BGC0000337 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000337 (Metarhizium robertsii ARSEF 23)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS	1	106,198	High	destruxin A	NRPS:Type I

BGC0000337 - Region 1 - NRPS

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Location: 1 - 106,198 nt. (total: 106,198 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 106,199
Label		BGC0000337

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase oxygenase

MAA_10031: pyruvate oxidase/decarboxylase

smcogs: SMCOG1055: pyruvate oxidase/decarboxylase

MAA_10033: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

MAA_10034: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

MAA_10035: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

MAA_10037: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

MAA_10044: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

MAA_10045: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: acyltransferase

MAA_10043: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

Lyases: decarboxylase

MAA_10046: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Ligases: ligase

MAA_10036: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000337.4	1.02	NRPS	destruxin A	Metarhizium robertsii ARSEF 23
BGC0001135.5	0.70	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001641.4	0.58	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000342.4	0.45	NRPS	enniatin	Fusarium equiseti
BGC0001261.3	0.42	NRPS	AM-toxin	Alternaria alternata
BGC0001240.4	0.38	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0000307.4	0.37	NRPS	AbT1	Aureobasidium pullulans
BGC0001132.4	0.37	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001166.5	0.36	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000337.4	0.99	NRPS	destruxin A	Metarhizium robertsii ARSEF 23
BGC0000342.4	0.75	NRPS	enniatin	Fusarium equiseti
BGC0001261.3	0.73	NRPS	AM-toxin	Alternaria alternata
BGC0002135.2	0.73	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001240.4	0.72	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0000307.4	0.71	NRPS	AbT1	Aureobasidium pullulans
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 91714:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Leu) + (bAla - D-X - Pro - Ile - NMe-Val - NMe-Ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 46899:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Leu)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 27922 - 91714:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (bAla - D-X - Pro - Ile - NMe-Val - NMe-Ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MAA_10036: X - Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (14..409): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAAVIGAIMK
Nearest Stachelhaus code(s):: Leu DAWIIGAIVK (41% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1109..1436): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DILILGGVAK
Nearest Stachelhaus code(s):: Leu DIFALGGVAK (47% 8Å match)
Stachelhaus code match:: 80% (moderate)

MAA_10043: bAla - X - Pro - Ile - Val - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (262..662): bAla

nrpys: bAla

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DIFYAIATAK
Nearest Stachelhaus code(s):: bAla DIFYAIATAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1331..1740): X

nrpys: Hpea, dohPen

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GANLIGATVK
Nearest Stachelhaus code(s):: Hpea or dohPen GANLIGATVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2862..3255): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLHEIGIISK
Nearest Stachelhaus code(s):: Pro DLHEIGIISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3923..4321): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DGLFIGIPVK
Nearest Stachelhaus code(s):: Ile DGLFIGIPVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4990..5390): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFYGGTFK
Nearest Stachelhaus code(s):: Val DAWFYGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6465..6860): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVWIYAAVIK
Nearest Stachelhaus code(s):: Ala DVWIYAAVIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor