LOCUS       BGC0000281              8137 bp    DNA     linear   BCT 28-JUN-2005
DEFINITION  Pseudomonas fluorescens strain 2P24 phl 2,4-DAPG gene cluster,
            complete sequence.
ACCESSION   BGC0000281
VERSION     BGC0000281
KEYWORDS    .
SOURCE      Pseudomonas fluorescens
  ORGANISM  Pseudomonas fluorescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:18:47
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(1..678)
                     /gene="phlH"
     CDS             complement(1..678)
                     /codon_start=1
                     /gene="phlH"
                     /gene_functions="regulatory (smcogs) SMCOG1057:TetR family
                     transcriptional regulator (Score: 84.6; E-value: 1.9e-25)"
                     /gene_kind="regulatory"
                     /note="similar to the IfeR of Agrobacterium tumefaciens
                     encoded by sequence deposited in GenBank Accession number
                     AF039653; similar to members of the TetR/AcrR family"
                     /product="PhlH"
                     /protein_id="AAY86545.1"
                     /transl_table=11
                     /translation="MDNAIGKPSLPRASRLDGQATRLQILEKAGELFAEQGLANTTSKQ
                     ICERSQANSAAVNYHFVNKEGLYRAVLLEAHARLVQLETLVSLNERPGSPQDKLRALIT
                     VLVERLHNHPDGWALKVLTREVLSPSPEFEVVLKEQSFPKAHILRGLLGQIMNLPADHP
                     TTLRSAISVFAPCLFLLIAHQPLKQHVLQGLSLEPQGLIDHMMSYALGGLQAVAATAHD
                     AAS"
     source          1..8137
                     /db_xref="taxon:294"
                     /mol_type="genomic DNA"
                     /organism="Pseudomonas fluorescens"
                     /strain="2P24"
     misc_feature    1..8137
                     /note="phl 2,4-DAPG gene cluster"
     protocluster    1..8137
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[5649:6738]"
                     /cutoff="20000"
                     /detection_rule="(Chal_sti_synt_C or Chal_sti_synt_N)"
                     /neighbourhood="20000"
                     /product="T3PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5650..6738
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(Chal_sti_synt_C or Chal_sti_synt_N)"
                     /neighbourhood="20000"
                     /product="T3PKS"
                     /protocluster_number="1"
     cand_cluster    1..8137
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Chal_sti_synt_C or Chal_sti_synt_N)"
                     /kind="single"
                     /product="T3PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..8137
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000281"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8137
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T3PKS"
                     /region_number="1"
                     /rules="(Chal_sti_synt_C or Chal_sti_synt_N)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            787..1707
                     /gene="phlG"
     CDS             787..1707
                     /codon_start=1
                     /gene="phlG"
                     /note="similar to the phlG of Pseudomonas fluorescens
                     strain CHA0, encoded by sequence deposited in GenBank
                     Accession Number AF0207529"
                     /product="PhlG"
                     /protein_id="AAY86546.1"
                     /transl_table=11
                     /translation="MLRLLTHYQGNIVMEARVMTPFTYFSLPMQKQFLANQQAVQGKPY
                     AEFFRSKISVPLSAVEKIQQGPMPLADTLTPSIEDLNRMLAADFISEEAGYALLPGPMA
                     YVQSRKFFPNCTAEMLKWWFMWHPLEAERYTLWFPYAHVENPCVHHERLSDTTLSFEES
                     LYGNTFCASEYVGDRLMHLHIHFRDPCELGFCPDLYRESKIDGSVSALMSLAHEPQVPV
                     SLMAHLFKECPEGLYLTSRYWVGSHPAMQRFPGAERAAQLLEESGLGEVELETLAYEFA
                     VHDMCEFNHLASILPSLHAQFSGAK"
     gene            complement(1727..2353)
                     /gene="phlF"
     CDS             complement(1727..2353)
                     /codon_start=1
                     /gene="phlF"
                     /gene_functions="regulatory (smcogs) SMCOG1224:TetR family
                     transcriptional regulator (Score: 168; E-value: 3.6e-51)"
                     /gene_kind="regulatory"
                     /note="transcriptional repressor of 2,4-DAPG biosynthesis"
                     /product="PhlF"
                     /protein_id="AAY86547.1"
                     /transl_table=11
                     /translation="MARKPSRSSIGSLRSPHTHKAIIISAIETLKECGYSGLSIEAVAR
                     RAGASKPTIYRWWGNKAALIAEVYESESEQIRKEPDKGSFKENLNFLLLNLWKVWRETI
                     CGEAFRCVIAEAQLDPSTLPKLKDEFMERRRELPRKLVENAIQQGELPKDTSRELLLDM
                     IFGFCWYRLLTEQLEVEADINEFTTLLLNGVLRPTSAAVQGAAEA"
     gene            2771..3853
                     /gene="phlA"
     CDS             2771..3853
                     /codon_start=1
                     /gene="phlA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1043:hydroxymethylglutaryl-CoA synthase (Score: 245.1;
                     E-value: 1.9e-74)"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to acyl carrier synthases;
                     2,4-diacetylphloroglucinoln (DAPG) biosynthesis"
                     /product="PhlA"
                     /protein_id="AAY86548.1"
                     /transl_table=11
                     /translation="MNKVGIVSYGAGIPVCRLKVDDVIQVWKNTDLSLVKGQLGVIERA
                     VLQPDEDVITLGVLAAQRALDEAPPCSLEALYLGTCTNPYDSRASAAIILEMLGCGYDA
                     FCADVQFAGKSGTSALQIAYALVASGMVGNALAVGADTINRNTAPGDLTESYAGAGAAA
                     LLLGTENVIAHFDASFSCAADVADNIRPQGDRYIRSGMGLGPDKNSIGLEDQTRRAASG
                     LMAKIHAQADDFDYVVFQQNLVSTPYSLGKHLGFTKAQIEPGIYAQSVGDAGAASPLLG
                     LVNVLDQARPGERILVVSYGFGAGSDAIALTVTDAIEAYQKTNVPLRTQLEDKYYVDYR
                     TSIKYEFKYLRPDYALTAYL"
     gene            3884..5080
                     /gene="phlC"
     CDS             3884..5080
                     /codon_start=1
                     /gene="phlC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to thiolase portion of SCO-x"
                     /product="PhlC"
                     /protein_id="AAY86549.1"
                     /sec_met_domain="ketoacyl-synt (E-value: 1.2e-09, bitscore:
                     27.2, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCARRVAIVSAAYTPKPGSSRVRQTFKEMIVESAYQALNAIKMHP
                     RELQAVAYGYHGEGISEYGGLGPTISDALGISPAPTFMSTANCTSSSVSFQMAHQMVAS
                     GEYDIVLCGGFEKMTDHFNYAEYIGSSTECEYDYFLGISHTDAFALATAEYFEKYGYAG
                     READVLATFGRQMRIYAHNTPTATRYGVPIPSLETLKSSEACGSMLAWGEASGCAILVA
                     EHLVHRYTTQPVFVRGCAYTGVSHYFGTRYHNPTLQFPGLPKDVGMAVSANSLACAEIA
                     YKKAGITAKDIDVAQVYDLLGAGLIQMESMGVCGPGQAGDFVLEGGIALDGQLPLNTDG
                     GNIGRGHASGCDGILHITELFRQLRGESNNQVKGARIGVSQSLGGYAAHNSVIVLSND"
     gene            5091..5531
                     /gene="phlB"
     CDS             5091..5531
                     /codon_start=1
                     /gene="phlB"
                     /product="PhlB"
                     /protein_id="AAY86550.1"
                     /transl_table=11
                     /translation="MSLYPEQIHRMTAASMLREWREHGGKYRLEGSRCQDCEEIFFPRR
                     TVCGACNSLNVEPYRCARTGTIQVSAHAENPILAAMGYGETVPRFMAMVRLDDGLVIAS
                     EIVDVIDPRQVVSGAPVRMVIRKHVRESNLAWQYAYKFVLVK"
     gene            5650..6738
                     /gene="phlD"
     CDS             5650..6738
                     /codon_start=1
                     /gene="phlD"
                     /gene_functions="biosynthetic (rule-based-clusters) T3PKS:
                     Chal_sti_synt_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:chalcone and stilbene synthase domain protein
                     (Score: 344.4; E-value: 9.8e-105)"
                     /gene_kind="biosynthetic"
                     /note="similar to chalcone synthase and stilbene synthases;
                     putative synthase"
                     /product="PhlD"
                     /protein_id="AAY86551.1"
                     /sec_met_domain="Chal_sti_synt_N (E-value: 5.4e-24,
                     bitscore: 73.9, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFLTLLAFSRGLVMSTLCKPSLLFPQYKITQQQMIEHLEQLHDDH
                     PRMALAKRMIQNTQVNERYLVLPIDELAVHTGFTHRSIAYEREARRMSSIAARQAIENA
                     GLTTDDIRMVAVTSCTGFMMPSLTAHLINDLGLRTSTVQLPIAQLGCVAGAAAINRAND
                     FASLAPDNHVLIVSLEFSSLCYQPQDTKLHAFISAALFGDAVSACVMRADDQASGFKIA
                     KTGSYFLPDSEHYIKYDVKDSGFHFTLDKAVMNSIKDVAPMMEELNYETFNQHCAQNDF
                     FIFHTGGREILDELVLQLDLEPGRVAQSRDSLSEAGNIASVVVFDVLKRQFDSGPVNGA
                     TGMLAAFGPGFTAEMAVGKWVA"
     gene            6866..8137
                     /gene="phlE"
     CDS             6866..8137
                     /codon_start=1
                     /gene="phlE"
                     /gene_functions="transport (smcogs) SMCOG1106:major
                     facilitator transporter (Score: 228.7; E-value: 1.6e-69)"
                     /gene_kind="transport"
                     /note="putative integral membrane protein"
                     /product="PhlE"
                     /protein_id="AAY86552.1"
                     /transl_table=11
                     /translation="MEMKYLVARPWGGYERRMITLLSLSFGLVGLDRFIIMPLFPVIMH
                     DLALDYQDLSMLSAILAFAWGGSALFMGAAIRVLGTKKLLVLSITLLSLLAGASALITS
                     LVGLVLLRALMGVCEGAFTPVSIIVTDEVSQPCRRGLNLGIQQALFPIIGLCLGPLLAG
                     VLLEVFGSWRAVFAIISLPGLLVAWYLYKTYQPTQAPSTASIARTSRSQWRTALSSGNV
                     RLNIALMLCILTCQFVLCALLPSYLTDILHLSNFSMSMIISAIGLGGFFGQLVIPGLSD
                     QLGRKPVVSICFMVSALLVGLLIISPALPWLLFLQLFFLSFINFSLICITVGPLTSESV
                     PPSLLATATGLVVGCGEILGGGVAPVVAGYIAMTWGLTAILFLALAGSLTGGLLSLWLK
                     EANPVFNGLKDYAPLSARLTLEDK"
ORIGIN
        1 tcagcttgca gcatcgtgcg cagttgccgc cactgcctgg aggccgccaa gggcatagct
       61 catcatgtgg tctatcaagc cctgcggctc gagactcaac ccctgcagta catgctgctt
      121 tagcggctga tgggctataa gcaggaaaag acagggtgcg aagacgctga tggcgctgcg
      181 caacgttgtc gggtgatccg ccggcaagtt catgatttgt ccaagcaagc cacgcaggat
      241 gtgtgccttg ggaaacgatt gctccttgag caccacctca aactcgggag aaggcgaaag
      301 cacttcgcgc gtgagcactt tcagcgccca accatcggga tgattgtgca gtcgctcgac
      361 cagcacggta atgagcgcac gcaacttgtc ctgcggcgaa cctggccgct cgttaagcga
      421 aaccagcgtt tccagttgca ccaaccgagc atgggcttcg agcaacactg cgcgatacag
      481 gccctcttta ttcacgaagt gatagttcac cgccgcactg ttggcttgcg aacgttcaca
      541 aatctgcttg ctggtggtgt tggccaaccc ctgctcggca aacaattcac ccgccttttc
      601 cagaatctgc agccgcgttg cctgcccatc gagccgcgaa gcacgaggca gggaaggctt
      661 gccaatggca ttgtccatga gctcatctct ttatagttct aattcaaatt tgacttttta
      721 tttcagccta cctattttgg tcccgtcatt gtccctttac aacaccgcgt gaggacttcg
      781 cagccgatgc tgcggcttct cacgcactat caaggaaata tcgtcatgga agcgcgcgta
      841 atgacacctt tcacctactt ctcattgcca atgcagaagc agttcctggc caatcaacag
      901 gccgttcaag gcaaacccta cgccgaattt ttccgctcga agatcagcgt gccgctcagt
      961 gctgtcgaaa aaatacagca aggccccatg ccgctggctg acaccttgac gcccagtatt
     1021 gaagacctca accgcatgct cgctgctgat ttcattagcg aagaggcagg ctacgccttg
     1081 ctgccagggc cgatggctta tgtgcaaagc cgcaagtttt ttcccaactg cacagctgag
     1141 atgctcaagt ggtggttcat gtggcatcca ttggaggctg aacgctacac cttgtggttt
     1201 ccttatgccc atgtggaaaa cccctgcgtt catcacgagc gtctcagcga cactaccttg
     1261 agctttgagg aaagcttgta tgggaatacc ttttgcgcct ctgagtacgt cggcgaccgt
     1321 ctcatgcacc tacacattca tttcagggat ccctgcgagc tgggcttctg tccggatctg
     1381 tatcgcgaat caaaaataga cggcagcgtc agcgccttga tgagcctggc ccacgaaccg
     1441 caagtgccgg tgtcgctgat ggcgcacttg ttcaaggaat gcccggaagg tttgtacctg
     1501 acgagccgtt actgggtcgg cagccaccct gccatgcagc gtttcccagg cgccgagcga
     1561 gccgcccagc tcctggaaga aagcggcctg ggggaagtgg agttggaaac cctggcttat
     1621 gaatttgcag tgcatgacat gtgcgagttc aatcacctgg ccagcatcct gccgagcctt
     1681 catgcgcaat tcagtggcgc aaagtaagga caaccccacc cgataatcag gcttcggcgg
     1741 cgccctgaac cgctgccgaa gtcgggcgca ataccccgtt cagcagaagt gtcgtgaact
     1801 cattgatatc cgcttccacc tccaactgct cagtcaacag ccgataccag cagaacccaa
     1861 agatcatgtc caaaagcagt tcacgcgacg tatcctttgg caactcgcct tgctgaatgg
     1921 cattttccac cagcttgcga ggcaactccc tacgccgctc catgaattca tctttcagtt
     1981 ttggcagcgt actgggatcg agttgggcct cagcgatgac gcaccgaaaa gcctccccgc
     2041 aaatcgtttc tcgccagacc ttccatagat tgagcagcaa gaaattgagg ttctccttga
     2101 aggagccttt atcaggctct ttgcgaatct gctcgctttc actctcgtag acctcggcga
     2161 tcaaggctgc cttgttaccc caccaccgat agatggtcgg tttactcgcg ccggcacggc
     2221 gagccaccgc ctcgatgctc aaccccgaat aaccgcactc cttaagggtt tctatggcgg
     2281 aaataatgat cgctttatgc gtgtgagggc tcctcaatga gccaatggag ctccgagatg
     2341 gtttacgggc cataattcaa aatctccaat atggcgcttg acaaaagaga ctcagggtat
     2401 aggattctct cgaaaagcaa tgaaacggat cgtttcgttt tgtatgttta tttgatctca
     2461 gcttctgctt agcagcgggc ctctggagcg cttattgcct ctatgtaggg aaatcggaga
     2521 aggaaagtca taaaatttta acgcaacctt aacgatacgg cacgtttcgt atcatacata
     2581 acaattgtac aagaataaac ccattgttca catgctgacc ggtaggttcc gccttgtgaa
     2641 cctgcctatt tacgggcata ggagcgccag tatcactttg cgggcaccaa tagatagttt
     2701 ctaaatattt catgcgcttg tcacggcccc cggccaacag gtgaaagaac ggaatctgga
     2761 ggatgtacac atgaataagg taggaattgt gagctatggc gcgggtatcc cggtctgccg
     2821 gctcaaggtg gacgacgtga tccaggtgtg gaaaaacacc gatctgagtt tggtaaaggg
     2881 ccaattgggc gttattgaaa gggccgtcct gcaacccgac gaagatgtca taaccttggg
     2941 tgtcctcgcc gcccaacgcg cgcttgacga ggcaccacct tgttccctcg aagcgcttta
     3001 tctcggcacc tgcaccaatc cttatgactc ccgggcctcg gccgcaatca tcctggaaat
     3061 gctcggctgc ggctatgacg ccttttgcgc agacgtgcag tttgccggca aatcaggcac
     3121 cagcgccctc cagatcgcat acgccttggt agcgtcgggc atggtgggca acgctttggc
     3181 ggtgggtgcc gatacgatca accgcaacac cgcccccggt gacttgacgg agtcctatgc
     3241 cggagccggg gccgcggcct tgctgttggg gacagaaaat gtgatcgcgc attttgacgc
     3301 aagtttttct tgcgcggcgg atgtcgctga caacatcagg cctcaagggg accgctatat
     3361 ccgctcggga atgggattgg gcccggacaa gaacagtatc ggcctcgagg accagactcg
     3421 ccgcgccgcc tcgggattga tggccaagat ccatgcgcaa gccgatgatt tcgattacgt
     3481 cgtcttccaa caaaacctgg tgtcgacccc ctactctctc ggcaagcacc tgggattcac
     3541 gaaggcccaa atcgaacctg gcatctacgc ccagagtgtc ggcgatgctg gagccgcgag
     3601 tcctttactg ggcttggtca acgtactcga tcaagcgcgt cctggcgaaa gaatcctggt
     3661 ggtttcctat ggcttcggtg cgggcagcga tgccatcgcc ttgaccgtca ccgatgccat
     3721 cgaagcctat caaaagacca acgttccgct gcgcacgcag ctggaggata agtactacgt
     3781 ggattacagg acgtcgatca agtacgagtt caaatacttg cggcccgact atgccctgac
     3841 ggcctatctc tgattccaat cattcagcaa ggagcacctt tttatgtgcg cacgacgcgt
     3901 tgctatcgtt tcggctgcct atacccctaa acctggcagt tccagagttc gccagacttt
     3961 caaggagatg atcgtcgagt ccgcctacca ggcactcaac gccatcaaga tgcaccctcg
     4021 cgaacttcag gcagttgcct atgggtacca tggtgaaggt atttctgaat acggaggtct
     4081 gggcccaacc atctccgatg ccttggggat cagccctgcc cccacgttca tgagtacggc
     4141 caactgcacc agcagttcgg tgtctttcca gatggctcac caaatggtcg cctcaggtga
     4201 atacgacatc gttctgtgtg gcggctttga aaaaatgacc gatcacttca actatgccga
     4261 gtacattggc tcgagcactg aatgtgaata cgattatttt ctaggcatct ctcacacaga
     4321 cgccttcgcc ctggcgaccg ccgaatattt tgaaaagtac ggctatgcag gacgcgaagc
     4381 ggatgtattg gccactttcg ggcggcagat gcgcatttat gcccataaca ctcccacggc
     4441 tacccgttat ggcgtgccga tcccgtctct cgaaacactc aaaagcagcg aagcatgcgg
     4501 ctccatgctg gcctggggtg aagccagcgg ttgcgccatc ctggttgccg aacaccttgt
     4561 gcaccgctac acgacccaac cggtatttgt ccgtggctgt gcctacaccg gcgtgtcgca
     4621 ttatttcggc acgcgctatc acaacccgac gttgcaattt ccggggctgc caaaagatgt
     4681 cggcatggcg gtttccgcca actcgctggc ctgcgccgaa atcgcctaca aaaaagccgg
     4741 tatcaccgca aaggatatcg atgtggcgca agtctacgac ttgctgggcg ccgggttgat
     4801 tcaaatggag tccatgggcg tatgtggtcc aggccaggca ggcgactttg tattggaagg
     4861 cggcatagcc cttgatgggc aacttcccct gaacactgac ggcggcaaca tcggacgcgg
     4921 ccacgcttcc ggttgcgatg ggattttgca cattactgaa ttgttccggc agcttcgcgg
     4981 cgagtccaac aatcaagtca aaggcgcgcg tattggcgtc tcgcaaagcc tgggcggtta
     5041 cgccgcgcac aacagcgtca ttgtgctttc caacgattaa ggagccgacc atgtcccttt
     5101 accctgaaca aattcaccgc atgaccgccg ccagcatgtt gcgcgaatgg cgcgaacacg
     5161 gtggcaaata ccgcctcgaa ggcagccgat gccaggactg cgaagaaatc ttcttccctc
     5221 ggcgcacagt ttgcggtgcg tgcaactcgt tgaatgtaga accgtatcgc tgcgcgcgca
     5281 ccggcactat ccaggtcagc gcacacgccg agaaccccat tcttgcggcc atgggctacg
     5341 gcgaaaccgt gccccgcttc atggcgatgg tgcgcctgga tgatggtctg gtgattgcct
     5401 cggaaatagt tgacgtcatc gatccgcgac aagtcgtcag cggtgcgccc gtgcgcatgg
     5461 tcattcgaaa acatgtccgg gaaagtaact tggcctggca atatgcctat aagtttgtat
     5521 tggtgaaata acaaacttag acgccgccat accccgcgtt atcgataaca acagtcaagt
     5581 ttttggcctc caagcccgtt gctttgattc tgtctttcag agtccagctt tgctgttatt
     5641 gccttaaaca tgtttctaac tttattggct tttagccgag gacttgtcat gtctactctt
     5701 tgcaaaccca gtctgctgtt cccgcaatat aagatcaccc agcaacaaat gatcgagcat
     5761 ctagagcagt tgcatgatga tcacccgaga atggctcttg ccaaacgcat gattcaaaac
     5821 acacaggtga acgaacgata cttggtcctg cccattgatg aactggcggt gcataccggc
     5881 ttcacccatc gcagcattgc ttatgagcgc gaggcccgcc gcatgtcatc catcgcggcg
     5941 cgccaagcca tcgagaatgc cgggctgacc accgacgaca ttcgaatggt cgctgtgaca
     6001 tcgtgcacgg gtttcatgat gccttcgctg acggcccact tgatcaacga cctgggcctg
     6061 cgaacatcga ccgtacaact gcctatcgct caattgggat gcgtggcagg cgctgcggcg
     6121 atcaatcgag ccaatgactt cgccagcctg gcgccggaca accatgtgct catcgtctcc
     6181 ctggagttct cgtcgctctg ctatcaaccc caggacacca agttgcacgc attcatctca
     6241 gcggcattat tcggcgatgc ggtatcagcc tgcgtaatgc gagccgacga tcaggcgtct
     6301 ggtttcaaga tcgccaagac ggggtcttac ttcctgcctg atagcgagca ctatattaaa
     6361 tacgacgtca aagacagtgg ctttcacttc accctggaca aggcggtcat gaactccatt
     6421 aaagatgtcg cacccatgat ggaggaattg aactacgaga ccttcaatca acattgcgct
     6481 caaaatgact ttttcatctt ccatacaggc ggacgggaaa ttcttgatga actggtcctg
     6541 caattggact tggaacccgg cagggtcgcg caatcccgcg acagtttgag cgaagccggg
     6601 aacatcgcca gcgtggtggt cttcgatgtg ctcaagcgcc agttcgacag cggacctgtc
     6661 aatggcgcga cgggcatgtt ggcggccttc ggcccgggtt tcaccgctga aatggccgtg
     6721 ggcaagtggg tcgcctgaca gaccggataa aacaccagca cccagccagg ggcacacctg
     6781 cccctggcta cgggcgtatt gatcgcaggc gttcaacctc gttcgagaga gcttcgaaag
     6841 cgctagggaa agaaggggaa agaacatgga gatgaagtat ctggtcgctc gaccttgggg
     6901 cggctatgaa agacgcatga tcacgctttt gtcgctgagc ttcgggctgg taggactgga
     6961 caggttcatc atcatgccgc tgttcccggt gatcatgcat gacttggccc tggactatca
     7021 ggacttgagc atgctgtcgg ccatcctggc atttgcatgg ggaggctcgg ccctgttcat
     7081 gggtgccgcc atcagggtat tgggcaccaa gaagttgctg gtgctgtcga tcaccctgct
     7141 atccctcctg gccggtgcgt ccgcattgat caccagcctg gtgggcctgg tgctcttgcg
     7201 tgccttgatg ggcgtctgtg aaggtgcatt caccccggtc agtatcattg ttaccgatga
     7261 agtctcgcag ccctgccgac gcggactcaa tctgggtatt caacaggcat tgttccccat
     7321 catcggcctg tgcctcggac cgctgctggc gggcgtcttg ctggaagtgt tcggatcctg
     7381 gcgtgccgtg ttcgccatca tttcgttacc cggcctgtta gtggcctggt atctctataa
     7441 aacttaccaa cccacccagg cgcccagcac ggcgtcgatt gctcgcacct ccagaagcca
     7501 gtggaggaca gccctgtcca gcggcaatgt gcggctcaat attgccttga tgctgtgcat
     7561 cctgacgtgc cagttcgtgc tgtgtgccct gctccccagc tacctgaccg acattctgca
     7621 cctgagcaat ttctccatgt ccatgatcat ttccgccatt ggcctgggtg gtttctttgg
     7681 ccaactggtc attcccggct tgtccgacca actgggtcgc aagcccgtgg tttccatctg
     7741 cttcatggtc agcgcattgt tagtgggctt attgatcatc agccctgcgc tgccctggct
     7801 actgtttctt caattatttt tcttgtcctt catcaacttc agcctgatct gcataacggt
     7861 tggcccgctg accagcgaat cagtaccgcc cagtttattg gccaccgcga cagggctggt
     7921 ggtgggctgc ggagagatcc tgggcggagg cgtcgcgcct gtcgtcgccg gctacatcgc
     7981 catgacgtgg ggcctcaccg caatcctgtt cctggcactg gccggaagtt tgaccggtgg
     8041 gttgctgtcc ttgtggctca aagaggccaa cccggtcttc aacgggctca aggactatgc
     8101 ccccctatcc gcacgcttga ccctggagga taagtaa
//
