BGC0000269 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000269 (Streptomyces sp. SF2575)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T2PKS,PKS-like	1	53,273	SF2575	PKS:Type II+saccharide:hybrid/tailoring	100%

BGC0000269 - Region 1 - PKS-like,T2PKS

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Location: 1 - 53,273 nt. (total: 53,273 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)
PKS-like: (t2pks2 or ksIII)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 53,274
Label		BGC0000269

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000269	1.57	Polyketide, Saccharide	SF2575	Streptomyces sp. SF2575
BGC0000272	0.83	Polyketide	spore pigment	Streptomyces collinus
BGC0000885	0.69	Other	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0002069	0.65	Other (Aminocoumarin)	styrolide A, styrolide B	Pseudomonas fluorescens
BGC0000231	0.64	Polyketide	griseusin A, griseusin B	Streptomyces griseus
BGC0002493	0.61	NRP	parabactin	Paracoccus denitrificans PD1222
BGC0000343	0.60	NRP	enterobactin	Pseudomonas sp. J465
BGC0002476	0.57	NRP	enterobactin	Escherichia coli str. K-12 substr. MG1655
BGC0000846	0.56	Other	tabtoxin	Pseudomonas syringae
BGC0000922	0.56	Other	pseudomonas quinolone signal (PQS)	Pseudomonas aeruginosa PAO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000269	1.00	Polyketide, Saccharide	SF2575	Streptomyces sp. SF2575
BGC0000272	0.73	Polyketide	spore pigment	Streptomyces collinus
BGC0000231	0.69	Polyketide	griseusin A, griseusin B	Streptomyces griseus
BGC0000885	0.67	Other	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0001896	0.67	Other	carbazomycin B	Streptomyces luteoverticillatus
BGC0002733	0.66	Other	sinapigladioside	Burkholderia gladioli
BGC0000343	0.66	NRP	enterobactin	Pseudomonas sp. J465
BGC0002069	0.66	Other (Aminocoumarin)	styrolide A, styrolide B	Pseudomonas fluorescens
BGC0001817	0.64	Other	vancosamine	Streptomyces sp. WAC2288
BGC0002493	0.64	NRP	parabactin	Paracoccus denitrificans PD1222

Similar known gene clusters from MIBiG 4.0

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

ssfV

PKS_AT (7..287): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 86.7
Methylmalonyl-CoA: 39.8
Methoxymalonyl-CoA: 35.0
Propionyl-CoA: 22.9
inactive: 17.7
Acetyl-CoA: 13.9
2-Methylbutyryl-CoA: 11.4
trans-1,2-CPDA: 10.1
CHC-CoA: 8.9
fatty_acid: 8.6
Ethylmalonyl-CoA: 8.2
Isobutyryl-CoA: 7.1
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ssfL2

AMP-binding (36..434): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TFMYFGGPSK
Nearest Stachelhaus code(s):: Leu DFLYFGGVVK (29% 8Å match)
Stachelhaus code match:: 60% (weak)

ssfL1

AMP-binding (32..447): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: PLPGQGVLNK
Nearest Stachelhaus code(s):: Sal PLPAQGVLNK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

Type II PKS gene cluster prediction details

Cluster section:: 9930 to 53273
Starter unit(s):: malonamyl-CoA (Score: 2319.0; E-value: 0)
Malonyl elongations:: 8|9 (Score: 661.0; E-value: 1.3e-201)
Product class(es):: angucycline
anthracycline
aureolic acid
tetracenomycin
tetracycline
Product molecular weight(s):: malonamyl-CoA_8: 654 Da
malonamyl-CoA_9: 696 Da

ssfA: KS (Score: 751.9; E-value: 3.9e-229)
ssfB: CLF 8|9 (Score: 661.0; E-value: 1.3e-201)
ssfC: ACP (Score: 122.0; E-value: 1.7e-38)
ssfD: AMID (Score: 1218.5; E-value: 0.0); AMID ADE34521.1_SF2575_malonamyl-CoA (Score: 1250.0; E-value: 0.0)
ssfF: KR (Score: 135.5; E-value: 6.1e-42)
ssfK: KR (Score: 181.3; E-value: 6.5e-56)
ssfL2: LIG (Score: 787.9; E-value: 8.6e-240); LIG ADE34493.1_SF2575_malonamyl-CoA (Score: 1069.0; E-value: 0.0)
ssfM1: MET C2O|C2N (Score: 412.1; E-value: 3.7e-126)
ssfM2: MET (Score: 260.4; E-value: 5.4e-80)
ssfO1: OXY (Score: 492.4; E-value: 3.6e-150)
ssfS6: GT (Score: 397.9; E-value: 1.4e-121)
ssfU: KR C9 (Score: 464.8; E-value: 1.7e-142)