BGC0000319: cephamycin C biosynthetic gene cluster from Streptomyces clavuligerus ATCC 27064
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
View antiSMASH-generated output
Download Cluster GenBank file
Download region SVG
Location: 142,995 - 194,661 nt. (total: 51,667 nt).
This entry is originally from NCBI GenBank DS570624.1.

Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
reset zoomreset view
zoomzoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
General
Compounds
Genes
Biosynthesis
History
KnownClusterBlast
NRPS/PKS domains
General information about the BGC
MIBiG accession BGC0000319
Short description cephamycin C biosynthetic gene cluster from Streptomyces clavuligerus ATCC 27064
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
Loci
DS570624.1
142995 - 177446
via Knock-out studies, Enzymatic assays, Heterologous expression
Compounds
  • cephamycin C
Species Streptomyces clavuligerus ATCC 27064 [taxonomy]
References
Chemical products information
cephamycin C Evidence:
Copy SMILES
C16H22N4O9S1
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • SSCG_00130
  • EDY47102.1
 142995 - 143993 (+) beta-lactamase
    copy AA seq
    copy Nt seq
    • SSCG_00131
    • EDY47103.1
    		 144030 - 146111 (-) D-alanyl-D-alanine carboxypeptidase
      copy AA seq
      copy Nt seq
      • SSCG_00132
      • EDY47104.1
      		 146465 - 147934 (-) cephamycin export protein cmcT
        copy AA seq
        copy Nt seq
        • SSCG_00133
        • EDY47105.1
        		 148322 - 149860 (-) aldehyde dehydrogenase
          copy AA seq
          copy Nt seq
          • SSCG_00134
          • EDY47106.1
          		 149876 - 150811 (-) deacetoxycephalosporin C synthetase
            copy AA seq
            copy Nt seq
            • SSCG_00135
            • EDY47107.1
            		 150890 - 152086 (-) isopenicillin N epimerase
              copy AA seq
              copy Nt seq
              • SSCG_00136
              • EDY47108.1
              		 152449 - 152568 (-) hypothetical protein
                copy AA seq
                copy Nt seq
                • SSCG_00137
                • EDY47109.1
                		 152861 - 153571 (+) conserved hypothetical protein
                  copy AA seq
                  copy Nt seq
                  • SSCG_00138
                  • EDY47110.1
                  		 153620 - 154552 (+) 7-alpha-cephem-methoxylase P8 chain
                    copy AA seq
                    copy Nt seq
                    • SSCG_00139
                    • EDY47111.1
                    		 154649 - 155605 (+) deacetoxycephalosporin C hydroxylase
                      copy AA seq
                      copy Nt seq
                      • SSCG_00140
                      • EDY47112.1
                      		 155681 - 157246 (+) 3'-hydroxymethylcephem-O-carbamoyltransferase
                        copy AA seq
                        copy Nt seq
                        • SSCG_00141
                        • EDY47113.1
                        		 158162 - 158950 (+) positive regulator
                          copy AA seq
                          copy Nt seq
                          • SSCG_00142
                          • EDY47114.1
                          		 159019 - 159195 (-) hypothetical protein
                            copy AA seq
                            copy Nt seq
                            • SSCG_00143
                            • EDY47115.1
                            		 159273 - 160289 (+) secreted protein
                              copy AA seq
                              copy Nt seq
                              • SSCG_00144
                              • EDY47116.1
                              		 160636 - 161184 (+) BLP
                                copy AA seq
                                copy Nt seq
                                • SSCG_00145
                                • EDY47117.1
                                		 161790 - 163163 (+) L-lysine-epsilon aminotransferase
                                  copy AA seq
                                  copy Nt seq
                                  • SSCG_00146
                                  • EDY47118.1
                                  		 163400 - 174661 (+) N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D- valine synthase
                                    copy AA seq
                                    copy Nt seq
                                    • SSCG_00147
                                    • EDY47119.1
                                    		 174693 - 175682 (+) isopenicillin N synthetase
                                      copy AA seq
                                      copy Nt seq
                                      • SSCG_00148
                                      • EDY47121.1
                                      		 175791 - 177446 (-) secreted penicillin binding protein
                                        copy AA seq
                                        copy Nt seq
                                        • SSCG_00149
                                        • EDY47120.1
                                        		 177438 - 177650 (+) hypothetical protein
                                          copy AA seq
                                          copy Nt seq
                                          Biosynthesis information

                                          Biosynthetic modules

                                          Name
                                          0
                                          Type
                                          nrps-type1
                                          Genes
                                          SSCG_00146
                                          Substrates
                                          2-aminoadipic acid (evidence: Activity assay)
                                          Integrated Monomers
                                          Domains
                                          adenylation
                                          Name
                                          1
                                          Type
                                          nrps-type1
                                          Genes
                                          SSCG_00146
                                          Substrates
                                          cysteine (evidence: Activity assay)
                                          Integrated Monomers
                                          Domains
                                          condensation (DCL), adenylation
                                          Name
                                          2
                                          Type
                                          nrps-type1
                                          Genes
                                          SSCG_00146
                                          Substrates
                                          valine (evidence: Activity assay)
                                          Integrated Monomers
                                          Domains
                                          condensation (LCL), adenylation, epimerase
                                          Annotation changelog

                                          Entry version: 4

                                          Date
                                          Changes
                                          Submitters
                                          Reviewers
                                          2022-10-07
                                          Update chemical activity to schema version 2.11
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                          Entry version: 3

                                          Date
                                          Changes
                                          Submitters
                                          Reviewers
                                          2022-09-15
                                          Corrected NRP module activity
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          2022-09-15
                                          Updated NRP substrate specificities
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                          Entry version: 2

                                          Date
                                          Changes
                                          Submitters
                                          Reviewers
                                          2019-10-16
                                          Migrated from v1.4
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          2019-10-16
                                          Updated compound(s) information (NPAtlas curation)
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                          Entry version: 1

                                          Date
                                          Changes
                                          Submitters
                                          Reviewers
                                          2015-06-12
                                          Submitted
                                          • (ID: SDHXYXULZ6ZTMHSITTYHRAHA)
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          Similar known clusters from MIBiG 4.0
                                          Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
                                          Detailed help and explanations are available here.

                                          Click on reference genes to show details of similarities to genes within the current region.
                                          Double click on a reference drawing to reverse the display of the genes.

                                          Click on an accession to open that entry in the MIBiG database.


                                          Location:

                                          Identifier
                                          Identity
                                          Coverage
                                          Detailed domain annotation
                                          Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
                                          A domain glossary is available here, and an explanation of the visualisation is available here.
                                          Selected features only
                                          Show module domains