Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000289.1:
Cluster 1NRP / Saccharide189153

BGC0000289, A40926 biosynthetic gene cluster from Nonomuraea sp. ATCC. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: A40926
ChemSpider ID: 32741751
SMILES string: Copy to clipboard
Molecular formula:
Average molecular mass: Da
Molecular activity: Antibacterial
Molecular target: lipid II

Class-specific details

Biosynthetic class(es):
NRP / Saccharide
Glycopeptide (linear)
Thioesterase type:
Type I
Release / cyclization type:
Hydrolysis

Nonribosomal peptide synthetases:
dbv25 (CAD91220.1)
Module 1
A specificity: Hydroxyphenylglycine
Evidence for specificity: Sequence-based prediction
AA is epimerized
C domain subtype: N/A
Module 2
A specificity: Tyrosine
Evidence for specificity: Sequence-based prediction
AA is epimerized
C domain subtype: Other
dbv26 (CAD91221.1)
Module 3
A specificity: 3,5-Dihydroxyphenylglycine
Evidence for specificity: Sequence-based prediction
C domain subtype: Other
dbv17 (CAD91212.1)
Module 4
A specificity: Hydroxyphenylglycine
Evidence for specificity: Sequence-based prediction
AA is epimerized
C domain subtype: Other
Module 5
A specificity: Hydroxyphenylglycine
Evidence for specificity: Sequence-based prediction
AA is epimerized
C domain subtype: Other
Module 6
A specificity: Tyrosine
Evidence for specificity: Sequence-based prediction
C domain subtype: Other
dbv16 (CAD91211.1)
Module 7
A specificity: 3,5-Dihydroxyphenylglycine
Evidence for specificity: Sequence-based prediction
C domain subtype: Other
Scaffold-modifying domain: Other
Saccharide subclass:
hybrid/tailoring
Glycosyltransferases:
CAD91204.1:
Specificity: N-acetyl-D-glucosamine
Evidence for specificity: Activity assay

Gene cluster description

A40926 (BGC0000289). Gene Cluster 1. Biosynthetic class = NRP/Saccharide. GenBank AJ561198, positions 6242-77096. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Gene expression correlated with compound production, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Stefano Donadio (Naicons)

Literature references

1. Sosio M et al. (2003) The gene cluster for the biosynthesis of the glycopeptide antibiotic A40926 by nonomuraea species. Chem Biol 10(6):541-9.
2. Kruger RG et al. (2005) Tailoring of glycopeptide scaffolds by the acyltransferases from the teicoplanin and A-40,926 biosynthetic operons. Chem Biol 12(1):131-40. doi: 10.1016/j.chembiol.2004.12.005.
3. Donadio S et al. (2005) Comparative analysis and insights into the evolution of gene clusters for glycopeptide antibiotic biosynthesis. Mol Genet Genomics 274(1):40-50. doi: 10.1007/s00438-005-1156-3. Epub
4. Stinchi S et al. (2006) A derivative of the glycopeptide A40926 produced by inactivation of the beta-hydroxylase gene in Nonomuraea sp. ATCC39727. FEMS Microbiol Lett 256(2):229-35. doi:
5. Ho JY et al. (2006) Glycopeptide biosynthesis: Dbv21/Orf2 from dbv/tcp gene clusters are N-Ac-Glm teicoplanin pseudoaglycone deacetylases and Orf15 from cep gene cluster is a Glc-1-P thymidyltransferase. J Am Chem Soc 128(42):13694-5. doi: 10.1021/ja0644834.
6. Li YS et al. (2007) A unique flavin mononucleotide-linked primary alcohol oxidase for glycopeptide A40926 maturation. J Am Chem Soc 129(44):13384-5. doi: 10.1021/ja075748x. Epub 2007 Oct
7. Sosio M et al. (2010) Improved production of A40926 by Nonomuraea sp. through deletion of a pathway-specific acetyltransferase. Appl Microbiol Biotechnol 87(5):1633-8. doi: 10.1007/s00253-010-2579-2.
8. Alduina R et al. (2007) Phosphate-controlled regulator for the biosynthesis of the dalbavancin precursor A40926. J Bacteriol 189(22):8120-9. doi: 10.1128/JB.01247-07. Epub 2007 Sep 14.