Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000269.1:
Cluster 1Polyketide / Saccharide / Other153273

BGC0000269, SF2575 biosynthetic gene cluster from Streptomyces sp. SF2575. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: SF2575
PubChem ID: 54702959
SMILES string: Copy to clipboard
Molecular formula: C40H43NO15
Average molecular mass: 777.776 Da
Molecular activity: Cytotoxic

Class-specific details

Biosynthetic class(es):
Polyketide / Saccharide / Other
Polyketide subclass:
Tetracycline (cyclic)
Polyketide synthase subclass:
Type II
Starter unit:
Malonamyl-CoA
Polyketide synthase / ketosynthase-encoding genes:
ADE34518
Genes involved in folding/cyclization:
ADE34490,ADE34489,ADE34493

Saccharide subclass:
hybrid/tailoring
Glycosyltransferases:
ADE34512:
Specificity: D-olivose
Evidence for specificity: Activity assay
Sugar biosynthesis subcluster: ADE34515,ADE34516,ADE34510,ADE34511,ADE34492
Other biosynthetic class:
None

Gene cluster description

SF2575 (BGC0000269). Gene Cluster 1. Biosynthetic class = Polyketide/Saccharide/Other. GenBank GQ409537, positions 2200-49800. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays
MIxS-compliance:Unknown
Contact for this cluster:Yit-Heng Chooi (The Australian National University)

Literature references

1. Pickens LB et al. (2009) Biochemical analysis of the biosynthetic pathway of an anticancer tetracycline SF2575. J Am Chem Soc 131(48):17677-89. doi: 10.1021/ja907852c.
2. Hatsu M et al. (1992) A new tetracycline antibiotic with antitumor activity. I. Taxonomy and fermentation of the producing strain, isolation and characterization of SF2575. J Antibiot (Tokyo) 45(3):320-4.
3. Pickens LB et al. (2011) Structural and biochemical characterization of the salicylyl-acyltranferase SsfX3 from a tetracycline biosynthetic pathway. J Biol Chem 286(48):41539-51. doi: 10.1074/jbc.M111.299859. Epub 2011
4. Li L et al. (2014) C-glycosylation of anhydrotetracycline scaffold with SsfS6 from the SF2575 biosynthetic pathway. J Antibiot (Tokyo) 67(1):65-70. doi: 10.1038/ja.2013.88. Epub 2013 Sep