgcg gacggtgtcg ttgaagccgc gtatggagga gaagccggcc gcgaacgcga
       61 tgtcggccat gggcagcgcg gtggtctcga tgagcatccg cgcggtctgg gcgcgctggg
      121 cgcgggcgag cgcgaggggt ccggcgccca gttcggcgag gagttgccgt tccacctggc
      181 gggtgctgta gccgagccgg gcggcgagtc cgggcacgcc ctcgcggtcc acgacgccgt
      241 cggcgatcag ccgcatggcg cgggcgacca ggtcggcgcg ccggttccac tccggggagc
      301 cggggctggt gtcggggcgg cagcgcttgc aggcccggaa cccggcctgc tgacaggcgg
      361 cggcactcgg gtggaacgtc atgttctccg gcttgggcgg tacggccggg cagctcgggc
      421 ggcagtagat ccgggtggtc aggacggccg tgaagaacca cccgtcgaac cgcgcgtctt
      481 ggaccgcacc gcgcgcacgc agtgctcccg atcgaggaga gtgcctgtct gcatgcgtcc
      541 agcatcggtc acggcccgcc ccgtccgctg gcggaaatcc gacatcgacc cggcctggcc
      601 cggatcttca ccgtctcagg gtgcgggcgc gggagtgtgc tgccgccgga atccgtcgac
      661 ccactcggcg gtggcgcgca gtccgccgaa ggtgtagaag tgcaccttca ccacgccgtg
      721 ccggccgggg tcgtagcggt cggccagcgc gtgcaggaag cggtccgggc cggtggtgcc
      781 catgaggttg gtcagcgaga aaccgtactt gcgggcgacg gaggcgctgg tgccgacgcc
      841 gaaccgggtg gcgtagccca gcagccggcg caccccggcc ggtccgggga cgccgacccg
      901 gacgggcagg cgcacgcccc gggcgcgcag ggcctccagc caggcgagga ccgggtccgc
      961 gtcgaagccg aactgggtga tcacgtcccc gccgaaccgg tccgcgtcga tggcggccgc
     1021 cttgtccgtg agcgccgacc acagggcccg gtcggtgatg ccggggtggc cttccggata
     1081 gccggcgatg ccgacgtgcc ggacgccgtg gcggcgcagc agcccggtgc gcagcacggc
     1141 cagggcgtcc gcgtaggggc cctcgggccg ggcggggtcg ccgccgacga cgaacacgtt
     1201 ctcggcggtg ccgtgggccg ccagcccggc gaggaactcc tccagggcgg gccgggaggg
     1261 cagccggcgg gcggagatgt gcggtacggg gacgaagccg agccgcttga ccgcgcgggc
     1321 cgccgtgagc cgggtggcgg tgtcctcgcc ggccaggaag gtgatgttga tccgggtgcc
     1381 cggcgggatg ccgtcgcgcg cctcctccag ccggggcacg tccttgccgg tcatctccag
     1441 ggagaagtcg tccagcagag cggggttcat ggggcgtctc ggctttcgta cggctgttcc
     1501 cggacccggc cgatgatgtc acgcggacgg ccggccccga tgggcagggc gcccaccacc
     1561 gggccgacgg cgggggcgga gcgggatccg gtgccctcac cgacgacgcg gcccggcacg
     1621 gcacggcacg gctcggcccg gcacggcacg gctcagcacg gctcggcagg gccccgcgct
     1681 caccgcacct accgggccgt ccgcagcgct gcccggcggg ttgccggtgg aggcaggcgg
     1741 ggggttgccg acgggggcgg ggggcccggc agagggcagg cgggcccccg gcagagggca
     1801 ggcgggcccc cggcagggag cggcaggcag gtccccggca cagggcaggc gagtccccgg
     1861 cagggagcgg caggcaggtc cccggcaggg agcggcaggc aggtccccgg cagggaggga
     1921 caggcaggtc cccggcaggg agcggcaggc aggtccccgg cagggaggga caggcaggtc
     1981 cccggcacag ggcaggcgag tccccggcag ggagcggcag gcgggtcccc ggcaggggac
     2041 aggcaggcgc caggccggtt cccggcgggt ccccggccgg acgaccgccg cgtcaggtgt
     2101 cctcggtgac gcggggcagg tgagccctca gtgacgcccg tcaggcatcc tgggcgcccc
     2161 cgccaggagc ttcggcgccc ccgccgggca gcctcggcaa ccccgccaag tgtcctaggc
     2221 gacgcccgtc acgtgtcccc ggcaacggcg gaggcggcgg cgttgtagcg ggccagcagg
     2281 gtgcgcaggg ccctgacctc ctccggggac cagccggcgg tgcgggcgtc gacctcgcgg
     2341 tacatccggg cgtcggcgcg ggcgagcagt tcccggccgc ggtcgctcgg gcgcagcagc
     2401 tggacgcggc ggtttccggg gacggtctcg cgcaccagca ggccggcccg ttccagcgcg
     2461 gtgacctggc ggctgatcgt ggacttgttc agcccgtaca gcccgaccag ctcccccgcc
     2521 cgggccccgt cgcgctcggc cagctccgac agcagtgaat agtcgacgaa actcaggtcg
     2581 gggtggagcc gttcggcgtt ggcccgaaag cgccgggaga gggtcaccag ctcccggacg
     2641 agccccggga cctccgtgtc gtcgtcggtc acgtccgtcc gtccttcccc gcggtccggg
     2701 taggaatgat cacacagacc cgttgagttg ctgtttgcaa cactgtggcg accggcgtca
     2761 cggtggctta agttgcggat gtttgccgta cttcgacccc ttatgctccg gatagtccgg
     2821 aaggggccag gagaggcagg cgagagtcat gcgagccgag gacgtcgtcc gtgcaccgtc
     2881 gacaccgctg gatgccccgg ccttcccggc gggcccgtac cggttcaccg accgcgagta
     2941 cctgaacatc acctaccgga cggaccccga ggccctgcgc cgggtggtgc cggagccgct
     3001 gcgggtggcg gagccgctgg tccgcttcga ggtcatgcgg atgccggacg tcaccggtct
     3061 gggcgactac accgaggccg gtcagctggc cgtcgtggag tacgagggcg agccgggcga
     3121 gtacggcatc tcgatccacg tggacaattt ccccgccatc gcgagcggcc gggagatcgg
     3181 cgccttcccg aagaaggccg gccgcccccg gctctacgtc gaccaggaca ccctggtcgg
     3241 caccctcgac cacggcacgc tgcccgtggc ccgcgccacg atgggctaca agcaccggcc
     3301 gctgaacacg gagcaggccc gcgaggagct gacccggccg acgttcatgc tcaagaagct
     3361 gccgcactac gacggttcgc cgcgcatctg cgaactcgtc cggacgcaga tcgccgacat
     3421 cgtggtcaag ggcgcctgga gcggtcccgc acggctccag ctcttcgccc acgcgctggc
     3481 cccgctcgcg gacctgccgg tcctggaggt cgtctccgcc gcccacgtcc tgaccgatct
     3541 gaccctgggc cgcgcccggg tcgtccacga ctacctcgcc tgaccgctcc gccgcaccga
     3601 gagggaaacc gagcggagaa ttcctcagcc tcggcgtctt cgaccgggac cacgcccggc
     3661 agaccgctcc ggaccccgac ccggactccc cgcacgcctg cctcgacccg gacaccttca
     3721 ccgccggcac cccgtgcggc cgctaccagg gcgtcaccga ccaggtgatc atgtccagga
     3781 cgccgggacg cttccgcacc ctcctggtgc cgcgcggcgg cagcgccccg gtccggctgc
     3841 cccgccacgg ctgacccgca cccgggccgg ggacggtcaa ccggggtgac cggcgtgacc
     3901 ggcgtgaccg gcgtcgccga gttcccgggc gaacgtgtcg tacgcgtggg tgtcgaagag
     3961 gacgaaacgg acctcctcga cggcggtcgg ggtggtgcgt accgtctcca cggcaatgcg
     4021 ggccgcgtcc cccatcggcc agcggtagac gccggtggag agggcgggga acgccaccgt
     4081 acgggcgccg agttcgcctg ccaggcgcag ggactcgcgg tagcaggagg ccagcaggtc
     4141 cgagcggtcc tcggtggacg accagacggg acccaccgtg tggatcaccc agcgggcgtc
     4201 caggtcaccc gccgtggtgg cgaccgcccg gccggtgggc aggccctcgc cgtaccggga
     4261 ggcgcgcagc gcgcggcact ccgccaggat ggccgggccg ccgcgacggt ggatcgcgcc
     4321 gtccactccc ccgccgccca ggagcgagga gttggccgcg ttgacgaggg cgtcggcgtg
     4381 ctgccgggtg atgtcgccct ggacgagggt gatggtggtc atgtccgtct catcctttcg
     4441 gggcgactcg gtctcggctc gcgtcggttc agccctgtct cagtctccgc cagaccgcct
     4501 tggccgcatt gtggcccgac atgccgtgca cgccgggtcc cggcggcgtg gccgaggagc
     4561 agaggaagac ggccgggtgg ggggtgtggt acgggaacag ggacagtctg ggccgcagca
     4621 gcagctggag tccggaggtc gcgccgcagg cgatgtcccc gccgacgtag ttggcgttgc
     4681 ggacggccat ctcggcgggc ccggccgtcg cgcgggcgag cacccggtcg cggaagccgg
     4741 gggcgaagcg ctccagctgg cgttctatgg cctcggtgag gtccccggtc cagccgttcg
     4801 gcacatggcc gtacgcccag aagacgtgct tgccctcggg ggcccgggtg gggtcggcca
     4861 cgctcggctg cacggtgatc atgaacggcc gctcgggtgc gcgtccctcg cgggcggcgg
     4921 cgcgcagggc cgtgccgatc tcggcccggc tcgcgccgat ctggacggta ccggcgctcc
     4981 gggcctcggg cgcggtccac ggcaccgggc cgtccagcgc atagtcgatc ttgaagacgc
     5041 tggggccgta gcggtagttc gccgtagtag tcgccgaggc cggcatgcgg gcgagcgcgg
     5101 cgggcgaggt gtcgaagacg tacgcgcgcg cgggcggcag gtcgtccagg cgcttgacct
     5161 cgaagtcggt gtgcaccgca ccgccgagat ccctcagata cgcggcgagg gcgtcggaga
     5221 cggcctggga accgccgcgg gccaccggcc agccgcgggc gtgcgcggac agggcgaaga
     5281 ccaggccgac ggcgctggtg gcgaggccgc ccagcggtgc catcacatgg gcgacgaggc
     5341 cggcgaagag ggtcttgaac cgctcgtcct tgaagcggcg catcagccag gtggacggcg
     5401 ggagcccggc caggccgaag cgggcgaggg tgaccgggtc gcggggcagc gcggtcagcg
     5461 gcagggacat gaagtcctgg accagggtgt cccagcgggc gagcaggggc tcgaccagcc
     5521 tgcggtacgg gcccgcgtcg cgcggtccga aggaggcggc ggtctcggcc accgaacggg
     5581 acagcacggc cgcggtgccg tccgggaacg ggtgggccat gggcagcggg gcgtgcagcc
     5641 actcaaggcc gtaccggtcg agggggaggg agcggaacgc cggggagttg accccgaggg
     5701 ggtgcgcggc cgagcacggg tcgtgccgga agccgggcag cgtcagctcc tccgtgcggg
     5761 cgccgccgcc caccgtggac ttcgcctcga acagggccac ggagaagccg cggcgggcca
     5821 gctccaccgc ggcggtcagt ccgttcggcc ccgcacccac cacgaccgca tcgagcatcg
     5881 acggcacctt cggacccctt cgtcagccga tggccattcg ggatcaggat atgccggggc
     5941 gccggcactc ccccgatccg ggtggcccgg gccggtcagg aggcggtgcg cagcagcccg
     6001 gccacccggc gggcggtggc ggcgtcccgc gcggcggtga acggcagggc gttgccgccg
     6061 gtgacgcgga acggctcgcc ggtcagggtg aggtgcgtgc cgccggcctc ctcgaccagg
     6121 agcagtccgg ccgcgtggtc ccaggcggcc tcccaggaga acgcgacggc gtccagctcg
     6181 ccccgggcga ccgcgaggta ctccaggccc gcggagccgc acgggcgcgg ggcgaggccg
     6241 tcggtccaca gggcgcgcag cgcgcgcttc tgctcgtcgg tggtgtagtc cgggtgggag
     6301 gtggcgacga ccaggtcccg gccgggctcg ggcgggccgg cgaacagccg ctcgccgtcg
     6361 aggaaggcgc cccggccgcg gacggcggtg gcgaaccggt cgcgcgcggc ggcgtaggtc
     6421 caggaggcga ccacggtgcc gcggtgggcg agggccacca gggtgcagaa gccgtcgtcg
     6481 ccgtggacga actggcgggt gccgtcgacc gggtcgacga tccagaccgg ggtgtcgccc
     6541 tggagcgcct cgtagaccac cgggttggcg tgcaccgctt cctcgccgac caccacggag
     6601 ccgggcagca gcgcgccgag gtcctcggtg agccgtagct cggcgagccg gtcggcgtcc
     6661 gtgaccaggt cgtgcgggcc gctcttctgg tccacctcgt gcgcggcgag gcggcggaag
     6721 cggggcagga tctcggcctc ggcggccttg cggaccgcct cctccacgtc ggccgagcgg
     6781 cgggcgagaa actcctcgat ggtttccgtc tcttcgatca tgcctcccat cagaccacgt
     6841 cccactgaca cgcccccgcc cacgggtgcc cgtggggtgg aatcgacgtg aacagcgggg
     6901 gtctcccgaa ggggcgcccc ggtatggcgc cgggcggacc gtacggggcc gggaggacgg
     6961 gccgcacggg gccgggacgg gccgcacggg gccggacgcg gtggacgggg gccggcgcgg
     7021 tggacggggg ccggcgcggt ggacggggcc ggcgcggtgg acggccgggg cccgtcggcg
     7081 gccggggtgt cagcggccga ccgcgtaacc ctgcatcccg cgcgggttcg ccgccgccga
     7141 gaggatgccg gtctccgggt cccgggccac cgcgcacagc cggccctcgg accaggcggg
     7201 gccgacggtg acgtcgtggc cgcgccgccg cagctcctcg atcacaccgg ggtccatgcg
     7261 ggactccacg gtgacgctgc cggggcgccg gcggcgcggg tggaaggagc cggggaagct
     7321 gtcgttgtgc cagttggggg cgtcgatggc gccctggagg tcgaggccgc cgcggacggg
     7381 ggcacccagg gcggcggcca ggaagaagtg cagctgccac tggtcctgct ggtccccgcc
     7441 gggcgtgccg aacgccagga ccggtacccc gtcgcgcagg gcgagggagg gggtgagggt
     7501 ggtgcggggg cggcggccgg gggtgagggt gcccggcagg ccctcctcca gccaggtcat
     7561 ctgcagccgg gtgccgagcg ggaagccgag ttcggggacc acggggttgg actggagcca
     7621 gccgccgctg ggcgtggccg cgatcatgtt gccccagcgg tcgacgacgt ccaggtggca
     7681 ggtgtcgccc cgggtcgagc cgtcggcggc cacgtcggcc tcaccggtca cctgcgcctg
     7741 accggtggcc tcggcgaggg tgggcgcccc gggcagcggg gcgacggtcg gctccccggc
     7801 gcccatcggg tcgaagccgg gccgcgtgtc ggccggcacg cgcgcgtgcg ccagcgcggg
     7861 cagcctgggg ggccttccgc cggggctgcc ggggagcagg tcgtaggagg ccttcgcgcc
     7921 gatcagggcc cgccggccgg cgttgtaggc gtccgacagc aggtcggcga ggggcacctc
     7981 ggcggcgtcg ccgtaccagg cctcgcggtc ggccatggcg agcttgcagc cctcgaccag
     8041 caggtggacg tagtcggcgg agcccggtgc gggcagctcg gccgggagca gggcgagctg
     8101 ctggaggagc accgggccct ggctccaggg gccggccttg cacacggtcc agccgttcca
     8161 gtcgtacgtc accggtgtct cgtagtgggc ggaccagccg gccaggtcgg cctcggtgag
     8221 ggtgccggtg tgccgttcgc cgctggtgtc catggtgggc cgccgggact gccgtaccag
     8281 ggcctcggcg atgaagccgg agcgccacac ctcgcgcgcg gcgtcgatcc gcgcctcccg
     8341 gtcgccggcg cccgcggtct cggccagcag ccgcttccag gtggcggcga gcgcggggtt
     8401 gcgcagcagc gtcccgggcc ggggcggccg gccgccgggc aggtacacct cggccgacga
     8461 cggccactcg gtctcgaaca gccgccgtac gctcgccacg gtcgcgccga cgttctccac
     8521 gggcgcgtgc ccgtgctcgg cgtagccgat ggcgtacttg aggaccgcgt ccaggggctt
     8581 ggtgccgtgg tcgcgcagca aaagcagcca ggcgtcgaag gcgccgggca cggcggcggc
     8641 gagcggtccg gtgccgggca cgaggtccag cccgaggcgc cggtagtggg ccggggtggc
     8701 gccggccggg gcgacgccct gtccgcacag cacgcgcacg gcgccgcccg cgggggcgag
     8761 caggatcggc acctcgccgg ccggcccgtt cagatgcggc tccacgacgt gcagcacgaa
     8821 cgcgccggcg acggccgcgt cgaaggcgtt gccgccgtcc tccagcaccg ccatcgccga
     8881 ctgggaggcc agccagtgtg tggaggagac catgccgaag gtgccttgaa gggtgggtcg
     8941 ggtcgtgaac cgcatctctc acctcgttgt ttccacacgt cgcccgcgtc ggccggcgct
     9001 cgggaccact cctggcaaga ctccggagag ttcgggccgg cgtcgtcctc cgtccgccgc
     9061 tcccccggca gcctatcgat cgccatgcgt cccctcatcc ccgtctccgg cagaaatggg
     9121 cgtcgtacgt gcttcgcccg cccaccgtcc ggcgagcggt tctcgatccg ttccaccggg
     9181 tctcaagggg ggttccaagg tcccgtccta gcgtcccggg ggcagcaacg accgcgtgag
     9241 ccggcctcac ttgtgctcag cggccgtacg gaacagggag aggggaaccg atgacgaact
     9301 ccgggacacg gccgcaggag gtcgcggact tctacgacgg cagcagccgt ctgatcgccg
     9361 aactgaacgg agggagcctg cacttcggct gctgggactc gctgccggcc gacgccggca
     9421 tggaccgggc gtcgcagcgg ctcaccgaga tgatgacgga gcggatcgag gtcggccccg
     9481 gacagcgggt gctggacatc ggctgcggca ccggcgcgcc ggcggtgcaa ctggcccggg
     9541 ccaccggcgc cgaggtcgtg ggcatcacga tcagcccgga gcaggtccgg ctggccacgg
     9601 cacacgccga gcgggaggga gtcgccgagc gggtcacctt ccggtgcgcg gacgcctccg
     9661 cggaactccc cttccccgcc gactcgttcg acgcggtctg gttcttcgag tcgatcttcc
     9721 accttccgga ccggctgacc gcgctgcgcc gggcggccga ggtcctgcgg ccgggcgggc
     9781 gactggccct caccgacgtc ctgcacaacg acgaaacctc ccccgcggcc caggagtcgc
     9841 tggacgagca cgcgtacacg ccgttggtcg gcgagccgat gcggctgtcc gactatccgc
     9901 ctctgctgcg gcaggcccgc ctcgtccccg tcgagtgccg cgacatcagc gaactgaccg
     9961 tggggcgcac gctggagtgc atgcaccgca cgctcgacga gaaccgcgag cgcttcgtcg
    10021 agcggtacgg cagcgagctg gtcgatcagt tctccacggc cgtcccgctc ctggacgcgg
    10081 tcgagttcgg ctacggcatc gtcaccgcgg cccggccgga tcactgagca caccgtccgc
    10141 cgggcgtcgc gcgccggtcc gacggtcagc cttcctccca ccttctactg ccccttcact
    10201 gcgacagcga ggacggaccc atggggaaca cgccgatcga gctctacctg gatctgatgt
    10261 cacgcaccgt cagcaacacc atctacgagg atccgcagat cgacccgcgc cgctactggg
    10321 agctgctgcg ggaacgggac ggcgacggga ccggaccggt catggacggc cccggcaatt
    10381 ggagtgccta cgacagcgag ggacgcgagc agggcagcgt ctggccgcgc gacgcccaca
    10441 ccatggtcgg catgcgcagg ctcaggaacg tacgggaatg cgcggagcgg gtcctggccg
    10501 acggggtgcc gggcgacttc atggagaccg gcgtgtggcg cggcggcacc tgcatcttca
    10561 tgcgggcggt gctggcggcg tacgaggtca gcgaccgcgt ggtgtgggtc gccgactcct
    10621 tcgccggtat ccccgcgccc gacctggacc gctatccgca ggacgaggag gcgcggggca
    10681 tcgagtcggt caacgaggtg gtgggcgtgc ccctggagac cgtccgcggc aacttcgacc
    10741 gctacgggct gctcgacgac caggtgcggt tcctcccggg acggttctgc gacacgctgc
    10801 ccgaggcccc cgtggagcgg ctggcgctgc tgcgtctgga cggggatctg tacgaatcca
    10861 cgatggacgc cctggtgtcg atgtacccga agctgtcgcc cggcggctat ctgatcgtgg
    10921 acgactacca cgccctggac gtctgcaaga aggccgtgca cgactatcgg gaccagcacg
    10981 gcatcgacga cccgatcacg gacatcgact ggtcgggggc ctactggcgc aagagcgcct
    11041 gaacgcgcca ccaggcacgg gccgtacgga tggggcgatg gccctccgta cggcccgttc
    11101 gcgccggggt cagagggcgc cgggccactc gtcgtcgcca cgcagcaggg cgcgcgccag
    11161 gttccggtag aaggagacga cggccggctc cttggggctg aggtggccgc gcgcggcgaa
    11221 ccgggagccc acggaccgct gcacggcctc caggccgcgc cggtcctccg cgttgaccat
    11281 cgcggagtac gactcgatgc cctgccgcat ctccttccgt tcggcctcac cgaggtcctt
    11341 gacccgggcg gcggggacca gcatgccccc gcggacctgg gtgcggtcga gggacaacgg
    11401 gagcatgctc agccagatca ccgcctcccc ggcggcggcg aggctcccgg tggggaagac
    11461 gaagaagtcg tacaggtgag ccttctcgtc ctcggtcagg gagaggaggg acggctgctg
    11521 gggctcggtg aacgggatgc ggaaacgcag cgcccacggc gtgtcgtcgc gcagatccgt
    11581 gtcggagcgg cccggggtgg acggttccag ggtctcgggg tggaagccca tcacgtggta
    11641 gttctcgtgc gcgttctcca cggcgatctt ccagttgcag tgccactcct cggcccattc
    11701 cccggccagt accatgtcgt ccaggcggta gttgaccagg tcgctttcga ttcgggcgag
    11761 gtgcggagcc agggcttccg ccgcggggtc gaggttgacg aagacgaacc cgtgccactc
    11821 ttccacggcg aactgcggca gtcggcagcc ggcggggtcg aacgcctcgt tgcgccgcat
    11881 gaaggtggcg ccgatcaacc ggccgtccag tccgtagcgc cacaggtggt acgggcaggt
    11941 gaaccggtcg gtgcggccgg tgcccggttc gacgaccggc atcatgcggt ggcggcagat
    12001 cggtgacatc gcgtgcagcg cgccgtcgtc gccgcgcgtg atgacgacgg gttcgcccgc
    12061 gacggtgagg gcgacgtagg caccggggcc ggcgagttgg tcggtgtgcg cgacgagaag
    12121 ccaggaacgg ccgaagacct tctcccgctc cagctcgtac agctcaccgg aggcgaacgc
    12181 ccggggcggc aggctgaggg cgggcgggtc cgggaccagg tagccaccga gttcggcgag
    12241 caggtccgcg gtctccggcg acagaggggg tgtggtggag gagtccggcc gcggggccgc
    12301 cgggccggtc tgatggaggg tcaagatccg tgtccttgct gtggaagggg ataccgggcg
    12361 ggggcgggct gtcgggtctg cggtcggcgc agcaggggga agcggtcgcc ggtgaggggg
    12421 gcgccctcgc gcagcgggtc ggctccggtg ccggggaggt cgtcgagaaa cgcgatgccg
    12481 gtcggccggt aggcggcggt ggcgtccatg tagacggcgg aggtcgacaa gcggggctcg
    12541 tcggtcgagt tcgccccggc gctgtgcacg gtgcgggcgt ggtggaaggt gcagtcgccg
    12601 gcccgcagtg gcaccgtgac ccgcggcatc cagatct
//
