BGC0000337: destruxin A biosynthetic gene cluster from Metarhizium robertsii ARSEF 23
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Location: 1 - 106,198 nt. (total: 106,198 nt).
This entry is originally from NCBI GenBank GL698760.1, but has been modified (see Modifications tab for details).

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
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Gene details
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General
Compounds
Genes
NRP
History
NRPS/PKS domains
KnownClusterBlast
Modifications
General information about the BGC
MIBiG accession BGC0000337
Short description destruxin A biosynthetic gene cluster from Metarhizium robertsii ARSEF 23
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "The metabolite destruxins are used by the fungus to evade insect host immunities"
Biosynthetic class(es)
  • NRP
Loci NCBI GenBank: GL698760.1
Compounds
  • destruxin A
Species Metarhizium robertsii ARSEF 23 [taxonomy]
References
Chemical products information
destruxin A
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C29H47N5O7
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Comment Extra
  • MAA_10031
 1051 - 2514 (+) benzoylformate decarboxylase
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  • MAA_10032
 4573 - 6316 (-) laccase Lcc4
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  • MAA_10033
 7766 - 12927 (-) polyketide synthase, putative
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  • MAA_10034
 14151 - 16040 (+) cytochrome P450, putative
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  • MAA_10035
 16872 - 18679 (+) cytochrome P450 monooxygenaseGliC-like, putative
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  • MAA_10036
 19976 - 26899 (+) nonribosomal peptide synthase GliP-like, putative
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  • MAA_10037
 27748 - 29731 (+) Cytochrome P450
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  • MAA_10038
 30537 - 32991 (+) GPI-anchor biosynthetic protein (Mcd4), putative
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  • MAA_10039
 34068 - 35224 (-) subgroup IIIi aminotransferase, putative
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  • MAA_10040
 35733 - 36524 (-) SirZ; zinc finger transcriptional regulator
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  • MAA_10041
 41364 - 43043 (-) proteinase, putative
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  • MAA_10042
 46696 - 47655 (+) phytanoyl-CoA dioxygenase family protein
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  • MAA_10043
 47923 - 71714 (-) non-ribosomal peptide synthetase
  • Scaffold biosynthesis
Knock-out
Biosynthesis of the first NRP dtx B.
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  • MAA_10044
 72959 - 74873 (-) benzoate 4-monooxygenase cytochrome P450
  • Tailoring (Oxidation)
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metabolite hydroxylation, desaturation, oxidation, and/or epoxidation
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  • MAA_10045
 76321 - 77803 (+) aldo-keto reductase
  • Precursor biosynthesis
Knock-out
Conversion of alpha-ketoisocaproic acid into alpha-hydroxyisocaproic acid
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  • MAA_10046
 78302 - 79789 (+) glutamate decarboxylase
  • Precursor biosynthesis
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Decarboxylation of aspartic acid into beta-Alanine
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  • MAA_10047
 80445 - 81634 (-) Vacuolar ATP synthase subunit B
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  • MAA_10048
 84098 - 88361 (+) multidrug resistance protein
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  • MAA_10049
 95996 - 96244 (+) sterigmatocystin 8-O-methyltransferase precursor
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  • MAA_10050
 100674 - 102636 (+) vitamin H transporter, putative
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  • MAA_10051
 104003 - 104779 (-) hypothetical protein
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NRP-specific information
Subclass N/A
Cyclic? no
Release type
  • Hydrolysis
NRP-synthases
Gene Modules
MAA_10043
Module ?
Specificity: beta-alanine
Evidence for specificity:
  • Structure-based inference
Module ?
Specificity: 2-hydroxypent-4-enoic acid / 2-hydroxy-4-methylpentanoic acid / 2,4-dihydroxypentanoic acid / 2,5-dihydroxy-4-methylpentanoic acid / 2-hydroxy-4-methylpentanedioic acid
Evidence for specificity:
  • Structure-based inference
  • Mass spectrometry
Module ?
Specificity: proline
Evidence for specificity:
  • Structure-based inference
Module ?
Specificity: isoleucine / valine
Evidence for specificity:
  • Structure-based inference
  • Mass spectrometry
Module ?
Specificity: valine
Evidence for specificity:
  • Structure-based inference
Module ?
Specificity: alanine
Evidence for specificity:
  • Structure-based inference
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: XVPU6C2LIDSVZRMGJYGDLQKD, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Removed duplicate gene annotation
  • Added NRP substrate specificities
Detailed domain annotation
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Modifications to original record
  • MAA_10031 was added
  • MAA_10032 was added
  • MAA_10033 was added
  • MAA_10034 was added
  • MAA_10035 was added
  • MAA_10036 was added
  • MAA_10037 was added
  • MAA_10038 was added
  • MAA_10039 was added
  • MAA_10040 was added
  • MAA_10041 was added
  • MAA_10042 was added
  • MAA_10043 was added
  • MAA_10044 was added
  • MAA_10045 was added
  • MAA_10046 was added
  • MAA_10047 was added
  • MAA_10048 was added
  • MAA_10049 was added
  • MAA_10050 was added
  • MAA_10051 was added