LOCUS       BGC0000275             13123 bp    DNA     linear   BCT 26-MAY-1995
DEFINITION  Streptomyces glaucescens tcm operon.
ACCESSION   BGC0000275
VERSION     BGC0000275
KEYWORDS    .
SOURCE      Streptomyces glaucescens
  ORGANISM  Streptomyces glaucescens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     Original source text: Streptomyces glaucescens DNA; Streptomyces
            glaucescens DNA; and Streptomyces glaucescens DNA.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:18:20
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13123
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..13123
                     /db_xref="taxon:1907"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces glaucescens"
     protocluster    1..13123
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[6778:9285]"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_malonyl_elongations="8|9 (Score: 657.9; E-value:
                     1.1e-200)"
                     /t2pks_molecular_weights="acetyl-CoA_8 (Da): 376.444"
                     /t2pks_molecular_weights="acetyl-CoA_9 (Da): 418.481"
                     /t2pks_product_classes="tetracenomycin"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
                     /tool="antismash"
     proto_core      6779..9285
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_malonyl_elongations="8|9 (Score: 657.9; E-value:
                     1.1e-200)"
                     /t2pks_molecular_weights="acetyl-CoA_8 (Da): 376.444"
                     /t2pks_molecular_weights="acetyl-CoA_9 (Da): 418.481"
                     /t2pks_product_classes="tetracenomycin"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
     cand_cluster    1..13123
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(t2ks and t2clf)"
                     /kind="single"
                     /product="T2PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..13123
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000275"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..13123
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T2PKS"
                     /region_number="1"
                     /rules="(t2ks and t2clf)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(59..742)
                     /gene="tcmR"
     CDS             complement(59..>742)
                     /codon_start=1
                     /function="repressor of tetracenomycin C resistance and
                     export gene"
                     /gene="tcmR"
                     /gene_functions="regulatory (smcogs) SMCOG1215:TetR family
                     transcriptional regulator (Score: 155.2; E-value: 3.7e-47)"
                     /gene_kind="regulatory"
                     /product="tetracenomycin C resistance and export gene
                     repressor"
                     /protein_id="AAA67508.1"
                     /transl_table=11
                     /translation="MVDSAETDTPSTRSTPNGPGLRQRKLRRTRDQLIREALELFLAQG
                     YEHTTVEQIAEAVEVHPRTFFRHFASKEEVALTPISAIDEAFLAALEVRPAGENPLQAM
                     SGAFRAVLGRVRDGELEGVDGALHMAMMRLVERTPGLLAEYLRRSEEMEGRLARIIAAR
                     EGVDLDDDFRPRFIVAVFKAVGRVVSREWYLRADTDLEALSVAFESALDSLRPELFADW
                     RRPGA"
     regulatory      complement(745)
                     /gene="tcmR"
                     /note="p1 promoter of tcmR repressor gene, apparently
                     inducible"
                     /regulatory_class="promoter"
     regulatory      820
                     /gene="tcmA"
                     /regulatory_class="promoter"
     regulatory      complement(848)
                     /gene="tcmR"
                     /note="p2 promoter of tcmR repressor gene, apparently
                     constitutive"
                     /regulatory_class="promoter"
     CDS             878..2494
                     /codon_start=1
                     /gene="tcmA"
                     /gene_functions="transport (smcogs) SMCOG1005:Drug
                     resistance transporter, EmrB/QacA (Score: 395.9; E-value:
                     3.1e-120)"
                     /gene_kind="transport"
                     /product="tetracenomycin C resistance and export protein"
                     /protein_id="AAA67509.1"
                     /transl_table=11
                     /translation="MSTETHDEPSGVAHTPASGLRGRPWPTLLAVAVGVMMVALDSTIV
                     AIANPAIQQDLHASLADVQWITNGYLLALAVSLITAGKLGDRFGHRQTFLVGVAGFAVT
                     SAAIGLSGSVAAIVVFRVLQGLFGALMQPSALGLLRVTFPPGKLNMAIGIWSGVVGAST
                     AAGPIIGGLLVQHVGWEAVFFINVPVGLAALVAGLVILTDARAERAPKSFDVSGIVLLS
                     GAMFCLVWGLIKAPAWGWGDLRTLGFLAAAVLAFAGFTLRESRATEPLMPLAMFRSVPL
                     SAGTVLMVLMAFSFIGGLFFVTFYLQNVHGMSPVESGVHLLPLTGMMIVGAPVSGIVIS
                     RFGPGGPLVVGMLLTAASLWGMSTLEADSGMGITSLWFVLLGLGLAPVMVGTTDVIVSN
                     APAELAGVAGGLQQSAMQVGGSLGTAVLGVLMASRVGDVFPDKWAEANLPRVGPREAAA
                     IEDAAEVGAVPPAGTLPGRHAGTLSEVVHSSFISGMGLAFTVAGAVALVAAAVALFTRK
                     AEPDERAPEEFPVPASTAGRG"
     gene            878..2557
                     /gene="tcmA"
     regulatory      2522..2557
                     /gene="tcmA"
                     /note="putative transcription terminator; putative"
                     /regulatory_class="terminator"
     gene            complement(2609..3424)
                     /gene="tcmP"
     CDS             complement(2609..>3424)
                     /codon_start=1
                     /function="putative O-methyltransferase for conversion of
                     tetracenomycin E to tetracenomycin A2"
                     /gene="tcmP"
                     /gene_functions="biosynthetic-additional (t2pks) MET
                     carboxy_O (Score: 277.5; E-value: 1.9e-85)"
                     /gene_kind="biosynthetic-additional"
                     /product="O-methyltransferase"
                     /protein_id="AAA67510.1"
                     /transl_table=11
                     /translation="MMGETIRLTGVHETLLATLQARALDNRQPRPVLGDATAEELLRRI
                     DYDFSRIRTATKDMRIVTFRARKLDEWAGRFLAVHPDAVVLHLACGLDSRAFRMDVPDT
                     VEWIDVDVPDVIELRSRLYPERDGYRMIGASVTTEDWLDTVPRNRPALVVAEGLTPYLR
                     EADGEEMLRRVIRHLPTGAVMFDAVLPWTLRFAKYSQLLRATGATFGWGIGNPRSLESR
                     VPGLRFQEQWSMLDSPFLADARPVEKWVGAGMRSIPQLRFAHRLLRYTF"
     misc_feature    complement(2747..2749)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3954..5639
                     /gene="tcmG"
     CDS             3954..5639
                     /codon_start=1
                     /function="putative hydroxylase for conversion of
                     tetracenomycin A2 to tetracenomycin C"
                     /gene="tcmG"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050:monooxygenase FAD-binding (Score: 414.9; E-value:
                     9e-126)"
                     /gene_functions="biosynthetic-additional (t2pks) OXY
                     (Score: 312.0; E-value: 1.8e-95)"
                     /gene_kind="biosynthetic-additional"
                     /product="hydroxylase"
                     /protein_id="AAA67511.1"
                     /transl_table=11
                     /translation="MSTEEVPVLIVGGGLTGLSAALFLSQHGVSCRLVEKHRGTTVLTR
                     ASGISSRTMELLRGVGLERTVIERGPKLVEGARWRELGQPADQIPWVVIRANGLHDLEN
                     AVTVEEPSLDVGHLSPTRPYWCGQDRLEPILRDEAVRRGARIDFNTRMEAFTADESGVT
                     ATIVDQATGEQSTVRARYLIAADGVRSPVRETLGITRTGHGTIGNAMSVLFKADLRDTV
                     KGRRFVICYLPNPDEPGVLQLPEVPAVLQLFDEDRWIFGFFFDPRETSPEQFTDERCAQ
                     IIRTATGLPGLPVEVQMARPWEMSHNSARSYRSGRVFLAGDAAHVHPPAGAFGANGGIQ
                     DAHNLAWKLAAVLKGTAGDALLDTYEQERLPIGAAVADQAWIRHTWRLNDSEELRRALR
                     ESTLVATGYRYTSDAVLGAAYPEPIPAAHDLTGRPGYRVPHVWLGRGGERVSTVDLGGD
                     AFVVLAGPDGGEWQAAADKVAQDLGVPVHCHPVGGDGQLTDPDGAFLGTTGLTRNGALL
                     IRPDGFVAWRAEYLPEDAAGELRSALERILARTSGTPGGTALEG"
     protein_bind    3987..4052
                     /bound_moiety="FAD"
                     /gene="tcmG"
                     /note="binding site in TcmG protein; putative"
     gene            5644..5982
                     /gene="tcmH"
     CDS             5644..5982
                     /codon_start=1
                     /function="required for conversion of tetracenomycin F2 to
                     tetracenomycin D3; putative hydroxylase"
                     /gene="tcmH"
                     /product="hydroxylase"
                     /protein_id="AAA67512.1"
                     /transl_table=11
                     /translation="MATISPSPDLFTLVNVFGVAPEKQRELRDHLVQVTEDLIRHMPGF
                     VSATFHLSRDGEQVVNYAQWRSEADFRAMHADPRLQPHFDYCRSVSRPKPIFCEVTHSF
                     GATSPEGA"
     gene            5985..6314
                     /gene="tcmI"
     CDS             5985..6314
                     /codon_start=1
                     /function="required for conversion of tetracenomycin F2 to
                     tetracenomycin D3"
                     /gene="tcmI"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1225:Polyketide synthesis cyclase (Score: 129.6;
                     E-value: 7.4e-40)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC
                     C4-C21/C2-C23|C2-C19 (Score: 161.0; E-value: 1.7e-50)"
                     /gene_kind="biosynthetic-additional"
                     /protein_id="AAA67513.1"
                     /transl_table=11
                     /translation="MAYRALMVLRMDPADAEHVAAAFAEHDTTELPLEIGVRRRVLFRF
                     HDLYMHLIEADDDIMERLYQARSHPLFQEVNERVGQYLTPYAQDWEELKDSKAEVFYSW
                     TAPDS"
     gene            6328..6777
                     /gene="tcmJ"
     CDS             6328..6777
                     /codon_start=1
                     /function="possibly required for conversion of
                     tetracenomycin F2 to tetracenomycin D3"
                     /gene="tcmJ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MannoseP_isomer"
                     /gene_functions="biosynthetic-additional (t2pks) CYC C5-C18
                     (Score: 203.5; E-value: 2.2e-63)"
                     /gene_kind="biosynthetic-additional"
                     /protein_id="AAA67514.1"
                     /sec_met_domain="MannoseP_isomer (E-value: 1.1e-09,
                     bitscore: 28.0, seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAQHEGEQTVAAAAKIAMSSVAPSHRQGGSTRALLTPSSVGAT
                     SGFLGHIELAPGESVTEHYHPFSDKYLYLIEGSLVVRVNGEEVRLERDEALFVTRGQRH
                     RIENRGNVPARVVFQISPLAPRPELGHVDTEPVPNPAAAPPKVGG"
     gene            6779..8059
                     /gene="tcmK"
     CDS             6779..8059
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (8-393). E-value:
                     4.9e-57. Score: 185.6. Matches aSDomain:
                     nrpspksdomains_tcmK_PKS_KS.1"
                     /codon_start=1
                     /gene="tcmK"
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2ks"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 484; E-value:
                     6.6e-147)"
                     /gene_functions="biosynthetic-additional (t2pks) KS (Score:
                     759.2; E-value: 2.4e-231)"
                     /gene_kind="biosynthetic"
                     /note="putative; putative"
                     /product="tetracenomycin polyketide synthase condensing
                     enzyme"
                     /protein_id="AAA67515.1"
                     /sec_met_domain="t2ks (E-value: 1.2e-234, bitscore: 768.5,
                     seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRHAEKRVVITGIGVRAPGGAGTAAFWDLLTAGRTATRTISLFD
                     AAPYRSRIAGEIDFDPIGEGLSPRQASTYDRATQLAVVCAREALKDSGLDPAAVNPERI
                     GVSIGTAVGCTTGLDREYARVSEGGSRWLVDHTLAVEQLFDYFVPTSICREVAWEAGAE
                     GPVTVVSTGCTSGLDAVGYGTELIRDGRADVVVCGATDAPISPITVACFDAIKATSANN
                     DDPAHASRPFDRNRDGFVLGEGSAVFVLEELSAARRRGAHAYAEVRGFATRSNAFHMTG
                     LKPDGREMAEAITAALDQARRTGDDLHYINAHGSGTRQNDRHETAAFKRSLGQRAYDVP
                     VSSIKSMIGHSLGAIGSLELAACALAIEHGVIPPTANYEEPDPECDLDYVPNVAREQRV
                     DTVLSVGSGFGGFQSAAVLARPKETRS"
     aSDomain        6803..7957
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_tcmK_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="4.90E-57"
                     /label="tcmK_PKS_KS.1"
                     /locus_tag="tcmK"
                     /protein_end="393"
                     /protein_start="8"
                     /score="185.6"
                     /tool="antismash"
                     /translation="VVITGIGVRAPGGAGTAAFWDLLTAGRTATRTISLFDAAPYRSRI
                     AGEIDFDPIGEGLSPRQASTYDRATQLAVVCAREALKDSGLDPAAVNPERIGVSIGTAV
                     GCTTGLDREYARVSEGGSRWLVDHTLAVEQLFDYFVPTSICREVAWEAGAEGPVTVVST
                     GCTSGLDAVGYGTELIRDGRADVVVCGATDAPISPITVACFDAIKATSANNDDPAHASR
                     PFDRNRDGFVLGEGSAVFVLEELSAARRRGAHAYAEVRGFATRSNAFHMTGLKPDGREM
                     AEAITAALDQARRTGDDLHYINAHGSGTRQNDRHETAAFKRSLGQRAYDVPVSSIKSMI
                     GHSLGAIGSLELAACALAIEHGVIPPTANYEEPDPECDLDYVPNV"
     gene            8056..9285
                     /gene="tcmL"
     CDS             8056..9285
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (9-406). E-value:
                     2.1e-32. Score: 104.4. Matches aSDomain:
                     nrpspksdomains_tcmL_PKS_KS.1"
                     /codon_start=1
                     /function="required for tetracenomycin polyketide synthase;
                     function unknown"
                     /gene="tcmL"
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2clf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093:Beta-ketoacyl synthase (Score: 635.7; E-value:
                     4.4e-193)"
                     /gene_functions="biosynthetic-additional (t2pks) CLF 8|9
                     (Score: 657.9; E-value: 1.1e-200)"
                     /gene_kind="biosynthetic"
                     /protein_id="AAA67516.1"
                     /sec_met_domain="t2clf (E-value: 1.1e-193, bitscore: 633.1,
                     seeds: 26, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAPAPVVVTGLGIVAPNGTGTEEYWAATLAGKSGIDVIQRFDPH
                     GYPVRVGGEVLAFDAAAHLPGRLLPQTDRMTQHALVAAEWALADAGLEPEKQDEYGLGV
                     LTAAGAGGFEFGQREMQKLWGTGPERVSAYQSFAWFYAVNTGQISIRHGMRGHSSVFVT
                     EQAGGLDAAAHAARLLRKGTLNTALTGGCEASLCPWGLVAQIPSGFLSEATDPHDAYLP
                     FDARAAGYVPGEGGAMLVAERADSARERDAATVYGRIAGHASTFDARPGTGRPTGPARA
                     IRLALEEARVAPEDVDVVYADAAGVPALDRAEAEALAEVFGPGAVPVTAPKTMTGRLYA
                     GGAALDVATALLSIRDCVVPPTVGTGAPAPGLGIDLVLHQPRELRVDTALVVARGMGGF
                     NSALVVRRHG"
     aSDomain        8083..9273
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_tcmL_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="2.10E-32"
                     /label="tcmL_PKS_KS.1"
                     /locus_tag="tcmL"
                     /protein_end="406"
                     /protein_start="9"
                     /score="104.4"
                     /tool="antismash"
                     /translation="TGLGIVAPNGTGTEEYWAATLAGKSGIDVIQRFDPHGYPVRVGGE
                     VLAFDAAAHLPGRLLPQTDRMTQHALVAAEWALADAGLEPEKQDEYGLGVLTAAGAGGF
                     EFGQREMQKLWGTGPERVSAYQSFAWFYAVNTGQISIRHGMRGHSSVFVTEQAGGLDAA
                     AHAARLLRKGTLNTALTGGCEASLCPWGLVAQIPSGFLSEATDPHDAYLPFDARAAGYV
                     PGEGGAMLVAERADSARERDAATVYGRIAGHASTFDARPGTGRPTGPARAIRLALEEAR
                     VAPEDVDVVYADAAGVPALDRAEAEALAEVFGPGAVPVTAPKTMTGRLYAGGAALDVAT
                     ALLSIRDCVVPPTVGTGAPAPGLGIDLVLHQPRELRVDTALVVARGMGGFNSALVVR"
     CDS_motif       8125..8247
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_tcmL_0001"
                     /evalue="7.80E-01"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="tcmL"
                     /protein_end="64"
                     /protein_start="23"
                     /score="1.6"
                     /tool="antismash"
                     /translation="EYWAATLAGKSGIDVIQRFDPHGYPVRVGGEVLAFDAAAHL"
     CDS_motif       8722..8760
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_tcmL_0002"
                     /evalue="3.50E-04"
                     /label="PKSI-KS_m4"
                     /locus_tag="tcmL"
                     /protein_end="235"
                     /protein_start="222"
                     /score="13.6"
                     /tool="antismash"
                     /translation="FDARAAGYVPGEG"
     gene            9358..9609
                     /gene="tcmM"
     CDS             9358..9609
                     /NRPS_PKS="Domain: PP-binding (7-66). E-value: 9.8e-12.
                     Score: 37.2. Matches aSDomain:
                     nrpspksdomains_tcmM_PP-binding.1"
                     /codon_start=1
                     /function="acyl carrier protein for tetracenomycin
                     polyketide synthase"
                     /gene="tcmM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1129:acyl carrier protein (Score: 90.8; E-value:
                     7.2e-28)"
                     /gene_functions="biosynthetic-additional (t2pks) ACP
                     (Score: 89.8; E-value: 2e-28)"
                     /gene_kind="biosynthetic-additional"
                     /product="acyl carrier protein"
                     /protein_id="AAA67517.1"
                     /sec_met_domain="PP-binding (E-value: 2.1e-12, bitscore:
                     37.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQIGLPRLVEIIRECAGDPDERDLDGDILDVTYQDLGYDSIALL
                     EISAKLEQDLGVSIPGEELKTPRHTLHLVNTETAGEVA"
     aSDomain        9379..9555
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_tcmM_PP-binding.1"
                     /evalue="9.80E-12"
                     /label="tcmM_PP-binding.1"
                     /locus_tag="tcmM"
                     /protein_end="66"
                     /protein_start="7"
                     /score="37.2"
                     /tool="antismash"
                     /translation="RLVEIIRECAGDPDERDLDGDILDVTYQDLGYDSIALLEISAKLE
                     QDLGVSIPGEELKT"
     CDS             9611..11095
                     /NRPS_PKS="Domain: Polyketide_cyc (10-140). E-value:
                     7.6e-17. Score: 53.6. Matches aSDomain:
                     nrpspksdomains_tcmN_Polyketide_cyc.1"
                     /codon_start=1
                     /function="O-methyltransferase"
                     /function="putative polyketide cyclase"
                     /gene="tcmN"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042:O-methyltransferase (Score: 290.4; E-value:
                     3.1e-88)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC C9-C14
                     (Score: 240.4; E-value: 1.4e-74)"
                     /gene_functions="biosynthetic-additional (t2pks) MET C11O
                     (Score: 546.0; E-value: 6.7e-167)"
                     /gene_kind="biosynthetic-additional"
                     /note="possible internal translational start site at
                     position 9950; SAM binding site at position 10617 to 10636"
                     /protein_id="AAA67518.1"
                     /transl_table=11
                     /translation="MAARTDNSIVVNAPFELVWDVTNDIEAWPELFSEYAEAEILRQDG
                     DGFDFRLKTRPDANGRVWEWVSHRVPDKGSRTVRAHRVETGPFAYMNLHWTYRAVAGGT
                     EMRWVQEFDMKPGAPFDNAHMTAHLNTTTRANMERIKKIIEDRHREGQRTPASVLPTEL
                     HAQQLLLLAASGRLARIVHVLTELRIADLLADGPRHVAELAKETDTHELSLYRVLRSAA
                     SVGVFAEGPVRTFSATPLSDGLRTGNPDGVLPLVKYNNMELTRRPYDEIMHSVRTGEPA
                     FRRVFGSSFFEHLEANPEAGEFFERFMAHWSRRLVLDGLADQGMERFSRIADLGGGDGW
                     FLAQILRRHPHATGLLMDLPRVAASAGPVLEEAKVADRVTVLPGDFFTDPVPTGYDAYL
                     FKGVLHNWSDERAVTVLRRVREAIGDDDARLLIFDQVMAPENEWDHAKLLDIDMLVLFG
                     GRERVLAEWRQLLLEADFDIVNTPSHTWTTLECRPV"
     gene            9611..11149
                     /gene="tcmN"
     aSDomain        9641..10030
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_tcmN_Polyketide_cyc.1"
                     /domain_subtypes="Polyketide_cyc"
                     /evalue="7.60E-17"
                     /label="tcmN_Polyketide_cyc.1"
                     /locus_tag="tcmN"
                     /protein_end="140"
                     /protein_start="10"
                     /score="53.6"
                     /tool="antismash"
                     /translation="VNAPFELVWDVTNDIEAWPELFSEYAEAEILRQDGDGFDFRLKTR
                     PDANGRVWEWVSHRVPDKGSRTVRAHRVETGPFAYMNLHWTYRAVAGGTEMRWVQEFDM
                     KPGAPFDNAHMTAHLNTTTRANMERI"
     regulatory      11121..11149
                     /gene="tcmN"
                     /note="potential; putative"
                     /regulatory_class="terminator"
     gene            11192..12211
                     /gene="tcmO"
     CDS             11192..12211
                     /codon_start=1
                     /function="putative O-methyltransferase for conversion of
                     tetracenomycin B3 to tetracenomycin E"
                     /gene="tcmO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042:O-methyltransferase (Score: 272; E-value:
                     1.2e-82)"
                     /gene_functions="biosynthetic-additional (t2pks) MET
                     C13O|C17O (Score: 433.2; E-value: 1.3e-132)"
                     /gene_kind="biosynthetic-additional"
                     /product="O-methyltransferase"
                     /protein_id="AAA67519.1"
                     /transl_table=11
                     /translation="MTPHTHVRGPGDILQLTMAFYGSRALISAVELDLFTLLAGKPLPL
                     GELCERAGIHPRGARDFLDALVALGLLEREGEDTYRNSPAADRHLDRRKPGYVGGYARL
                     ADTKLFPVWARLTEALRTGEKQVPSQGGFFGGYADPEAARGFLGAMDAVNGGVGHSLAG
                     ALDWTEYSSFVDLGGARGNLAAHLHRAHPHLRATCFDLPEMEPFFQEHMKSLETTDQVR
                     FAGGDFFTDPLPRADVFIVGHILHYFGLRQREALIARIHQALTPGGAVLVYDRMIDDDR
                     RSAALSLLGSLNMLLTSDEGREYTPAECVRWLSDAGFTDVRTTAVSGPDTLAIGRKPR"
ORIGIN
        1 gcggccgccc cctcgggggc ctggcgcccc gtcagccacc cgggggtctg acgctccgtc
       61 aggcccccgg gcgccgccag tcggcgaaca actccggccg cagcgagtcc agagcggact
      121 cgaacgccac ggagagcgcc tccagatccg tgtcggcgcg caggtaccac tcccggctga
      181 cgacgcggcc gaccgccttg aacaccgcca cgatgaaccg cggacggaag tcgtcgtcca
      241 ggtcgacgcc ctcacgcgcg gcgatgatcc gggcgagtcg gccctccatc tcctcgctgc
      301 gccgcaggta ctccgcgagg agcccggggg tgcgctcgac cagccgcatc atcgccatgt
      361 gcaacgcgcc gtccacgccc tccagttcac cgtcgcgcac gcgcccaagg acggcccgga
      421 aggcaccgct catggcctgc agcgggttct cgcccgccgg ccggacctcc agcgcggcga
      481 ggaacgcctc gtcgatggcg ctgatggggg tgagggccac ctcctccttg ctggcgaagt
      541 ggcggaagaa cgtgcgcgga tgcacctcga ccgcctcggc gatctgctcc acggtcgtgt
      601 gctcgtaacc ctgcgccagg aacagctcca gggcctcgcg gatcagctga tcacgcgtac
      661 ggcgcagctt ccgctggcgc aggccggggc cgttgggggt gctgcgggtg ctgggggtgt
      721 cggtttcggc ggagtccacc cgccaaccct atcttgcgcc acctggtgac acctgcatgc
      781 gattgtcaaa tgtcactgac tgctgttagc ttcggggcca tgttctcctc gtcacacacg
      841 cgtccgtcct gccggaaatc gttaggggga cgctgaaatg agcacggaaa cgcacgacga
      901 gccgtcaggc gtcgcacaca cccccgcttc cggtctgcgt ggacgtccct ggccgaccct
      961 gctcgcggtc gcggtcggcg tgatgatggt ggctctggac agcaccatcg tcgccatcgc
     1021 caacccggcc atccagcagg acctccacgc ctcgctggcc gatgtgcagt ggatcaccaa
     1081 cggctacctg ctggcgctcg cggtctccct gatcaccgcc ggcaaactgg gcgacaggtt
     1141 cgggcaccgg cagaccttcc tggtcggagt ggcgggcttc gccgtcacct ccgcggccat
     1201 cggcctgtcc ggcagcgtcg cggcgatcgt cgtcttccgc gttctccagg gcctgttcgg
     1261 cgctctgatg cagccgtcgg cgctgggcct gctgcgggtc acgttcccgc ccggcaagct
     1321 caacatggcg atcggcatct ggagcggcgt ggtcggcgcc tcgaccgcag ccggaccgat
     1381 catcggcggg ctcctggtgc agcacgtcgg ctgggaggcg gtcttcttca tcaatgtgcc
     1441 ggtcggcctc gcggcactcg tcgccggcct cgtcatcctg accgacgcgc gcgccgaacg
     1501 cgccccgaag tccttcgacg tctccggcat cgtgctcctg tccggggcca tgttctgcct
     1561 ggtgtggggt ctgatcaagg ctccggcctg gggctggggc gacctgcgga cgctgggctt
     1621 cctggccgcc gccgtgctcg ccttcgccgg attcacactg cgggagagca gggccaccga
     1681 gccgctgatg ccgctcgcca tgttccgttc cgtgccgctg tccgcgggca ccgtgctcat
     1741 ggtcctgatg gcgttctcgt tcatcggcgg actcttcttc gtcaccttct acctgcagaa
     1801 cgtccatggc atgagcccgg tcgagagcgg tgtgcacctg ctgccgctca ccggcatgat
     1861 gatcgtcggc gcccccgtgt ccggcatcgt gatcagccgg ttcggaccag gcgggccgct
     1921 ggtggtgggc atgctcctga cggcggcgtc cctgtggggg atgtcgacgc tggaagcgga
     1981 cagcggtatg gggatcacct cgctctggtt cgtgctgctg ggtctcggtc tcgcgccggt
     2041 gatggtgggg acgacggacg tcatcgtcag caacgccccg gccgaactgg ccggtgtggc
     2101 cggtggtctt cagcagtccg cgatgcaggt cggcggaagt ctcggcaccg ccgtactcgg
     2161 tgtcctcatg gcctcccggg tcggcgacgt cttccccgac aagtgggcgg aggcgaacct
     2221 gccgcgggtg ggtccgcggg aggccgcggc gatcgaggac gcggccgagg tgggagccgt
     2281 cccgccggcc ggcaccctcc ccggccggca cgccgggacg ctctccgagg tggtccactc
     2341 ctcgttcatc tcggggatgg ggctggcctt caccgtggcc ggtgccgtcg cgctcgtggc
     2401 cgccgccgtc gccctgttca ccaggaaggc cgagcccgac gagagggccc cggaggaatt
     2461 cccggtcccg gcgtccacgg cagggcgggg ctgaaagcct cggtccctgt ccgccggtga
     2521 atgccggcgg ggtccggtcc ggcggacccc gccggcaccg ctgtttccgc gatgcccgaa
     2581 cggaggggac ggaggaaaag tcccggaatc agaaggtgta acgcagcagc cggtgtgcga
     2641 aacgcagttg cggaatgctg cgcatgccgg ctcccaccca tttctcgacg gggcgggcgt
     2701 cggcgagaaa cggggaatcg agcatggacc actgctcctg gaaccgtaat cccggcaccc
     2761 tgctctccag ggagcgggga tttccgattc cccatccgaa ggtggcgccc gtggcgcgca
     2821 gaagttgtga atatttggcg aatcgaaggg tccacgggag tacggcgtcg aacatgaccg
     2881 cacccgtcgg aaggtgccgg atcacccgcc ggagcatttc ctctccgtct gcttcccgga
     2941 gatagggcgt cagcccttcg gcgaccacga gcgccggccg gttgcggggc accgtgtcca
     3001 gccagtcctc cgtggtgacc gacgctccga tcatccggta tccgtcgcgt tcgggataga
     3061 gccggctcct cagttcgatg acgtccggga cgtcgacgtc gatccattcc actgtgtcgg
     3121 gcacgtccat ccggaatgcc cggctgtcca gcccgcatgc gaggtgcagc accaccgcgt
     3181 ccggatggac cgcgaggaat cgaccggccc actcgtcgag cttcctggcg cggaacgtca
     3241 cgatccgcat gtccttggtg gctgttctga tgcggctgaa gtcgtagtcg atgcggcgca
     3301 gcagctcctc cgccgtcgcg tcccccagca cgggtcgcgg ttgtcgattg tccagggcac
     3361 gggcctgaag tgtggcgagc agcgtctcgt gtacgccggt cagccggatc gtttcaccca
     3421 tgggacagca gcttccgagg gggcaccgcg tgacgtcaag agaccccctc ggcgggccgt
     3481 ggcgaaaaga tctggacaca ttgccgtacg caaagtcggg acacttccag gtattcctcg
     3541 ccaaacagcg ggagcgttcg gccaggcgct tggctcgatc tgatcaacat gtcggaggga
     3601 ggcgccgtcg cggacggcct gatctttgtc gtcggaaagg taaggggatc gccaagaggt
     3661 aaaggattca tagggcatgt tcaaaagcat cggtcatcaa aaagggggtt cgtgtttaac
     3721 tccggagagt cgagggtata catgcccatc aattttccgg tccacttctg accgagtgtc
     3781 agtgacatct atccggtctc cccgccgaag attgttcgat gttgactcga cggaccggaa
     3841 gatgttcatc tggatgagcc aagtgccgct ctgctcgtcg gcccgagcaa atccccgatc
     3901 tcatcgcagg gatgactcgt gtgcccgttt ccgaccgacc gaaaggctgc atcttgtcca
     3961 ctgaagaagt tccggtactg atagtcggcg gaggtcttac cggactgtcc gcggcactgt
     4021 tcctcagtca gcacggagtt tcctgccgcc tggtggagaa gcaccgggga accacggtcc
     4081 tgacccgggc ctcggggatc agctcccgca cgatggaact gttgcggggc gtcggcctgg
     4141 aacggaccgt gatcgagcgc gggccgaaac tcgtcgaggg agcccgctgg cgggagctcg
     4201 gccagccggc ggaccagatc ccctgggtcg tcatccgggc caacggcctg cacgacctgg
     4261 agaacgcggt gaccgtcgag gaaccctcgc tcgacgtcgg ccacctcagc cccacccgcc
     4321 cctactggtg cgggcaggac cggctggaac ccatcctccg cgacgaggcc gtccgccgtg
     4381 gggcccgcat cgacttcaac acccgcatgg aggcgttcac ggcggacgag agcggggtca
     4441 ccgccacgat cgtcgaccag gccaccggcg agcagagcac ggtccgcgcc cggtacctga
     4501 tcgcggcgga cggggtgcgc agcccggtgc gggagacgct ggggatcacg cgcaccgggc
     4561 acggcacgat cggcaacgcc atgagcgtcc tgttcaaggc ggacctgcgg gacaccgtca
     4621 agggccggcg cttcgtcatc tgctacctgc cgaaccccga cgagcccggc gtcctccagc
     4681 tcccggaggt gccggccgtc ctccagctct tcgacgagga ccgctggatc ttcggcttct
     4741 tcttcgaccc ccgcgagacc tcgcccgagc agttcaccga cgagcgctgc gcgcagatca
     4801 tccggaccgc gaccgggctc cccggcctcc cggtcgaggt gcagatggcc aggccctggg
     4861 agatgtccca caacagtgcc cgctcctacc gcagcggccg ggtcttcctg gccggcgacg
     4921 ccgcccacgt gcacccaccg gccggtgcct tcggagcgaa cggcggtatc caggacgccc
     4981 acaacctggc ctggaagctg gcggccgtcc tgaagggaac ggccggcgac gccctgctcg
     5041 acacctacga gcaggaacgc ctgccgatcg gtgccgccgt cgccgaccag gcgtggatcc
     5101 ggcacacctg gaggctgaac gactccgagg agctgcgccg cgcgctgcgc gagtcgacgc
     5161 tggtcgccac cggttaccgg tacacctccg acgcggtcct cggtgccgcc tacccggagc
     5221 ccatccccgc ggcgcacgac ctcaccggcc ggccggggta ccgcgtgccc cacgtctggc
     5281 tgggccgggg cggggagcgc gtctcgacgg tcgacctcgg cggggacgcg ttcgtcgtgc
     5341 tcgccgggcc ggacggaggc gagtggcagg ccgcggccga caaggtcgcg caggacctgg
     5401 gcgtgccggt gcactgccac ccggtgggcg gcgacgggca actgaccgat cccgacggcg
     5461 cgttcctcgg caccacgggc ctcacccgga acggggcgct gctgatccgg ccggacggct
     5521 tcgtggcgtg gcgggccgag tacctgccgg aggacgccgc cggggagctg cggtccgcac
     5581 tggagcggat cctggcgcgg acctccggca cgcccggcgg cacggcgctg gagggctgac
     5641 cgcatggcga ccatctcccc ctcgccggac ctgttcaccc tcgtcaacgt cttcggcgtc
     5701 gcccccgaga agcagcggga actgcgcgac cacctggtgc aggtgaccga ggacctgatc
     5761 cggcacatgc cgggtttcgt ctccgcgacc ttccacctca gccgcgacgg cgaacaggtc
     5821 gtcaactacg cacagtggcg gtcggaggcc gacttccgcg ccatgcacgc cgacccgcgg
     5881 ctgcagcccc acttcgacta ctgccggtcc gtgtcccggc cgaagccgat cttctgcgag
     5941 gtcacccact ccttcggcgc cacgtcaccg gagggagcgt gaccatggcc taccgggcac
     6001 tgatggtcct gcgcatggac ccggcggacg cggagcacgt ggcagccgcc ttcgccgagc
     6061 acgacacgac cgaactgccg ctggagatcg gggtgcgacg gcgggtcctg ttccgcttcc
     6121 acgatctgta catgcacctc atcgaggccg acgacgacat catggagcgg ctctaccagg
     6181 cgcgcagcca cccgctgttc caggaggtca acgagcgcgt ggggcagtac ctcacgccgt
     6241 acgcgcagga ctgggaggag ctgaaggact ccaaggcgga ggtcttctac agctggaccg
     6301 cccccgactc ctgaccgccg gccatccgtg accaccgctc agcacgaagg agaacagacc
     6361 gtggcagccg ccgccaagat cgccatgtcc agtgtggcgc ccagtcaccg gcaggggggc
     6421 agcacccgcg ccctgctgac gccgtcgtcg gtgggtgcga cctccggatt cctcggccac
     6481 atcgaactgg cgcccgggga gtccgtcacc gagcactacc acccgttctc ggacaagtac
     6541 ctgtacctga tcgaagggag cctcgtggtc cgggtgaacg gcgaggaggt gcgcctggag
     6601 cgggacgagg ccctgttcgt gacccggggc cagcggcacc gcatcgagaa ccgaggaaac
     6661 gttcctgcac gggtggtctt ccagatctcg ccgctcgccc cgcggcccga actcggccat
     6721 gtcgacaccg agcccgtgcc gaacccggcg gcggcgccgc cgaaggtggg aggctgagat
     6781 gacccggcac gcggagaagc gggtggtgat caccggcatc ggtgtgcgag ccccgggagg
     6841 cgccggaacc gcggccttct gggacctgct caccgccgga cgcaccgcca ccaggacgat
     6901 ctccctgttc gacgccgcgc cctaccggtc ccggatagcc ggagagatcg acttcgaccc
     6961 gatcggcgag ggcctgtcgc cccggcaggc gtcgacgtac gaccgggcca cgcagctcgc
     7021 cgtcgtctgc gcccgcgagg cgctgaagga cagcgggctg gacccggccg cggtgaaccc
     7081 ggaacggatc ggggtgagca tcggcaccgc cgtcggctgc accaccggcc tcgaccgcga
     7141 gtacgcgcgg gtcagcgagg gcggctcgcg ctggctggtc gaccacacgc tcgcggtgga
     7201 gcagctcttc gactacttcg tcccgacctc catctgccgg gaggtcgcct gggaggcggg
     7261 ggccgaggga ccggtgacgg tggtgtcgac cgggtgcacc tcgggcctcg acgccgtcgg
     7321 atacggcacg gagctcatcc gggacgggcg cgccgacgtg gtcgtctgcg gcgccaccga
     7381 cgcgccgatc tcccccatca ccgtggcctg cttcgacgcc atcaaggcga cgtcggccaa
     7441 caacgacgac ccggcccacg cctcccgccc cttcgaccgc aaccgcgacg gcttcgtgct
     7501 cggggagggc tcggcggtct tcgtcctgga ggagctgtcc gccgcccgcc ggcgaggagc
     7561 gcacgcctac gcggaggtcc gcggcttcgc cacacggtcg aacgccttcc acatgaccgg
     7621 tctgaagccg gacggcaggg agatggcgga ggccatcacc gccgccctgg accaggcccg
     7681 ccgcaccggc gacgacctgc actacatcaa cgcgcacggc tccggcaccc ggcagaacga
     7741 ccggcacgag acggcggcct tcaagcgcag cctggggcaa cgggcgtacg acgtgcccgt
     7801 cagctccatc aagtccatga tcggccactc gctcggagcg atcggctccc tcgaactggc
     7861 cgcctgcgcg ctggcgatcg aacacggcgt gatcccgccc accgccaact acgaggaacc
     7921 cgaccccgaa tgcgacctcg actacgtgcc gaacgtcgcg cgggaacagc gggtcgacac
     7981 cgtgctcagc gtcggcagcg gattcggcgg cttccagagc gccgcggtgc tggcccggcc
     8041 gaaggagacc cgctcatgag cgcgccggcc cccgtcgtgg tcaccggcct gggcatcgtg
     8101 gcacccaacg gcaccggcac cgaggagtac tgggcggcca cgctcgccgg caagagcggc
     8161 atcgacgtca tccagcggtt cgacccccac ggctaccccg tccgggtggg aggcgaggtg
     8221 ctggccttcg acgccgccgc ccatctcccc gggcgcctgc tgccgcagac cgaccgcatg
     8281 acccagcacg ccctggtggc cgccgaatgg gccctggccg acgccgggct ggagcccgag
     8341 aagcaggacg agtacggcct cggcgtcctg acggccgccg gcgcgggagg gttcgagttc
     8401 ggccagcgcg agatgcagaa actctgggga acgggcccgg aacgcgtcag cgcctaccag
     8461 tcgttcgcct ggttctacgc ggtgaacacc ggccagatct ccatccgcca cggcatgcgc
     8521 gggcacagct ccgtgttcgt caccgagcag gccggcggtc tcgacgccgc cgcccacgcg
     8581 gcccgcctgc tgcgcaaggg gaccctgaac accgccctga ccggcggatg cgaagcctcc
     8641 ctctgcccct ggggcctggt cgcccagatc ccgagcggct tcctgagcga ggccaccgac
     8701 ccgcacgacg cctacctgcc cttcgacgcg cgggcggccg ggtacgtgcc cggcgagggc
     8761 ggagccatgc tggtcgccga gcgggcggac tccgcacgcg aacgggacgc cgccacggtc
     8821 tacgggagga tcgccgggca cgcctccacc ttcgacgccc gccccggcac cggccggccc
     8881 accgggccgg cccgggccat ccgcctggcc ctcgaagagg cccgggtcgc gcccgaggac
     8941 gtcgacgtgg tctacgccga cgccgctgga gtgccggccc tcgaccgcgc ggaggcggag
     9001 gccctcgccg aggtcttcgg gcccggggcc gtaccggtca ccgcgcccaa gacgatgacc
     9061 ggacggctct acgccggggg agcggccctc gacgtcgcga ccgcgctgct gtccatcagg
     9121 gactgtgtcg tcccgccgac cgtcggcacc ggtgcgcccg cgccggggct cggcatcgac
     9181 ctggtgctcc accagccccg tgagctgcgg gtggacaccg ccctggtcgt cgcgcggggc
     9241 atgggcggat tcaactccgc cctggtcgtc cggcgccacg gctgaccgag ccgccgcccc
     9301 cgctctccgc gcccgagcgc gcatcacctc atcacaccac tcatccggag gttcaccgtg
     9361 ccccagatcg gcctgccccg actcgtcgag atcatcaggg aatgcgcagg agaccccgac
     9421 gaacgggacc tggacggcga catcctcgac gtcacctacc aggacctcgg ctacgactcc
     9481 atcgccctgc tggagatctc cgcgaagctg gaacaggacc tcggggtgtc gataccgggg
     9541 gaggagctga agaccccccg gcacaccctc cacctcgtca acaccgagac ggccggggag
     9601 gtcgcctgac atggcagcgc gcacggacaa ctccatcgtc gtcaacgcgc cgttcgagct
     9661 cgtctgggac gtcaccaacg acatcgaggc atggccggag ctgttctccg agtacgccga
     9721 ggcggagatc ctccggcagg acggcgacgg cttcgacttc cggctcaaga cccgccccga
     9781 cgcgaacgga agggtctggg agtgggtctc ccaccgtgtg cccgacaagg gctcccgcac
     9841 ggtccgggcg caccgcgtcg agaccgggcc cttcgcctac atgaacctgc actggaccta
     9901 ccgggccgtg gccgggggca ccgagatgcg gtgggtgcag gagttcgaca tgaagcccgg
     9961 ggccccgttc gacaacgcgc acatgaccgc tcacctcaac acgacgacac gggcgaacat
    10021 ggagcgcatc aagaagatca tcgaggacag gcaccgggaa ggacagcgga ccccggcgtc
    10081 cgtcctgccc accgaactgc acgcccagca actgctgttg ctggccgcct ccgggcgcct
    10141 ggcgcggatc gtccacgtgc tgaccgagct gaggatcgcc gatctcctcg ccgacggccc
    10201 ccgtcacgtc gcggaactgg ccaaggagac ggacacccac gagctctccc tctaccgcgt
    10261 cctgcgcagc gccgcctccg tcggcgtctt cgcggagggg cccgtcagga ccttctccgc
    10321 gacgcccctg tcggacggac tgcgcaccgg caacccggac ggtgtgctgc ccctggtgaa
    10381 gtacaacaac atggagctga ccaggcggcc ctacgacgag atcatgcaca gtgtgcgcac
    10441 cggcgaaccg gccttccggc gggtgttcgg cagctcgttc ttcgagcacc tggaggccaa
    10501 cccggaagcc ggcgagttct tcgagcggtt catggcccac tggagccgcc ggctcgtcct
    10561 cgacggactc gccgaccagg gcatggagcg cttctcgcgc atcgcggacc tcggcggtgg
    10621 cgacggctgg ttcctcgccc agatcctccg gcgccacccg cacgccacgg gcctgctcat
    10681 ggacctcccg cgggtggccg cgtcggccgg ccccgtcctg gaggaggcca aagtcgccga
    10741 ccgggtcacc gtgctgcccg gcgacttctt cacggacccc gtccccaccg gctacgacgc
    10801 ctacctcttc aagggcgtgc tgcacaactg gtccgacgag cgggccgtga ccgtgctgcg
    10861 ccgggtccgg gaggccatcg gcgacgacga cgcacgcctg ctgatcttcg accaggtcat
    10921 ggcccccgag aacgagtggg accacgccaa gctgctggac atcgacatgc tggtgctctt
    10981 cggaggccgc gaacgggtgc tggccgagtg gcggcagctg ctgctcgagg cggacttcga
    11041 catcgtcaac acgccctccc acacctggac cacgctggag tgccgccccg tctagggcct
    11101 gtccggatcg tcccggcgcc gcggggtccg gcacccggcc ggaccccgcc cgaaccgcca
    11161 gcccccacgt tcaaggagag ccgagcacac catgacaccg cacacgcacg tccgcggacc
    11221 gggcgacatc ctccagctga ccatggcctt ctacggatcg cgcgccctca tcagcgcggt
    11281 cgaactggac ctgttcaccc tgctcgccgg gaaaccgctg ccgctcggcg agctgtgcga
    11341 gcgggcgggc atccatcctc gcggcgcgcg ggacttcctc gacgccctgg tggcactggg
    11401 actcctggag cgcgagggcg aggacaccta ccgcaacagc ccggccgccg accgccatct
    11461 ggaccggcgc aagcccgggt acgtcggcgg ctacgcccgg ctcgcggaca ccaagctgtt
    11521 ccccgtctgg gcacggctga cggaggccct gcgcacgggc gagaagcagg tgccgagcca
    11581 gggcggcttc ttcggcggct acgcggaccc ggaggcggcc cgcggcttcc tcggcgccat
    11641 ggacgccgtc aacggcggcg tcgggcacag cctggccggc gcgctcgact ggacggagta
    11701 ctcctccttc gtcgacctcg gcggcgcgcg cggcaacctc gccgcgcacc tgcaccgggc
    11761 tcacccgcac ctgcgggcca cctgtttcga cctgccggag atggaaccct tcttccagga
    11821 gcacatgaag tccctggaga cgacggacca ggtgcgcttc gccgggggcg acttcttcac
    11881 cgaccccctg ccgcgcgcgg acgtgttcat cgtcgggcac atcctccact acttcggtct
    11941 ccggcagcgc gaggcgctga tcgcccgcat ccaccaggcg ctcaccccgg gcggtgccgt
    12001 cctcgtctac gaccggatga tcgacgacga ccggcgcagt gccgcactga gcctgctcgg
    12061 cagcctcaac atgctgctga cctccgacga gggacgcgag tacacccccg cggagtgcgt
    12121 ccggtggctg tccgacgccg gattcaccga cgtccgcacc accgcggtga gcgggcccga
    12181 caccctcgcg atcggccgca aaccccggtg aggtggctcg gcaggaccgt ctggctgggg
    12241 ctgatcggcc tcggcgcgtt ctggaccggg cacctgcccc ccggcccgta cgggcaggac
    12301 cgggccgggt ccgccccgcc ggccggacac ccggaacggc tcgtccccac ggcgctcacc
    12361 gcccaggagc tgatcctgtc gcgcgagctg ggctggaccc gctgacctgc cgggaacccc
    12421 ggcccggtgc gcggcgggca ccccggaccg gggcacgtcc gcgtccgcgg gagtgctcgg
    12481 cggacgtacc ggcccgcccg gcctccccgg cggcgacgcg gcgccgtcgg gtgcgtggcc
    12541 gagccgcgcg ccccgtggcc gcggccggcg ccgcggggac ctctcacggg atcccgtgcc
    12601 tggccgcgcc ccggtcgggg aggcgaggac caccagcgcg cggccgggcc gcgcggtcag
    12661 gtgaaggtga ccgggccgcg cggtgtgccg accgtgaagg agaacccggc cggcccctcg
    12721 gcgagggcca gcccggtgtc cagggcggcc agcagggggc ggatctccgc cgggtcggga
    12781 gccgtcgccg acagccgcag caagggggtg acgggcagcc cggcggcggt ggggtggcgg
    12841 gaggcgcccc agtcgatgag gaagggcacc agcccggacg ggtgggcgga accgtccgtc
    12901 agccgccact ccagcagggc gccgtcgggc gtgcggcggc tcatcgcccg ggcgggaccg
    12961 ggatcgtggc cgcgggcgcg ggccgaggcg atcgccgcgt ccaggtcggt cgtgcggatc
    13021 gcccaggtca cggtgcgcgg gccgggcagc aggtccacgc cgaacggccg cgggccggcc
    13081 ggggcgggct gctccgggtc cgggccgatg atctccaggt acc
//
