BGC0000335 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000335 (Cystobacter fuscus)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	37,509	cystomanamide A/cystomanamide B/cystomanamide C/cystomanamide D	NRPS:Type I+PKS	100%

BGC0000335 - Region 1 - NRPS,T1PKS

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Location: 1 - 37,509 nt. (total: 37,509 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 37,510
Label		BGC0000335

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000335	2.00	NRP, Polyketide	cystomanamide A, cystomanamide B, cystomanamide C, cystomanamide D	Cystobacter fuscus
BGC0002071	0.67	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0000343	0.63	NRP	enterobactin	Pseudomonas sp. J465
BGC0001133	0.62	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000457	0.60	NRP	vicibactin	Rhizobium etli CFN 42
BGC0000426	0.60	NRP	sevadicin	Paenibacillus larvae
BGC0002437	0.60	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001629	0.59	NRP	jessenipeptin	Pseudomonas sp. QS1027
BGC0001671	0.58	NRP	monobactam	Agrobacterium tumefaciens
BGC0001434	0.57	NRP	nematophin	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000335	1.00	NRP, Polyketide	cystomanamide A, cystomanamide B, cystomanamide C, cystomanamide D	Cystobacter fuscus
BGC0002071	0.67	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0000343	0.65	NRP	enterobactin	Pseudomonas sp. J465
BGC0001133	0.65	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000457	0.64	NRP	vicibactin	Rhizobium etli CFN 42
BGC0000426	0.64	NRP	sevadicin	Paenibacillus larvae
BGC0002437	0.63	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001629	0.63	NRP	jessenipeptin	Pseudomonas sp. QS1027
BGC0001671	0.63	NRP	monobactam	Agrobacterium tumefaciens
BGC0001434	0.62	NRP	nematophin	Xenorhabdus budapestensis

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 37509:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - D-Asn) + (Asn - D-X) + (D-Tyr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctmA

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CAL_domain (35..476): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 160.1
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0
NH2: -4.0

PKS_AT (1208..1501): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 66.7%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 167.4
inactive: 99.3
Methoxymalonyl-CoA: 83.2
Methylmalonyl-CoA: 71.0
Propionyl-CoA: 45.2
Acetyl-CoA: 42.5
Ethylmalonyl-CoA: 41.8
Benzoyl-CoA: 37.5
Isobutyryl-CoA: 36.0
CHC-CoA: 32.3
2-Methylbutyryl-CoA: 30.4
fatty_acid: 26.1
trans-1,2-CPDA: 18.8
3-Methylbutyryl-CoA: 12.4

AMP-binding (2675..3072): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DMTKVGEVGK
Nearest Stachelhaus code(s):: Asn DMTKVGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctmC

Search NORINE for peptide: strict or relaxed

AMP-binding (457..852): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1504..1892): X

nrpys: S-bohPhe, Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: DALIVAAVCK
Nearest Stachelhaus code(s):: S-bohPhe or Phe DALIVAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctmD

Search NORINE for peptide: strict or relaxed

AMP-binding (1636..2026): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTVAAVCK
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)