LOCUS       BGC0000272              8927 bp    DNA     linear   BCT 26-OCT-2000
DEFINITION  Streptomyces collinus putative spore pigment polyketide synthase
            gene cluster, complete sequence.
ACCESSION   BGC0000272
VERSION     BGC0000272
KEYWORDS    .
SOURCE      Streptomyces collinus
  ORGANISM  Streptomyces collinus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:18:03
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /db_xref="taxon:42684"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces collinus"
                     /strain="DSM 2012"
                     /type_material="type strain of Streptomyces collinus"
     protocluster    1..8927
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[4211:6730]"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
                     /tool="antismash"
     proto_core      4212..6730
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
     cand_cluster    1..8927
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(t2ks and t2clf)"
                     /kind="single"
                     /product="T2PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..8927
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000272"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8927
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T2PKS"
                     /region_number="1"
                     /rules="(t2ks and t2clf)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             615..2270
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050:monooxygenase FAD-binding (Score: 331; E-value:
                     2.3e-100)"
                     /gene_functions="biosynthetic-additional (t2pks) OXY
                     (Score: 151.5; E-value: 8.9e-47)"
                     /gene_kind="biosynthetic-additional"
                     /note="PKSA-ORF7"
                     /product="PKSA putative hydroxylase"
                     /protein_id="AAG26878.1"
                     /transl_table=11
                     /translation="MNERGGQTGPHTISVPVLIVGGSLVGLSTSLFLGRLGVPXMLVER
                     HSGTSIHPRGRGNNVRTMELFRVAGVEKAIQDAAATLAVNKGILQTPTLTGDAGEWLFR
                     EIDPGGGLARFSPSAWCLCSQNDLEPVLLDHARGHGGDLRFSTELMSFKAGATGVTAVV
                     KSRETGEHTTIRADYLVAADGPRSPVREQLGIGQSGPGDLFHNVSLTFRSRRLADVVGE
                     RHFIVCYLTNPEADGALLPVDNRENWVFHAPWHPEHGQTLEEFTDEWCVEHIRRAVGVA
                     DLDVEITGKAPWHAAQRVARSYRSGRVFLAGDSAHEMSPTGASVPTPVSRTPTTSPGNW
                     PRCSTAGRGSACWTPTTPSAVPWPRPPARVPRPGRPSTAIPDSPRRPEGAAAPSAASST
                     WPSGTTTRRRAVVGADPAAPVVPESLDLTGGPGSRAPHLWVRHGDERMSTLDLYERSLV
                     LLSDAAEPNGWHEAALRLADDPALPLRSYRVGTGPQADLVPEDAADWAERHGTGPGGAV
                     LVRPDGFVAWRSPGPDPAAEDTLRQVLRTGLAVG"
     gene            2604..4036
                     /gene="PKSA-ORF8"
                     /pseudo=""
     CDS             4212..5480
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (4-391). E-value:
                     3.4e-50. Score: 163.0. Matches aSDomain:
                     nrpspksdomains_AAG26879.1_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2ks"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 448.2; E-value:
                     4.9e-136)"
                     /gene_functions="biosynthetic-additional (t2pks) KS (Score:
                     700.6; E-value: 1.4e-213)"
                     /gene_kind="biosynthetic"
                     /note="type-II polyketide synthase alpha subunit;
                     alpba-subunit; PKSA-ORF1"
                     /product="PKSA beta-ketoacylsynthase subunit alpha"
                     /protein_id="AAG26879.1"
                     /sec_met_domain="t2ks (E-value: 6.7e-204, bitscore: 666.4,
                     seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRRVAVTGIGIVAPGGIGVPAFWNLLAAGRTATRAITFFDPSGL
                     RSRIAAECDFDPTAHGLDPRDVERSDRYIQFAMVAGDEAVRDAGLDLAAEDPWRVGVSL
                     GTAVGGTTRLEHDYVLVSHGGQRWDVDHREAGPDLHRAFTPGALASTVAERFGARGPVQ
                     TVSTGCTSGLDAVGYAFHTVEEGRADICIAGASDSPISPITMACFDAIKATSPNNDDPA
                     HASRPFDADRDGFVMGEGGAVLVLEELEHARGPRCARVLRTRRLRHFGNAYHMTGLTSE
                     GVEMARAIDDALDHARLDPTAIDYVNAHGSGTKQNDRHETAAVKRVLGPHAYETPMSSI
                     KSMVGHSLGAIGAIELVACVLALTHQVVPPTANYETPDPECDLDYVPREARERKLDSVL
                     SVGSGFGGFQSAVVMNRPRETSR"
     aSDomain        4224..5384
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAG26879.1_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="3.40E-50"
                     /label="AAG26879.1_PKS_KS.1"
                     /locus_tag="AAG26879.1"
                     /protein_end="391"
                     /protein_start="4"
                     /score="163.0"
                     /tool="antismash"
                     /translation="VAVTGIGIVAPGGIGVPAFWNLLAAGRTATRAITFFDPSGLRSRI
                     AAECDFDPTAHGLDPRDVERSDRYIQFAMVAGDEAVRDAGLDLAAEDPWRVGVSLGTAV
                     GGTTRLEHDYVLVSHGGQRWDVDHREAGPDLHRAFTPGALASTVAERFGARGPVQTVST
                     GCTSGLDAVGYAFHTVEEGRADICIAGASDSPISPITMACFDAIKATSPNNDDPAHASR
                     PFDADRDGFVMGEGGAVLVLEELEHARGPRCARVLRTRRLRHFGNAYHMTGLTSEGVEM
                     ARAIDDALDHARLDPTAIDYVNAHGSGTKQNDRHETAAVKRVLGPHAYETPMSSIKSMV
                     GHSLGAIGAIELVACVLALTHQVVPPTANYETPDPECDLDYVPREAR"
     CDS_motif       4893..4931
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AAG26879.1_0001"
                     /evalue="5.00E-03"
                     /label="PKSI-KS_m4"
                     /locus_tag="AAG26879.1"
                     /protein_end="240"
                     /protein_start="227"
                     /score="10.2"
                     /tool="antismash"
                     /translation="FDADRDGFVMGEG"
     CDS             5477..6730
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (8-409). E-value:
                     2.1e-26. Score: 84.6. Matches aSDomain:
                     nrpspksdomains_AAG26880.1_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2clf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093:Beta-ketoacyl synthase (Score: 582.6; E-value:
                     5.6e-177)"
                     /gene_functions="biosynthetic-additional (t2pks) CLF
                     (Score: 548.6; E-value: 1.9e-167)"
                     /gene_kind="biosynthetic"
                     /note="type-II polyketide synthase beta subunit; chain
                     length factor; PKSA-ORF2"
                     /product="PKSA beta-ketoacylsynthase subunit beta"
                     /protein_id="AAG26880.1"
                     /sec_met_domain="t2clf (E-value: 8.3e-142, bitscore: 461.4,
                     seeds: 26, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEPRPQRVAVTGIGVVAPNGSSTETFWKSTREGISVLDRVTREG
                     CEHLPLRVAGQVRGFDPSAAIEERFLVQTDRFTHFAMAAADLALDDARLPRSVTDEVPF
                     SVGVVTAAGSGGGEFGQRELQQLWGKGSRFVGPYQSIAWFYAASTGQISIRRGFKGPCS
                     VVAADEAGGLDALAHAARAVRRGTDVIVAGATEAPIAPYSVVCQLGYEELSTVDDPTRA
                     YRPFTSAACGFVPAEGGAMVVVEAEGTARERGAPVRAHLAGHAATFTGASRWEESREGL
                     AQAVRGALAEAGCAPEEIDVVFADALGVPEADRAEALAIADALGAHGTRVPVTAPKTGI
                     GRGYCAAPLMDAAAAVLSMEHGLIPPTPNVFDICHDLDLVTGRARTAEVRTALVLSRGL
                     MGSNSALVLRHGAADASS"
     aSDomain        5501..6703
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAG26880.1_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="2.10E-26"
                     /label="AAG26880.1_PKS_KS.1"
                     /locus_tag="AAG26880.1"
                     /protein_end="409"
                     /protein_start="8"
                     /score="84.6"
                     /tool="antismash"
                     /translation="VAVTGIGVVAPNGSSTETFWKSTREGISVLDRVTREGCEHLPLRV
                     AGQVRGFDPSAAIEERFLVQTDRFTHFAMAAADLALDDARLPRSVTDEVPFSVGVVTAA
                     GSGGGEFGQRELQQLWGKGSRFVGPYQSIAWFYAASTGQISIRRGFKGPCSVVAADEAG
                     GLDALAHAARAVRRGTDVIVAGATEAPIAPYSVVCQLGYEELSTVDDPTRAYRPFTSAA
                     CGFVPAEGGAMVVVEAEGTARERGAPVRAHLAGHAATFTGASRWEESREGLAQAVRGAL
                     AEAGCAPEEIDVVFADALGVPEADRAEALAIADALGAHGTRVPVTAPKTGIGRGYCAAP
                     LMDAAAAVLSMEHGLIPPTPNVFDICHDLDLVTGRARTAEVRTALVLSRGLMGSNSALV
                     LR"
     CDS             6763..7017
                     /NRPS_PKS="Domain: PP-binding (9-76). E-value: 1e-10.
                     Score: 33.9. Matches aSDomain:
                     nrpspksdomains_AAG26884.1_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1129:acyl carrier protein (Score: 79.6; E-value:
                     2.3e-24)"
                     /gene_functions="biosynthetic-additional (t2pks) ACP
                     (Score: 71.3; E-value: 1.1e-22)"
                     /gene_kind="biosynthetic-additional"
                     /note="ACP; PKSA-ORF3"
                     /product="type-II polyketide synthase acyl carrier protein"
                     /protein_id="AAG26884.1"
                     /sec_met_domain="PP-binding (E-value: 1.3e-11, bitscore:
                     33.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDRISVEELSELMKKCTGVTVAPEELRRDGTAFESLGVDSLGLL
                     GIVGELENRYGTPMPTDAERSKTPRQFLDLVNGALLAGA"
     aSDomain        6790..6990
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAG26884.1_PP-binding.1"
                     /evalue="1.00E-10"
                     /label="AAG26884.1_PP-binding.1"
                     /locus_tag="AAG26884.1"
                     /protein_end="76"
                     /protein_start="9"
                     /score="33.9"
                     /tool="antismash"
                     /translation="LSELMKKCTGVTVAPEELRRDGTAFESLGVDSLGLLGIVGELENR
                     YGTPMPTDAERSKTPRQFLDLV"
     CDS             7019..7498
                     /NRPS_PKS="Domain: Polyketide_cyc2 (3-145). E-value:
                     3.3e-18. Score: 58.3. Matches aSDomain:
                     nrpspksdomains_AAG26881.1_Polyketide_cyc2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1128:cyclase/dehydrase (Score: 221.8; E-value:
                     2.1e-67)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC C9-C14
                     (Score: 243.2; E-value: 1.8e-75)"
                     /gene_kind="biosynthetic-additional"
                     /note="PKSA-ORF4"
                     /product="PKSA putative cyclase I"
                     /protein_id="AAG26881.1"
                     /transl_table=11
                     /translation="MTGHTQNEITIAAPVDLVWEVTNDLENWPQLFSEYASVEILSREG
                     HRTTFRLTMHPDENGTVWSWVSEREPDRDALTVKARRVETGPFAHMDIHWRYEEVPAGT
                     RMVWTQDFAMKPDAPVDDAWMTDNINRNSKVQMALIRERVEKIAAERGASAGLTD"
     aSDomain        7028..7453
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAG26881.1_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="3.30E-18"
                     /label="AAG26881.1_Polyketide_cyc2.1"
                     /locus_tag="AAG26881.1"
                     /protein_end="145"
                     /protein_start="3"
                     /score="58.3"
                     /tool="antismash"
                     /translation="HTQNEITIAAPVDLVWEVTNDLENWPQLFSEYASVEILSREGHRT
                     TFRLTMHPDENGTVWSWVSEREPDRDALTVKARRVETGPFAHMDIHWRYEEVPAGTRMV
                     WTQDFAMKPDAPVDDAWMTDNINRNSKVQMALIRERVE"
     CDS             7519..7851
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1225:Polyketide synthesis cyclase (Score: 162.2;
                     E-value: 5.3e-50)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC
                     C4-C21/C2-C23|C2-C19 (Score: 162.5; E-value: 6.1e-51)"
                     /gene_kind="biosynthetic-additional"
                     /note="PKSA-ORF5"
                     /product="PKSA putative cyclase II"
                     /protein_id="AAG26882.1"
                     /transl_table=11
                     /translation="MHQALIVARMAPGSAPDIAKVFEESDRGELPHLVGVTRRSLFQFG
                     DVYLHLIEAERDPGPAIAKITGHPEFRDVSERLSAYVSAYDPATWRSPKDAMAQRFYLW
                     EREPAG"
     CDS             complement(7964..8926)
                     /codon_start=1
                     /note="PKSA-ORF6"
                     /product="PKSA putative O-methyltransferase"
                     /protein_id="AAG26883.1"
                     /transl_table=11
                     /translation="MRRRPARGRPSGNRRRPRRGPHGRGGHRGRGEDRTRTAATAPASP
                     VLPRRLHGTPGRDVRAHRRLPAAARGRPPQPARHHAVVHRAVDLGRLAEAGRGGAHRPQ
                     RGGGPVRQGVLPLPQRGRPGVGGRLQPRHDDLQRAVGAGRRRVPRPHGMHLGGRRRRRP
                     GARRGEPARQVPGPARDXARPPPGGGERRPETAHGRRPRGPRARRARRRPDGRPGEGRR
                     LRDQEHPGVGRREHHPHAAQRPRGRRPRDARGGHREXRRRLALDAVQHRDGPAAAPQRR
                     RGQAHPAEHGGPAHRGRPGHRRHQPGQPLSARVRLHIPG"
ORIGIN
        1 atgctcctcg atcccggctg gcaggccctg ttcgcgctga cctcccggct gatcgcgctg
       61 cgccaccgcc acccggtgct caggcgccgg gcgttcttct cgggccgggc ccagaccgcg
      121 gacggnctgc gcgacctggc ctggttcacg tcccggggca cggagatgac ggagcaggac
      181 tggtacgcgc ccgccgccac tctgggcatg tacctgtccg gccgggacat cccggggcgc
      241 gacgagcgcg gggagcagat cctcgacgac agcttcctgg ccntcctgca cgcgggcgaa
      301 gggccggtgg acttcaccct gccggggccg ccgtgggccg agcggtacga ggtcgtcgtc
      361 gacacgagcc gggaggagca gggggaggcg ccgggcgtgg agcaccgggc ggggacggag
      421 atcacgatgc cggcgcgggc cgtgctgctg ctgcgggtgg cggggtgacc ccggcggggc
      481 ggccggcggg tgcggacggg cagcacgcag caggcgggtg aagcctgagc cactgtcacc
      541 ttcccggggc atgcatgagg atctccgccg catccggccg ggtcggcggt atcgatgcgc
      601 tggaggcgac agtgatgaac gaacgaggcg ggcagacggg tccgcacacc atcagcgtcc
      661 cggtcctcat cgtcggcggc tccctggtcg gcctgtcgac ctcgctgttc ctgggacggc
      721 tgggcgtgcc gnncatgctg gtggagcgcc actcaggcac ctccatccac ccgcgaggcc
      781 gtggcaacaa cgtccgcacg atggagctgt tccgggtggc gggcgtcgag aaggcgatcc
      841 aggacgcggc cgccaccctg gccgtcaaca agggcatcct ccagacgccc acgctgaccg
      901 gtgacgcggg cgagtggctg ttcagggaga tcgacccggg gggcggcctg gcccgcttca
      961 gccccagcgc ctggtgcctg tgcagccaga acgacctcga accggtcctg ctcgaccacg
     1021 cccgcggaca cggcggggac ctgcgcttct ccaccgagct gatgtcgttc aaggccggcg
     1081 ccaccggtgt gaccgccgtg gtcaagagcc gcgagacggg tgagcacacc accatccgcg
     1141 cggactatct cgtcgccgcg gacggcccgc gcagccccgt gcgtgagcag ctcggcatcg
     1201 gtcagagcgg tcccggcgac ctgttccaca acgtcagcct caccttccgc tcccgccgcc
     1261 tcgccgacgt cgtgggtgag cgccacttca tcgtctgcta cctgacgaac ccggaggccg
     1321 acggcgccct gctgccggtg gacaaccgcg agaactgggt cttccacgcc ccctggcacc
     1381 ccgaacacgg ccagaccctg gaggaattca cggacgagtg gtgcgtcgag cacatccgcc
     1441 gggcggtcgg ggtcgccgac ctcgatgtgg agatcaccgg caaggcgccg tggcacgccg
     1501 ctcagcgggt ggcccgcagc taccggtccg gacgggtgtt cctggccggt gactcggcgc
     1561 acgagatgtc tcccaccggg gcttcggttc caacaccggt atccaggacg cccacaacct
     1621 cgcctggaaa ctggccgcgg tgctcgacgg ctgggcgggg gagcgcctgc tggacaccta
     1681 cgacgccgag cgccgtcccg tggccgaggc caccagcgcg cgtgccgcgg cccggtcggc
     1741 cgagcacagc catcccggat tcgccgcgcc ggccggaggg ggcggcggcc cccagcgcgg
     1801 catcctcaac gtggccctcg ggtaccacta cccgcaggcg cgccgtcgtc ggcgccgatc
     1861 ccgcggcccc cgtggtcccc gagagcctcg acctgaccgg cggccccggc agcagggccc
     1921 cgcacctgtg ggtncgtcac ggggacgaac gcatgtcgac gctcgacctc tacgagcggt
     1981 ccctggtgct gctcagtgac gccgccgagc cgaacggatg gcacgaggcc gcgctccgcc
     2041 tcgccgacga tccggccctg ccgctcaggt cgtaccgggt gggcaccggg cctcaggccg
     2101 acctggtacc ggaggacgcc gccgactggg cggagcgcca cggcacgggg cccgggggcg
     2161 ccgtgctcgt ccggcccgac gggttcgtcg catggcggtc gccgggtccg gacccggccg
     2221 ccgaggacac gctgcgccag gtcctgagga ccgggctggc cgtcggctga atcccgggct
     2281 ccgaagcggc gagtcccccg acgggtgctg aggggcgggc ggccacgccc gcatcacccg
     2341 gacgggggac cgcgaccggc aggccgcgga ccacgcggac ggcccggttg tcctgacccg
     2401 tcccgtccgg cggccactcg acccggtcac ccgcggacgc cgtccgagtc gcgcgatgtg
     2461 cggggacggc cacaggatcg aggaacgccg acgtcgcatc cctgcggccg gcgttcacgt
     2521 tccccgccgc gaaggagatg tgcgcaggat gaacaccacg tccgaacggg gggcaggttc
     2581 gcgggcgcga caggcctcgc accatgtctc ccagtccgtg ttcgacggtt cccggcgtcc
     2641 gcgtcgtcct tctggtcgac gtccacgacg gggcccagca ggagttcctc gaggcttacg
     2701 aacagttgtg caaccaggtc gcgtccgtcc ccgggcatgt cagcgaccag ctgtgccagt
     2761 cgatcgagaa tccctcccag tggctcatca cgagtgagtg ggagagcgcc ccgccgttcc
     2821 tcgcctgggt caacagcgag gagcacgtgc ggatggtgga gccgctgcac agctgcgtgc
     2881 gcgacacgcg gtcgctgcgc ttccacgtcg tccgcgagac cggcggaccg gcgaagggcg
     2941 ccgaccaggc ggagcgccgc ctccagacgg cgccccgaat cggtgacggt gtgatccggc
     3001 acgcgctcac gttcaccgtg aagccgggca gcgaggagat cgtggccaag atcctcgccg
     3061 actacgactc gcccgagccg aaggtcgacg acagcacccg gctgtgccgc acctccctgt
     3121 tcatgcacgg caaccgggtg gtgcgggccg tcgaggtgcg gggcgacctg ctggccgcgc
     3181 tgcgccacgt cgcccggcag cccgaggtgc gggccgtcga ggaggcgatc aatccccacc
     3241 tcgaacagga ccgggacctg ggcgaccccg aatccgcccg ggtcttcttc accagggcgc
     3301 gctgccggcc gtccaccatg tgacggcggg cggcaggagc cgcagggcga gcggtacgcc
     3361 ctgtactacc ggcgcggccg gagcgcggca tgcagctcgc cgaactcctg gcccatcggg
     3421 acgagacggc cgcggacgac ccgcgcagcc cggtcctgcg cagcacgatc ttccagcgcg
     3481 acgacgtcgt cgtacggctg atcgacgtgc acggctcgat cgaggaggcc ggccccggcc
     3541 acatcctcgg tctgtccgac tcggcgcagg tgaccgagct gacgacgctc ctggacgacg
     3601 tcgcggacat ggacgtggcc atgtcgaagg acggcagcct cgcccgcctg ctcgaactgg
     3661 cgcgcatgga tctcgtcacc gaccgccggt cgcccgacgc ctgattcgcc atccggcggg
     3721 aggggcgacg ccgcctctcc cgcggtggcg cagcacctca acggaggaac gcgtgatcac
     3781 atctcgtccc aacatcgtga gcctgagcga cgtcgagccc aaccgccgcc gaggcggtga
     3841 cctgcgcgcc atgctcactc cggccgcggt gggcgccacc agtggattca tgggcgtggc
     3901 gctcatcgaa cccggtgacc gaatcggtga gcactaccac ccgtactccg aggagttcgt
     3961 gtacgtggtg tgcgggcagc tcgaggtgga cctggacggc gagccgcacc cgctccggcc
     4021 cgagcagggc tgctgatccc cgtcaacgtg cgccaccgct tccgcaatgt cggcaaggag
     4081 gaggccaggc tggtcttcca cctcggcccc ctggcgccga gtccgccgct gggccacgtc
     4141 gacaccgagg agaccgacgc cgagtccgcg gtggtgggag cggaggctgc cgcacgacgt
     4201 caggtccact catgaccagg cgagtggcgg tcaccggcat aggcatcgtc gctcccgggg
     4261 gcataggcgt accggcgttc tggaacctcc tggcggccgg gcgcaccgcg acacgggcca
     4321 tcacgttctt cgacccgtcc ggtctgcggt cgcggatcgc cgccgagtgc gacttcgacc
     4381 ccactgccca cggcctggac ccgcgggacg tggaacgaag cgaccgctac atccagttcg
     4441 ccatggtcgc gggcgacgag gcggtacggg acgccggtct cgatctcgcc gccgaggacc
     4501 cctggcgcgt cggcgtctcg ctcggcaccg cggtcggcgg caccacccgg ctggagcacg
     4561 actacgtgct ggtcagccac ggcgggcagc gctgggacgt ggaccaccgg gaagcgggcc
     4621 ccgacctgca ccgggcgttc acgcccggcg ccctcgcctc gacggtggcg gagcggttcg
     4681 gagcgcgcgg cccggtgcag accgtgtcca cgggctgcac ctccgggctc gacgcggtcg
     4741 gctacgcctt ccacaccgtg gaggaaggcc gggccgacat ctgcatagcc ggcgcctcgg
     4801 actcgccgat ctccccgatc accatggcct gcttcgacgc gatcaaggcc acctccccga
     4861 acaacgacga cccggcccac gcctcgcgtc ccttcgacgc cgaccgcgac ggattcgtca
     4921 tgggcgaggg cggcgccgta ctcgtcctgg aggagctcga acacgccagg ggcccgcggt
     4981 gcgcgcgtgt actgcgaact cggcggctac gccacttcgg caacgcctac cacatgaccg
     5041 gcctcaccag cgagggcgtg gagatggccc gcgccatcga cgacgccctc gaccacgcgc
     5101 ggctcgaccc caccgcgatc gactacgtca acgcgcacgg ctcgggcacc aagcagaacg
     5161 accgccacga gaccgcagcg gtcaagcggg tcctgggacc gcacgcctac gaaaccccca
     5221 tgagttccat caagtcgatg gtgggccact ccctgggcgc catcggagcg atcgaactcg
     5281 tcgcctgtgt gctggccctg acgcaccagg tggtgccgcc gaccgcgaac tacgagaccc
     5341 ccgaccccga gtgcgacctg gactacgtcc cccgcgaggc gagggagcgc aagctcgaca
     5401 gcgtgctctc ggtgggcagc ggattcggcg gcttccagtc cgcggtggtc atgaaccggc
     5461 cgagggagac gtcacgatga gcgaacctcg tccacagcgc gtggccgtca cgggtatcgg
     5521 tgtggtcgcg cccaacggat ccagtaccga gaccttctgg aagtccacca gggagggcat
     5581 cagcgtcctg gaccgcgtca cccgggaggg ctgtgagcat ctgccgctgc gggtggcggg
     5641 ccaggtgcgc ggcttcgacc cgtcggcggc gatcgaggag cgcttcctgg tccagaccga
     5701 ccggttcacg cacttcgcga tggcggcggc cgacctcgcg ctcgacgacg cccggctccc
     5761 ccggtccgtg accgacgagg tgccgttctc cgtcggtgtg gtcaccgcgg ccggttccgg
     5821 cggcggcgag ttcggacagc gcgagctcca gcagctgtgg ggcaagggca gtcgcttcgt
     5881 cggtccgtac cagtccatcg cctggttcta tgccgccagc accggccaga tctccatccg
     5941 ccgcggtttc aagggcccct gctcggtcgt cgccgccgac gaggccggcg gcctggacgc
     6001 cctcgcccac gcggcacgag ccgtacggcg cggcaccgat gtgatcgtgg ccggggcgac
     6061 cgaggcgccg atcgcgccct actcggtggt ctgccagctc ggctacgagg aactcagcac
     6121 ggtcgacgac ccgacccgtg cctaccgccc gttcacgtcc gcggcctgcg ggttcgtgcc
     6181 cgctgagggg ggcgccatgg tcgtggtgga ggcggagggc acagcccggg agcggggcgc
     6241 gcccgtgcgg gcccacctgg ccggacacgc ggcgaccttc acgggggcct cccgctggga
     6301 ggagtcccgg gaaggactcg cccaggccgt ccggggtgct ctggcggagg ccggctgcgc
     6361 ccccgaggag atcgacgtgg tgttcgccga cgccctcggc gtaccggagg cggaccgggc
     6421 ggaggccctg gccatcgccg acgctctcgg ggcccatggc acccgggtgc ccgtcaccgc
     6481 gccgaagacc ggcatcggcc ggggctactg cgcggcgccg ctgatggacg ccgcggcggc
     6541 ggtgctgtcg atggagcacg gtctgatccc gcccaccccc aacgtcttcg acatctgcca
     6601 cgacctcgac ctcgtgacag gccgggcccg taccgccgag gtacgcacgg cgctggtgct
     6661 gagccgggga ctcatggggt cgaactcggc gctggtgctg aggcacggcg ccgccgacgc
     6721 ctcgtcgtga ggcgccgtgg aaccggcaag gaagggaatg gcatgaacga ccgcatctcg
     6781 gtggaagagc tgtccgaact catgaagaag tgcaccggtg tcaccgtcgc cccggaggaa
     6841 ctccgccgcg acgggacggc cttcgaaagc ctcggcgtcg actcgctggg cctgctgggc
     6901 atcgtgggcg agctggagaa ccggtacggc acgccgatgc cgaccgacgc cgaacgctcc
     6961 aagacgcccc ggcagttcct cgacctcgtc aacggtgccc tcctggcagg agcgtgacat
     7021 gaccggacac acacagaacg agatcaccat cgccgcgccg gtggacctgg tctgggaggt
     7081 gaccaacgac ctggagaact ggccgcagct gttcagcgag tacgcctccg tcgagatcct
     7141 ctcccgggag ggccacagga cgaccttccg gctgacgatg cacccggacg agaacggcac
     7201 cgtgtggagc tgggtctccg agcgcgagcc ggaccgcgac gcgctcaccg tcaaggcccg
     7261 ccgcgtcgag acgggaccct tcgcccacat ggacatccac tggcggtacg aggaggtccc
     7321 ggccggcacg cggatggtct ggacgcagga cttcgcgatg aagccggacg cgccggtcga
     7381 cgacgcgtgg atgacggaca acatcaaccg gaactccaag gtgcagatgg ccctgatccg
     7441 tgagcgcgtc gagaagatcg ccgcggagcg cggtgcgagt gccggtctga ccgactgagc
     7501 cggacggaga acagtcccat gcaccaagcc ctgatcgtcg cccgcatggc cccggggtcg
     7561 gcgcccgaca tcgccaaggt gttcgaggag tccgaccggg gggagctgcc gcacctcgtc
     7621 ggtgtgaccc ggcgcagcct cttccagttc ggcgatgtgt acctgcacct gatcgaggcc
     7681 gagcgcgacc ccgggccggc catcgccaag atcaccggcc atcccgagtt ccgggacgtc
     7741 agcgagcgcc tgtccgcgta cgtcagcgcg tacgaccccg cgacctggcg ttccccgaag
     7801 gacgccatgg cgcagcgctt ctacctctgg gagcgcgagc cggccggctg accgccgccg
     7861 gcacgagacg gccgccgggt ccgcagcgtc gcggacccgg cggccgttgt gcatctgcac
     7921 cggggagtcc cgggatcacc gggtcccccg ggaagcggtc ggatcagccg gggatgtgca
     7981 gtcgaacgcg tgcagatagg ggttgaccgg gctgatgtcg tcgatgacca ggccggcctc
     8041 ggtgagccgg tccaccatgc tctgccgggt gtgcttggcc ccgccgacgt tgaggagcag
     8101 cagcaggtcc atcgcggtgc tgaaccgcat cgagggcgag tcgtcgacga ngttctcgat
     8161 gaccaccacg cgcgtcccgg ggccgccggc ctcgcggacg ttgcgcagcg tgcgggtggt
     8221 gctctcgtcg tcccactcca ggatgttctt gatcacgtag acgtcggcct tcaccgggac
     8281 ggccgtccgg acgtcgccgg gcacgacgtg cgcgcggtcc gcgaggtcgc cgcccgtgcg
     8341 cagtctcggg acggcgttct ccaccacccg ggggaggtcg agcancgtcc cgcgcaggtc
     8401 cgggtacttg tcgagcaggc tcgccacgac gtgcccctgg ccgccgccga cgtcggccac
     8461 cgaggtgcat cccgtgaggt cgaggaacgc ggcgacgtcc tgcgccgact gcacgctgga
     8521 ggtcgtcatg gcgcggttga agacgtccgc cgactccggg gcgtcctcgt tgaggtaggg
     8581 gaagaactcc ttgccgtaca ggtcctccac cacgttgcgg ccggtgcgca ccgcctcgtc
     8641 cagcttcggc cagacgtccc aggtccacgg ctcggtgcac cacagcgtga tggcgcgcag
     8701 gctgtggggg tcgtcctcgc gcagcagccg ggagacgtcg gtgtgcgcga acgtcccgtc
     8761 cgggcgttcc gtgaagacgc cgtggcagga cagggctcgc aggagccgtc gcagcggtcc
     8821 gggttcggtc ttcaccgcgg ccgcgatgtc ctccgcggcc atggggtcct cgccgaggac
     8881 gtcggcgatt cccagacggg cggccgcgcg cagggcggcg gcgcatg
//
