BGC0000322 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000322 (Amycolatopsis orientalis)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS	1	34,244	chloroeremomycin	NRPS:Type I	100%

BGC0000322 - Region 1 - NRPS

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Location: 1 - 34,244 nt. (total: 34,244 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 34,245
Label		BGC0000322

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000322	1.00	NRP	chloroeremomycin	Amycolatopsis orientalis
BGC0001133	0.38	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000311	0.38	NRP	balhimycin	Amycolatopsis balhimycina DSM 5908
BGC0001459	0.36	NRP	decaplanin	Amycolatopsis regifaucium
BGC0001460	0.36	NRP	decaplanin	Amycolatopsis decaplanina DSM 44594
BGC0002710	0.36	NRP	metachelin C, metachelin A, metachelin A-CE, metachelin B, dimerumic acid 11-mannoside, dimerumic acid	Metarhizium robertsii ARSEF 23
BGC0001461	0.35	NRP	nogabecin	Amycolatopsis keratiniphila subsp. nogabecina
BGC0001629	0.35	NRP	jessenipeptin	Pseudomonas sp. QS1027
BGC0000351	0.34	NRP	exochelin	Mycolicibacterium smegmatis MC2 155
BGC0002437	0.33	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000322	1.00	NRP	chloroeremomycin	Amycolatopsis orientalis
BGC0001133	0.71	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000311	0.70	NRP	balhimycin	Amycolatopsis balhimycina DSM 5908
BGC0001459	0.69	NRP	decaplanin	Amycolatopsis regifaucium
BGC0001460	0.69	NRP	decaplanin	Amycolatopsis decaplanina DSM 44594
BGC0002710	0.69	NRP	metachelin C, metachelin A, metachelin A-CE, metachelin B, dimerumic acid 11-mannoside, dimerumic acid	Metarhizium robertsii ARSEF 23
BGC0001461	0.68	NRP	nogabecin	Amycolatopsis keratiniphila subsp. nogabecina
BGC0001629	0.68	NRP	jessenipeptin	Pseudomonas sp. QS1027
BGC0000351	0.68	NRP	exochelin	Mycolicibacterium smegmatis MC2 155
BGC0002437	0.66	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 34244:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Leu - D-bOH-Tyr - Asn) + (D-Hpg - D-Hpg - bOH-Tyr) + (Dhpg)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CAA11794.1

Search NORINE for peptide: strict or relaxed

AMP-binding (16..403): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAFYLGMMCK
Nearest Stachelhaus code(s):: Leu DAFYLGMMCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1038..1424): bOH-Tyr

nrpys: bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: bht (bOH-Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DTSKVAAICK
Nearest Stachelhaus code(s):: bOH-Tyr DTSKVAAICK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2545..2949): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKLGEVGK
Nearest Stachelhaus code(s):: Asn DLTKLGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

CAA11795.1

Search NORINE for peptide: strict or relaxed

AMP-binding (460..855): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: hpg (Hpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DIFHLGLLCK
Nearest Stachelhaus code(s):: Hpg DIFHLGLLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1960..2352): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: hpg (Hpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DAVHLGLLCK
Nearest Stachelhaus code(s):: Hpg DAVHLGLLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3476..3863): bOH-Tyr

nrpys: bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: bht (bOH-Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTLGAICK
Nearest Stachelhaus code(s):: bOH-Tyr DASTLGAICK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

CAA11796.1

Search NORINE for peptide: strict or relaxed

AMP-binding (470..865): Dhpg

nrpys: Dhpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: dhpg (Dhpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DPYHGGTLCK
Nearest Stachelhaus code(s):: Dhpg DPYHGGTLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)