Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000338.1:
Cluster 1NRP1120292
The following clusters are from record BGC0000338.2:
Cluster 2NRP147514

BGC0000338, echinocandin B biosynthetic gene cluster from Aspergillus rugulosus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Echinocandin B
PubChem ID: 456858
SMILES string: Copy to clipboard
Molecular formula: C53H88N8O16
Average molecular mass: 1093.327 Da
Molecular activity: Antifungal
Molecular target: 1,3-beta-D-glucan synthase

Class-specific details

Biosynthetic class(es):
NRP
Nonribosomal peptide subclass:
Other lipopeptide (cyclic)
linoleic acid
Thioesterase type:
None
Release / cyclization type:
Macrolactamization

Nonribosomal peptide synthetases:
ecdA (AFT91378.1)
Module 0
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: N/A
Module 1
A specificity: Ornithine
Evidence for specificity: Activity assay
C domain subtype: Starter
Module 2
A specificity: Threonine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 3
A specificity: Proline
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 4
A specificity: Homotyrosine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 5
A specificity: Threonine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 6
A specificity: 4-Methylproline
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 7
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL

Gene cluster description

echinocandin B (BGC0000338). Gene Cluster 1. Biosynthetic class = NRP. GenBank JX421684, positions 20000-81000. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays
MIxS-compliance:Unknown
Contact for this cluster:Yit-Heng Chooi (The Australian National University)

Literature references

1. Cacho RA et al. (2012) Identification and characterization of the echinocandin B biosynthetic gene cluster from Emericella rugulosa NRRL 11440. J Am Chem Soc 134(40):16781-90. doi: 10.1021/ja307220z. Epub 2012
2. Jiang W et al. (2013) EcdGHK are three tailoring iron oxygenases for amino acid building blocks of the echinocandin scaffold. J Am Chem Soc 135(11):4457-66. doi: 10.1021/ja312572v. Epub 2013 Mar
3. Sawistowska-Schroder ET et al. (1984) Echinocandin inhibition of 1,3-beta-D-glucan synthase from Candida albicans. FEBS Lett 173(1):134-8.
4. Nyfeler R, Keller-Schierlein W. (1974 TI) [Metabolites of microorganisms. 143. Echinocandin B, a novel polypeptide-antibiotic from Aspergillus nidulans var. echinulatus: isolation and structural components]. Helv Chim Acta 57(8):2459-77. doi: 10.1002/hlca.19740570818.

BGC0000338, echinocandin B biosynthetic gene cluster from Aspergillus rugulosus. Locus 2. Full MIBiG entry.

Chemical compounds

Compound: Echinocandin B
PubChem ID: 456858
SMILES string: Copy to clipboard
Molecular formula: C53H88N8O16
Average molecular mass: 1093.327 Da
Molecular activity: Antifungal
Molecular target: 1,3-beta-D-glucan synthase

Class-specific details

Biosynthetic class(es):
NRP
Nonribosomal peptide subclass:
Other lipopeptide (cyclic)
linoleic acid
Thioesterase type:
None
Release / cyclization type:
Macrolactamization

Nonribosomal peptide synthetases:
ecdA (AFT91378.1)
Module 0
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: N/A
Module 1
A specificity: Ornithine
Evidence for specificity: Activity assay
C domain subtype: Starter
Module 2
A specificity: Threonine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 3
A specificity: Proline
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 4
A specificity: Homotyrosine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 5
A specificity: Threonine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 6
A specificity: 4-Methylproline
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 7
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL

Gene cluster description

echinocandin B (BGC0000338). Gene Cluster 2. Biosynthetic class = NRP. GenBank JX421685, positions 21000-38000. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays
MIxS-compliance:Unknown
Contact for this cluster:Yit-Heng Chooi (The Australian National University)

Literature references

1. Cacho RA et al. (2012) Identification and characterization of the echinocandin B biosynthetic gene cluster from Emericella rugulosa NRRL 11440. J Am Chem Soc 134(40):16781-90. doi: 10.1021/ja307220z. Epub 2012
2. Jiang W et al. (2013) EcdGHK are three tailoring iron oxygenases for amino acid building blocks of the echinocandin scaffold. J Am Chem Soc 135(11):4457-66. doi: 10.1021/ja312572v. Epub 2013 Mar
3. Sawistowska-Schroder ET et al. (1984) Echinocandin inhibition of 1,3-beta-D-glucan synthase from Candida albicans. FEBS Lett 173(1):134-8.
4. Nyfeler R, Keller-Schierlein W. (1974 TI) [Metabolites of microorganisms. 143. Echinocandin B, a novel polypeptide-antibiotic from Aspergillus nidulans var. echinulatus: isolation and structural components]. Helv Chim Acta 57(8):2459-77. doi: 10.1002/hlca.19740570818.