LOCUS       BGC0000271             11118 bp    DNA     linear   BCT 11-OCT-2001
DEFINITION  Streptomyces avermitilis spore-pigment biosynthetic gene cluster.
ACCESSION   BGC0000271
VERSION     BGC0000271
KEYWORDS    .
SOURCE      Streptomyces avermitilis
  ORGANISM  Streptomyces avermitilis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:17:54
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11118
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..510)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135:MarR family
                     transcriptional regulator (Score: 99.3; E-value: 2.2e-30)"
                     /gene_kind="regulatory"
                     /product="regulator protein"
                     /protein_id="BAB69161.1"
                     /transl_table=11
                     /translation="MPKPLSLAFDPIARADEHWKQRWGNVPSMAAITSIMRAHQILLAE
                     VDAVVKPYGLTFARYEALVLLTFSKAGELPMSKIGERLMVHPTSVTNTVDRLVTSGLVD
                     KRPNPNDGRGTLASITDKGREVCDAATRDLMAMDFGLGAYDGEECAEIFAMLRPLRIAA
                     HDFDEE"
     source          1..11118
                     /db_xref="taxon:33903"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces avermitilis"
     protocluster    1..11118
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[4393:6909]"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_product_classes="pentangular polyphenol"
                     /t2pks_product_classes="tetracenomycin"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
                     /tool="antismash"
     proto_core      4394..6909
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_product_classes="pentangular polyphenol"
                     /t2pks_product_classes="tetracenomycin"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
     cand_cluster    1..11118
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(t2ks and t2clf)"
                     /kind="single"
                     /product="T2PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..11118
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000271"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11118
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T2PKS"
                     /region_number="1"
                     /rules="(t2ks and t2clf)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             complement(760..2349)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050:monooxygenase FAD-binding (Score: 516.7; E-value:
                     1.2e-156)"
                     /gene_functions="biosynthetic-additional (t2pks) OXY
                     (Score: 259.2; E-value: 1.9e-79)"
                     /gene_kind="biosynthetic-additional"
                     /product="hydroxylase"
                     /protein_id="BAB69162.1"
                     /transl_table=11
                     /translation="MGLSASLFLGRLGIPHMLVEKHAGTSKHPRGRGNNVRTMELFRVA
                     GAEQAIRKAASTLADNHGILQTSSLTDDEGEWLFKEIDPGGGLARFSPSGWCLCSQNDL
                     EPVLLKSARGLGGDIRFSTEMLSFDQDAEGVTALMKNRDTGAHTTVRAEYLIAADGPRS
                     PVRERLGIRQTGPGDLFHNVSITFNSRALADAVGDRRFICCYLTNPEADGALLPVDNVE
                     KWVFHAPWHPEQGETLEDFTDERCVSHIRRAVGDLDMDVEITGRAPWHAAERVAERYAS
                     ERVFLAGDSAHEMSPTGAFGSNTGIQDAHNLAWKLAAVLNGWAGPGLLQSYEAERLPVA
                     QATSARASARSAEHSHPGYAPAPGAGGGGGGRQGGVLKVALGYRYPRGAVIGADPAGPV
                     VPEQMAMAGEPGSRAPHMWLLRSGARTSTLDLYERSFVLLSGAAEDAWHRAATSVAERL
                     AVSLDSYRIGKDPHAELVPENGTDWAELHGTTPRGAVLVRPDGYVAWRSEGPLAEPEAT
                     LYEIMASLLDLA"
     CDS             2756..3880
                     /codon_start=1
                     /product="WhiE I homolog"
                     /protein_id="BAB69163.1"
                     /transl_table=11
                     /translation="MTTSERVSQSAFDGSRLRVVLLLDLHDGAQKQFLEAYEQLRNQVA
                     SVPGHISDQLCQSIENPSQWLITSEWESAPPFLAWVNSEEHVETVQPLHSCVRDTRSLR
                     FSVLRETGNISAPLPQSEKGRLQATARVGDGVVRHALTFTVKPGSEPMVAKILAGYSSP
                     QAQVDENTRLRRTSLFMHGNRVVRAIEVEGDLMAALRHVSRQPEVMAVEEAINPYLEQD
                     RDLSDPGSARVFFTRAALPAVHHVSVGTGEPSDVERHALFYPAKSGSGMALARLLARED
                     EAAADDPANPIHCSTIFQRDDIVVRLVDVRGSIEDDPVLALGLKGDRNGAVLSRLLDAD
                     ALGVDGAPTTEKEISRFLERSDMQLITDRSSSAS"
     CDS             3954..4397
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MannoseP_isomer"
                     /gene_functions="biosynthetic-additional (t2pks) CYC C5-C18
                     (Score: 215.2; E-value: 5.5e-67)"
                     /gene_kind="biosynthetic-additional"
                     /product="WhiE II homolog"
                     /protein_id="BAB69164.1"
                     /sec_met_domain="MannoseP_isomer (E-value: 3.7e-09,
                     bitscore: 26.3, seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPRPRIVDLSETEPNTRRGGDLRAMLTPTAVGAKSGFMGLAIV
                     QPGERIGEHYHPYSEEFVYVVSGRLEVDLDGETHPIQPDQGLLIPINMRHRFRNVGDVE
                     ARMVFHLGPLAPRPKLGHVDTEETDGLEGAGQAMTPQRTEAVS"
     CDS             4394..5659
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (4-414). E-value:
                     1.2e-54. Score: 177.6. Matches aSDomain:
                     nrpspksdomains_BAB69165.1_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2ks"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 471.8; E-value:
                     3.3e-143)"
                     /gene_functions="biosynthetic-additional (t2pks) KS (Score:
                     724.1; E-value: 1e-220)"
                     /gene_kind="biosynthetic"
                     /product="3-oxoacyl-(acyl carrier protein) synthase I"
                     /protein_id="BAB69165.1"
                     /sec_met_domain="t2ks (E-value: 4.6e-212, bitscore: 694.1,
                     seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRRVAVTGVGIVAPGGVGVPAFWDLLANGRTATRGITLFNPEGL
                     RSRIAAECDFDPAAHGIDAAAAERADRYVQFAMVAAREAVGDAGLGPEKEDPWRIGVSL
                     GTAVGGTTRLEHDYVAVSETGRRWDVDHRLADPHLHRAFSPSTLASEVAEQFGAHGPVQ
                     TVSTGCTSGLDAIGYAFHAVQEGRVDVCIAGASDSPISPITMACFDAIKATSPNNDDPE
                     HASKPFDKHRNGFVMGEGGAVLILEELEHARARGARVYCEIGGYATFGNAYHMTGLTRE
                     GLEMARAIDGALAHARLDGSDIDYVNAHGSGTQQNDRHETAAVKRSLGDHAYRTPMSSI
                     KSMVGHSLGAIGAIEVLACVLALVHQVVPPTANYTTPDPECDLDYVPRTARQVKLRNVL
                     SVGSGFGGFQSAVVLTRPDGRT"
     aSDomain        4406..5635
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BAB69165.1_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="1.20E-54"
                     /label="BAB69165.1_PKS_KS.1"
                     /locus_tag="BAB69165.1"
                     /protein_end="414"
                     /protein_start="4"
                     /score="177.6"
                     /tool="antismash"
                     /translation="VAVTGVGIVAPGGVGVPAFWDLLANGRTATRGITLFNPEGLRSRI
                     AAECDFDPAAHGIDAAAAERADRYVQFAMVAAREAVGDAGLGPEKEDPWRIGVSLGTAV
                     GGTTRLEHDYVAVSETGRRWDVDHRLADPHLHRAFSPSTLASEVAEQFGAHGPVQTVST
                     GCTSGLDAIGYAFHAVQEGRVDVCIAGASDSPISPITMACFDAIKATSPNNDDPEHASK
                     PFDKHRNGFVMGEGGAVLILEELEHARARGARVYCEIGGYATFGNAYHMTGLTREGLEM
                     ARAIDGALAHARLDGSDIDYVNAHGSGTQQNDRHETAAVKRSLGDHAYRTPMSSIKSMV
                     GHSLGAIGAIEVLACVLALVHQVVPPTANYTTPDPECDLDYVPRTARQVKLRNVLSVGS
                     GFGGFQSAVVL"
     CDS             5659..6909
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (141-409). E-value:
                     2.7e-26. Score: 84.3. Matches aSDomain:
                     nrpspksdomains_BAB69166.1_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2clf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093:Beta-ketoacyl synthase (Score: 589.4; E-value:
                     4.8e-179)"
                     /gene_functions="biosynthetic-additional (t2pks) CLF
                     (Score: 555.0; E-value: 2.2e-169)"
                     /gene_kind="biosynthetic"
                     /product="chain length factor"
                     /protein_id="BAB69166.1"
                     /sec_met_domain="t2clf (E-value: 9.7e-144, bitscore: 468.6,
                     seeds: 26, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPQQKRRAAITGIGVVAPNGLSTETFWKSTEKGLCVLDRVTREG
                     CDHLPLRIAGEVRGFDPVALIEERFLVQTDRFTHFAMAAADLALEDARLGRTYTGDEPF
                     GIGVVTAAGSGGGEFGQRELQRLWGQGPRFVGPYQSIAWFYAASTGQISIRGGFKGPCG
                     VVASDEAGGLDALAHAARAVRRGTDVVVAGAAEAPLAPYSMVCQLGYEDLSTSDDPACA
                     YRPFTGAACGFVPAEGGAMLVVEEETVARGREAAIRAFVAGHGATFTGASCWAQSREGL
                     AHAIRDALNEARCAPEEIDVVFADAFGVPEADRAEALAIADVLGAQGRRVPVTAPKTGI
                     GRAYCGAPVLDVAAAVLAMEHGLVPPTPNVFDPCYDLDLVTGSARPAELRTALVLSRGL
                     MGSNAALVLRHGAHALS"
     aSDomain        6082..6885
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BAB69166.1_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="2.70E-26"
                     /label="BAB69166.1_PKS_KS.1"
                     /locus_tag="BAB69166.1"
                     /protein_end="409"
                     /protein_start="141"
                     /score="84.3"
                     /tool="antismash"
                     /translation="AWFYAASTGQISIRGGFKGPCGVVASDEAGGLDALAHAARAVRRG
                     TDVVVAGAAEAPLAPYSMVCQLGYEDLSTSDDPACAYRPFTGAACGFVPAEGGAMLVVE
                     EETVARGREAAIRAFVAGHGATFTGASCWAQSREGLAHAIRDALNEARCAPEEIDVVFA
                     DAFGVPEADRAEALAIADVLGAQGRRVPVTAPKTGIGRAYCGAPVLDVAAAVLAMEHGL
                     VPPTPNVFDPCYDLDLVTGSARPAELRTALVLSRGLMGSNAALVLR"
     CDS             6896..7180
                     /NRPS_PKS="Domain: PP-binding (17-85). E-value: 2.7e-09.
                     Score: 29.3. Matches aSDomain:
                     nrpspksdomains_BAB69167.1_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1129:acyl carrier protein (Score: 86.1; E-value:
                     2.2e-26)"
                     /gene_functions="biosynthetic-additional (t2pks) ACP
                     (Score: 76.7; E-value: 2.5e-24)"
                     /gene_kind="biosynthetic-additional"
                     /product="acyl carrier protein"
                     /protein_id="BAB69167.1"
                     /sec_met_domain="PP-binding (E-value: 5.8e-10, bitscore:
                     29.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSHEKEKEMDNQLTVQELAALMKKAAGVTVDVKELEDRLDSGFA
                     EFGLDSLGLLGIVGELENGHGAVLPPDAERCRNPREFLDLVNNNIVAGA"
     aSDomain        6947..7150
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BAB69167.1_PP-binding.1"
                     /evalue="2.70E-09"
                     /label="BAB69167.1_PP-binding.1"
                     /locus_tag="BAB69167.1"
                     /protein_end="85"
                     /protein_start="17"
                     /score="29.3"
                     /tool="antismash"
                     /translation="ELAALMKKAAGVTVDVKELEDRLDSGFAEFGLDSLGLLGIVGELE
                     NGHGAVLPPDAERCRNPREFLDL"
     CDS             7182..7661
                     /NRPS_PKS="Domain: Polyketide_cyc (10-122). E-value:
                     8.2e-17. Score: 53.5. Matches aSDomain:
                     nrpspksdomains_BAB69168.1_Polyketide_cyc.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1128:cyclase/dehydrase (Score: 220.5; E-value:
                     5.4e-67)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC C9-C14
                     (Score: 253.7; E-value: 1.1e-78)"
                     /gene_kind="biosynthetic-additional"
                     /product="cyclase I"
                     /protein_id="BAB69168.1"
                     /transl_table=11
                     /translation="MSGHTENDIIIAAPLDLVWDMTNDIESWPQLFSEYASVDVLSREN
                     GTTTFRLTMHPDENGKVWSWVSERTPDREKLTVTARRVETGPFEYMNIRWVYSQTPDGT
                     RMHWTQDFAMKPEAPVDDAWMTDNINRNSVVQMELIRDKVEQHARERRTASVTTD"
     aSDomain        7212..7547
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BAB69168.1_Polyketide_cyc.1"
                     /domain_subtypes="Polyketide_cyc"
                     /evalue="8.20E-17"
                     /label="BAB69168.1_Polyketide_cyc.1"
                     /locus_tag="BAB69168.1"
                     /protein_end="122"
                     /protein_start="10"
                     /score="53.5"
                     /tool="antismash"
                     /translation="IAAPLDLVWDMTNDIESWPQLFSEYASVDVLSRENGTTTFRLTMH
                     PDENGKVWSWVSERTPDREKLTVTARRVETGPFEYMNIRWVYSQTPDGTRMHWTQDFAM
                     KPEAPVDD"
     CDS             7741..8067
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1225:Polyketide synthesis cyclase (Score: 162.8;
                     E-value: 3.6e-50)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC
                     C4-C21/C2-C23|C2-C19 (Score: 163.5; E-value: 3e-51)"
                     /gene_kind="biosynthetic-additional"
                     /product="cyclase II"
                     /protein_id="BAB69169.1"
                     /transl_table=11
                     /translation="MHQALIVARMAPGSAPDIAKVFAESDSGELPHLVGVTRRTLFQFN
                     DVYMHLVEADRPPGPAIAEVAGHPAFRDISDRLSTYVSAYDPTTWRSPKDAMAQEFYRW
                     ERES"
     CDS             complement(8101..9096)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042:O-methyltransferase (Score: 305; E-value:
                     1.1e-92)"
                     /gene_functions="biosynthetic-additional (t2pks) MET C6|C8
                     (Score: 521.6; E-value: 1.6e-159)"
                     /gene_kind="biosynthetic-additional"
                     /product="O-methyltransferase"
                     /protein_id="BAB69170.1"
                     /transl_table=11
                     /translation="MQLRELVFGAACAAAVRAAAKLGVADALGEKPATAEELAAVLGTE
                     PRPLLRLLRALSCYGIFAQNDDGRFVHTSMSRLLREDDPHSLRYISLWCTEPWTWDAWP
                     RLDEAVRSGGSVFHELYGKGFFDYLHEDAHDSAQVFNQAMTTSSEQSARDVAELLDLTG
                     VSTVADIGGGQGHVLASLLEKHPDIRGTLLDLPRVVEKADPRLRDGGPLAARARIVGAD
                     CREEIPVEADLYIIKNILEWDDDSTRRTLRNVMTKARPGARVVIIENLVDDTPSMKFTT
                     SMDLMLLLNVGGAKHTKESLVTRMTEAGLTVGEIRPVNAYLHAFECTVPA"
     CDS             complement(9182..9994)
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="BAB69171.1"
                     /transl_table=11
                     /translation="MPAATRAADKCAAAGNGICVEGTSAHPVTRTSVRSLTLTGYKKNG
                     LWASNTDKLTVRDVAADKNKNWGIAIERSVRSVLEENVARGNAESGVFLSNTVDTEAGA
                     TDTRGTVVRHNRLEGNRIGLTVRRLRNVTVEENELSANCSGLFAVGDEGKPHAGALAIR
                     GNGVHENNKYCAKTARLPYIQGSGIVLTGTEDTVVEYNDIRDNVGKSVFSGGVVLYKSF
                     VGTPNKRNVIRDNVLSGNSTADLANRDTGTGNKFEHNSCQRSEPAGMC"
     CDS             complement(10486..11118)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057:TetR family
                     transcriptional regulator (Score: 79.2; E-value: 9e-24)"
                     /gene_kind="regulatory"
                     /product="regulator protein"
                     /protein_id="BAB69172.1"
                     /transl_table=11
                     /translation="MSETTKTKLLEGALRALSEQGIAKTSARAIAAAAGVNQALVFYHF
                     GSVDELLAAACRHGAEQVVARYRARLDEVATLAELLALGRELHEQEKAEGHVAMLGQLL
                     AGAQTQPRLAPATAAGLDLWIAEIEKVLTRVLTPTPLGEFADPAGLARAVAASFVGIEL
                     YEGVDAEGAGAALAALDQLALLLTALEDLGPVAQRAVRHTLRRATRG"
ORIGIN
        1 ctactcctcg tcgaagtcgt gcgcggcgat gcgcagcggg cgcagcatcg cgaagatctc
       61 cgcgcactcc tccccgtcgt aggcaccgag cccgaagtcc atcgccatca ggtcgcgggt
      121 cgccgcgtcg cagacctcgc ggcccttgtc ggtgatcgag gcgagggttc cgcgcccgtc
      181 gttcgggttg gggcgcttgt cgacgagccc ggacgtcacc agccggtcca cggtgttggt
      241 gacggacgtc gggtgcacca tcaaccgctc gccgatcttg gacatcggca gctcgcccgc
      301 cttggagaag gtgagcagca ccagcgcctc gtaccgcgcg aaggtcagcc cgtacggctt
      361 gacgaccgcg tcgacctccg ccagcagaat ctggtgcgcg cgcatgatcg aggtgatcgc
      421 ggccatggac ggcacgtttc cccagcgctg cttccagtgt tcgtcggcgc gcgcgatggg
      481 atcgaaggcg agactgaggg gcttcggcac ggcaatagac cttaccggcc ggtcatatcg
      541 cggtcagccc cgtctcgccc ttcggtcgcc gcccgtccgt acgcccggcc gccccggccg
      601 gcagttgcgc ggcgggccgg cccccgcgcc cccgcgcgcg agactcgaac gcctcgcgcg
      661 cgggggcgcg gccggcaccg ccgtactcac cgcgctcgac gagcccgcca ccacggcggt
      721 gcccgccgag cgcccgcggc cggcggcgtg tgcgatccct caggccaggt ccagcaggga
      781 ggccatgatc tcgtacagcg tcgcctccgg ttcggccagc gggccctcgg accgccacgc
      841 cacatacccg tcgggccgca ccagcaccgc gccccgcggc gtcgtgccgt ggagttcggc
      901 ccagtccgtg ccgttctcgg ggacgagctc ggcgtgcggg tccttgccga tgcggtacga
      961 gtcgaggctt acggcgaggc gctcggcaac gctggtcgcc gcccggtgcc aggcgtcctc
     1021 ggcagccccg ctgagcagga cgaacgagcg ctcgtacaga tcgagcgtgg aggtgcgggc
     1081 gccggagcgc aggagccaca tgtgcggggc gcggctgccg ggttcgccgg ccatggccat
     1141 ctgctcgggc acgaccgggc ccgccgggtc ggcgccgatg acggcgccac gcggatagcg
     1201 gtagcccagg gccaccttga ggacaccgcc ctgacgtccg ccgccaccgc cgcccgcgcc
     1261 gggagcgggg gcgtagcccg gatggctgtg ttccgcggaa cgggccgagg cccgcgcgct
     1321 cgtcgcctgc gccactggga gacgctcggc ctcgtaggac tggagcagcc cgggcccggc
     1381 ccacccgttc agtacggcgg cgagcttcca ggcgaggttg tgtgcgtcct ggataccggt
     1441 gttggagccg aacgccccgg tcggcgacat ctcgtgggcg gagtccccgg cgaggaagac
     1501 ccgttccgac gcgtaccgct cggcgacccg ctccgccgcg tgccagggcg ccctgccggt
     1561 gatctcgacg tccatgtcga gatcaccgac ggcccggcgg atgtgggaga cgcaccgctc
     1621 gtcggtgaag tcctccagcg tctcgccctg ctcggggtgc cagggagcgt ggaacaccca
     1681 cttctccacg ttgtccacgg gcaggagcgc gccgtccgcc tccgggttcg tcaggtagca
     1741 gcagatgaag cgccggtcgc ccaccgcgtc cgcgagtgcg cgggagttga aggtgatgct
     1801 cacgttgtgg aacaggtctc cggggcccgt ctggcggatg cccaggcgct cgcggaccgg
     1861 gctgcgcggc ccgtcggccg cgatgaggta ctccgcccgc accgtggtgt gcgcgccggt
     1921 gtcccggttc ttcatgagcg cggtgacgcc ctccgcgtcc tggtcgaagg agagcatctc
     1981 ggtggagaac cggatgtcgc ccccgaggcc gcgggcggac ttcagcagca ccggctccag
     2041 gtcgttctga ctgcacagac accatccgct cgggctgaag cgggccaggc cgccacccgg
     2101 gtcgatctcc ttgaacagcc actcgccctc gtcgtcggtc agggacgagg tctgcaggat
     2161 gccgtggttg tccgccaggg tcgacgcggc cttgcggatc gcttgttcgg ccccggccac
     2221 ccggaacagc tccatcgtgc gcacgttgtt cccgcgccca cgcgggtgct tcgaggtgcc
     2281 cgcgtgtttc tcgaccagca tgtgcgggat gccgagccgg ccgaggaaca gcgaggcgga
     2341 cagacccacc agggatcccc ccacgatgag aaccggcacg tggtggtcga cttcttcgtt
     2401 catcaatgcc tccagcagcg gaccccggct acagggcttc catggacccc cggctacagg
     2461 gagtccatgc cccgacggga ggggttcgct cgccccattc acccggctgg ttcagcctct
     2521 gttccacccg cttcacccgt gcggtttcac ccgcttcacc cgtgcggtca cccacgtggt
     2581 tcacgcgact cgcgcgatgc tccgggccga cccacgatcg ggatccgggc tgatcgccgt
     2641 ccccgacacc gggggcgtac ccgggcatcc ggcgatcggc cctgtgaatc cacgacgcct
     2701 gacgctgctt cacaaggctt ggcgcccggg ctgtccctaa ggagatgggt gctggatgac
     2761 aacttcggaa cgtgtatcgc agtccgcgtt cgacggctcc cggctgcggg tcgtcctgct
     2821 gctggacctc catgacggcg cccagaagca gttcctggag gcctacgaac aactgcgcaa
     2881 ccaagtggcc tccgtgccgg gccacatcag cgaccagctg tgccagtcga tcgagaatcc
     2941 ctcgcagtgg ctcatcacca gcgagtggga gagcgccccg cccttcctgg cgtgggtgaa
     3001 cagcgaggaa cacgtcgaga cggtccagcc gctgcacagc tgcgtccggg acacgcggtc
     3061 cctgcgcttc agcgttctgc gcgagacggg gaacatctcc gcgccgctcc cccagtcgga
     3121 gaagggccgc ctccaggcga cggcacgggt gggcgacggc gtcgtgcgcc acgccctcac
     3181 cttcaccgtg aagccgggca gcgagccgat ggtcgccaag atcctcgccg gatactcctc
     3241 gccgcaggcg caggtcgacg agaacacccg actgcgccgc acctcgctct tcatgcacgg
     3301 caaccgggtg gtacgcgcca tcgaggtgga aggcgacctg atggccgccc tgcgccatgt
     3361 gtcccggcag cccgaggtca tggcggtcga ggaggccatc aacccctatc tggagcagga
     3421 ccgggacctc agcgatccgg gatcggcccg ggtcttcttc acccgggcgg cgctgcccgc
     3481 ggtgcaccat gtgtcggtcg gcaccggcga gccgtcggac gtggagcggc acgcgctgtt
     3541 ctacccggcc aagagcgggt ccggcatggc cctggcccgg ctgctcgccc gcgaggacga
     3601 ggcggcggcc gacgaccccg ccaaccccat ccactgcagc accatcttcc agcgcgacga
     3661 catcgtggtc cgtctggtcg atgtgcgggg cagcatcgag gacgaccccg tcctggccct
     3721 cggtctcaag ggcgaccgca acggcgcggt cctgtcccgc ctgctcgacg ccgacgcgct
     3781 cggcgtcgac ggtgcgccga cgaccgagaa ggagatctcg cgcttcctcg agcggtccga
     3841 catgcaactg atcaccgacc gcagctcatc agcctcctga ccggagcgtc cggcccggtc
     3901 cgcggccgga cgcgcactac gccgtcggca cacatcaact ggaggaaccg cacatgacca
     3961 cgccccgccc acgcatcgtg gacctcagcg agaccgagcc caacaccagg cgcggaggtg
     4021 atctgcgcgc catgctcacg cccaccgcgg tgggcgccaa gagcggcttc atgggcctcg
     4081 ccatcgtgca gcccggcgaa cgcatcggcg agcactacca cccgtactcc gaggagttcg
     4141 tctacgtcgt gagcggtcgg ctcgaagtcg acctggacgg cgagacgcac ccgatccagc
     4201 ccgaccaggg cctgctgatt cccatcaaca tgcggcaccg cttccgcaac gtgggcgacg
     4261 tggaggcccg catggtcttc cacctgggcc cgctggcgcc gcgccccaag ctcggccacg
     4321 tcgacaccga ggagaccgac ggcctggagg gagccggaca ggcgatgaca ccacagcgaa
     4381 ccgaggccgt gtcatgaccc ggcgcgtggc ggtcaccggc gtcggcatcg tcgccccggg
     4441 cggtgtgggc gtcccggcgt tctgggacct cctggcgaac ggccgcaccg cgacgagggg
     4501 catcacgctc ttcaacccgg aaggtctgcg ttcgcgtatc gcggccgagt gcgacttcga
     4561 cccggccgcc cacggaatcg acgcggcggc cgctgagcgc gccgaccgct atgtccagtt
     4621 cgccatggtc gccgccaggg aggcggtggg cgacgcggga ctcggcccgg agaaggaaga
     4681 cccctggcgc atcggggtgt ccctgggcac cgccgtcggt ggcaccacgc gcctcgaaca
     4741 cgactatgtc gcggtgagcg agaccggccg gcggtgggat gtcgaccacc gcctggccga
     4801 cccccatctg caccgcgcgt tctcacccag caccctcgcc tccgaggtgg ccgaacagtt
     4861 cggtgcccac ggcccggtgc agaccgtctc cacgggctgc acctccggcc tcgacgcgat
     4921 cggctacgcc ttccacgccg tacaggaggg acgggtcgac gtgtgcatag cgggcgcttc
     4981 ggactcgccg atctcaccca tcacgatggc gtgcttcgac gccatcaagg cgacctcccc
     5041 gaacaacgac gaccccgagc acgcgtccaa gccgttcgac aagcaccgca acgggttcgt
     5101 catgggcgag ggcggagccg tcctgatcct ggaggagctc gaacacgcgc gggcccgtgg
     5161 cgcacgtgtg tactgcgaga tcggcggcta tgccaccttc ggcaacgcct accacatgac
     5221 cggcctcacc cgtgagggtc tggagatggc gcgtgcgatc gacggcgccc tcgcgcacgc
     5281 ccggttggac ggctccgaca tcgactacgt caacgcccac ggctccggca cccagcagaa
     5341 cgaccggcac gagacagccg ccgtgaaacg gtccctgggc gaccacgcgt accggacgcc
     5401 gatgagttcg atcaagtcga tggtgggcca ctccctgggc gccatcggtg cgatcgaggt
     5461 cctggcctgc gtcctggctc tcgtccatca ggtcgtgccg cccacggcga actacacgac
     5521 cccggacccc gagtgcgacc tcgactacgt gccgcgcacc gcacggcagg tcaaactccg
     5581 caacgtgttg tccgtcggca gtggcttcgg cggcttccag tccgcggtgg tgctgacccg
     5641 cccagatggg aggacctgat gaagccgcag caaaaacggc gtgccgccat caccggcata
     5701 ggcgtcgtcg cccccaacgg actgagcacc gagaccttct ggaagtccac cgagaagggc
     5761 ctctgtgtac tggaccgggt gacccgcgag gggtgcgacc atctgcccct gcggatcgcc
     5821 ggcgaggtcc gcggattcga cccggtggcg ctgatcgagg agcggttcct cgtccagacc
     5881 gaccggttca cccacttcgc gatggccgcc gccgacctcg ccctggagga cgcccggctc
     5941 ggccgcacct acaccggcga cgagccgttc gggatcggcg tggtcaccgc cgcggggtcc
     6001 ggcggcggcg agttcgggca gcgcgaactg cagcggctgt ggggacaggg gcccagattc
     6061 gtgggcccgt accagtccat cgcctggttc tacgcggcca gcaccggcca gatctccatc
     6121 aggggcggct tcaagggacc gtgcggcgtc gtggccagcg acgaggccgg cggtctggac
     6181 gccctggcgc acgccgcccg agccgtccgg cgcggcaccg acgtggtcgt ggccggtgcc
     6241 gccgaggctc cgctggctcc gtattcgatg gtctgccagc tcggctacga ggacctcagc
     6301 accagcgacg acccggcctg cgcctaccgg cccttcaccg gcgctgcctg cggattcgtg
     6361 cccgccgagg ggggcgccat gctcgtcgtc gaggaggaga cggtcgcacg gggccgggag
     6421 gccgcgatcc gggccttcgt cgccgggcac ggtgcgacgt tcaccggcgc gtcctgctgg
     6481 gcgcagtccc gggagggtct ggcgcacgcg atacgcgacg cgctgaacga ggcccggtgc
     6541 gcgcccgagg agatcgacgt cgtgttcgcg gacgcgttcg gcgtaccgga ggccgaccgt
     6601 gccgaggcgc tggcgatcgc cgacgtcctg ggcgcacagg gccggcgcgt gccggtgacg
     6661 gcacccaaga ccggcatcgg ccgggcgtac tgcggagcgc cggtgctgga cgtggcggcg
     6721 gcggtgctcg ccatggaaca cggtctcgta ccgcccaccc cgaacgtctt cgacccctgc
     6781 tacgacctcg acctggtgac cgggagcgct cgccccgccg agctgcgcac ggcgctggtc
     6841 ctcagccggg gactcatggg ctcgaacgca gcgctggtgc tgcggcacgg cgctcatgcc
     6901 ctctcatgag aaggagaaag agatggacaa ccaactgacc gtccaggagc tggccgctct
     6961 gatgaagaag gcggccggcg tcacggtgga cgtgaaggag ctggaggacc ggctcgactc
     7021 cggcttcgcc gagttcgggc tcgactcgct cggcctgctg ggcatcgtcg gggagctgga
     7081 gaacggccac ggcgcggtgc ttccccccga cgcggagcgc tgccggaacc cgcgcgagtt
     7141 cctcgacctc gtcaacaaca acatcgtcgc tggagcctga tgtgtctgga cacaccgaga
     7201 acgacatcat catcgccgcc cccctggacc tcgtctggga catgaccaac gacatcgaga
     7261 gctggccgca gctgttcagc gagtacgcgt ccgtcgatgt cctgagccgg gagaacggca
     7321 cgaccacctt ccgcctcacc atgcacccgg acgagaacgg caaggtctgg agctgggtct
     7381 cggaacggac gcccgaccgg gagaagctga cggtcacggc ccgccgcgtc gagaccggcc
     7441 ccttcgagta catgaacatc aggtgggtgt actcgcagac ccccgacggc accaggatgc
     7501 actggacgca ggacttcgcg atgaagcccg aagctcccgt cgacgacgcc tggatgaccg
     7561 acaacatcaa ccgcaactcg gtcgtccaga tggaactcat ccgggacaag gtcgaacagc
     7621 acgcccgcga gcgcaggacc gcttccgtca ccaccgactg atccccgacg ccggaccggc
     7681 cgcggaccgc ccccttcggg cggtccgccc ggcttggcgc cgacgaaagg aagttccccc
     7741 atgcaccaag ccctgatcgt ggcccggatg gcgccgggtt cggccccgga catcgcgaag
     7801 gtgttcgcgg agtccgacag cggcgaactg ccgcacctcg tgggcgtcac ccgccgcacc
     7861 ctgttccagt tcaacgacgt gtacatgcac ctggtggagg cggaccggcc gcccggcccc
     7921 gcgatcgccg aggtggccgg ccacccggcc ttccgggaca tcagcgacag gctctccacc
     7981 tacgtgagcg cgtacgaccc gacgacgtgg cgcagcccga aggacgcgat ggcccaggag
     8041 ttctaccgct gggaacgcga gagctgaggg cctgataggc ctgacacgtg tcaggtcggg
     8101 tcaggcaggt acggtgcatt cgaacgcgtg caggtacgcg ttgaccggcc ggatctcacc
     8161 gacggtcaga ccggcctccg tcatgcgggt gaccaggctt tccttggtgt gcttggctcc
     8221 tccgacgttg aggagcagca tcaggtccat ggacgtggtg aacttcatcg acggggtgtc
     8281 gtcgacgagg ttctcgatga tgacgaccct ggctccgggg cgggccttcg tcatcacgtt
     8341 ccgcagggtt ctgcgggtgc tgtcgtcgtc ccattccagg atgttcttga tgatgtacag
     8401 gtcggcctcg accgggatct cctcgcggca gtccgcgccg acgatccggg cccgcgcggc
     8461 gagcgggccg ccgtcacgca gccgggggtc cgccttctcc accacgcggg gcaggtcgag
     8521 cagggttccc cggatgtccg ggtgcttctc cagcaggctg gcgagcacat gtccctggcc
     8581 gccgccgatg tcggcgaccg tcgacacgcc ggtcaggtcg aggagctccg cgacgtcccg
     8641 cgccgactgc tcgctggacg tggtcatggc ctggttgaag acctgggcgg agtcgtgcgc
     8701 gtcctcgtgc aggtagtcga agaacccctt gccgtacagc tcgtggaaga cgctgccgcc
     8761 cgaccgcacc gcctcgtcga gccgcggcca ggcgtcccag gtccagggtt cggtgcacca
     8821 gagggagatg taccgaaggc tgtgcggatc gtcttcgcgc agcagccggg acatgctggt
     8881 gtggacgaac cgtccgtcgt cgttctgcgc gaagatgccg tagcaggaca gggcccggag
     8941 cagcctcagc aggggccgcg gctcggtgcc cagcacggcc gcgagttctt ccgcggtggc
     9001 gggcttctcg ccgagcgcgt cggcgacgcc gagcttggcc gccgcccgga cggccgcggc
     9061 gcacgccgcc ccgaagacga gctccctcag ctgcatggcc ggggccggct ggggggcggg
     9121 actcactgtg gtcatttacc gtctcgattc gcttggtcga gcagacaggc gggccggccg
     9181 gtcagcacat gcccgcgggt tcggagcgct gacacgagtt gtgctcgaac ttgttgccgg
     9241 tgccggtgtc ccggttggcc aggtcggccg tcgaattacc cgacagcacg ttgtcccgga
     9301 tgacattgcg cttgttcggc gtgccgacga agctcttgta cagcaccact ccgccggaga
     9361 acacggactt gcccacgttg tctcggatgt cgttgtactc cacgaccgtg tcctcggtac
     9421 cggtgagaac aattccggac ccctggatgt agggcagccg agccgtcttc gcgcagtatt
     9481 tgttgttctc gtggacgcca ttgccccgaa tggccagggc tccggcgtgc ggcttgccct
     9541 cgtcgcctac ggcgaacagc ccgctgcagt tcgcgctgag ttcgttctcc tcgacggtga
     9601 cattccgcag acgcctgacg gtgagtccga tccggttgcc ctccagcctg ttgtggcgga
     9661 cgacggtgcc ccgcgtgtcc gtggcgccgg cctcggtgtc caccgtgttg gagaggaaca
     9721 cgcccgactc ggcgtttccc cgggcgacgt tttcctcgag cacgctccgg acggaccgct
     9781 cgatggcgat gccccagttc ttgttcttgt ctgcggccac gtcccgcacg gtcagcttgt
     9841 cggtgttgga cgcccacagg ccgttcttct tgtagccggt cagcgtgagc gagcggacgc
     9901 tggtacgcgt gacggggtgg gcggacgtgc cctcgacgca gatcccgttg ccggcggccg
     9961 cgcacttgtc ggcggccctg gtcgccgccg gcatcagcac ggtccgctcc cccgccccgc
    10021 ggatggtcag accggacttc gtgacgagga cgctctgatg gtaggtgccg ggcgagatga
    10081 caatggtgtc gcccgactgc gcggcatcga ccgctttctg gatcgattcc cccggatgca
    10141 cgacatgagc agtcgcggca ttggccgccg ggatacagct cagtcccgac gccaccacga
    10201 ttgcggcgca accgaggtat gcgaaatctt ttttgttcat gggtcgaagt tatggtccat
    10261 caatccgacc cgccatgtgt ggtcacccgt ttactgcacg tcgggcgcga atggcccaat
    10321 ccggagcctg aaagggcgtc atgacccctc tgttcaggag ggccggggcc ataaatccgg
    10381 cttcgtctat ttcgagttcg gccggggtgg ccgaggccgg aatggtgaag cggccggagt
    10441 ggtgaggcgg ccggagggtt ccagccccgg cgccggtgcg cggggtcagc cgcgggtggc
    10501 ccggcgcagg gtgtggcgga ccgcgcgctg ggcgaccggg cccaggtcct ccaacgccgt
    10561 cagcaggagt gccaattggt ccagtgccgc gagggcggcc ccggcccctt ccgcgtccac
    10621 cccctcgtac agctcgatcc ccacgaacga cgccgccacc gcccgcgcca gccccgccgg
    10681 gtcggcgaac tcgcccagcg gggtcggggt caggacgcgg gtcagcacct tctcgatctc
    10741 cgcgatccag aggtcgaggc cggccgccgt cgcgggtgcg aggcgtggct gcgtctgggc
    10801 gccggcgagc agctggccga gcatcgccac atggccctcg gccttctcct gctcgtgcag
    10861 ctcgcggccc agcgcgagga gctcggccag ggtggcgacc tcgtccagcc gtgcccggta
    10921 gcgcgccacc acctgctctg cgccgtgccg gcaggccgcg gccagcagtt cgtccaccga
    10981 tccgaagtgg tagaagacga gcgcctggtt gactccggcg gccgcggcga tcgcacgggc
    11041 ggacgtcttg gcgatcccct gctcggagag cgcccgcagc gctccttcga ggagcttcgt
    11101 cttcgtcgtc tcgctcac
//
