BGC0000332 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000332 (Microcystis sp. NIVA-CYA 172/5)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS	1	31,880	cyanopeptolin	NRPS:Type I	100%

BGC0000332 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 31,880 nt. (total: 31,880 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 31,881
Label		BGC0000332

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000332	1.00	NRP	cyanopeptolin	Microcystis sp. NIVA-CYA 172/5
BGC0000331	0.46	NRP	cyanopeptolin	Planktothrix agardhii NIVA-CYA 116
BGC0001018	0.44	NRP	micropeptin K139	Microcystis aeruginosa K-139
BGC0000426	0.39	NRP	sevadicin	Paenibacillus larvae
BGC0001844	0.38	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001050	0.37	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0001131	0.36	NRP	ambactin	Xenorhabdus miraniensis
BGC0002135	0.36	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833	0.35	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758	0.35	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000332	1.00	NRP	cyanopeptolin	Microcystis sp. NIVA-CYA 172/5
BGC0000331	0.76	NRP	cyanopeptolin	Planktothrix agardhii NIVA-CYA 116
BGC0001018	0.75	NRP	micropeptin K139	Microcystis aeruginosa K-139
BGC0000426	0.71	NRP	sevadicin	Paenibacillus larvae
BGC0001844	0.70	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001050	0.70	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0001131	0.69	NRP	ambactin	Xenorhabdus miraniensis
BGC0002135	0.69	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833	0.68	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758	0.68	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 31880:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gln) + (Thr) + (Ile - X - Phe - NMe-Tyr) + (Ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

mcnA

Search NORINE for peptide: strict or relaxed

AMP-binding (477..877): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQFGLIDK
Nearest Stachelhaus code(s):: Gln DAWQFGLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mcnB

Search NORINE for peptide: strict or relaxed

AMP-binding (476..909): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mcnC

Search NORINE for peptide: strict or relaxed

AMP-binding (469..881): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQDLGVVDK
Nearest Stachelhaus code(s):: Ile DAQDLGVVDK (100% 8Å match); Gln or Glu DAQDLGVVDK (91% 8Å match); Gln DAQDLGVVDK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1551..1958): X

nrpys: Ahp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVENAGVVTK
Nearest Stachelhaus code(s):: Ahp DVENAGVVTK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2619..3024): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTIAAVCK
Nearest Stachelhaus code(s):: Phe DAWTIAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3683..4086): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DASTIAGVCK
Nearest Stachelhaus code(s):: Tyr DASTIAGVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mcnE

Search NORINE for peptide: strict or relaxed

AMP-binding (539..952): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGVTFK
Nearest Stachelhaus code(s):: Ile DAFFLGVTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)