BGC0000270 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000270 (Streptomyces antibioticus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T2PKS,aminocoumarin	1	39,428	High	simocyclinone D8	PKS

BGC0000270 - Region 1 - T2PKS,aminocoumarin

Download region GenBank file

Download region SVG

Location: 1 - 39,428 nt. (total: 39,428 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)
aminocoumarin: (novK or novJ or novI or novH or SpcDK_cou)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 39,429
Label		BGC0000270

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

sim15: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

sim5: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

sim7: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

sim8: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

simI: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

simJ1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

simJ2: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

simK: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: PPTase transferase

sim10: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

sim11: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

sim19: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

sim23: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

Hydrolases: esterase hydrolase

AAL15614.1: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

sim13: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: cyclase dehydratase

sim24: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

sim6: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

Ligases: ligase synthase

AAL15617.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

sim3: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

simH: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

simL: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000270.5	1.72	PKS	simocyclinone D8	Streptomyces antibioticus
BGC0000231.5	0.74	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001833.4	0.69	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001072.5	0.69	PKS, other (aminocoumarin), saccharide	simocyclinone D8	Streptomyces antibioticus
BGC0001825.4	0.68	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000270.5	0.99	PKS	simocyclinone D8	Streptomyces antibioticus
BGC0000231.5	0.83	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.73	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001723.5	0.72	PKS	oviedomycin	Streptomyces antibioticus
BGC0001072.5	0.69	PKS, other (aminocoumarin), saccharide	simocyclinone D8	Streptomyces antibioticus
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.68	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Type II PKS

Predicted core structure(s)

For candidate cluster 1, location 0 - 36379:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Tyr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

simL: X

AMP-binding (15..415): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: -GAAFTVIIK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

simH: Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (413..808): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DATVVAAVCK
Nearest Stachelhaus code(s):: Phe DAWTVAAVCK (76% 8Å match); Tyr DASTVAAVCK (74% 8Å match)
Stachelhaus code match:: 80% (moderate)

AAL15617.1: X

AMP-binding (1..176): X

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 36379
Starter unit(s):: acetyl-CoA (Score: 0.0; E-value: 0)
Malonyl elongations:: 8|9 (Score: 714.3; E-value: 8.4e-218)
Product class(es):: angucycline
anthracycline
aureolic acid
tetracycline
Product molecular weight(s):: acetyl-CoA_8: 391 Da
acetyl-CoA_9: 433 Da

sim3: CLF 8|9 (Score: 714.3; E-value: 8.4e-218)
sim4: ACP (Score: 132.1; E-value: 1.3e-41)
sim5: KR C9 (Score: 487.6; E-value: 1.9e-149)
sim6: CYC C7-C12 (Score: 499.6; E-value: 6e-153)
sim7: OXY (Score: 589.4; E-value: 1.3e-179)
sim8: KR C15 (Score: 487.4; E-value: 2e-149)
sim9: KR (Score: 42.0; E-value: 2e-13)
simJ1: KR (Score: 210.4; E-value: 8.5e-65)
simJ2: KR (Score: 206.4; E-value: 1.4e-63)
simK: KR (Score: 83.2; E-value: 5.5e-26)