BGC0000269 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000269 (Streptomyces sp. SF2575)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T2PKS,PKS-like	1	53,273	High	SF2575	PKS:Type II+saccharide:hybrid/tailoring

BGC0000269 - Region 1 - PKS-like,T2PKS

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Location: 1 - 53,273 nt. (total: 53,273 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)
PKS-like: (t2pks2 or ksIII)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 53,274
Label		BGC0000269

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

ssfF: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

ssfK: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

ssfO1: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

ssfO2: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

ssfP: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

ssfS4: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

ssfU: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase aminotransferase glycosyltransferase hydrolase kinase methyltransferase transferase

ADE34480.1: aminotransferase class V

smcogs: SMCOG1139: aminotransferase class V

ssfD: Amination

MITE: MITE0000131Amination (identity: 62.50%)
smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

ssfE: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

ssfM1: Methylation

MITE: MITE0000023Methylation (identity: 100.00%)
smcogs: SMCOG1042: O-methyltransferase

ssfM2: Methylation

MITE: MITE0000021Methylation (identity: 100.00%)
smcogs: SMCOG1042: O-methyltransferase

ssfM3: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

ssfM4: Methylation

MITE: MITE0000022Methylation (identity: 100.00%)
smcogs: SMCOG1042: O-methyltransferase

ssfS1: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

ssfS6: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

ssfV: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

ssfX2: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

Hydrolases: hydrolase

ssfD: Amination

MITE: MITE0000131Amination (identity: 62.50%)
smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Lyases: cyclase dehydratase hydratase

ssfJ: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

ssfS2: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

ssfS5: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

ssfY1: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

ssfY3: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

Isomerases: isomerase

ssfH: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

ssfA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ssfB: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ssfG: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

ssfL1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ssfL2: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ssfN: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000269.5	1.47	PKS, saccharide	SF2575	Streptomyces sp. SF2575
BGC0000231.5	0.64	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001254.3	0.53	PKS	ACT-Toxin II	Alternaria alternata
BGC0000234.5	0.52	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001135.5	0.51	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000278.3	0.48	PKS	urdamycin	Streptomyces fradiae
BGC0001513.3	0.44	PKS	anthrabenzoxocinone	Actinobacteria bacterium
BGC0000218.5	0.42	PKS	doxorubicin	Streptomyces peucetius
BGC0001723.5	0.42	PKS	oviedomycin	Streptomyces antibioticus
BGC0000273.5	0.42	PKS, saccharide	steffimycin D	Streptomyces steffisburgensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000269.5	0.99	PKS, saccharide	SF2575	Streptomyces sp. SF2575
BGC0000231.5	0.69	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001254.3	0.63	PKS	ACT-Toxin II	Alternaria alternata
BGC0000278.3	0.61	PKS	urdamycin	Streptomyces fradiae
BGC0000234.5	0.60	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002274.2	0.59	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624
BGC0001135.5	0.59	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001723.5	0.56	PKS	oviedomycin	Streptomyces antibioticus
BGC0000218.5	0.54	PKS	doxorubicin	Streptomyces peucetius
BGC0000272.3	0.54	PKS	spore pigment	Streptomyces collinus

Similar known clusters from MIBiG 4.0

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

ssfV: mal

PKS_AT (7..287): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 86.7
Methylmalonyl-CoA: 39.8
Methoxymalonyl-CoA: 35.0
Propionyl-CoA: 22.9
inactive: 17.7
Acetyl-CoA: 13.9
2-Methylbutyryl-CoA: 11.4
trans-1,2-CPDA: 10.1
CHC-CoA: 8.9
fatty_acid: 8.6
Ethylmalonyl-CoA: 8.2
Isobutyryl-CoA: 7.1
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ssfL2: X

AMP-binding (36..434): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TFMYFGGPSK
Nearest Stachelhaus code(s):: Leu DFLYFGGVVK (29% 8Å match)
Stachelhaus code match:: 60% (weak)

ssfL1: X

AMP-binding (32..447): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: PLPGQGVLNK
Nearest Stachelhaus code(s):: Sal PLPAQGVLNK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 9930 to 53273
Starter unit(s):: malonamyl-CoA (Score: 2319.0; E-value: 0)
Malonyl elongations:: 8|9 (Score: 661.0; E-value: 1.3e-201)
Product class(es):: angucycline
anthracycline
aureolic acid
tetracenomycin
tetracycline
Product molecular weight(s):: malonamyl-CoA_8: 654 Da
malonamyl-CoA_9: 696 Da

ssfA: KS (Score: 751.9; E-value: 3.9e-229)
ssfB: CLF 8|9 (Score: 661.0; E-value: 1.3e-201)
ssfC: ACP (Score: 122.0; E-value: 1.7e-38)
ssfD: AMID (Score: 1218.5; E-value: 0.0); AMID ADE34521.1_SF2575_malonamyl-CoA (Score: 1250.0; E-value: 0.0)
ssfF: KR (Score: 135.5; E-value: 6.1e-42)
ssfK: KR (Score: 181.3; E-value: 6.5e-56)
ssfL2: LIG (Score: 787.9; E-value: 8.6e-240); LIG ADE34493.1_SF2575_malonamyl-CoA (Score: 1069.0; E-value: 0.0)
ssfM1: MET C2O|C2N (Score: 412.1; E-value: 3.7e-126)
ssfM2: MET (Score: 260.4; E-value: 5.4e-80)
ssfO1: OXY (Score: 492.4; E-value: 3.6e-150)
ssfS6: GT (Score: 397.9; E-value: 1.4e-121)
ssfU: KR C9 (Score: 464.8; E-value: 1.7e-142)