Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000345.1:
Cluster 1NRP / Polyketide125783

BGC0000345, eponemycin biosynthetic gene cluster from Streptomyces hygroscopicus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: eponemycin
PubChem ID: 196802
SMILES string: Copy to clipboard
Molecular formula: C20H34N2O6
Average molecular mass: 398.5 Da
Molecular activity: Cytotoxic, Inhibitor
Molecular target: proeasome

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
Other (linear)
Thioesterase type:
None
Release / cyclization type:
Unknown

Nonribosomal peptide synthetases:
epnJ (AHB38511.1)
Module X
A specificity: Leucine
Evidence for specificity: Sequence-based prediction
C domain subtype: N/A
epnG (AHB38515.1)
Module 1
A specificity: Serine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 2
A specificity: Leucine
Evidence for specificity: None
C domain subtype: LCL
Polyketide subclass:
Other (linear)
Polyketide synthase subclass:
Modular type I
Starter unit:
Other
Thioesterase type:
Type I
Release / cyclization type:
Unknown

Modular polyketide synthases:
epnH (AHB38509.1)
Module 3
AT specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Scaffold-modifying domain: Methylation

Gene cluster description

eponemycin (BGC0000345). Gene Cluster 1. Biosynthetic class = NRP/Polyketide. GenBank KF647220, positions 857-24594. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Heterologous expression
MIxS-compliance:Unknown
Contact for this cluster:Leonard Kaysser (Eberhard Karls University Tuebingen)

Literature references

1. Schorn M et al. (2014) Genetic basis for the biosynthesis of the pharmaceutically important class of epoxyketone proteasome inhibitors. ACS Chem Biol 9(1):301-9. doi: 10.1021/cb400699p. Epub 2013 Nov 8.
2. Sugawara K et al. (1990) Eponemycin, a new antibiotic active against B16 melanoma. I. Production, isolation, structure and biological activity. J Antibiot (Tokyo) 43(1):8-18.
3. Meng L et al. (1999) Eponemycin exerts its antitumor effect through the inhibition of proteasome function. Cancer Res 59(12):2798-801.