BGC0000303 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000303 (Streptomyces refuineus subsp. thermotolerans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS-like,NRPS	1	33,150	High	anthramycin	NRPS:Type I

BGC0000303 - Region 1 - NRPS,NRPS-like

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Location: 1 - 33,150 nt. (total: 33,150 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 33,151
Label		BGC0000303

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase oxygenase

ABW71833.1: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

ABW71834.1: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

ABW71835.1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ABW71838.1: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

ABW71845.1: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

ABW71854.1: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

ABW71855.1: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Transferases: aminotransferase methyltransferase

ABW71836.1: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

ABW71847.1: aminotransferase class V

smcogs: SMCOG1139: aminotransferase class V

ABW71850.1: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Hydrolases: hydrolase peptidase

ABW71832.1: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

ABW71837.1: gamma-glutamyltranspeptidase

smcogs: SMCOG1274: gamma-glutamyltranspeptidase

ABW71851.1: alpha/beta hydrolase domain-containing protein

smcogs: SMCOG1066: alpha/beta hydrolase domain-containing protein

Ligases: ligase

ABW71852.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ABW71853.1: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000303.5	1.68	NRPS	anthramycin	Streptomyces refuineus subsp. thermotolerans
BGC0001135.5	0.60	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002164.2	0.59	NRPS	peramine	Epichloe festucae
BGC0000307.4	0.59	NRPS	AbT1	Aureobasidium pullulans
BGC0001261.3	0.58	NRPS	AM-toxin	Alternaria alternata
BGC0000357.5	0.58	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001166.5	0.58	NRPS	HC-toxin	Alternaria jesenskae
BGC0001240.4	0.58	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0000342.4	0.58	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.58	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000303.5	0.99	NRPS	anthramycin	Streptomyces refuineus subsp. thermotolerans
BGC0001135.5	0.63	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002164.2	0.63	NRPS	peramine	Epichloe festucae
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0001261.3	0.63	NRPS	AM-toxin	Alternaria alternata
BGC0000357.5	0.63	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001166.5	0.63	NRPS	HC-toxin	Alternaria jesenskae
BGC0001240.4	0.63	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0000342.4	0.63	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 2609 - 33150:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 4413 - 33150:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ABW71852.1: X

AMP-binding (26..417): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AATNISAALK
Nearest Stachelhaus code(s):: mxAnt AATHLSATLK (82% 8Å match)
Stachelhaus code match:: 70% (weak)

ABW71853.1: X

AMP-binding (472..872): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLYYTALVK
Nearest Stachelhaus code(s):: proPro DVLYYTALCK (91% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor