Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000319.1:
Cluster 1NRP134452

BGC0000319, cephamycin C biosynthetic gene cluster from Streptomyces clavuligerus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: cephamycin C
SMILES string: Copy to clipboard
Molecular activity: Antibacterial, Inhibitor
Molecular target: penicillin binding proteins, transpeptidase, carboxypeptidase

Class-specific details

Biosynthetic class(es):
NRP
Beta-lactam (cyclic)
Thioesterase type:
Unknown
Release / cyclization type:
Unknown

Nonribosomal peptide synthetases:
pcbAB (EDY47118.1)
Module 0
A specificity: 2-Amino-adipic-acid
Evidence for specificity: Activity assay
C domain subtype: N/A
Module 1
A specificity: Cysteine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 2
A specificity: Valine
Evidence for specificity: Activity assay
AA is epimerized
C domain subtype: LCL

Gene cluster description

cephamycin C (BGC0000319). Gene Cluster 1. Biosynthetic class = NRP. GenBank DS570624, positions 142995-177446. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Susan Jensen (University of Alberta)

Literature references

1. Leskiw BK et al. (1988 TI) Cloning and nucleotide sequence determination of the isopenicillin N synthetase gene from Streptomyces clavuligerus. Gene 62(2):187-96.
2. Petrich AK et al. (1992) Transcriptional analysis of the isopenicillin N synthase-encoding gene of Streptomyces clavuligerus. Gene 111(1):77-84.
3. Durairaj M et al. (1992) High-level expression of the Streptomyces clavuligerus isopenicillin N synthase gene in Escherichia coli. Appl Environ Microbiol 58(12):4038-41.
4. Durairaj M, Jensen SE. (1996) Purification and characterization of recombinant Streptomyces clavuligerus isopenicillin N synthase produced in Escherichia coli. J Ind Microbiol 16(3):197-203.
5. Paradkar AS et al. (1996) Molecular analysis of a beta-lactam resistance gene encoded within the cephamycin gene cluster of Streptomyces clavuligerus. J Bacteriol 178(21):6266-74.
6. Alexander DC, Jensen SE. (1998) Investigation of the Streptomyces clavuligerus cephamycin C gene cluster and its regulation by the CcaR protein. J Bacteriol 180(16):4068-79.
7. Alexander DC et al. (2000) Early cephamycin biosynthetic genes are expressed from a polycistronic transcript in Streptomyces clavuligerus. J Bacteriol 182(2):348-56.
8. Trepanier NK et al. (2002) The positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus is mistranslated in a bldA mutant. Microbiology 148(Pt 3):643-56. doi: 10.1099/00221287-148-3-643.
9. Wang L et al. (2004) Transcriptional and translational analysis of the ccaR gene from Streptomyces clavuligerus. Microbiology 150(Pt 12):4137-45. doi: 10.1099/mic.0.27245-0.
10. Alexander DC et al. (2007) pcd mutants of Streptomyces clavuligerus still produce cephamycin C. J Bacteriol 189(16):5867-74. doi: 10.1128/JB.00712-07. Epub 2007 Jun