LOCUS       BGC0000328              6497 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Pseudomonas savastanoi pv. glycinea plasmid p4180A CmaD, CmaE, CmaA,
            CmaB, CmaC, CmaT, alanyl tRNA synthetase related protein, and CmaU
            genes, complete cds.
ACCESSION   BGC0000328
VERSION     BGC0000328
KEYWORDS    .
SOURCE      Pseudomonas savastanoi pv. glycinea (Pseudomonas syringae pv.
            glycinea)
  ORGANISM  Pseudomonas savastanoi pv. glycinea
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 13:24:04
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6497
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..231
                     /NRPS_PKS="Domain: PP-binding (4-55). E-value: 2.4e-14.
                     Score: 45.6. Matches aSDomain:
                     nrpspksdomains_AAQ93482.1_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /note="putative acyl carrier protein (ACP); contains
                     phosphopantetheine attachment site; required for coronatine
                     production"
                     /product="CmaD"
                     /protein_id="AAQ93482.1"
                     /sec_met_domain="PP-binding (E-value: 3.4e-15, bitscore:
                     45.6, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSAKLDQIFEAIFQRPVENDEDIFDLGANSLTAIQLIGQVNEAF
                     GANINMEQFFLTPCKQTVLAQLQVAAAADKA"
     source          1..6497
                     /db_xref="taxon:318"
                     /mol_type="genomic DNA"
                     /note="pathovar: glycinea"
                     /organism="Pseudomonas savastanoi pv. glycinea"
                     /plasmid="p4180A"
                     /strain="PG4180"
     subregion       1..6497
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="NRPS"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6497
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     aSDomain        13..165
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAQ93482.1_PP-binding.1"
                     /evalue="2.40E-14"
                     /label="AAQ93482.1_PP-binding.1"
                     /locus_tag="AAQ93482.1"
                     /protein_end="55"
                     /protein_start="4"
                     /score="45.6"
                     /tool="antismash"
                     /translation="KLDQIFEAIFQRPVENDEDIFDLGANSLTAIQLIGQVNEAFGANI
                     NMEQFF"
     CDS             242..1090
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /note="putative coronatine biosynthesis protein; protein
                     contains alpha/beta hydrolase fold; required for coronatine
                     production"
                     /product="CmaE"
                     /protein_id="AAQ93483.1"
                     /sec_met_domain="PF00561 (E-value: 2.2e-12, bitscore: 36.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHSLFTLNLDLERAGKVICAQSLPFDTARETLLLLSPVGTQCCYM
                     KNAALFLISHFNLIILESDTWLAYANEAGVNPEEGVADFIRQFNAALPEPVRVDALVGY
                     CSSAPLALLAANQGACRTLLLLNGAYFLKDDGVIKSQYERDVERMMQSIPQGNCAQVYE
                     AVSLLHTQSTYTPSDYRYQQVRPLRELSAFRQYLTFLNNLASLELVRIAQAVKTPTLVW
                     CGSQDRYTDTASSRYIAQLLPHSELVEDPDGQHHDFVDGHERLYLTMTRFLTRHKQRAI
                     Q"
     CDS             1087..2874
                     /NRPS_PKS="Domain: AMP-binding (14-409). E-value: 1e-82.
                     Score: 269.8. Matches aSDomain:
                     nrpspksdomains_AAQ93484.1_AMP-binding.1"
                     /NRPS_PKS="Domain: PP-binding (514-577). E-value: 8.1e-08.
                     Score: 24.6. Matches aSDomain:
                     nrpspksdomains_AAQ93484.1_PP-binding.1"
                     /codon_start=1
                     /function="activates and loads alloisoleucine for use in
                     coronatine biosynthesis"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     273.7; E-value: 4.1e-83)"
                     /gene_kind="biosynthetic"
                     /note="adenylation/thiolation didomain enzyme; required for
                     coronatine production"
                     /product="CmaA"
                     /protein_id="AAQ93484.1"
                     /sec_met_domain="AMP-binding (E-value: 2.1e-88, bitscore:
                     285.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.2e-08, bitscore:
                     24.6, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSYHSHPPKAYRRFESVCTQAPNAIAVVHEGKPVTYQQLQTQVL
                     ERSEALIRQGLADHPYMPLMANRCLEYLITMLACCKLGITYVSIEPSTPSKRLIAVLEQ
                     LGCNHLLLLGQPTDLRPDPTLTCFRLDDCGTLCSDGPALRQPIRRRLDDASVITVMFTS
                     GTTGVPKGVRISQDGLLNLVDNVQQQVQGKPRSYVHHSSIGFDAALFEVWVPLLTGACV
                     TLQPSEFNIDALDHCVRAASCDVLLLTTSLFHLVAQHRLSMLEAVRVLYVGGEVLKPVH
                     ARALLLANPRITLVNGYGPTENTVFSTWYSLNKPEDAERDVIPIGQFLHQVHGKIVDAK
                     LQEVEVGTPGELLLTGANLALGYLDEALTPTRFLQLPEGTYYRTGDYVIQDEHGMLFYQ
                     GRIDEQVKIKGFRVEIAEVEHALTQLPGVAQAVVQAHVMNDLENSLHAFIVFRHGSPTI
                     EESKLMSLLGDRLPHYMVPRRIHYLAELPLTANGKVDKRSLQPPEKAAVVSPQAGSAVL
                     EIWSGILGTRNLQLEHSIYGYGASSLSVVMAHSRINEILGRTTPFDEVARLSTFQEWVQ
                     YYATHADPVTSLRSQHGNH"
     CDS_motif       1117..1176
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AAQ93484.1_0001"
                     /evalue="7.00E+01"
                     /label="NRPS-A_a3"
                     /locus_tag="AAQ93484.1"
                     /protein_end="30"
                     /protein_start="10"
                     /score="-2.5"
                     /tool="antismash"
                     /translation="AYRRFESVCTQAPNAIAVVH"
     aSDomain        1129..2313
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAQ93484.1_AMP-binding.1"
                     /evalue="1.00E-82"
                     /label="AAQ93484.1_AMP-binding.1"
                     /locus_tag="AAQ93484.1"
                     /protein_end="409"
                     /protein_start="14"
                     /score="269.8"
                     /tool="antismash"
                     /translation="FESVCTQAPNAIAVVHEGKPVTYQQLQTQVLERSEALIRQGLADH
                     PYMPLMANRCLEYLITMLACCKLGITYVSIEPSTPSKRLIAVLEQLGCNHLLLLGQPTD
                     LRPDPTLTCFRLDDCGTLCSDGPALRQPIRRRLDDASVITVMFTSGTTGVPKGVRISQD
                     GLLNLVDNVQQQVQGKPRSYVHHSSIGFDAALFEVWVPLLTGACVTLQPSEFNIDALDH
                     CVRAASCDVLLLTTSLFHLVAQHRLSMLEAVRVLYVGGEVLKPVHARALLLANPRITLV
                     NGYGPTENTVFSTWYSLNKPEDAERDVIPIGQFLHQVHGKIVDAKLQEVEVGTPGELLL
                     TGANLALGYLDEALTPTRFLQLPEGTYYRTGDYVIQDEHGMLFYQGRIDEQVKIK"
     aSModule        1129..2817
                     /complete
                     /domains="nrpspksdomains_AAQ93484.1_AMP-binding.1"
                     /domains="nrpspksdomains_AAQ93484.1_PP-binding.1"
                     /locus_tags="AAQ93484.1"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       1561..1611
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AAQ93484.1_0002"
                     /evalue="7.10E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="AAQ93484.1"
                     /protein_end="175"
                     /protein_start="158"
                     /score="21.7"
                     /tool="antismash"
                     /translation="VMFTSGTTGVPKGVRIS"
     CDS_motif       2122..2205
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AAQ93484.1_0003"
                     /evalue="1.90E-08"
                     /label="NRPS-A_a6"
                     /locus_tag="AAQ93484.1"
                     /protein_end="373"
                     /protein_start="345"
                     /score="26.7"
                     /tool="antismash"
                     /translation="VGTPGELLLTGANLALGYLDEALTPTRF"
     CDS_motif       2278..2343
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AAQ93484.1_0004"
                     /evalue="3.10E-09"
                     /label="NRPS-A_a8"
                     /locus_tag="AAQ93484.1"
                     /protein_end="419"
                     /protein_start="397"
                     /score="29.0"
                     /tool="antismash"
                     /translation="YQGRIDEQVKIKGFRVEIAEVE"
     aSDomain        2629..2817
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAQ93484.1_PP-binding.1"
                     /evalue="8.10E-08"
                     /label="AAQ93484.1_PP-binding.1"
                     /locus_tag="AAQ93484.1"
                     /protein_end="577"
                     /protein_start="514"
                     /score="24.6"
                     /tool="antismash"
                     /translation="AVLEIWSGILGTRNLQLEHSIYGYGASSLSVVMAHSRINEILGRT
                     TPFDEVARLSTFQEWVQY"
     CDS             2861..3790
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /note="non-heme iron binding dioxygenase; similarity to
                     BarB1, BarB2 and SyB2; required for coronatine production"
                     /product="CmaB"
                     /protein_id="AAQ93485.1"
                     /sec_met_domain="PhyH (E-value: 6.1e-55, bitscore: 175.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEITDFSLTKQELQQFDRDGFIGPFTLYEPEEMKQMHKTIRAQLF
                     NRTHAPYDAPLDVAITNYDRHLDVDLLTSHVFRKEIVHRVRSILGPDVICWRSEFIPKY
                     PGNEGTDWHQADTFAHASGEPQLVWPEGEPFGGAINVWTGFTDASQENGCMQFIPGSHK
                     QMNYDETRGMTFDPVTNNNVVKGQYARGFNGYDYTTLQKDKSWKPDEASAVPIVMKAGQ
                     FVIFRSMLMHSSLPNSTTDKTRLGYVARYVPGRVKVYPDTDYVKEFGGEYRLDRFGVVQ
                     VAGTTTDPKNKVAIKSLSGMDLKPLVIY"
     CDS             3800..4222
                     /codon_start=1
                     /note="putative cyclase; similarity to methylmalonyl-CoA
                     epimerase; required for coronatine production"
                     /product="CmaC"
                     /protein_id="AAQ93486.1"
                     /transl_table=11
                     /translation="MEQPSYLAIDHIAYATRSTEKTSAFFTALGFEVLFHKQPIEKFGV
                     LITKLRSPAGEIVEVVEPFRPDSVVSRLLVNVEACIYHAAFRVRDIQTTQTSLASIGGV
                     SVTKPMTIPYPATEEHRSLTTSHMFHPAVGLFEITG"
     CDS             4232..4993
                     /NRPS_PKS="Domain: Thioesterase (24-234). E-value: 1.4e-33.
                     Score: 109.0. Matches aSDomain:
                     nrpspksdomains_AAQ93487.1_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1004:thioesterase (Score: 180.8; E-value: 3.8e-55)"
                     /gene_kind="biosynthetic-additional"
                     /note="thioesterase; required for coronatine production"
                     /product="CmaT"
                     /protein_id="AAQ93487.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2.3e-11, bitscore:
                     33.8, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADPFVVQRPWLAPLPGAPMNACRLLLCFPYGGGGASAFNGLSRL
                     AEVGVATWAVKLPGREDRSMEAPVTRVAHVIEAILDALQSVGMPYAFYGHSFGAGLALD
                     VTHALVERDRPLPTNLVLSGRMPPHTGYSPLLGAMDDNQLWQHVCSESLLPLPTDASCS
                     FARHALGKLKADLALNAQLTYRFIRPLPVPLYVLNGQDDPLLELHRLDEWQRYTSKSFH
                     SEYVPGGHFFFNINFQLLYLPLLTALDEQGS"
     aSDomain        4304..4933
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAQ93487.1_Thioesterase.1"
                     /evalue="1.40E-33"
                     /label="AAQ93487.1_Thioesterase.1"
                     /locus_tag="AAQ93487.1"
                     /protein_end="234"
                     /protein_start="24"
                     /score="109.0"
                     /tool="antismash"
                     /translation="LLLCFPYGGGGASAFNGLSRLAEVGVATWAVKLPGREDRSMEAPV
                     TRVAHVIEAILDALQSVGMPYAFYGHSFGAGLALDVTHALVERDRPLPTNLVLSGRMPP
                     HTGYSPLLGAMDDNQLWQHVCSESLLPLPTDASCSFARHALGKLKADLALNAQLTYRFI
                     RPLPVPLYVLNGQDDPLLELHRLDEWQRYTSKSFHSEYVPGGHFFFN"
     CDS_motif       4496..4558
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AAQ93487.1_0001"
                     /evalue="2.60E-05"
                     /label="NRPS-te1"
                     /locus_tag="AAQ93487.1"
                     /protein_end="109"
                     /protein_start="88"
                     /score="16.8"
                     /tool="antismash"
                     /translation="PYAFYGHSFGAGLALDVTHAL"
     CDS             4999..5235
                     /codon_start=1
                     /note="tRNA synthetase; similar to a portion of alanyl tRNA
                     synthetase; required for coronatine production"
                     /product="alanyl tRNA synthetase related protein"
                     /protein_id="AAQ93488.1"
                     /transl_table=11
                     /translation="MERVFWNTPYLSSLDACVLSIDGLDVVISPTIFFAEYGGQESDKG
                     CINRIEVTQATVVDERIIYRPCRRSRLCRGRSG"
     CDS             5643..6254
                     /codon_start=1
                     /note="putative amino acid efflux protein; probable
                     membrane transport protein; required for coronatine
                     production"
                     /product="CmaU"
                     /protein_id="AAQ93489.1"
                     /transl_table=11
                     /translation="MFNIMSVLGLLLLMPGPTNTLLLRSGALSGFRHAWSLSLLEYSAY
                     LLQISFWGYLLRHIGDSAPWCLKVVQLASMAYLFKTSHRLWRNPDNPCHSSPNVQISGM
                     YFFRLTLINPKGLLVVSFIAPEQTFTDIKLYLYFVAQLSMVVIPIGCAWVLLGAYIKRS
                     GHAWLTTLNINRTASVIICCFAVAILSQLTDSLIKTKLAF"
ORIGIN
        1 atgagctcag caaaactcga tcagatcttc gaggcaattt ttcagcgtcc ggtggaaaat
       61 gacgaagata tcttcgacct gggtgccaat tctcttaccg ctatccaatt gattggtcag
      121 gtcaacgagg cgtttggcgc caatatcaat atggagcagt tcttcttgac gccctgcaag
      181 caaacggtgc ttgcgcagtt gcaagtcgcc gctgcggcgg ataaagcctg agagggttgc
      241 catgcacagc ctgttcacac taaacctgga cctagagcga gccggcaagg tgatctgtgc
      301 ccagagcttg ccgttcgaca ctgcccggga aaccctccta ttgttatcgc ccgtgggcac
      361 ccagtgctgc tacatgaaga acgctgcact atttctgatc agccatttca atctgataat
      421 cctggaaagc gacacctggc tggcgtacgc caacgaagcc ggggtcaatc ccgaagaagg
      481 cgttgcggac ttcatccgcc agttcaatgc agcgctgcca gaacccgtgc gtgtagatgc
      541 actggtgggc tattgctcct cagcgccttt ggcactcttg gcagccaacc aaggtgcttg
      601 tcgcaccttg ctactgctta atggcgcgta ctttctcaaa gacgacgggg tgatcaagag
      661 ccaatacgag cgcgacgtgg agcgcatgat gcagtccatt ccccaaggca attgcgccca
      721 agtctacgaa gccgtgagtc tgttgcacac ccaaagcacc tataccccga gtgactatcg
      781 ctaccagcag gttcgccctc tgcgagaact gtctgcgttc cgccagtacc ttacgttcct
      841 caacaacctg gcgagcttgg aattggtcag aattgcgcag gccgtaaaga cccccacgct
      901 ggtgtggtgc ggcagccagg accgttatac cgacaccgca tcttcccggt acatcgccca
      961 gctgctgcct cacagtgaac tggtggaaga tcctgatggc cagcatcacg attttgtcga
     1021 tggccacgaa cgtttgtacc tgacgatgac ccgttttctg acccgtcaca agcagagggc
     1081 cattcaatga cctcctacca ttcacatcct cccaaagcct accgccgatt tgagtcggtc
     1141 tgcacgcaag cgccaaacgc aattgcggtg gtgcacgaag gaaaaccagt cacttatcaa
     1201 cagctccaga cgcaggtgtt ggaacgtagc gaagcactga tacgtcaagg cctggcagac
     1261 catccttaca tgccgctgat ggccaaccgg tgccttgagt atctgataac gatgctggcc
     1321 tgctgcaaac tgggaattac ctacgtctcc atcgagccaa gcacgccaag caaacgcctg
     1381 atcgccgtgc tggaacaatt gggctgcaat catttgctat tgctcggtca gccaacggac
     1441 ttgcgccctg acccaacgct aacatgcttt cgcctggacg actgcggcac tctgtgctcc
     1501 gacggccctg ccctacgcca gcctatccga cggcgtctgg atgatgcttc ggtgataacc
     1561 gtgatgttta cctctggcac taccggcgtc cccaaaggtg tacgcatcag ccaagacggg
     1621 ttgctgaatc tagtagacaa cgtacagcaa caggtccaag gcaagccccg cagctacgta
     1681 catcactcgt ccatcggctt cgatgctgca ttgtttgaag tgtgggtacc gctgctgaca
     1741 ggcgcctgcg tcaccctgca acctagcgag ttcaatatag atgcactgga tcactgcgta
     1801 cgcgcggcca gctgcgatgt gctgttactg accacctcac tgttccacct ggtggcgcaa
     1861 caccgccttt caatgctcga ggctgtgcgc gtgctctatg tcggcggtga agtacttaaa
     1921 ccggtgcatg cccgcgcgct gctgttggcc aatcctcgca ttaccctggt gaatggctac
     1981 ggcccgaccg agaatacagt gttctccact tggtacagtt tgaacaagcc tgaggacgcc
     2041 gagcgagacg taattcctat cgggcagttc ctacaccagg tgcacggcaa aatcgtcgat
     2101 gccaaactgc aagaggtgga ggtcggcacg cccggcgagt tgctgctcac aggtgcgaat
     2161 ctagccctag gttacctcga cgaagctttg accccaaccc gctttctaca actgccggaa
     2221 ggcacctatt accggaccgg cgactatgtc atccaagatg agcacggcat gcttttctac
     2281 caaggccgca ttgacgaaca ggtaaaaatc aaaggcttcc gggtggagat cgccgaggtc
     2341 gaacatgcgc tgacccaact gcctggcgtc gcccaggctg tggtgcaggc gcatgtcatg
     2401 aacgatttgg aaaacagcct acacgctttc atcgtgttcc ggcatggctc gccaactatc
     2461 gaagaaagca agctcatgag cctattgggc gaccggcttc ctcattacat ggtaccgagg
     2521 cggatccact acctggcgga acttccgctg acggccaatg gcaaagtcga caagcgttcc
     2581 ttacagccgc cggagaaagc agcggtggtg tcccctcagg ccggttcggc agtgctagag
     2641 atctggtccg gcatcctcgg cacccgcaac ctgcagttgg aacactcaat ttacggctat
     2701 ggcgcctcat ctttgagcgt ggtcatggcc catagccgca tcaacgaaat acttggcaga
     2761 acgacccctt tcgacgaagt cgccaggctg agcacctttc aagagtgggt gcagtactac
     2821 gcaacgcatg cagacccagt aacttctctc aggagccaac atggaaatca ctgacttttc
     2881 gttgacgaaa caggaacttc agcaatttga tcgagatggg ttcatcggtc ccttcacgct
     2941 ctatgagcct gaagagatga aacagatgca taagaccatc cgagcacaac tgttcaaccg
     3001 cacccatgct ccctacgacg cgccactgga tgtcgcgatc accaactacg accggcacct
     3061 ggatgtggac ttgctgacca gtcatgtgtt tcgcaaggaa atcgtgcatc gagtgcgaag
     3121 catcctcggc ccggatgtaa tctgctggcg cagcgagttc atccccaaat acccaggcaa
     3181 cgaaggcact gactggcacc aggcagacac tttcgctcac gcctccggcg agccgcaact
     3241 ggtgtggccc gagggcgagc cgttcggcgg cgctattaat gtgtggacgg gtttcactga
     3301 tgcctcccaa gaaaacggct gcatgcagtt catccccggc agccacaagc aaatgaacta
     3361 cgacgaaacc aggggcatga ccttcgaccc ggtcaccaac aataatgtgg tcaaggggca
     3421 gtatgcacgt ggattcaacg gctacgacta caccactctg caaaaggaca agtcatggaa
     3481 gccggatgaa gcttcggcag taccgatcgt gatgaaagcg ggccaatttg taatcttccg
     3541 ctcgatgctt atgcactcct cgctacccaa ttcgacaacc gacaagaccc gcctgggcta
     3601 tgtggcccgc tacgtacccg gacgggtcaa ggtttatcca gatacagatt acgttaagga
     3661 attcgggggc gagtatcgac ttgatcggtt cggcgtggtg caggtggccg gtaccaccac
     3721 cgaccccaag aacaaagtgg ccatcaagag cctaagcggc atggacctga agcccctagt
     3781 catttattga ggattgatca tggaacagcc ctcctacctg gcaatcgatc acatcgcgta
     3841 tgccacgcgg tctacggaga aaacctcggc gttcttcacg gccttggggt tcgaggtgct
     3901 gttccataaa caacccatcg aaaagtttgg cgtgttgata accaagctcc gctcgccagc
     3961 aggcgaaatc gtggaagtgg tcgaaccctt tcgacctgac agcgtggtca gccggctgct
     4021 ggtgaatgtg gaggcgtgta tataccatgc ggcctttagg gtccgcgata tccagactac
     4081 tcagacgtct ctggccagta tcggaggcgt ctcggtaacc aaacccatga ccattccgta
     4141 cccggccacc gaggaacatc gctcgctgac aacctcgcac atgttccacc ccgcagtggg
     4201 cctgttcgag atcaccggtt aaggagccgc catggccgat ccttttgtgg tgcaacggcc
     4261 ctggcttgca ccactgccag gcgcccctat gaacgcatgt cgcctgctgc tgtgtttccc
     4321 ctacggagga ggtggcgcct cagcgttcaa tggtttgagc cggctagccg aggtcggcgt
     4381 tgcgacatgg gcagtgaaac tacctggacg cgaggatcga agtatggaag cgccggtcac
     4441 gcgggtcgcg catgttatcg aggcgatcct cgatgcactg caaagcgtag gcatgccgta
     4501 cgcgttttac ggtcacagct tcggtgccgg gttggcgctt gatgtaaccc acgcgctagt
     4561 cgaacgcgac cggccgctgc cgacgaattt ggtcctgtcc gggcgcatgc cgccccatac
     4621 cggctactca ccgctactgg gtgcgatgga cgacaaccaa ttgtggcagc acgtatgctc
     4681 agagagcctc ttgccactgc cgacggatgc ctcgtgcagt ttcgccaggc atgcgctggg
     4741 caagctcaaa gccgacctag cgctcaatgc acaactcact tatcgcttta tccggccgct
     4801 gccagtgccc ctttacgttc tcaacggcca ggacgacccg ttgcttgagc tccatcgcct
     4861 agacgaatgg cagcgctaca ccagcaaaag cttccatagc gagtacgtac ccggcggaca
     4921 ttttttcttc aacatcaatt ttcagctttt gtacttgcct ctgttaaccg ctctagatga
     4981 acaaggcagt tgagcgttat ggaacgtgta ttttggaaca ccccttatct ttcgtcccta
     5041 gacgcttgcg tgctaagtat tgacgggctc gacgtggtga tcagcccgac gattttcttc
     5101 gccgaatacg gcggccaaga atcggataag ggttgcatca accgtatcga agtcacccag
     5161 gccacggttg ttgacgagcg catcatctat cggccttgcc gaagatccag actttgccgt
     5221 gggcgatctg gttagcgtgg agatcgattg gcctcgtcgg tatgcgctta tgcgtttgca
     5281 tttcgccgct gaaattgtgc tggagttaaa ttcccggact tggaaaagat cggtgcccat
     5341 atcggccaac acaaagcgcg gatcgacttc tcgctggagg gtaatgtcag ccagtacttt
     5401 ccggaaatcc tgcagggtgt agagcacctc gtagccgcct accgattgaa agccgcttta
     5461 cggacaccga gtgcttgcgc cgttgctggt acatcaaggg gttttcagag gtgccctgtg
     5521 gcggtacaca tctgcgtacc acgggcgagg tcgggcgtat tcggctcaag cgcaataaca
     5581 tcggcacgca caaagagcgt gtcgaaatct acctagtgga ttgaccgccc aggagagcta
     5641 tcatgttcaa catcatgtct gtacttgggt tgctgctctt gatgccaggg ccaacgaata
     5701 cattgctgct gcggtctgga gcactgagcg gattccgaca cgcctggtct ttgagcctgc
     5761 tggaatactc ggcctacctg ctgcagattt ccttttgggg gtatctgctc agacatatcg
     5821 gtgacagcgc tccctggtgc ctcaaggtgg tacaactggc gtcgatggct tatctgttca
     5881 aaacctcaca ccggctatgg cgcaaccccg ataacccttg ccattcctca cccaacgtgc
     5941 agatatccgg aatgtacttt tttcggctga ccctgatcaa ccccaagggt ctactggtgg
     6001 tttcgttcat cgctcccgag cagaccttta ccgatatcaa gttgtacctg tattttgtcg
     6061 cgcaactcag tatggtggta atcccgattg gctgtgcctg ggtgctgctt ggcgcataca
     6121 tcaaacgcag cgggcatgct tggctgacaa cgctgaatat taatcgcaca gcctcggtga
     6181 tcatttgctg cttcgcggtg gcgatccttt cccaattgac tgactcactg atcaagacca
     6241 aattggcatt ttaggtgtta gggagccgct gatttattcg atttttcgtc ccggcaacgc
     6301 tctggaggcc ttgatttatc tgggggcaac agctggattt tagaataaat cagcggctac
     6361 ttaggtcccg tgaagtagtg gtttggataa tgcttgcatc gttcggggtc tctctgccac
     6421 tgctcttgga taaacccaaa ggaggatatt ttgttgcagc aagcatgggc cgcataaagg
     6481 caaacaggtt agtacga
//
