LOCUS       BGC0000265             12970 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Streptomyces purpurascens rhodomycin gene cluster, partial sequence.
ACCESSION   BGC0000265
VERSION     BGC0000265
KEYWORDS    .
SOURCE      Streptomyces purpurascens
  ORGANISM  Streptomyces purpurascens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     On Oct 29, 2001 this sequence version replaced U10405.1.
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 13:17:04
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12970
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            <1..432
                     /gene="rdmA"
     CDS             <1..432
                     /codon_start=1
                     /gene="rdmA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF07366"
                     /gene_kind="biosynthetic-additional"
                     /note="putative methylaclanonic acid cyclase; similar to
                     translation product encoded by GenBank Accession Number
                     L13453 between nucleotides 1-158"
                     /product="RdmA"
                     /protein_id="AAA83420.1"
                     /sec_met_domain="PF07366 (E-value: 1.2e-33, bitscore:
                     105.3, seeds: 29, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIDIVRRMVNSYNTGKTDDVAEFIHEEYLNPGALEHMPELRGPE
                     AFALAVKWLKLTFSEDAHLEEISYEEKGSWVRAKLALYGRQVGELVGMPATGRKFSGEQ
                     IHLIRIVDGKIRDHRDWPDYLGTYRQLGEPWPTEEGWRP"
     source          1..12970
                     /clone="EB3"
                     /db_xref="ATCC:25489"
                     /db_xref="taxon:1924"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces purpurascens"
                     /strain="ATCC 25489"
                     /type_material="type strain of Streptomyces purpurascens"
     subregion       1..12970
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="PKS"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12970
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     regulatory      464..468
                     /regulatory_class="ribosome_binding_site"
     gene            474..1598
                     /gene="rdmB"
     CDS             474..1598
                     /codon_start=1
                     /function="possible methyltransferase"
                     /gene="rdmB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042:O-methyltransferase (Score: 243; E-value: 8e-74)"
                     /gene_kind="biosynthetic-additional"
                     /note="10-hydroxylase"
                     /product="RdmB"
                     /protein_id="AAA83421.1"
                     /transl_table=11
                     /translation="MSSSSPGEPLEPTDQDLDVLLKNLGNLVTPMALRVAATLRLVDHL
                     LAGADTLAGLADRTDTHPQALSRLVRHLTVVGVLEGGEKQGRPLRPTRLGMLLADGHPA
                     QQRAWLDLNGAVSHADLAFTGLLDVVRTGRPAYAGRYGRPFWEDLSADVALADSFDALM
                     SCDEDLAYEAPADAYDWSAVRHVLDVGGGNGGMLAAIALRAPHLRGTLVELAGPAERAR
                     RRFADAGLADRVTVAEGDFFKPLPVTADVVLLSFVLLNWSDEDALTILRGCVRALEPGG
                     RLLVLDRADVEGDGADRFFSTLLDLRMLTFMGGRVRTRDEVVDLAGSAGLALASERTSG
                     STTLPFDFSILEFTAVSEEAAPAAQASEALPAQE"
     regulatory      1591..1595
                     /gene="rdmB"
                     /regulatory_class="ribosome_binding_site"
     gene            1603..2499
                     /gene="rdmC"
     CDS             1603..2499
                     /codon_start=1
                     /function="possible C-C hydrolase"
                     /gene="rdmC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036:alpha/beta hydrolase fold protein (Score: 122.9;
                     E-value: 3.2e-37)"
                     /gene_kind="biosynthetic-additional"
                     /note="methyl estherase"
                     /product="RdmC"
                     /protein_id="AAA83422.1"
                     /sec_met_domain="PF00561 (E-value: 5.1e-21, bitscore: 65.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.2e-14, bitscore:
                     45.2, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSERIVPSGDVELWSDDFGDPADPALLLVMGGNLSALGWPDEFAR
                     RLADGGLHVIRYDHRDTGRSTTRDFAAHPYGFGELAADAVAVLDGWGVDRAHVVGLSMG
                     ATITQVIALDHHDRLSSLTMLLGGGLDIDFDANIERVMRGEPTLDGLPGPQQPFLDALA
                     LMNQPAEGRAAEVAKRVSKWRILSGTGVPFDDAEYARWEERAIDHAGGVLAEPYAHYSL
                     TLPPPSRAAELREVTVPTLVIQAEHDPIAPAPHGKHLAGLIPTARLAEIPGMGHALPSS
                     VHGPLAEVILAHTRSAA"
     regulatory      2523..2526
                     /regulatory_class="ribosome_binding_site"
     gene            2532..3245
                     /gene="rdmD"
     CDS             2532..3245
                     /codon_start=1
                     /gene="rdmD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248:methyltransferase (Score: 211.4; E-value:
                     2.5e-64)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative dimethyltransferase; required for
                     modification on 10-position of aklavinone"
                     /product="RdmD"
                     /protein_id="AAA83423.1"
                     /transl_table=11
                     /translation="MYGADLARVYDLVHRERGKDYRARDRGGRRRGPAEQAGAGRLLDV
                     ACGTGGHLRHFADLFAHVEGVELSEPMAEEARAALPGVTVHAGDMRDFRLGTTFDVVTC
                     MFGSVGYMTSVAELGRALRMFARHLEPGGVAVVDPWWFYETFADGHVSADIVTVDGVTV
                     SRVSHSARRGRTSHMDVHFVVAEPGAGAQHFVDTHIISLFSRSEYEQAFRDAGFAVEYL
                     PEAPSGRGLFVGVRG"
     regulatory      3304..3310
                     /regulatory_class="ribosome_binding_site"
     gene            3317..4924
                     /gene="rdmE"
     CDS             3317..4924
                     /codon_start=1
                     /function="putative aklavinone-11-hydroxylase"
                     /gene="rdmE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050:monooxygenase FAD-binding (Score: 361.5; E-value:
                     1.3e-109)"
                     /gene_kind="biosynthetic-additional"
                     /note="11-hydroxylase"
                     /product="RdmE"
                     /protein_id="AAA83424.1"
                     /sec_met_domain="DAO (E-value: 5.5e-11, bitscore: 32.2,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDHEVDVLVVGAGLGGLSTAMFLARQGVRVLVVERRPGLSPYPR
                     AAGQNPRTMELLRIGGVADEVVRADDIRGTQGDFVIRLAESVRGEILRTVSESFDDMVA
                     ATEPCTPAGWAMLSQDKLEPILLAQARKHGGAIRFGTRLLSFRQHDDDAGAGVTARLAG
                     PDGEYDLRAGYLVGADGNRSLVRESLGIGRYGHGTLTHMVGVIFDADLSGIMEPGTTGW
                     YYLHHPEFKGTFGPTDRPDRHTLFVEYDPDEGERPEDFTPQRCVELIGLALDAPEVKPE
                     LVDIQGWEMAARIAERWREGRVFLAGDAAKVTPPTGGMSGNAAVADGFDLAWKLAAVLQ
                     GQAGAGLLDTYEDERKVAAELVVAEALAIYAQRMAPHMAEVWDKSVGYPETLLGFRYRS
                     SAVLATDDDPARVENPLTPSGRPGFRGPHVLVSRHGERLSTVDLFGDGWTLLAGELGAD
                     WVAAAEAVSAELGVPVRAYRVGAGLTDPESAVSERYGIGKAGASLVRPDGIVAWRTDEA
                     AADAAQTLEGVLRRVLDR"
     gene            complement(5030..6070)
                     /gene="rdmF"
     CDS             complement(5030..6070)
                     /codon_start=1
                     /gene="rdmF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1079:oxidoreductase (Score: 239.3; E-value: 1.2e-72)"
                     /gene_kind="biosynthetic-additional"
                     /product="RdmF"
                     /protein_id="AAA83425.2"
                     /transl_table=11
                     /translation="MPEHRQQRALRMGVIGTANIAIRRIMPVLAAHDHVDLVAVASRDK
                     ARAERVGAAFGCGGVGDYAALVERDDLDAVYIPLPPGMHHEWALRALRSGKHVLVEKPM
                     SDTYEKTLELMSTASELGLVLAENFMFLHHSQHAAVRAMLDESVGELRLFSGSFAVPPL
                     APESFRYQPALGGGALLDVGVYPLRAAQLYLTGELDVLGACLRVDETTGADVAGSVLLS
                     DDRGVTAQLDFGFEHSYRSTYALWGNRGRVSVRRAFTPPEQLKPVVRIEQQDVVTERSL
                     PEDNQVFNAMDAFVRAALTNTGPLTDTSAIQRQALLLDRVRRAARLIGDPKSRPHDVLA
                     QDTGLS"
     gene            6233..7648
                     /gene="rdmG"
     CDS             6233..7648
                     /codon_start=1
                     /gene="rdmG"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 48; E-value: 1.5e-14)"
                     /gene_kind="biosynthetic-additional"
                     /note="putatively involved in biosynthesis of rhodosamine"
                     /product="RdmG"
                     /protein_id="AAL24449.1"
                     /transl_table=11
                     /translation="MTAATNALGRELQLVHAAQWLYAINAEPFVPGEGDPYARLVRGFE
                     SGENGTDDAGGTLASIAERGPVWRSRLDTWVVTGPAALRELSAGAEPAVSAAVPQAEAW
                     LPDPERQRTTGLARDLARRGGTRAVGRLAGSLREQAGQAAARLGPGPVDLVADFAEPLT
                     AAGYAAVLDVPEERRAAFAADLAAAAPAVDALLCPQTLDTTRRLAAGVRGLRELFAGRA
                     HAEADLLLAVAGARAAADLLGDALRRLLDRPREWALLRSDPAHAARLVEEVLHQRPPWR
                     ACPLVALATAEVAGTRIEAGDSLTAITTAAAVPGGPSGIAGPTGTAAADGASRTAGGTV
                     PPGTAGISRTAGATVPAGAAGAAGTGAAGGVGTAETTHTGRVAGTGGAGEPGAVTVLGE
                     ATRTNGVPTPLPVAACATLLPAARALAEHALVALAARFPLLRPDGEPVRTRRAPLTRRL
                     VRLPARLGEEETR"
     gene            7645..8931
                     /gene="rdmH"
     CDS             7645..8931
                     /codon_start=1
                     /gene="rdmH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MGT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1062:glycosyltransferase, MGT family (Score: 354.1;
                     E-value: 1.9e-107)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative glycosyltransferase"
                     /product="RdmH"
                     /protein_id="AAL24450.1"
                     /sec_met_domain="MGT (E-value: 3.9e-136, bitscore: 443.9,
                     seeds: 193, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLFTSLAHHTHYYPLVPLAWALRTAGHEVRIASQPELTDVITG
                     TGLTAVPVEWSLGALEDLGLLGKLHDEASAHVQGFDYAARGEHPWSYEQLLALENIMVP
                     TLYGALNNDTMVDALVDFARSWQPDLVVWETFTLAGAIAARVTGAAHCRLVSGPDISVR
                     ARKEFLRLAAEQEPEHREDPTAEWLERTLRRLGSDAGFDEDMVTGQWTVNTTPPSTQLD
                     LKLPTVQVRHVPYNGPAVVPDWLRADPGRSRVCVTLGSSASDVGVDMAELLAHLAELDA
                     EIVATVDEATREAAGDLPDNVRLVDFVPLNDLLPTCDAIVHHGGVGTKATAELHAVPQL
                     ILAFGWDTEVMGRGLEDIGAGLCVPSADLLPDVLRKQVQRLVGDPSFARGARRLQEELL
                     AVPSPNEVVPVLERLVAEHGTHGSVHDRG"
     gene            8943..10244
                     /gene="rdmI"
     CDS             8943..10244
                     /codon_start=1
                     /gene="rdmI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056:DegT/DnrJ/EryC1/StrS aminotransferase (Score:
                     357.6; E-value: 1.4e-108)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative hexose-C-3-dehydratase"
                     /product="RdmI"
                     /protein_id="AAL24451.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.2e-98,
                     bitscore: 320.3, seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 4.7e-08, bitscore:
                     21.9, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDTKALVLELARKYHKEQETRGFEPGVTPILSSGAVLDEEDRGA
                     LVEAALEMRIAAGVRTRKFESEFARYFRLRKAHMVNSGSSANLLAVSALTSPKLKDLRL
                     RPGDEAITVGAGFPTTINPLVQNGLTPVFVDVELGTYNASLAQIEAAISPRTRLIMIAH
                     ALGNPFPVAEVAELARRHDLFFIEDNCDAVGSTYQGRLTGTFGDLSTVSFYPAHHITAG
                     EGGCVLTSSLELARIVESMRDWGRDCWCEPGTDNTCLKRFDYQLGTLPAGYDHKYIFSH
                     VGYNLKATDLQGALALSQLRKVDDFGAARRHNWQRLRDGLADVPGLLLPRATPGSDPSW
                     FGFALTVLPDAGFTRRDLVDFLEGRRIGTRRLFGGNITRHPAYEDVRYRIVDELVNCDT
                     VTEDTFWIGVYPGLTKDMLDYVVESITEFCTRKG"
     gene            10252..11046
                     /gene="rdmJ"
     CDS             10252..11046
                     /NRPS_PKS="Domain: PKS_KR (10-122). E-value: 5.6e-12.
                     Score: 37.8. Matches aSDomain:
                     nrpspksdomains_rdmJ_PKS_KR.1"
                     /codon_start=1
                     /gene="rdmJ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     265.7; E-value: 6.4e-81)"
                     /gene_kind="biosynthetic-additional"
                     /note="polyketide ketoreductase"
                     /product="RdmJ"
                     /protein_id="AAL24452.1"
                     /sec_met_domain="adh_short (E-value: 7.6e-40, bitscore:
                     126.5, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSRFTPTSRVAVVTGATSGIGLSVTRSLAAVGHRVFLCARDADR
                     VALTVKELRAEGHDVDGAACDVRDVAQIESLVSAAVERYGPVEVLVNNAGRSGGGPTHT
                     IADELWDDVMATNLDSVFRVTRAVLTTGRMREGGWGRVVNIASTGGKQGVVLGAPYSAS
                     KHGVVGFTKALGLELAKTGITVNAVCPGYVETPMAVRVRQGYAAAWETSEEEVLDRFQA
                     KIPLGRYTTPDEVAGMVGYLLTDAASSVTAQAINVCGGLGNY"
     aSDomain        10282..10617
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_rdmJ_PKS_KR.1"
                     /evalue="5.60E-12"
                     /label="rdmJ_PKS_KR.1"
                     /locus_tag="rdmJ"
                     /protein_end="122"
                     /protein_start="10"
                     /score="37.8"
                     /tool="antismash"
                     /translation="VAVVTGATSGIGLSVTRSLAAVGHRVFLCARDADRVALTVKELRA
                     EGHDVDGAACDVRDVAQIESLVSAAVERYGPVEVLVNNAGRSGGGPTHTIADELWDDVM
                     ATNLDSVF"
     CDS_motif       10288..10356
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_rdmJ_0001"
                     /evalue="9.20E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="rdmJ"
                     /protein_end="35"
                     /protein_start="12"
                     /score="18.3"
                     /tool="antismash"
                     /translation="VVTGATSGIGLSVTRSLAAVGHR"
     gene            11055..12413
                     /gene="rdmK"
     CDS             11055..12413
                     /NRPS_PKS="Domain: Polyketide_cyc2 (7-148). E-value:
                     2.9e-13. Score: 42.3. Matches aSDomain:
                     nrpspksdomains_rdmK_Polyketide_cyc2.1"
                     /NRPS_PKS="Domain: Polyketide_cyc2 (156-295). E-value:
                     2.3e-09. Score: 29.7. Matches aSDomain:
                     nrpspksdomains_rdmK_Polyketide_cyc2.2"
                     /codon_start=1
                     /gene="rdmK"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1128:cyclase/dehydrase (Score: 421.9; E-value:
                     2.6e-128)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative aromatase"
                     /product="RdmK"
                     /protein_id="AAL24453.1"
                     /transl_table=11
                     /translation="MSDETVHTTVHEIEVRAPAEAVFDLVAGASRWPVHFPPSVHVERL
                     EGDDRQERLRIWATANDEVKSWTSRRELDRAGLRVTFRQEESRAPVRSMSGEWLLEPLG
                     DKGTRVRFTHAFTAVDDAPENVEWIRRAIDTNTGSELEALRRGVEDADPELTFTFDDSV
                     RVEGSAKDAYEFIRAADRWTERLPHVARVVLTEDTPNEQILEMDTLTADGATHTTRSVR
                     VCFPAERIVYKQIRTPALMAAHTGEWIFEEDDKGCTVTSRHTVRIEPNAVTSVLGPAAD
                     VAAARTMIREALGRNSLTTLRCAADHAERLRGAEPVPAGTYVAVQQFYARQMRLLDERR
                     AEEWARTFTEDGVFAQNIAPEPLRGRDAVAAAVRRNLERTADTPEQRRDVFSMLTVVPG
                     PDGSLSTRCYAQVLVTPAGGPARLHLSAVCEDELVPHGDGWLVRHRRVEHDGV"
     aSDomain        11076..11498
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_rdmK_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="2.90E-13"
                     /label="rdmK_Polyketide_cyc2.1"
                     /locus_tag="rdmK"
                     /protein_end="148"
                     /protein_start="7"
                     /score="42.3"
                     /tool="antismash"
                     /translation="TTVHEIEVRAPAEAVFDLVAGASRWPVHFPPSVHVERLEGDDRQE
                     RLRIWATANDEVKSWTSRRELDRAGLRVTFRQEESRAPVRSMSGEWLLEPLGDKGTRVR
                     FTHAFTAVDDAPENVEWIRRAIDTNTGSELEALRRGV"
     aSModule        11076..11939
                     /domains="nrpspksdomains_rdmK_Polyketide_cyc2.1"
                     /domains="nrpspksdomains_rdmK_Polyketide_cyc2.2"
                     /incomplete
                     /locus_tags="rdmK"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        11523..11939
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_rdmK_Polyketide_cyc2.2"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="2.30E-09"
                     /label="rdmK_Polyketide_cyc2.2"
                     /locus_tag="rdmK"
                     /protein_end="295"
                     /protein_start="156"
                     /score="29.7"
                     /tool="antismash"
                     /translation="FTFDDSVRVEGSAKDAYEFIRAADRWTERLPHVARVVLTEDTPNE
                     QILEMDTLTADGATHTTRSVRVCFPAERIVYKQIRTPALMAAHTGEWIFEEDDKGCTVT
                     SRHTVRIEPNAVTSVLGPAADVAAARTMIREALGR"
     CDS             complement(12889..>12970)
                     /codon_start=2
                     /gene="rdmL"
                     /note="putative C-7 ketoreductase"
                     /product="RdmL"
                     /protein_id="AAL24454.1"
                     /transl_table=11
                     /translation="MTALADPEGRYMTGAVVPVDGGLSLT"
     gene            complement(12889..>12970)
                     /gene="rdmL"
ORIGIN
        1 gatcgaatcg acattgttcg ccgcatggtg aacagctaca acaccggaaa aacggatgac
       61 gtggccgagt tcatccacga ggagtacctc aatcccggtg ccctggaaca catgccggaa
      121 ctgcgcggtc ccgaggcgtt cgcactcgcg gtgaagtggc tgaagctcac tttctccgag
      181 gacgcgcatc tggaggagat ctcctacgag gagaaaggca gctgggtgcg ggcgaaactc
      241 gccctgtacg gacgtcaggt gggtgaactc gtaggaatgc ccgccaccgg gcgcaagttc
      301 tccggcgaac agattcacct catccggatc gtcgacggaa agatccgcga ccaccgcgac
      361 tggcccgact atctcggcac ctaccgccag ctcggcgagc cgtggcccac cgaggagggc
      421 tggcgcccct gagcagcccg tccggcacac cgacgcccaa gggaaggaaa acggtgagct
      481 cttcctcacc cggggaaccc ctggaaccga ccgaccagga cctcgacgta ctcctgaaga
      541 acctcggcaa tctggtgacc ccgatggccc tgagggtcgc cgcgacactc cgcctggtcg
      601 atcacctgct ggccggagcc gacaccctcg ccggcctcgc cgaccgtacg gacacccatc
      661 cccaggcgct ctcccgcctg gtccggcacc tgaccgtcgt cggcgtcctg gagggcggcg
      721 agaagcaggg ccggccgctg cgccccaccc ggctcgggat gctcctcgcc gacggccacc
      781 cggcccagca gcgggcctgg ctggacctga acggggccgt ctcccacgcc gacctggcct
      841 tcaccgggct cctcgacgtg gtccgcaccg gccgccccgc ctacgccggg cggtacgggc
      901 ggcccttctg ggaggacctc tcggcggacg tggcgctggc cgactccttc gacgcgctca
      961 tgtcctgcga cgaggacctg gcctacgagg ccccggccga cgcgtacgac tggtcggcgg
     1021 tccggcacgt cctcgacgtg ggcggcggca acggcggcat gctcgcggcg atcgccctgc
     1081 gggccccgca cctgcgcggc accctggtcg aactggccgg tcccgccgag cgcgcccggc
     1141 gcaggttcgc ggacgccggt ctggccgacc gggtcacggt ggcggagggc gacttcttca
     1201 agccgctgcc cgtcaccgcc gacgtcgtcc tgctgtcgtt cgtcctgctc aactggtccg
     1261 acgaggacgc gctgacgatc ctgcgcggct gcgtcagggc gctggagccg ggaggcaggc
     1321 tcctggtcct cgaccgtgcc gatgtcgagg gggacggcgc cgaccggttc ttcagcacgc
     1381 tgctcgacct gcggatgctg acgttcatgg gcggacgggt gcgcacccgg gacgaggtcg
     1441 tggacctggc cggatcggcc ggactcgccc tggcctcgga gcgcacgagc ggctccacga
     1501 ccctcccgtt cgacttctcg atcctcgagt tcacggccgt ctccgaggaa gcggccccgg
     1561 ccgcccaggc ctccgaagcc ctcccagcac aggagtagac acatgtccga acgcatcgtg
     1621 ccgagcgggg acgtcgagct gtggagcgac gacttcggtg acccggcgga cccggcgctg
     1681 ctgctcgtca tgggcggcaa cctctccgcc ctcggctggc ccgacgagtt cgcccggcgg
     1741 ctcgccgacg gcggcctcca cgtcatccgc tacgaccacc gcgacaccgg ccgctccacc
     1801 acccgcgact tcgccgcgca cccctacggc ttcggcgagc tggccgccga cgcggtcgcc
     1861 gtcctcgacg gctggggcgt cgaccgggcc catgtcgtcg gcctctcgat gggggcgacc
     1921 atcacccagg tcatcgccct cgaccaccac gaccggctga gcagcctgac catgctgctc
     1981 ggcggcgggc tcgacatcga cttcgacgcc aacatcgagc gcgtgatgcg cggcgaaccc
     2041 acgctggacg gcctgcccgg gccccagcag ccgttcctgg acgcgctcgc cctgatgaac
     2101 cagccggccg agggacgcgc cgccgaggtg gccaagcgcg tgagcaagtg gcgcatcctc
     2161 tccggcacgg gtgtgccgtt cgacgacgcc gagtacgcgc ggtgggagga acgggcgatc
     2221 gaccacgcgg ggggcgtcct cgccgagccg tacgcgcact actcgctgac cctgccgccg
     2281 ccgtcccggg ccgccgagct gcgcgaggtc accgtgccga ccctggtcat ccaggccgag
     2341 cacgacccca tcgcccccgc gccgcacggc aagcacctcg ccgggctcat ccccaccgcg
     2401 cgcctcgcgg agatcccggg catggggcac gccctgcctt cctcggtcca cgggccgctc
     2461 gccgaggtca tcctcgccca cacccgctcg gcggcctgac cgtggcggac gagcagcggg
     2521 cgggagagac gatgtacggg gccgacctcg cccgggtcta cgacctggtg caccgggagc
     2581 ggggcaagga ctaccgggcc cgagaccgag gcggtcgccg ccgcggcccg gcggagcagg
     2641 ccggcgccgg acggctgctc gacgtcgcgt gcggcaccgg aggccatctg cggcacttcg
     2701 ccgacctctt cgcccacgtc gagggagtgg agctgtcgga gccgatggcc gaggaggcac
     2761 gggccgcgct gcccggggtg accgtgcacg cgggcgacat gcgcgacttc cgcctcggca
     2821 ccaccttcga cgtcgtgacc tgcatgttcg gctccgtcgg ctatatgacg tcggtcgccg
     2881 aactcggccg ggcgctgagg atgttcgccc ggcatctgga gccgggcggg gtggccgtgg
     2941 tcgacccgtg gtggttctac gagacgttcg ccgacggcca tgtctccgcc gacatcgtga
     3001 ccgtcgacgg cgtcaccgtc tcccgggtct cgcactccgc ccgccggggc cgtacctcgc
     3061 acatggacgt gcacttcgtg gtcgccgaac ccggcgccgg cgcccagcac ttcgtcgaca
     3121 cccacatcat ctccctgttc agccgctccg agtacgaaca ggccttccgc gacgccggct
     3181 tcgcggtcga gtacctgccc gaggcgccgt cgggccgcgg cctgttcgtc ggcgtccggg
     3241 ggtgaaccga gcgacgggac cccgcgagaa acgacgagaa cgacgagagc gacgagagcg
     3301 acgagagagg aacgacatga acgaccacga ggtcgatgtt ctggtggtgg gtgcgggcct
     3361 cgggggcctg tcgacggcga tgttcctcgc ccggcagggc gtccgcgtgc tcgtggtgga
     3421 gcgcaggccg ggcctctcgc cctatccgcg ggccgccggg cagaacccgc ggaccatgga
     3481 gctgctgcgc atcgggggcg tcgcggacga ggtggtccgg gccgacgaca tccgcggcac
     3541 ccagggcgac ttcgtcatcc ggctcgccga gagcgtgcgg ggcgagatcc tgcggaccgt
     3601 ctcggagagc ttcgacgaca tggtggccgc caccgagccg tgcacaccgg ccggctgggc
     3661 gatgctgtcc caggacaagc tggagccgat cctgctcgcg caggcgcgca agcacggtgg
     3721 cgccatccgc ttcggcaccc ggctgctgtc gttccgccag cacgacgacg acgccggcgc
     3781 cggcgtcacg gcccggctgg ccgggccgga cggcgagtac gacctgcgcg ccgggtatct
     3841 cgtgggcgcc gacggcaacc gcagcctggt gcgggagtcg ctgggcatcg ggcggtacgg
     3901 gcacggcacg ctgacccaca tggtcggggt catcttcgac gcggacctct ccggcatcat
     3961 ggagccgggc acgaccggct ggtactacct ccaccacccg gagttcaagg gcacgttcgg
     4021 gcccacggac cggcccgacc ggcacaccct cttcgtcgag tacgaccccg acgagggcga
     4081 gcgccccgag gacttcacac cgcagcgctg cgtcgaactc atcggcctcg ccctggacgc
     4141 ccccgaggtg aagcccgagc tcgtcgacat ccagggctgg gagatggcgg cccgcatcgc
     4201 cgaacgctgg cgcgaggggc gggtgttcct ggccggggac gcggcgaagg tcaccccgcc
     4261 gaccgggggc atgagcggca acgcggccgt cgccgacggg ttcgacctgg cctggaagct
     4321 cgcggcggtg ctgcaggggc aggccggggc gggcctgctc gacacctacg aggacgagcg
     4381 caaggtcgcc gcggagctgg tcgtggccga ggcgctggcg atctacgcgc agcggatggc
     4441 gccgcacatg gcggaggtgt gggacaagtc ggtggggtac cccgagacgc tgctcggctt
     4501 ccgctaccgc tcctcggcgg tcctggccac ggacgacgac ccggcgcggg tggagaatcc
     4561 gctgacgccg agcgggcgtc ccggcttccg ggggccgcat gtcctggtgt cgcggcacgg
     4621 ggagcggctc tccacggtcg atctgttcgg tgacgggtgg acgttgctgg cgggggagct
     4681 gggggcggac tgggtcgcgg cggcggaggc ggtctctgcg gaactgggag tgcctgtgcg
     4741 ggcgtaccgg gtcggggcgg ggctgactga tcccgagagc gcggtgtccg agcggtacgg
     4801 catcgggaag gcgggggcga gtctggtgcg gccggacggg attgtggcgt ggcgtacgga
     4861 tgaggcggcg gctgatgcgg ctcagactct cgaaggggtt ctgcgtaggg ttcttgaccg
     4921 ctgaaggaat ggcttcgcgc cccgccggag gattggcggg gcgcggtgtc gtcggcggct
     4981 gcgggcgggt ggggggtgtt cgcgcagttc cccgcgcccc tgactgcgtt cagctgagcc
     5041 ctgtgtcctg cgcgaggacg tcatgcgggc gggacttcgg gtcgcctatg agacgggccg
     5101 ctcggcggac ccggtcgagc agcagggcct ggcgctggat cgccgaggtg tcggtgaggg
     5161 ggccggtgtt cgtgagggcc gcgcggacga aggcgtccat cgcgttgaag acctggttgt
     5221 cctcgggcag cgaccgctcc gtcaccacgt cctgctgctc gatcctgacc accggcttca
     5281 gctgctccgg cggtgtgaag gcacgccgga cgctcacccg cccccggttg ccccacagcg
     5341 cgtaggtcga ccggtaggag tgctcgaagc cgaagtccag ctgggccgtc actccccggt
     5401 cgtcgctcag cagcacactg cccgccacat cggccccggt cgtctcgtcg acgcgcagac
     5461 acgccccgag gacgtcgagt tccccggtga gatacagctg ggccgcccgc agcggataca
     5521 ccccgacgtc gagcaacgcg cccccgccca gggccggctg gtaccggaac gactcgggtg
     5581 ccagcggcgg cacggcgaac gacccggaga agagccgcag ttctcccacg gactcgtcga
     5641 gcatcgcgcg taccgccgcg tgctgggaat ggtgcaggaa catgaagttc tcggcgagca
     5701 cgagtccgag ttccgacgcg gtcgacatca gctcgagagt tttctcgtac gtgtccgaca
     5761 tcggtttctc gaccagcacg tgctttcccg aacgcaaagc ccgcagcgcc cactcgtgat
     5821 gcatgccggg cggcagcgga atatagacgg cgtcaaggtc gtcccgctcg acgagcgccg
     5881 cgtaatcccc cacgccaccg cagccgaaag cggcccccac ccgctcggcc cgggccttgt
     5941 cccggctggc caccgcgaca aggtcgacgt ggtcatgcgc ggcgagcacg ggcatgatcc
     6001 ggcgaatcgc gatgttcgcc gtgccgatca cgcccatgcg gagcgcccgt tgctgacggt
     6061 gctctggcat agggatcctc atcaccttgc gggagtccag tgggttcccc accgtacgac
     6121 cgggagatca agcactgctt gaggaacggt ggatacggaa aacggcagtt cgagtgttct
     6181 tctaattcgg ccccgtacct tgccgaggta atcggatgac ggaggtggcg gggtgacggc
     6241 ggcgacgaat gccctggggc gcgaactgca actcgtccac gcggcacagt ggctctacgc
     6301 gatcaacgcg gagccgttcg taccggggga gggtgacccg tatgcccggc tggtacgggg
     6361 gttcgagagc ggcgagaacg gcacggacga cgcgggggga accctcgcgt cgatcgcgga
     6421 gcgggggccg gtgtggcgca gccggctcga cacctgggtg gtcaccggcc ccgcggcgct
     6481 gcgggagttg tccgccggcg cggaaccggc ggtgtccgcc gcggtgccgc aggcggaggc
     6541 ctggctgccc gacccggagc ggcagcgcac gacgggactc gcccgggacc tggcgcggcg
     6601 gggcgggacg agggccgtgg ggaggctggc cggatcgctg cgggagcagg ccgggcaggc
     6661 cgcggcccgg ctcggcccgg gtccggtcga cctggtggcc gacttcgccg agccgctgac
     6721 cgcggccggg tacgccgcgg tcctggacgt ccccgaggag cggcgcgcgg cgttcgccgc
     6781 cgacctcgcc gcggcggccc cggccgtgga cgcgctgctc tgcccgcaga cgctggacac
     6841 gacgcggcgc ctggcggcag gcgtacgcgg actccgggaa ctcttcgccg ggcgcgctca
     6901 cgcggaggcg gacctgctgc tcgcggtggc cggggcccgg gccgcggcgg acctgctcgg
     6961 cgacgcgctg cgcaggctcc tcgaccggcc gcgggagtgg gcgctgctgc gctcggaccc
     7021 cgcgcacgct gcccgtctgg tcgaggaggt actgcaccag cggccgccgt ggcgggcgtg
     7081 ccccctggtg gccctcgcca cggccgaggt ggccggcacc cgtatcgagg ccggtgacag
     7141 cctgaccgcc atcacgaccg ccgccgccgt gccgggtggg cccagcggca tcgctgggcc
     7201 caccgggacc gccgcggccg acggggccag ccggaccgcc gggggcaccg tgccccccgg
     7261 caccgccggg atcagtcgga ccgccggggc cacagtgccc gccggagccg caggagccgc
     7321 cgggaccggg gccgccgggg gcgtcggcac cgccgagacc acccacaccg gccgggtcgc
     7381 cggcaccggc ggtgccggcg aacccggcgc ggtcaccgtg ctcggcgagg ccaccaggac
     7441 caacggcgtc ccgacgcccc ttcccgtggc cgcctgcgcc accctgctcc cggccgcgcg
     7501 ggcgctcgcc gagcacgcgc tggtggcgct ggccgcgcgg ttcccgctgc tgcggccgga
     7561 cggcgagccg gtgcgcacgc gccgggcgcc cctgacccgg cgcctggtca ggctgccggc
     7621 ccgactcggc gaggaggaga cgcgatgagg gttctgttca cctcactggc ccaccacacc
     7681 cactactacc cgctcgtacc gctcgcctgg gcgctgcgca ccgcgggcca cgaggtgcgg
     7741 atcgccagcc agcccgagct gaccgacgtc atcacgggta cgggactgac ggccgtcccc
     7801 gtggagtggt cgctcggcgc cctggaggac ctcgggctgc tcggcaagct gcacgacgag
     7861 gcctcggccc atgtgcaggg cttcgactac gcagcccggg gcgaacaccc ctggagctac
     7921 gagcagttgc tggcactgga gaacatcatg gtgcccacgc tctacggcgc gctcaacaac
     7981 gacaccatgg tcgacgcgct cgtggacttc gcccgctcct ggcagcccga cctcgtcgtc
     8041 tgggagacct tcaccctcgc cggggccatc gcggcccggg tgacgggcgc ggcgcactgc
     8101 cggctggtct cgggccccga catcagcgtg cgggcccgca aggagttcct gcggctggcc
     8161 gccgagcagg agccggagca ccgcgaggac cccaccgccg agtggctgga gcgcacgctg
     8221 cggcggctgg gatccgacgc cgggttcgac gaggacatgg tcaccgggca gtggacggtc
     8281 aacaccacac cgcccagcac ccagctcgac ctgaagctgc cgaccgtgca ggtgcgccac
     8341 gtgccgtaca acggccccgc ggtggtgccg gactggctgc gggccgaccc cgggcgcagc
     8401 cgcgtctgtg tgacgctcgg ctcctcggcc tccgacgtgg gcgtcgacat ggccgagctc
     8461 ctggcccacc tcgcggagct ggacgcggag atcgtcgcga ccgtcgacga ggcgacccgc
     8521 gaggccgccg gtgacctgcc ggacaacgta cgcctggtcg acttcgtccc gctcaacgac
     8581 ctgctgccca cgtgcgacgc gatcgtgcac cacggcggtg tcggcaccaa ggcgacggcc
     8641 gaactgcacg ccgtgcccca gctgatcctg gccttcggct gggacaccga ggtcatgggg
     8701 cgcggactgg aggacatcgg cgccggcctg tgcgtgccga gcgccgacct gctgccggac
     8761 gtcctgcgca agcaggtcca gcggctggtc ggcgacccgt cgttcgcacg tggcgcccgc
     8821 aggcttcagg aggaactgct cgcggtgccc agtcccaacg aggtcgttcc ggtgctggaa
     8881 cggctggtcg ccgagcacgg cacgcacggc tcagtccacg accgagggtg agggatccag
     8941 acgtgacgga caccaaggcg ctggtgctgg aactggcgcg caagtaccac aaggaacagg
     9001 aaacgcgcgg gttcgaaccc ggagtcacgc cgatcctgtc ctcgggcgcg gtcctcgacg
     9061 aggaggaccg gggcgccctg gtcgaggcgg cgctggagat gcggatcgcc gccggggtgc
     9121 gcacccgcaa gttcgagagc gagttcgccc gctacttccg gctgcgcaag gcccacatgg
     9181 tgaactcggg atcgtcggcc aacctgctgg cggtcagcgc gctcacctcg ccgaagctga
     9241 aggacctgcg gctgcgcccc ggcgacgagg ccatcacggt gggcgccggc ttcccgacca
     9301 cgatcaaccc gctcgtccag aacggcctga caccggtctt cgtggacgtg gaactgggca
     9361 cctacaacgc ctccctggcc cagatcgagg cggcgatctc cccgcgcacc aggctcatca
     9421 tgatcgcgca cgccctgggc aacccgttcc ccgtcgccga ggtcgcggaa ctggcccgcc
     9481 ggcacgatct gttcttcatc gaggacaact gcgacgccgt cggctccacc taccagggca
     9541 ggctcaccgg caccttcggc gacctgtcga cggtgagctt ctacccggcc caccacatca
     9601 cggccggtga gggcggctgc gtcctcacgt cgagtctgga actggcccgg atcgtcgagt
     9661 cgatgcgcga ctggggccgg gactgctggt gcgaaccggg cacggacaac acctgcctga
     9721 agcggttcga ctaccagctc gggaccctcc cggccggcta cgaccacaag tacatcttct
     9781 cccacgtcgg ctacaacctg aaggccaccg acctccaagg cgccctggca ctgagccagt
     9841 tgaggaaggt cgacgacttc ggcgcggccc ggcggcacaa ctggcagcgg ctgcgggacg
     9901 gtctggccga tgtgcccgga ctgctgctgc cgcgggccac ccccggcagc gacccgagct
     9961 ggttcggctt cgccctgacc gtgctgcccg acgcgggctt cacccgcagg gacctcgtcg
    10021 acttcctgga gggccgccgg atcggcaccc gccggctgtt cggcgggaac atcacccgcc
    10081 atcccgccta cgaggacgtg cgctaccgga tcgtggacga gctggtcaac tgcgacaccg
    10141 tcacggagga caccttctgg atcggcgtct atccggggct caccaaagac atgctcgact
    10201 acgtcgtcga gtccatcacc gagttctgca cgcgaaaggg ctgatcggac gatgtcctcg
    10261 aggttcacac caaccagccg ggtcgccgtc gtcaccggcg ccaccagcgg gatcggcctc
    10321 tccgtcacac ggtccctggc cgccgtcggc caccgcgtgt tcctctgcgc ccgggacgcc
    10381 gaccgggtcg cgctcacggt caaggagctg cgcgccgagg gccacgacgt ggacggcgcc
    10441 gcctgcgacg tacgggacgt ggcccagatc gagtcgctgg tgtccgcggc ggtggagcgc
    10501 tacgggcccg tcgaggtgct cgtcaacaac gccggccgca gcggcggcgg ccccacgcac
    10561 accatcgccg acgagctgtg ggacgacgtg atggccacca acctggacag cgtcttccgc
    10621 gtcacccgtg ccgtcctgac caccggccgc atgcgggagg gcggctgggg ccgggtcgtg
    10681 aacatcgcct ccaccggcgg caagcagggc gtggtgctgg gcgcccccta ctccgcctcc
    10741 aagcacgggg tcgtcggctt caccaaggcc ctggggctgg aactggccaa gaccgggatc
    10801 acggtcaacg cggtgtgccc cggctatgtg gagacgccca tggcggtgcg ggtccggcag
    10861 ggctacgccg ccgcctggga gacctccgag gaggaggtgc tcgaccggtt ccaggccaag
    10921 atcccgctcg gccgctacac cacgcccgac gaggtggcgg gcatggtcgg ctacctgctg
    10981 accgacgcgg catcgtccgt caccgcgcag gcgatcaacg tctgcggcgg gctgggaaac
    11041 tactgaggag agccatgtcg gacgagaccg tgcacaccac cgtccacgag atcgaggtac
    11101 gggccccggc cgaggcggtg ttcgacctgg tcgccggggc ctcccgctgg ccggtgcact
    11161 tcccgccgag cgtgcacgtc gagcgcctgg agggcgacga ccggcaggag cggctgagga
    11221 tctgggcgac ggccaacgac gaggtcaaga gctggacctc gcgccgcgaa ctggaccggg
    11281 ccggattacg ggtgacgttc cggcaggagg agtcgcgggc gccggtgcgg agcatgagcg
    11341 gcgagtggct gctggagccg ctcggcgaca aggggacccg ggtgcggttc acccacgcgt
    11401 tcaccgccgt ggacgacgcc cccgagaacg tcgagtggat ccgccgggcc atcgacacca
    11461 acaccgggtc cgaactcgag gccctgcgcc ggggcgtgga ggacgcggac ccggagctca
    11521 cgttcacgtt cgacgacagt gtgcgcgtcg agggctcggc gaaggacgcg tacgagttca
    11581 tccgcgcggc cgaccgctgg acggagcggc tgccgcacgt cgcccgggtc gtcctcaccg
    11641 aggacacccc gaacgagcag atcctggaga tggacacgct caccgccgac ggcgcgaccc
    11701 acaccacccg ttcggtgcgc gtctgcttcc ccgccgagcg gatcgtctac aagcagatcc
    11761 ggacacccgc gctgatggcc gcccacaccg gcgaatggat cttcgaggag gacgacaagg
    11821 gctgcaccgt cacctcccgg cacaccgtgc ggatcgagcc gaacgccgtg acgagcgtcc
    11881 tcggtccggc ggcggacgtg gccgccgccc gcaccatgat ccgggaggcg ctcggccgca
    11941 acagcctcac gacgctgcgg tgcgcggccg accacgccga gcggctgcgc ggcgccgagc
    12001 cggtccccgc cgggacgtac gtcgccgtgc agcagttcta cgcccggcag atgcggctcc
    12061 tcgacgagcg gcgggccgag gagtgggccc ggacgttcac cgaggacggc gtcttcgcgc
    12121 agaacatcgc ccccgagccc ctgcgcggcc gggacgccgt ggccgcggcc gtacgccgca
    12181 acctggagcg gacggcggac acaccggagc agcggcggga tgtgttctcc atgctcaccg
    12241 tggtgcccgg gccggacggt tccctgagca cccgctgcta cgcacaggtc ctggtcaccc
    12301 cggccggggg accggccagg ctgcacctga gcgcggtgtg cgaggacgaa ctggtgccgc
    12361 acggcgacgg ctggctggtg cgccaccgca gggtcgagca cgacggagtc tgagccgccg
    12421 ccggccgggc acacacgacg acgggccgca ccggttgcag gtgcggcccg tcggcgtgcg
    12481 gtcggaggtc cgtgcggccc gactgcaggt gccgcccggc tgtccgtgga gcccgggcgg
    12541 cggcgagacc ccggcgcccg cgcggcacgt gacggcggcc cgggtttggg tgaggctccg
    12601 gcgctcgcat ggaggtgtcg gcggcccgga cctcggtgtg gcccggcggc gggcggtccg
    12661 gggccccgtg tggcccggcg ggtgcggtcc ggccatcttc cggcccggac acggagagcc
    12721 cggcaggcct tgcggcccgg gccctccgtg tggtccggcg gcgggcggtc cgtccctccg
    12781 tccggcccgg acacggcggg cctggccgag cttgcggccc gggcgctccg tgtggtccgg
    12841 cggcgtgcgg tccggctctc cgtgcccccc gggggcccgg gggagcggtc aggtcaggga
    12901 gagaccgccg tcgaccggga cgaccgcgcc cgtcatgtag cggccttcgg ggtcggccag
    12961 cgcggtgatc
//
