BGC0000330: cupriachelin biosynthetic gene cluster from Cupriavidus necator H16
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Location: 1,886,949 - 1,923,310 nt. (total: 36,362 nt).
This entry is originally from NCBI GenBank AM260480.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000330
Short description cupriachelin biosynthetic gene cluster from Cupriavidus necator H16
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
Loci
AM260480.1
1886949 - 1923310
via Knock-out studies, Enzymatic assays, Heterologous expression
Compounds
  • cupriachelin
Species Cupriavidus necator H16 [taxonomy]
References
Chemical products information
cupriachelin Evidence:
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C33H57N7O16
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • H16_B1676
  • CAJ96461.1
  • h16_B1676
 1886949 - 1887290 (-) hypothetical membrane spanning protein
  • Transport
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  • H16_B1677
  • CAJ96462.1
  • h16_B1677
 1887274 - 1888905 (-) iron regulated protein
  • Transport
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  • H16_B1678
  • CAJ96463.1
  • h16_B1678
 1888902 - 1889270 (-) hypothetical protein
  • Transport
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  • H16_B1679
  • CAJ96464.1
  • fhuE
 1889296 - 1891578 (-) outer membrane receptor, TonB dependent
  • Transport
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  • H16_B1680
  • CAJ96465.1
  • pvdA
 1891684 - 1893084 (-) ornithine N-monooxygenase
  • Tailoring
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  • H16_B1681
  • CAJ96466.1
  • h16_B1681
 1893107 - 1894207 (-) N-acyltransferase
  • Tailoring
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  • H16_B1682
  • CAJ96467.1
  • h16_B1682
 1894240 - 1895922 (-) ABC-type transporter, ATPase and permease components: Pep3E family
  • Transport
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  • H16_B1683
  • CAJ96468.1
  • h16_B1683
 1895919 - 1899146 (-) non-ribosomal peptide synthetase
  • Scaffold biosynthesis
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  • H16_B1684
  • CAJ96469.1
  • fhuF
 1899397 - 1900152 (+) putative iron transport-related membrane protein
  • Transport
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  • H16_B1685
  • CAJ96470.1
  • nrpC
 1900165 - 1906191 (-) non-ribosomal peptide synthetase
  • Scaffold biosynthesis
  • Knock-out
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  • H16_B1686
  • CAJ96471.1
  • nrpB
 1906229 - 1909324 (-) non-ribosomal peptide synthetase
  • Scaffold biosynthesis
  • Activity assay
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  • H16_B1687
  • CAJ96472.1
  • nrpA
 1909317 - 1918601 (-) non-ribosomal peptide synthetase
  • Scaffold biosynthesis
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  • H16_B1688
  • CAJ96473.1
  • h16_B1688
 1918717 - 1919778 (-) pyoverdine biosynthesis regulatory gene
  • Tailoring
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  • H16_B1689
  • CAJ96474.1
  • mbtH
 1920048 - 1920254 (+) MbtH-like protein
  • Activation / processing
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  • H16_B1690
  • CAJ96475.1
  • h16_B1690
 1920251 - 1921069 (+) thioesterase
  • Activation / processing
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  • H16_B1691
  • CAJ96476.1
  • h16_B1691
 1921056 - 1921889 (+) phosphopantetheinyltransferase family protein
  • Activation / processing
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  • H16_B1692
  • CAJ96477.1
  • h16_B1692
 1921886 - 1923310 (+) aminotransferase
  • Tailoring
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Biosynthesis information

Biosynthetic modules

Name
1
Type
nrps-type1
Genes
nrpA
Substrates
aspartic acid (evidence: Structure-based inference)
Integrated Monomers
Domains
condensation (LCL), adenylation, oxidase
Name
2
Type
nrps-type1
Genes
nrpA
Substrates
2,4-diaminobutyric acid (evidence: Structure-based inference)
Integrated Monomers
Domains
condensation (LCL), adenylation, oxidase
Name
3
Type
nrps-type1
Genes
nrpB
Substrates
aspartic acid (evidence: Activity assay)
Integrated Monomers
Domains
condensation (LCL), adenylation
Name
4
Type
nrps-type1
Genes
h16_B1683
Substrates
glycine (evidence: Sequence-based prediction)
Integrated Monomers
Domains
condensation (LCL), adenylation
Name
5
Type
nrps-type1
Genes
nrpC
Substrates
N5-hydroxyornithine (evidence: Sequence-based prediction)
Integrated Monomers
Domains
condensation (LCL), adenylation
Annotation changelog

Entry version: 4

Date
Changes
Submitters
Reviewers
2022-10-07
Update chemical activity to schema version 2.11
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 3

Date
Changes
Submitters
Reviewers
2022-09-15
Corrected NRP module activity
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
2022-09-15
Updated NRP substrate specificities
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 2

Date
Changes
Submitters
Reviewers
2019-10-16
Migrated from v1.4
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
2019-10-16
Updated compound(s) information (NPAtlas curation)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 1

Date
Changes
Submitters
Reviewers
2015-06-12
Submitted
  • (ID: YETYK72PRB3MT456E3R3XHBM)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
Detailed domain annotation
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