Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000305.1:
Cluster 1NRP140430

BGC0000305, arthrofactin biosynthetic gene cluster from Pseudomonas sp. MIS38. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Arthrofactin A
SMILES string: Copy to clipboard
Molecular formula: C64H111N11O20
Exact molecular mass: 1353.8007 Da (M+)
Molecular activity: Surfactant

Class-specific details

Biosynthetic class(es):
NRP
Nonribosomal peptide subclass:
Other lipopeptide (cyclic)
3-OH-decanoic acid
Thioesterase type:
Type I & II
Release / cyclization type:
Macrolactonization

Nonribosomal peptide synthetases:
arfA (BAC67534.2)
Module 2
A specificity: Aspartate
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: Dual
Module 1
A specificity: Leucine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: Starter
arfB (BAC67535.1)
Module 3
A specificity: Threonine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: Dual
Module 4
A specificity: Leucine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: Dual
Module 5
A specificity: Leucine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: Dual
Module 6
A specificity: Serine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: Dual
arfC (BAC67536.1)
Module 7
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module 8
A specificity: Serine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: LCL
Module 9
A specificity: Isoleucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module 10
A specificity: Isoleucine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 11
A specificity: Aspartate
Evidence for specificity: Structure-based inference
C domain subtype: LCL

Gene cluster description

arthrofactin (BGC0000305). Gene Cluster 1. Biosynthetic class = NRP. GenBank AB107223. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Harald Gross (University of Tuebingen)

Literature references

1. Lange A et al. (2012) Predicting the structure of cyclic lipopeptides by bioinformatics: structure revision of arthrofactin. Chembiochem 13(18):2671-5. doi: 10.1002/cbic.201200532. Epub 2012
2. Washio K et al. (2010 TI) Identification and characterization of the genes responsible for the production of the cyclic lipopeptide arthrofactin by Pseudomonas sp. MIS38. Biosci Biotechnol Biochem 74(5):992-9. doi: 10.1271/bbb.90860. Epub 2010
3. Roongsawang N et al. (2003) Cloning and characterization of the gene cluster encoding arthrofactin synthetase from Pseudomonas sp. MIS38. Chem Biol 10(9):869-80.