BGC0000254 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000254 (Streptomyces rimosus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T2PKS	1	25,222	High	oxytetracycline	PKS

BGC0000254 - Region 1 - T2PKS

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Location: 1 - 25,222 nt. (total: 25,222 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 25,223
Label		BGC0000254

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

oxyE: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

oxyJ: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

oxyL: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

oxyM: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

oxyS: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: acylase aminotransferase hydrolase methyltransferase

oxyD: Amination

MITE: MITE0000131Amination (identity: 60.10%)
smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

oxyF: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

oxyP: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

oxyQ: aminotransferase

smcogs: SMCOG1019: aminotransferase

oxyT: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Hydrolases: hydrolase

oxyD: Amination

MITE: MITE0000131Amination (identity: 60.10%)
smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Lyases: cyclase

oxyK: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

Ligases: synthase

oxyA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

oxyB: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000254.5	1.00	PKS	oxytetracycline	Streptomyces rimosus
BGC0000231.5	0.71	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.67	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001276.3	0.63	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.62	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001164.3	0.62	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000272.3	0.61	PKS	spore pigment	Streptomyces collinus
BGC0001723.5	0.55	PKS	oviedomycin	Streptomyces antibioticus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000254.5	1.00	PKS	oxytetracycline	Streptomyces rimosus
BGC0000231.5	0.71	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.67	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001276.3	0.63	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.62	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001164.3	0.62	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000272.3	0.61	PKS	spore pigment	Streptomyces collinus
BGC0001723.5	0.55	PKS	oviedomycin	Streptomyces antibioticus

Similar known clusters from MIBiG 4.0

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

oxyP: mal

PKS_AT (22..298): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 47.1
Methylmalonyl-CoA: 39.2
Methoxymalonyl-CoA: 29.2
Propionyl-CoA: 25.8
Ethylmalonyl-CoA: 16.0
inactive: 14.2
2-Methylbutyryl-CoA: 12.2
CHC-CoA: 11.2
trans-1,2-CPDA: 9.0
Isobutyryl-CoA: 6.7
fatty_acid: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 25222
Starter unit(s):: malonamyl-CoA (Score: 1234.0; E-value: 0)
propionyl-CoA (Score: 363.0; E-value: 1.71e-128)
Malonyl elongations:: 8|9 (Score: 685.7; E-value: 3.9e-209)
Product class(es):: anthracycline
aureolic acid
tetracycline
Product molecular weight(s):: malonamyl-CoA_8: 440 Da
malonamyl-CoA_9: 482 Da
propionyl-CoA_8: 411 Da
propionyl-CoA_9: 453 Da

oxyA: KS (Score: 732.6; E-value: 2.9e-223)
oxyB: CLF 8|9 (Score: 685.7; E-value: 3.9e-209)
oxyC: ACP (Score: 120.4; E-value: 5.5e-38)
oxyD: AMID (Score: 1221.6; E-value: 0.0); AMID ELQ83270.1_Oxytetracycline_malonamyl-CoA (Score: 1234.0; E-value: 0.0)
oxyE: OXY (Score: 229.0; E-value: 2.8e-70)
oxyF: MET C6|C8 (Score: 569.9; E-value: 3.3e-174)
oxyI: CYC C1-C18|C2-C19 (Score: 236.1; E-value: 2.4e-73)
oxyJ: KR C9 (Score: 444.8; E-value: 2.1e-136)
oxyK: CYC C7-C12 (Score: 451.3; E-value: 3.1e-138)
oxyL: OXY (Score: 449.7; E-value: 3.2e-137)
oxyM: KR (Score: 136.8; E-value: 2.4e-42)
oxyN: CYC C5-C14/C3-C16 (Score: 452.8; E-value: 7.5e-139)
oxyP: AT (Score: 408.0; E-value: 6.9e-125); AT BAB72049.1_Aklavinone_propionyl-CoA (Score: 363.0; E-value: 1.71e-128)
oxyQ: AMIN (Score: 626.1; E-value: 4e-191)
oxyS: OXY (Score: 502.8; E-value: 2.4e-153)
oxyT: MET C2O|C2N (Score: 591.0; E-value: 1.5e-180)